scholarly journals Susceptibility of Plutella xylostella (Lepidoptera: Plutellidae; Linnaeus 1758) to Beauveria bassiana Bb9205, Metarhizium anisopliae Ma9236 and Heterorhabditis bacteriophora HNI0100

2014 ◽  
Vol 19 (3) ◽  
Author(s):  
Jennifer Paola Correa ◽  
M.X. Rodríguez-Bocanegra ◽  
A. Sáenz-Aponte
Keyword(s):  
Beauveria Bassiana ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Heterorhabditis Bacteriophora

scholarly journals On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Shehzad ◽  
Muhammad Tariq ◽  
Tariq Mukhtar ◽  
Asim Gulzar
Keyword(s):  
Sex Ratio ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Insect Pest ◽  
Diamondback Moth ◽  
Economic Damage ◽  
Offspring Sex Ratio ◽  
Development Of Resistance

Abstract Background The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a noxious pest of cruciferous crops all over the world causing serious economic damage. Management of insect pest generally depends on chemical control; however, due to development of resistance against all types of insecticides, alternative approaches especially utilization of a microbial agent is inevitable. Results Potential of 2 entomopathogenic fungi (EPF), viz., Beauveria bassiana and Metarhizium anisopliae, was evaluated against 2nd and 3rd larval instars of P. xylostella by adopting leaf dip and direct spraying methods under laboratory conditions. Significant mortality rate was achieved by each fungus under adopted methodologies. However, B. bassiana was found to be more effective in both conditions than M. anisopliae. Highest mean corrected mortality (77.80%) was recorded, when spores of B. bassiana were sprayed on the 2nd instar larvae (LC50=1.78×104/ml) after the 6th day of treatment. Similarly, incase of M. anisopliae LC50 for the 2nd instar at the same methodology was 2.78×104/ml with a mortality percentage of 70.0%. Offspring sex ratio was non-significantly related to treatment concentration and methodology, except for the control. Conclusion Beauveria bassiana and M. anisopliae had potential to suppress P. xylostella infestations when applied appropriately. Present findings suggested that B. bassiana and M. anisopliae when sprayed on immatures of host insect had more effect as compared to leaf dip procedure. Furthermore, no significant effect of concentrations was observed on sex ratio.

scholarly journals UJI EFIKASI BIOINSEKTISIDA JAMUR ENTOMOPATOGEN BERFORMULASI CAIR TERHADAP Plutella xylostella (L.) DI LABORATORIUM

2013 ◽  
Vol 13 (1) ◽  
pp. 52-60
Author(s):  
Haperidah Nunilahwati ◽  
Siti Herlinda ◽  
Chandra Irsan ◽  
Yulia Pujiastuti ◽  
Khodijah Khodijah ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Active Ingredient ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Crop Production ◽  
Insect Pest ◽  
Larval Mortality ◽  
Third Instar Larvae

Efficacy test of liquid bio-insecticide of entomopathogenic fungi in control against Plutella xylostella in the laboratory.  The insect pest P. xylostella could reduce crop production of Brassicaceae. The aim of research was to test the efficacy liquid bio insecticide with active ingredient of Beauveria bassiana and Metarhizium anisopliae fungi to control P. xylostella. Bio-insecticide was applied by spraying  on mustard leaves infested with 50 individuals of third instar larvae of P. xylostella and a density of 1x106 conidia ml-1. Larval mortality was observed every 2 hours and LT50 of larvae was calculated. The study showed that the highest percentage of mortality found in Mt ES and Mt ES (cf) isolates was 99.6%, the lowest mortality at Mt NES isolate was 96.80%. LT50 and LT95 values   Bb ES were the lowest i.e. 2.04 days and 2.95 days. The highest LT50 and LT95 of Mt NES isolate were 2.24 days and 3.32 days. The liquid bio-insecticide of entomopathogenic fungus B. bassiana and M. anisopliae were effective to control the larvae of P. xylostella.

Potential of Beauveria bassiana and Metarhizium anisopliae isolates from Benin to control Plutella xylostella L. (Lepidoptera: Plutellidae)

2009 ◽  
Vol 28 (3) ◽  
pp. 220-224 ◽  
Author(s):  
I. Godonou ◽  
B. James ◽  
C. Atcha-Ahowé ◽  
S. Vodouhè ◽  
C. Kooyman ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae

scholarly journals Effect of endophytic isolates of Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metchnikoff) Sorokin on Plutella xylostella (L.) (Lepidoptera: Plutellidae) in cabbage

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Poornesha Bathina ◽  
Ramanujam Bonam
Keyword(s):  
Beauveria Bassiana ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Seed Treatment ◽  
Foliar Application ◽  
Diamondback Moth ◽  
Main Body ◽  
Inoculation Methods ◽  
Application Method ◽  
Application Methods

