scholarly journals UJI EFIKASI BIOINSEKTISIDA JAMUR ENTOMOPATOGEN BERFORMULASI CAIR TERHADAP Plutella xylostella (L.) DI LABORATORIUM

2013 ◽  
Vol 13 (1) ◽  
pp. 52-60
Author(s):  
Haperidah Nunilahwati ◽  
Siti Herlinda ◽  
Chandra Irsan ◽  
Yulia Pujiastuti ◽  
Khodijah Khodijah ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Active Ingredient ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Crop Production ◽  
Insect Pest ◽  
Larval Mortality ◽  
Third Instar Larvae

Efficacy test of liquid bio-insecticide of entomopathogenic fungi in control against Plutella xylostella in the laboratory.  The insect pest P. xylostella could reduce crop production of Brassicaceae. The aim of research was to test the efficacy liquid bio insecticide with active ingredient of Beauveria bassiana and Metarhizium anisopliae fungi to control P. xylostella. Bio-insecticide was applied by spraying  on mustard leaves infested with 50 individuals of third instar larvae of P. xylostella and a density of 1x106 conidia ml-1. Larval mortality was observed every 2 hours and LT50 of larvae was calculated. The study showed that the highest percentage of mortality found in Mt ES and Mt ES (cf) isolates was 99.6%, the lowest mortality at Mt NES isolate was 96.80%. LT50 and LT95 values   Bb ES were the lowest i.e. 2.04 days and 2.95 days. The highest LT50 and LT95 of Mt NES isolate were 2.24 days and 3.32 days. The liquid bio-insecticide of entomopathogenic fungus B. bassiana and M. anisopliae were effective to control the larvae of P. xylostella.

scholarly journals On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Shehzad ◽  
Muhammad Tariq ◽  
Tariq Mukhtar ◽  
Asim Gulzar
Keyword(s):  
Sex Ratio ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Insect Pest ◽  
Diamondback Moth ◽  
Economic Damage ◽  
Offspring Sex Ratio ◽  
Development Of Resistance

Abstract Background The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a noxious pest of cruciferous crops all over the world causing serious economic damage. Management of insect pest generally depends on chemical control; however, due to development of resistance against all types of insecticides, alternative approaches especially utilization of a microbial agent is inevitable. Results Potential of 2 entomopathogenic fungi (EPF), viz., Beauveria bassiana and Metarhizium anisopliae, was evaluated against 2nd and 3rd larval instars of P. xylostella by adopting leaf dip and direct spraying methods under laboratory conditions. Significant mortality rate was achieved by each fungus under adopted methodologies. However, B. bassiana was found to be more effective in both conditions than M. anisopliae. Highest mean corrected mortality (77.80%) was recorded, when spores of B. bassiana were sprayed on the 2nd instar larvae (LC50=1.78×104/ml) after the 6th day of treatment. Similarly, incase of M. anisopliae LC50 for the 2nd instar at the same methodology was 2.78×104/ml with a mortality percentage of 70.0%. Offspring sex ratio was non-significantly related to treatment concentration and methodology, except for the control. Conclusion Beauveria bassiana and M. anisopliae had potential to suppress P. xylostella infestations when applied appropriately. Present findings suggested that B. bassiana and M. anisopliae when sprayed on immatures of host insect had more effect as compared to leaf dip procedure. Furthermore, no significant effect of concentrations was observed on sex ratio.

scholarly journals Effectiveness of Selected Entomopathogenic Fungi in Packed Rice Grain at Room Temperature Against Corcyra Cephalonica Stainton

2017 ◽  
Vol 23 (3) ◽  
pp. 183 ◽  
Author(s):  
Hendrawan Samodra ◽  
Yusof Bin Ibrahim
Keyword(s):  
Beauveria Bassiana ◽  
High Mortality ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Room Temperature ◽  
Larval Mortality ◽  
Corcyra Cephalonica ◽  
Rice Grain ◽  
Rice Moth ◽  
Tapioca Flour

