scholarly journals Ca2+ signalling in mouse urethral smooth muscle in situ : role of Ca2+ stores and Ca2+ influx mechanisms

2018 ◽  
Vol 596 (8) ◽  
pp. 1433-1466 ◽  
Author(s):  
Bernard T. Drumm ◽  
Benjamin E. Rembetski ◽  
Caroline A. Cobine ◽  
Salah A. Baker ◽  
Gerard P. Sergeant ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urethral Smooth Muscle

scholarly journals Different role of muscarinic acetylcholine receptors (mAChR) in the urethral smooth muscle of male rabbit

1997 ◽  
Vol 73 ◽  
pp. 281
Author(s):  
Katsushi Nagahama ◽  
Toshihiko Tsujii ◽  
Hiroshi Azuma ◽  
Takashi Morita ◽  
Hiroyuki Oshima
Keyword(s):  
Smooth Muscle ◽  
Acetylcholine Receptors ◽  
Muscarinic Acetylcholine Receptors ◽  
Male Rabbit ◽  
Muscarinic Acetylcholine ◽  
Urethral Smooth Muscle

scholarly journals In Situ Estrogen Synthesized by Aromatase P450 in Uterine Leiomyoma Cells Promotes Cell Growth Probably Via an Autocrine/Intracrine Mechanism*

Endocrinology ◽  
2000 ◽  
Vol 141 (10) ◽  
pp. 3852-3861 ◽  
Author(s):  
Hiroshi Sumitani ◽  
Makio Shozu ◽  
Tomoya Segawa ◽  
Kouichi Murakami ◽  
Hui-Juan Yang ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Cell Growth ◽  
Growth Promotion ◽  
Reproductive Age ◽  
Interleukin 1Β ◽  
Minimum Concentration ◽  
A Cell ◽  
Strong Immunoreactivity

Abstract In the present study we characterized in detail the expression of aromatase P450 in leiomyomas to determine the role of in situ estrogen in the growth advantage of leiomyomas. The levels of aromatase P450 transcripts were determined by quantitative RT-PCR to be significantly higher in leiomyomas than in corresponding myometrium. The overexpression of aromatase P450 in leiomyomas was also confirmed by Western blot analysis. The estimated size of immunoreactive aromatase was 58 kDa, similar to that in placenta. To identify a cell type that express aromatase P450 in leiomyomas, histological specimens were stained for aromatase P450 using a polyclonal antibody. Strong immunoreactivity was detected in the cytoplasm of leiomyoma cells, whereas surrounding normal myometrium displayed weak or negative staining. Smooth muscle-like cells in culture obtained from leiomyomas, positive for actin D fiber, possessed immunoreactive granules of aromatase in the cytoplasm. Conversion of androgen to estrogen was effectively stimulated by phorbol myristate acetate and dexamethasone plus interleukin-1β and was completely abolished by selective inhibitors of aromatase P450 (fadrozole and TZA-2209), but not by inhibitors of 5α-reductase (finasteride and flutamide). The apparent Km of androstenedione was 3 nm in the presence of dexamethasone and interleukin-1β, corresponding to the plasma concentration of androstenedione in women of reproductive age. To determine whether endogenous aromatase P450 plays a role in the growth promotion of leiomyoma cells, we evaluated the cell growth of smooth muscle-like cells treated with various concentrations of estrogen and androgen using a WST-1 assay. Treatment with testosterone (10−8 and 10−7m) and androstenedione (10−8 and 10−7m) stimulated the growth of smooth muscle-like cells obtained from leiomyomas to the same extent as estradiol (10−10–10−7m), whereas dihydrotestosterone (10−11–10−8m) did not. The stimulatory effect of testosterone on cell growth was again abolished by cotreatment with fadrozole. The level of estradiol in the medium of testosterone (10−8m)-treated smooth muscle-like cells was 10−11m, which was 1 order lower than the minimum concentration of estradiol necessary to promote cell growth (10−10m). This indicates that estradiol synthesized in leiomyomas promotes their growth via an autocrine/intracrine mechanism. We conclude that myometrial cells of leiomyomas overexpress aromatase P450 and are able to synthesize sufficient estrogen to accelerate their own cell growth. Overexpression of aromatase P450 may play a role in the growth advantage of leiomyoma tissue over surrounding myometrium via an autocrine/intracrine mechanism.

276 Does TRP channel play a role of extracellular calcium sensing in urethral smooth muscle?

2016 ◽  
Vol 15 (3) ◽  
pp. e276
Author(s):  
S. Kajioka ◽  
M. Hayashi ◽  
T. Maki ◽  
R. Takahashi ◽  
M. Etoh
Keyword(s):  
Smooth Muscle ◽  
Extracellular Calcium ◽  
Trp Channel ◽  
Calcium Sensing ◽  
Urethral Smooth Muscle

The Functional Role of β-Adrenoceptor Subtypes in Mediating Relaxation of Pig Urethral Smooth Muscle

2003 ◽  
Vol 170 (6) ◽  
pp. 2508-2511 ◽  
Author(s):  
TOMONORI YAMANISHI ◽  
CHRISTOPHER R. CHAPPLE ◽  
KOSAKU YASUDA ◽  
KEN-ICHIRO YOSHIDA ◽  
RUSSELL CHESS-WILLIAMS
Keyword(s):  
Smooth Muscle ◽  
Functional Role ◽  
Urethral Smooth Muscle

