Correlation of HSP70 Expression and Cell Viability Following Thermal Stimulation of Bovine Aortic Endothelial Cells
2005 ◽
Vol 127
(5)
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pp. 751-757
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Keyword(s):
Thermal preconditioning protocols for cardiac cells were identified which produce elevated HSP70 levels while maintaining high cell viability. Bovine aortic endothelial cells were heated with a water bath at temperatures ranging from 44to50°C for periods of 1-30min. Thermal stimulation protocols were determined which induce HSP70 expression levels ranging from 2.3 to 3.6 times the control while maintaining cell viabilities greater than 90%. An Arrhenius injury model fit to the cell damage data yielded values of A=1.4×1066s−1 and Ea=4.1×105J∕mol. Knowledge of the injury parameters and HSP70 kinetics will enhance dosimetry guideline development for thermal stimulation of heat shock proteins expression in cardiac tissue.
1985 ◽
Vol 124
(1)
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pp. 120-124
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1988 ◽
Vol 137
(1)
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pp. 192-198
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1977 ◽
Vol 92
(2)
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pp. 177-183
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1988 ◽
Vol 137
(2)
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pp. 251-262
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1999 ◽
Vol 80
(2)
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pp. 177-180
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1987 ◽
1996 ◽
Vol 271
(2)
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pp. C555-C562
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