Eight isolates of entomopathogenic fungi were evaluated as dried conidia against the rice moth,  Corcyra cephalonica. In bioassays two isolates of Beauveria bassiana (BbGc and BbPs) and one isolate of Metarhizium anisopliae (MaPs) consistently gave high mortality to C. cephalonica larvae. Formulations in either kaolin, talc or tapioca flour (20 % w/w a.i.) thoroughly mixed with long grain rice in plastic cups (8 cm diameter by 5 cm) gave complete larval mortality by the 12th day of treatment. However, in general those formulated in kaolin and talc were more efficacious and faster to kill compared to those formulated in tapioca flour or the unformulated control. Even at the lowest rate of 0.05 g BbGc in kaolin provided 100% mortality 7 days after introduction compared with other dust formulations. Isolate BbGc in kaolin and talc administered at 0.4 g a.i. in 200 g rice packed in plastic kept at room temperature provided protection against the rice moth up to 4 months of storage. Larval mortality in excess of 90% was obtained 15 days after introduction. Formulations of MaPs was effective only within the first month of storage beyond which infectivity rapidly declined.

scholarly journals Entomopathogenic fungi isolated in the vicinity of Szczecin

2014 ◽  
Vol 33 (1) ◽  
pp. 123-130 ◽  
Author(s):  
Ryszard Miętkiewicz ◽  
Magdalena Dzięgielewska ◽  
Krystyna Janowicz
Keyword(s):  
Forest Soil ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Dominant Species ◽  
Beech Forest ◽  
Meadow Soil ◽  
Paecilomyces Farinosus ◽  
Opposite Tendency

The spectrum and occurrence of entomopathogenic fungi in litter beech forest. meadow soil and arable soil were evaluated using an "insect bait method". Soil and litter samples taken in autumn and spring from each stand were baited with <i>G. mellonella</i> larvae at 22°C. Six species of entomopathogenic fungi infected these larvae. In the litter <i>Beauveria bassiana</i> was the dominant species. Besides <i>B. bassiana</i>, 23% of <i>G. mellonella</i> larvae were infected by <i>Paecilomyces farinosus</i> in autumn. In the forest soil the dominant entomopathogenic fungus was not found. In this stand the number of infected larvae was the lowest. <i>Metarhizium anisopliae</i> was the dominant species in the meadow soil in both sampling periods. In the soil taken from a rye field<i> P. fumosoroseus</i> and <i>B. bassiana</i> infected <i>G. mellonella</i> larvae almost to the same degree. It was established that <i>B. bassiana</i> and <i>M. anisopliae</i> infected more <i>G. mellonella</i> larvae in spring than in autumn. In the case of <i>P. farinosus</i> and <i>P. fumosoroseus</i> an opposite tendency was observed.

scholarly journals Patogenisitas Cendawan Metarhizium anisopliae (Metsch) dalam mengendalikan Kepik Hijau (Nezara viridula L.) pada Stadia Perkembangan yang Berbeda di Laboratorium

2020 ◽  
Vol 4 (2) ◽  
pp. 178-187
Author(s):  
Ahmad Alwi Azhari ◽  
Muhammad Sayuthi ◽  
Hasnah Hasnah
Keyword(s):  
Incubation Period ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Biological Control Agent ◽  
Insect Pest ◽  
Instar Nymph ◽  
Nezara Viridula ◽  
Stink Bug ◽  
Death Time

