scholarly journals Effect of endophytic isolates of Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metchnikoff) Sorokin on Plutella xylostella (L.) (Lepidoptera: Plutellidae) in cabbage

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Poornesha Bathina ◽  
Ramanujam Bonam
Keyword(s):  
Beauveria Bassiana ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Seed Treatment ◽  
Foliar Application ◽  
Diamondback Moth ◽  
Main Body ◽  
Inoculation Methods ◽  
Application Method ◽  
Application Methods

Abstract Background The diamondback moth (Plutella xylostella L.) (Lepidoptera: Plutellidae) is one of the major pests in cabbage which causes severe loss to the cruciferous crops. Entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae have been established as endophytes in cabbage plants by seed treatment/root inoculation/foliar application methods in glasshouse conditions. Main body A glasshouse experiment was conducted to study the effect of endophytic isolates of B. bassiana (NBAIR Bb-5a and NBAIR Bb-45) and M. anisopliae (NBAIR Ma-4 and NBAIR Ma-35) on P. xylostella in cabbage using detached leaf bioassay method. The isolates were applied through seed treatment/root inoculation/foliar application at the concentration of 1 × 108 spores/ml and evaluated at 15, 30, 45, and 60 days after treatment (DAT) in 2017 and 2018. These isolates were colonized in cabbage leaf tissues from 15 to 60 DAT. All 4 isolates showed different mortality percentages during 15–30 DAT, and no mortality was observed after 45 and 60 DAT in different inoculation methods during both years. Among the application methods tested, foliar application method gave the highest mortality of 70–80% at 15 DAT and 12–58% at 30 DAT mortality of 2nd instar larvae of P. xylostella in cabbage. Among the isolates tested, NBAIR Ma-35 showed the highest mortality (35–79%) in all the 3 inoculation methods tested. Conclusion Endophytic isolates of B. bassiana and M. anisopliae suppressed the population of P. xylostella on cabbage leaves in three inoculation methods tested. Among all the methods tested, foliar application method showed highest mortality. These promising isolates have to be further tested under field conditions for management of P. xylostella in cabbage.

scholarly journals On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Shehzad ◽  
Muhammad Tariq ◽  
Tariq Mukhtar ◽  
Asim Gulzar
Keyword(s):  
Sex Ratio ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Insect Pest ◽  
Diamondback Moth ◽  
Economic Damage ◽  
Offspring Sex Ratio ◽  
Development Of Resistance

Abstract Background The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a noxious pest of cruciferous crops all over the world causing serious economic damage. Management of insect pest generally depends on chemical control; however, due to development of resistance against all types of insecticides, alternative approaches especially utilization of a microbial agent is inevitable. Results Potential of 2 entomopathogenic fungi (EPF), viz., Beauveria bassiana and Metarhizium anisopliae, was evaluated against 2nd and 3rd larval instars of P. xylostella by adopting leaf dip and direct spraying methods under laboratory conditions. Significant mortality rate was achieved by each fungus under adopted methodologies. However, B. bassiana was found to be more effective in both conditions than M. anisopliae. Highest mean corrected mortality (77.80%) was recorded, when spores of B. bassiana were sprayed on the 2nd instar larvae (LC50=1.78×104/ml) after the 6th day of treatment. Similarly, incase of M. anisopliae LC50 for the 2nd instar at the same methodology was 2.78×104/ml with a mortality percentage of 70.0%. Offspring sex ratio was non-significantly related to treatment concentration and methodology, except for the control. Conclusion Beauveria bassiana and M. anisopliae had potential to suppress P. xylostella infestations when applied appropriately. Present findings suggested that B. bassiana and M. anisopliae when sprayed on immatures of host insect had more effect as compared to leaf dip procedure. Furthermore, no significant effect of concentrations was observed on sex ratio.

scholarly journals Comparative efficacy of endophytic versus foliar application of the entomopathogenic fungus Beauveria bassiana against the crucifer diamondback moth larvae for sustainable cabbage protection

2020 ◽  
Vol 14 (4) ◽  
pp. 1448-1458
Author(s):  
Gabriel A. Heviefo ◽  
Seth W. Nyamador ◽  
Seth W. Nyamador ◽  
Benjamin D. Datinon ◽  
Isabelle A. Glitho ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Plutella Xylostella ◽  
Untreated Control ◽  
Molecular Mechanisms ◽  
Foliar Application ◽  
Larval Mortality ◽  
Diamondback Moth ◽  
Mortality Rates ◽  
Comparative Efficacy ◽  
Larval Instars

