276 Does TRP channel play a role of extracellular calcium sensing in urethral smooth muscle?

2016 ◽  
Vol 15 (3) ◽  
pp. e276
Author(s):  
S. Kajioka ◽  
M. Hayashi ◽  
T. Maki ◽  
R. Takahashi ◽  
M. Etoh
Keyword(s):  
Smooth Muscle ◽  
Extracellular Calcium ◽  
Trp Channel ◽  
Calcium Sensing ◽  
Urethral Smooth Muscle

Expression of extracellular calcium-sensing receptor in human osteoblastic MG-63 cell line

2001 ◽  
Vol 280 (2) ◽  
pp. C382-C393 ◽  
Author(s):  
Toru Yamaguchi ◽  
Naibedya Chattopadhyay ◽  
Olga Kifor ◽  
Chianping Ye ◽  
Peter M. Vassilev ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Northern Blot Analysis ◽  
Pcr Primers ◽  
Extracellular Calcium ◽  
Calcium Sensing Receptor ◽  
Patch Clamp Technique ◽  
Calcium Sensing ◽  
Specific Affinity

We have previously shown the expression of the extracellular calcium (Cao 2+)-sensing receptor (CaR) in osteoblast-like cell lines, and others have documented its expression in sections of murine, bovine, and rat bone. The existence of the CaR in osteoblasts remains controversial, however, since some studies have failed to document its expression in the same osteoblast-like cell lines. The goals of the present study were twofold. 1) We sought to determine whether the CaR is expressed in the human osteoblast-like cell line, MG-63, which has recently been reported by others not to express this receptor. 2) We investigated whether the CaR, if present in MG-63 cells, is functionally active, since most previous studies have not proven the role of the CaR in mediating known actions of Cao 2+ on osteoblast-like cells. We used immunocytochemistry and Western blotting with the specific, affinity-purified anti-CaR antiserum 4637 as well as Northern blot analysis and RT-PCR using a riboprobe and PCR primers specific for the human CaR, respectively, to show readily detectable CaR protein and mRNA expression in MG-63 cells. Finally, we employed the patch-clamp technique to show that an elevation in Cao 2+ as well as the specific, allosteric CaR activator NPS R-467 (0.5 μM), but not its less active stereoisomer NPS S-467 (0.5 μM), activate an outward K+ channel in MG-63 cells, strongly suggesting that the CaR in MG-63 cells is not only expressed but is functionally active.

Extracellular calcium sensing in rat aortic vascular smooth muscle cells

2006 ◽  
Vol 348 (4) ◽  
pp. 1215-1223 ◽  
Author(s):  
Sanela Smajilovic ◽  
Jakob Lerche Hansen ◽  
Tue E.H. Christoffersen ◽  
Ewa Lewin ◽  
Søren P. Sheikh ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Extracellular Calcium ◽  
Calcium Sensing

scholarly journals Different role of muscarinic acetylcholine receptors (mAChR) in the urethral smooth muscle of male rabbit

1997 ◽  
Vol 73 ◽  
pp. 281
Author(s):  
Katsushi Nagahama ◽  
Toshihiko Tsujii ◽  
Hiroshi Azuma ◽  
Takashi Morita ◽  
Hiroyuki Oshima
Keyword(s):  
Smooth Muscle ◽  
Acetylcholine Receptors ◽  
Muscarinic Acetylcholine Receptors ◽  
Male Rabbit ◽  
Muscarinic Acetylcholine ◽  
Urethral Smooth Muscle

The Functional Role of β-Adrenoceptor Subtypes in Mediating Relaxation of Pig Urethral Smooth Muscle

2003 ◽  
Vol 170 (6) ◽  
pp. 2508-2511 ◽  
Author(s):  
TOMONORI YAMANISHI ◽  
CHRISTOPHER R. CHAPPLE ◽  
KOSAKU YASUDA ◽  
KEN-ICHIRO YOSHIDA ◽  
RUSSELL CHESS-WILLIAMS
Keyword(s):  
Smooth Muscle ◽  
Functional Role ◽  
Urethral Smooth Muscle

Role of intracellular and extracellular calcium in activation of serotonin-induced contraction of pulmonary arterial smooth muscle

1988 ◽  
Vol 106 (3) ◽  
pp. 1211-1214
Author(s):  
V. A. Buryi ◽  
A. V. Gurkovskaya ◽  
N. I. Gokina ◽  
M. F. Shuba
Keyword(s):  
Smooth Muscle ◽  
Extracellular Calcium ◽  
Arterial Smooth Muscle ◽  
Pulmonary Arterial ◽  
Pulmonary Arterial Smooth Muscle

Contribution of Cav1.2 Ca2+ channels and store-operated Ca2+ entry to pig urethral smooth muscle contraction

2020 ◽  
Vol 318 (2) ◽  
pp. F496-F505
Author(s):  
Benjamin E. Rembetski ◽  
Kenton M. Sanders ◽  
Bernard T. Drumm
Keyword(s):  
Smooth Muscle ◽  
Electrical Field Stimulation ◽  
Smooth Muscle Contraction ◽  
Isometric Tension ◽  
Electrical Field ◽  
Voltage Dependent ◽  
Urethral Smooth Muscle ◽  
Evoked Contractions ◽  
Ca2 Channels

Urethral smooth muscle (USM) generates tone to prevent urine leakage from the bladder during filling. USM tone has been thought to be a voltage-dependent process, relying on Ca2+ influx via voltage-dependent Ca2+ channels in USM cells, modulated by the activation of Ca2+-activated Cl− channels encoded by Ano1. However, recent findings in the mouse have suggested that USM tone is voltage independent, relying on Ca2+ influx through Orai channels via store-operated Ca2+ entry (SOCE). We explored if this pathway also occurred in the pig using isometric tension recordings of USM tone. Pig USM strips generated myogenic tone, which was nearly abolished by the Cav1.2 channel antagonist nifedipine and the ATP-dependent K+ channel agonist pinacidil. Pig USM tone was reduced by the Orai channel blocker GSK-7975A. Electrical field stimulation (EFS) led to phentolamine-sensitive contractions of USM strips. Contractions of pig USM were also induced by phenylephrine. Phenylephrine-evoked and EFS-evoked contractions of pig USM were reduced by ~50–75% by nifedipine and ~30% by GSK-7975A. Inhibition of Ano1 channels had no effect on tone or EFS-evoked contractions of pig USM. In conclusion, unlike the mouse, pig USM exhibited voltage-dependent tone and agonist/EFS-evoked contractions. Whereas SOCE plays a role in generating tone and agonist/neural-evoked contractions in both species, this dominates in the mouse. Tone and agonist/EFS-evoked contractions of pig USM are the result of Ca2+ influx primarily through Cav1.2 channels, and no evidence was found supporting a role of Ano1 channels in modulating these mechanisms.

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