Abstrak. Nezara viridula (L.) (Hemiptera: Pentatomidae) adalah serangga hama yang bersifat kosmopolit dan mempunyai banyak tanaman inang (polifagus) antara lain kacang-kacangan dan kubis-kubisan. Serangan serangan hama ini dapat menurunkan kualitas dan kuantitas hasil tanaman. Salah satu metoda pengendalian hama yang ramah lingkungan yaitu penggunaan cendawan entomopatogen Metarhizium anisopliae sebagai agen pengendalian hayati yang dapat menyebabkan penyakit green muscardin fungus pada serangga. Keefektifan cendawan entomopatogen sangat dipengaruhi oleh stadia perkembangan inang dan kerapatan konidia cendawan yang diaplikasikan. Tujuan dari penelitian ini adalah untuk mendapatkan konsentrasi yang efektif dari cendawan M. anisopliae dan stadia perkembangan serangga yang paling rentan terhadap cendawan M. anisopliae. Rancangan yang digunakan dalam penelitian adalah Rancangan Acak Lengkap faktorial yang terdiri dari 2 faktor yaitu konsentrasi cendawan M. anisopliae: K1 (4g/100 ml), K2 (6g/100 ml) dan K3 (8g/100 ml) serta stadia perkembangan serangga: N1 (Nimfa instar 2), N2 (Nimfa instar 4) dan N3 (Imago). Menghasilkan jumlah kombinasi perlakuan 9 dengan 3 ulangan sehingga terdapat 27 unit percobaan. Hasil penelitian menunjukkan bahwa aplikasi cendawan entomopatogen M. anisopliae berpengaruh nyata terhadap masa inkubasi, mortalitas, dan waktu kematian dari N. viridula. Masa inkubasi tercepat pada 8g/ 100 ml yaitu 1,33 hari dan terlama pada 4g/ 100 ml yaitu 1,44 hari. Masa inkubasi tercepat pada nimfa instar 2 yaitu 1,00 hari dan terlama pada imago yaitu 2,11 hari. Mortalitas tertinggi yaitu pada 8g/ 100 ml sebesar 97,67% dan terendah pada 4g/ 100 ml sebesar 87,78% pengamatan 8 HSA. Mortalitas tertinggi terjadi pada nimfa instar 2 sebesar 98,89% dan terendah pada imago sebesar 86,67% pengamatan 8 HSA. Waktu kematian tercepat pada perlakuan 8g/ 100 ml sebesar 4,49 hari dan terendah pada perlakuan 4g/ 100 ml sebesar 5,50 hari. Waktu kematian tercepat pada nimfa instar 2 sebesar 4,31 hari dan terendah pada imago sebesar 6,00 hari. Cendawan entomopatogen M. anisopliae efektif dalam mengendalikan N. viridula pada nimfa instar 2 dengan konsentrasi 8g/ 100 ml.Pathogenicity of Fungi Metarhizium anisopliae (Metsch) in controlling Green Stink bug (Nezara viridula L.) at Different Developmental Stages in LaboratoryAbstract. Nezara viridula (L.) (Hemiptera: Pentatomidae) is a cosmopolitan insect pest and has many host plants (polyphagus) such as beans and cabbage. Attack of the pests can reduce the quality and quantity of crops. One of the most environmentally friendly methods of pest control is the use of entomopathogenic fungus Metarhizium anisopliae as a biological control agent can cause green muscard fungus disease in insects. The effectiveness of entomopathogenic fungi is strongly influenced by the stadia of host development and the density of conidial fungi applied. The aim of this study is to obtain an effective concentration of the fungus M. anisopliae and the stages of development of the most vulnerable insects against the fungus M. anisopliae. The design used in the study was a Factorial Randomized Complete Random consisting of two factors: the concentration of fungus M. anisopliae: K1 (4g / 100 ml), K2 (6g / 100 ml) and K3 (8g / 100 ml) and stages insect development: N1 (instar nymph 2), N2 (instar nymph 4) and N3 (Adult). Produced a combined number of treatments 9 with 3 replications so that there were 27 experimental units. The result showed that the application of entomopathogenic fungi M. anisopliae had significant effect on the incubation period, mortality, and death time of N. viridula. The fastest incubation period at 8g / 100ml is 1.33 days and the longest at 4g / 100ml is 1.44 days. The fastest incubation period in nymph instar 2 is 1.00 days and the longest in adult is 2.11 days. The highest mortality is at 8g / 100 ml of 97,67% and the lowest at 4g / 100 ml equal to 87,78% observation 8 HSA. The highest mortality occurred in instar nymph 2 of 98.89% and the lowest in adult of 86,67% observation 8 HSA. The fastest death time in the 8g / 100 ml treatment was 4.49 days and the lowest in the 4g / 100 ml treatment was 5.50 days. The fastest death time in instar nymph 2 is 4.31 days and the lowest is adult at 6.00 days. The entomopathogenic fungus M. anisopliae is effective in controlling N. viridula in instar nymph 2 with a concentration of 8g / 100 ml.