In recent investigations, Beauveria bassiana has been reported to be pathogenic to insect, endophytically colonize cabbage plants and may, therefore, be an alternative to chemical control of the diamondback moth, Plutella xylostella. In this study, the comparative efficacy of endophytic and foliar application of B. bassiana was assessed on different larval instars of P. xylostella. Cabbage plants were inoculated with B. bassiana conidia using seed coating and root soaking methods. Six weeks after plant endophytic inoculation, larval instars 2, 3 and 4 of P. xylostella were released on endophytic plant leaves without fungal spraying, on non-endophytic leaves sprayed immediately with fungal formulation and on untreated control leaves. Our results indicate that the average mortality rates of larval instars 2, 3 and 4 were significantly higher (P < 0.0001) on plants colonized by the endophyte than non-inoculated, untreated control plant. Similarly, significant differences were obtained between the foliar spraying of the fungus (corrected mortalities ≥ 96.72%) and the endophytic applications (corrected mortalities ≤ 57.61 %). In endophytic treatments, larval mortality rates varied with larval age. Further investigations are needed to elucidate the cellular and molecular mechanisms of B. bassiana endophytism in cabbage.Keywords: Fungal endophyte, foliar application, cabbage plant, Plutella xylostella, larval mortality, cadaver sporulation.

scholarly journals UJI EFIKASI BIOINSEKTISIDA JAMUR ENTOMOPATOGEN BERFORMULASI CAIR TERHADAP Plutella xylostella (L.) DI LABORATORIUM

2013 ◽  
Vol 13 (1) ◽  
pp. 52-60
Author(s):  
Haperidah Nunilahwati ◽  
Siti Herlinda ◽  
Chandra Irsan ◽  
Yulia Pujiastuti ◽  
Khodijah Khodijah ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Active Ingredient ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Crop Production ◽  
Insect Pest ◽  
Larval Mortality ◽  
Third Instar Larvae

Efficacy test of liquid bio-insecticide of entomopathogenic fungi in control against Plutella xylostella in the laboratory.  The insect pest P. xylostella could reduce crop production of Brassicaceae. The aim of research was to test the efficacy liquid bio insecticide with active ingredient of Beauveria bassiana and Metarhizium anisopliae fungi to control P. xylostella. Bio-insecticide was applied by spraying  on mustard leaves infested with 50 individuals of third instar larvae of P. xylostella and a density of 1x106 conidia ml-1. Larval mortality was observed every 2 hours and LT50 of larvae was calculated. The study showed that the highest percentage of mortality found in Mt ES and Mt ES (cf) isolates was 99.6%, the lowest mortality at Mt NES isolate was 96.80%. LT50 and LT95 values   Bb ES were the lowest i.e. 2.04 days and 2.95 days. The highest LT50 and LT95 of Mt NES isolate were 2.24 days and 3.32 days. The liquid bio-insecticide of entomopathogenic fungus B. bassiana and M. anisopliae were effective to control the larvae of P. xylostella.

Impact of Cobalt Application Methods on Chickpea Yield, Nutrient Uptake and Status of Soil

2019 ◽  
Author(s):  
Santosh Onte ◽  
Nitin N Gudadhe ◽  
Nilima Karmakar ◽  
Raju G. Ladumor
Keyword(s):  
Seed Yield ◽  
Seed Treatment ◽  
Foliar Application ◽  
Block Design ◽  
Cobalt Content ◽  
Seed Priming ◽  
Nutrient Content ◽  
Soil Application ◽  
Application Methods ◽  
The Impact

A field experiment was conducted during rabi season of 2015-2016 at Navsari Agricultural University, Navsari to study the impact of cobalt application methods on chickpea yield, nutrient content and soil status. Four cobalt application methods with three levels of each were evaluated with one absolute control and one with seed priming of water were evaluated with randomized block design with three replications. Least level of seed treatment, soil application and foliar application of cobalt gave highest chickpea seed yield and decreased there after. As cobalt levels increased, NPK content and uptake are decreased linearly in all the methods except seed priming method, however cobalt content and uptake increased linearly by seed treatment and foliar application, on the contrary it decreased linearly by seed priming and soil application of cobalt. Soil application of cobalt at 50 g ha-1 recorded highest chickpea seed yield, residual NPK and Co and which can be useful for succeeding crop and this can be recommended from the cobalt nutrition point of view in plants and animals followed by seed priming at 1 ppm.

scholarly journals Ionic responses of bean (Phaseolus vulgaris L.) plants under salinity stress and humic acid applications