Abstract Background The diamondback moth (Plutella xylostella L.) (Lepidoptera: Plutellidae) is one of the major pests in cabbage which causes severe loss to the cruciferous crops. Entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae have been established as endophytes in cabbage plants by seed treatment/root inoculation/foliar application methods in glasshouse conditions. Main body A glasshouse experiment was conducted to study the effect of endophytic isolates of B. bassiana (NBAIR Bb-5a and NBAIR Bb-45) and M. anisopliae (NBAIR Ma-4 and NBAIR Ma-35) on P. xylostella in cabbage using detached leaf bioassay method. The isolates were applied through seed treatment/root inoculation/foliar application at the concentration of 1 × 108 spores/ml and evaluated at 15, 30, 45, and 60 days after treatment (DAT) in 2017 and 2018. These isolates were colonized in cabbage leaf tissues from 15 to 60 DAT. All 4 isolates showed different mortality percentages during 15–30 DAT, and no mortality was observed after 45 and 60 DAT in different inoculation methods during both years. Among the application methods tested, foliar application method gave the highest mortality of 70–80% at 15 DAT and 12–58% at 30 DAT mortality of 2nd instar larvae of P. xylostella in cabbage. Among the isolates tested, NBAIR Ma-35 showed the highest mortality (35–79%) in all the 3 inoculation methods tested. Conclusion Endophytic isolates of B. bassiana and M. anisopliae suppressed the population of P. xylostella on cabbage leaves in three inoculation methods tested. Among all the methods tested, foliar application method showed highest mortality. These promising isolates have to be further tested under field conditions for management of P. xylostella in cabbage.

scholarly journals Control Biológico del Salivazo de la Caña de Azúcar Aeneolamia sp. con el Nematodo Heterorhabditis bacteriophora y los Hongos Entomopatógenos Metarhizium anisopliae y Beauveria bassiana como Opción Económica y Sostenible

Ceiba ◽  
2018 ◽  
Vol 55 (1) ◽  
pp. 21-27
Author(s):  
José R. Pérez Milián ◽  
Yosel Pérez Pérez ◽  
Jorge F. Álvarez ◽  
Jorge Mario Ruano Rossil
Keyword(s):  
Beauveria Bassiana ◽  
Metarhizium Anisopliae ◽  
Heterorhabditis Bacteriophora

El nematodo entomopatógeno Heterorhabditis bacteriophora (Poinar) (Rhabditida: Heterorhabditidae) fue evaluado como opción potencial de control biológico del salivazo de la caña de azúcar, Aeneolamia spp. (Hemiptera: Cercopidae). Los estudios de campo muestran un control biológico efectivo utilizando este nematodo sobre los estadios ninfales del salivazo y cuyo efecto se hace evidente a pocas horas de haber sido aplicado. Hubo mayor mortalidad en los estadios ninfales 4 y 5 del salivazo y la toxicidad del nematodo se prolongó por semanas después de su aplicación en el campo. En algunos ensayos se observó superioridad estadística de H. bacteriophora sobre los hongos entomopatógenos Metarhizium anisopliae (Hypocreales: Clavicipitaceae) y Beauveria bassiana (Hypocreales: Cordycipitaceae) (Prueba de Tukey al 0.05 y 0.01 niveles de significancia), así como frente a otros agentes de control biológico alternativos. 

Evaluación de hongos entomopatógenos para el control biológico del mosquito Culex quinquefasciatus

2009 ◽  
Vol 5 (9) ◽  
pp. 25-38
Author(s):  
Martha Giselle Rivera Pineda ◽  
Sandra Patricia Garzón Lozano ◽  
Luz Inés Villarreal Salazar
Keyword(s):  
Beauveria Bassiana ◽  
Culex Quinquefasciatus ◽  
Metarhizium Anisopliae

En busca de una alternativa de control del mosquito Culex quinquefasciatus en áreas urbanas se desarrolló el proyecto: “Evaluación de microorganismos biocontroladores sobre el mosquito Culex quinquefasciatus” cuyo objetivo fue evaluar la capacidad biocontroladora de diferentes hongos entomopatógenos sobre larvas del mosquito a nivel de laboratorio. Se evaluaron 16 aislamientos de hongos en diferentes concentraciones. Se constataron diferencias en el porcentaje de mortalidad producido en larvas de Cx. quinquefasciatus dependientes del tipo de aislamiento, de la concentración utilizada y del tiempo de exposición. Los aislamientos que presentaron el nivel de mortalidad más alto pertenecen a la especie Metarhizium anisopliae, seguidos por aislamientos de la especie Beauveria bassiana. El aislamiento que presentó el mayor nivel de mortalidad (100%) en menor tiempo de exposición (24 horas) fue Metarhizium anisopliae 99068 en una concentración de 1x106 e/ml. Por lo tanto este aislamiento es recomendado para su evaluación en campo y desarrollo de un producto comercial.

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