scholarly journals Eksplorasi, isolasi dan identifikasi Jamur entomopatogen yang menginfeksi serangga hama

2020 ◽  
Vol 1 (2) ◽  
pp. 70
Author(s):  
Arsi Arsi ◽  
Yulia Pujiastuti ◽  
Suparman Surya Hadi Kusuma ◽  
Bambang Gunawan
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Entomopathogenic Fungus ◽  
Insect Pests ◽  
Insect Pest ◽  
Entire Body ◽  
Vegetable Crops

ABSTRACT Entomopathogenic fungi are classified as a fungus that infects insect pests. Hyphae color that comes out of the insect's body depends on the type of entomopathogenic fungus that attacks it. The purpose of this research is to explore, isolate and identify entomopathogenic fungi that attack insect pests. Samples of dead insects were taken from vegetable crops in the highlands of Pagaralam City and the lowlands of Ogan Komering Ilir Regency. In addition, exploration is also carried out using insect bait methods. This method is carried out on soils taken from the highlands of the fencealam area and Pekanbaru area. Based on the results of entomopathogenic expolation in vegetable plants that infect insects in the field there are 2 types of fungi that attack the insect. Two types of entomapatogenic fungi were found to have characteristics, namely the first hyphal color is rather green which covers the entire body of the insect and the second is white hyphae. The fungus that attacks the insect, then isolated to the GYA media found two entomopathogenic fungi namely, Metarhizium sp. and Beauveria bassiana. Meanwhile, through insect bait using soil T. molitor larvae, one species of entomopathogenic fungus was found, namely Metarhizium sp. The most infected T. molitor larvae in the soil of origin of Pekanbaru in the 2nd and 4th weeks, namely, 21.90 and 29.33 tails.   . Keywords: Entomopathogenic Fungus, Insect Pest and Tenibrio molitor  

scholarly journals KERAPATAN DAN VIABILITAS KONIDIA BEAUVERIA BASSIANA DAN METARHIZIUM ANISOPLIAE PADA MEDIA IN VITRO PH RENDAH

2017 ◽  
Vol 17 (2) ◽  
pp. 119
Author(s):  
Lilian Rizkie ◽  
Siti Herlinda ◽  
Suwandi . ◽  
Chandra Irsan ◽  
Susilawati . ◽  
...  
Keyword(s):  
Shelf Life ◽  
Beauveria Bassiana ◽  
Active Ingredient ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Low Ph ◽  
Conidial Viability

Conidial density and viability of Beauveria bassiana and Metarhizium anisopliae grown on the low-pH in vitro medium. Liquid bioinsecticide with active ingredient from conidial entomopathogenic fungus has major constraints, namely short shelf life due to declining conidial viability and density is caused by low pH in the bioinsecticide carrier. This experiment aimed to measure the loss of conidial viability and density of Beauveria bassiana and Metarhizium anisopliae isolates grown on in vitro medium with low pH. Entomopathogenic fungus isolates were used as much as 28 isolates grown on in vitro medium at low pH, namely pH 5, 4, 3, and 2. The results showed that the fungus isolate that had the highest conidial density on in vitro medium at pH 5 was found on isolates of B. bassiana with code BPcMs (2.583 x 109 conidia mL-1), while the lowest one was found on isolates of B. bassiana with code of BWS Pantura (0.825x109 conidia mL-1). All isolate conidial density from in vitro medium with pH 2 decreased regularly. Conidial density of BPcMs isolate decreased to 2.483 x 109conidia mL-1, as well as BWS Pantura isolate also decreased to 0.425x109 conidia mL-1. The highest conidial viability at pH 5 was found on isolates of B. bassiana with code of BPcMs (51.572%), while the lowest conidial viability was found on isolate of B. bassiana with BTmPc code (15.040%). At pH 2, almost isolates tested had low conidial viability. The conidial viability of isolates BPcMs decreased to 47.037%%, while the isolates BTmPc also decreased to 12.778%. Therefore, the lower of the pH of the in vitro medium was, the lower of conidial viability and density of B. bassiana and M. anisopliae was