2020 ◽  
Vol 48 (3) ◽  
pp. 1317-1331
Author(s):  
Nurdilek GULMEZOGLU ◽  
Ezgi İZCI
Keyword(s):  
Humic Acid ◽  
Chlorophyll Content ◽  
Dry Matter ◽  
Foliar Application ◽  
Dry Weight ◽  
Soil Application ◽  
Salt Damage ◽  
Application Method ◽  
Bean Plants ◽  
Application Methods

This study aimed to investigate the effects of different humic acid application methods (control, soil, foliar and soil + foliar) on chlorophyll content, dry matter weight of shoots and roots, concentrations of potassium (K), calcium (Ca) and sodium (Na), and K/Na and Ca/Na ratios of bean plants exposed to increasing salinity levels (0, 50, 100 and 150 mM). The effects of salt damage on shoots and roots of bean plants were significantly differed in humic acid application methods. Chlorophyll content decreased with the increase in salt doses at control and soil application of humic acid, while the decrease in chlorophyll content was lower in foliar application of humic acid. Shoot dry weight was not affected by humic acid applications, while root dry weight increased in soil + foliar application method. Soil + foliar humic acid application caused the highest shoot and root Na concentrations. Shoot Ca (2.61%) concentration in soil + foliar application was significantly higher compared to the other treatments, while the highest Ca concentration in roots (1.55%) was recorded in soil humic acid application method. The highest K concentration in roots was obtained in the control treatment (2.50%) followed by soil + foliar humic acid application (2.48%). The ratios of K/Na and Ca/Na in shoots decreased with the increase in salt application rates. The highest shoot K/Na (1456.1%) and Ca/Na (1274.1%) ratio in humic acid x salt interactions was found in soil application of humic acid without salt treatment. The root and shoot dry matter yield and K and Ca concentrations of the plants indicated that soil+foliar application method has a preventive effect for the plants against the 50 mM salt damage. The results showed that soil+foliar humic acid application in addition to the mineral fertilization required for beans can contribute to the growth and mineral nutrition of the plants under moderate salt stress (50 mM NaCl).

Potential of Beauveria bassiana and Metarhizium anisopliae isolates from Benin to control Plutella xylostella L. (Lepidoptera: Plutellidae)

2009 ◽  
Vol 28 (3) ◽  
pp. 220-224 ◽  
Author(s):  
I. Godonou ◽  
B. James ◽  
C. Atcha-Ahowé ◽  
S. Vodouhè ◽  
C. Kooyman ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae

Efficacy and Persistence of Beauveria bassiana and Other Fungi for Control of Diamondback Moth (Lepidoptera: Plutellidae) on Cabbage Seedlings1

1998 ◽  
Vol 33 (2) ◽  
pp. 142-151 ◽  
Author(s):  
A. M. Shelton ◽  
J. D. Vandenberg ◽  
M. Ramos ◽  
W. T. Wilsey
Keyword(s):  
Beauveria Bassiana ◽  
Metarhizium Anisopliae ◽  
Diamondback Moth ◽  
Resistance Management ◽  
Management Program ◽  
Control Problems ◽  
Paecilomyces Fumosoroseus ◽  
Single Application ◽  
Damage Reduction ◽  
Insect Populations

The diamondback moth, Plutella xylostella (L.), is a key pest of crucifers worldwide. Resistance by diamondback moths to chemical insecticides and Bacillus thuringiensis Berliner toxins highlights the need for alternative controls. Crucifer growers often depend on seedlings produced in screenhouses and later transplanted to the field. Commercial seedlings can be contaminated with pesticide-resistant P. xylostella, leading to control problems in the field. We evaluated the efficacy of Beauveria bassiana (Balsamo) Vuillemin applied as conidia in Mycotrol ® WP in three experiments by evaluating insect control, damage reduction, and fungus persistence on treated leaves of cabbage seedlings. In one experiment, we also evaluated Metarhizium anisopliae Metschnikoff (Sorokin) applied as conidia in Bio-blast® and Paecilomyces fumosoroseus (Wize) Brown & Smith applied as freshly-cultivated unformulated blastospores. We observed significant reductions, compared to controls, in insect populations and damage ratings when Mycotrol was applied once- or twice-weekly. Mycotrol treatments were as effective as a B. thuringiensis product in preventing damage when three spray nozzles were used to insure adequate pesticide coverage. Mycotrol persisted on treated leaves in screen-houses for more than 2 wks, and mycosis of larvae reared on these leaves was &gt;50% 7 d after a single application of fungus. Treatment with either M. anisopliae or P. fumosoroseus resulted in no significant reduction in insect numbers or damage, but each fungus persisted on leaves and caused mycosis in lab-reared larvae more than 2 wks after treatment. Mycotrol can provide an option for control of P. xylostella larvae on seedlings, and may be especially useful in a resistance management program.

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