Contribution of Cav1.2 Ca2+ channels and store-operated Ca2+ entry to pig urethral smooth muscle contraction

2020 ◽  
Vol 318 (2) ◽  
pp. F496-F505
Author(s):  
Benjamin E. Rembetski ◽  
Kenton M. Sanders ◽  
Bernard T. Drumm
Keyword(s):  
Smooth Muscle ◽  
Electrical Field Stimulation ◽  
Smooth Muscle Contraction ◽  
Isometric Tension ◽  
Electrical Field ◽  
Voltage Dependent ◽  
Urethral Smooth Muscle ◽  
Evoked Contractions ◽  
Ca2 Channels

Urethral smooth muscle (USM) generates tone to prevent urine leakage from the bladder during filling. USM tone has been thought to be a voltage-dependent process, relying on Ca2+ influx via voltage-dependent Ca2+ channels in USM cells, modulated by the activation of Ca2+-activated Cl− channels encoded by Ano1. However, recent findings in the mouse have suggested that USM tone is voltage independent, relying on Ca2+ influx through Orai channels via store-operated Ca2+ entry (SOCE). We explored if this pathway also occurred in the pig using isometric tension recordings of USM tone. Pig USM strips generated myogenic tone, which was nearly abolished by the Cav1.2 channel antagonist nifedipine and the ATP-dependent K+ channel agonist pinacidil. Pig USM tone was reduced by the Orai channel blocker GSK-7975A. Electrical field stimulation (EFS) led to phentolamine-sensitive contractions of USM strips. Contractions of pig USM were also induced by phenylephrine. Phenylephrine-evoked and EFS-evoked contractions of pig USM were reduced by ~50–75% by nifedipine and ~30% by GSK-7975A. Inhibition of Ano1 channels had no effect on tone or EFS-evoked contractions of pig USM. In conclusion, unlike the mouse, pig USM exhibited voltage-dependent tone and agonist/EFS-evoked contractions. Whereas SOCE plays a role in generating tone and agonist/neural-evoked contractions in both species, this dominates in the mouse. Tone and agonist/EFS-evoked contractions of pig USM are the result of Ca2+ influx primarily through Cav1.2 channels, and no evidence was found supporting a role of Ano1 channels in modulating these mechanisms.

scholarly journals AB313. SPR-40 Spontaneous Ca2+ waves in mouse urethral smooth muscle visualized with a genetically encoded Ca2+ indicator in situ

2016 ◽  
Vol 5 (S2) ◽  
pp. AB313-AB313
Author(s):  
Bernard T. Drumm ◽  
Sean M. Ward ◽  
Kenton M. Sanders
Keyword(s):  
Smooth Muscle ◽  
Urethral Smooth Muscle

Role of K + Channels in Regulating Spontaneous Activity in Detrusor Smooth Muscle In Situ in the Mouse Bladder

2009 ◽  
Vol 181 (5) ◽  
pp. 2355-2365 ◽  
Author(s):  
Masa Hayase ◽  
Hikaru Hashitani ◽  
Kenjiro Kohri ◽  
Hikaru Suzuki
Keyword(s):  
Smooth Muscle ◽  
Spontaneous Activity ◽  
Detrusor Smooth Muscle ◽  
K Channels

Electron Probe Analysis of Muscle

1982 ◽  
Vol 40 ◽  
pp. 398-401
Author(s):  
A. V. Somlyo ◽  
H. Shuman ◽  
A. P. Somlyo
Keyword(s):  
Skeletal Muscle ◽  
Smooth Muscle ◽  
Sarcoplasmic Reticulum ◽  
Resting State ◽  
Binding Sites ◽  
Electron Probe ◽  
Frog Skeletal Muscle ◽  
Electron Probe Analysis ◽  
Probe Analysis

Electron probe analysis of frozen dried cryosections of frog skeletal muscle, rabbit vascular smooth muscle and of isolated, hyperpermeab1 e rabbit cardiac myocytes has been used to determine the composition of the cytoplasm and organelles in the resting state as well as during contraction. The concentration of elements within the organelles reflects the permeabilities of the organelle membranes to the cytoplasmic ions as well as binding sites. The measurements of [Ca] in the sarcoplasmic reticulum (SR) and mitochondria at rest and during contraction, have direct bearing on their role as release and/or storage sites for Ca in situ.

The role of Na+/Ca2+ exchanger in opossum esophageal smooth muscle contractillty

2001 ◽  
Vol 120 (5) ◽  
pp. A57-A57 ◽  
Author(s):  
P ROY ◽  
Y ZHANG ◽  
S LORENSSEN ◽  
M BLENNERHASETT ◽  
W PATERSON
Keyword(s):  
Smooth Muscle

Role of stat6 in hypercontractility of murine small intestinal smooth muscle induced by nematode infection

2001 ◽  
Vol 120 (5) ◽  
pp. A534-A534
Author(s):  
A ZHAO ◽  
D MULLOY ◽  
J URBANJR ◽  
W GAUSE ◽  
T SHEADONOHUE
Keyword(s):  
Smooth Muscle ◽  
Nematode Infection ◽  
Intestinal Smooth Muscle ◽  
Small Intestinal
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