scholarly journals EKSPLORASI, ISOLASI DAN SELEKSI JAMUR ENTOMOPATOGEN PLUTELLA XYLOSTELLA (LEPIDOPTERA: YPONOMEUTIDAE) PADA PERTANAMAN CAISIN (BRASSICA CHINENSIS) DI SUMATERA SELATAN

2012 ◽  
Vol 12 (1) ◽  
pp. 1-11
Author(s):  
Haperidah Nunilahwati ◽  
Siti Herlinda ◽  
Chandra Irsan ◽  
Yulia Pujiastuti
Keyword(s):  
Entomopathogenic Fungi ◽  
Plutella Xylostella ◽  
Entomopathogenic Fungus ◽  
Insect Pests ◽  
Biological Agents ◽  
Infected Host ◽  
Brassica Chinensis ◽  
Fungal Isolates ◽  
Test Insect ◽  
Third Instar Larvae

Plutella xylostella is the most destructive insect pests of the brassicae family. The research objective was to explore, isolate and select entomopathogenic fungi as biological agents for control of P. xylostella. This study used 20 fungal isolates originating from soil and infected insects around the farmers’ field in lowland and highland of South Sumatra. The fungal isolates were tested to third instar larvae of P.xylostella. The suspension of entomopathogenic fungus was topical inoculated with a density of 1x106 conidia ml-1 on the test insect and five replicates. The result showed that the highest (83%) and the lowest (41%) mortality of the larvae P.xylostella was induced by fungal BPluS and BNIPTr, respectively. Moreover, the shortest (2.1 days) and the highest (4.3days) lethal times of the infected host were induced by fungal BPluS and BNIPTr, respectively.

scholarly journals Evaluating the virulence of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) and Beauveria bassiana (Ascomycota: Hypocreales) isolates to Arabian rhinoceros beetle, Oryctes agamemnon arabicus

2015 ◽  
Vol 47 (3) ◽  
pp. 117 ◽  
Author(s):  
M.W. Khudhair ◽  
M.Z. Khalaf ◽  
H.F. Alrubeai ◽  
A.K. Shbar ◽  
B.S. Hamad ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Pupal Stage ◽  
Control Treatment ◽  
Rhinoceros Beetle ◽  
Oryctes Agamemnon ◽  
Lethal Time ◽  
Short Time

Virulence of entomopathogenic fungi <em>Metarhizium anisopliae</em> and <em>Beauveria bassiana</em> were tested against Arabian Rhinoceros Beetle, <em>Oryctes agamemnon arabicus</em> larvae. Four concentrations (1×10<sup>5</sup>, 1×10<sup>7</sup>, 1×10<sup>9</sup> and 1×10<sup>11</sup> conidia/mL<sup>–1</sup>) of two locally isolated entomopathogenic fungi spore suspensions were used in this study via larval direct spraying. Results revealed that both isolates can cause high mortality rate reaching 100% after 29 days. However, <em>Beauveria bassiana</em> scored higher mortality rate in short time especially at the concentration of 1×10<sup>11</sup> conidia/ mL<sup>–1</sup> with lethal time (LT)<sub>50</sub> 12.75 and LT<sub>90</sub> 20.00; while, <em>Metarhizium anisopliae</em> caused the higher percentage of malformed adults. Moreover, both isolates affected insect’s life cycle particularly in the pupal stage which was reduced remarkably by almost 50% in comparison with the control treatment.

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