Design and Preliminary Testing of Novel Injection Port Contamination Barrier Devices

2017 ◽  
Vol 11 (3) ◽  
Author(s):  
David B. Wax ◽  
Bryan Hill
Keyword(s):  
Adenosine Triphosphate ◽  
Microbial Contamination ◽  
Postoperative Infections ◽  
Protective Shell ◽  
Injection Port ◽  
Preliminary Testing ◽  
Environmental Surfaces ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay

Prior studies have linked microbial contamination of intravenous (IV) ports and stopcocks with postoperative infections. Existing technologies to address contamination are not consistently utilized because of the time and effort they require. Herein, novel barrier devices were created that form a protective shell to passively prevent contact between injection sites and practitioner hands or environmental surfaces while still allowing rapid connection of a syringe for injection of medications via an opening in the shell. Prototypes were tested using a grossly contaminated environment and adenosine triphosphate (ATP)-bioluminescence assay. For eight pairs of unshielded versus shielded IV ports/stopcocks, average contamination was 4102 versus 35 RLU (p < 0.02), respectively, indicating that the devices could significantly reduce IV port/stopcock contamination.

Comparison of Fluorescent Marker Systems with 2 Quantitative Methods of Assessing Terminal Cleaning Practices

2011 ◽  
Vol 32 (12) ◽  
pp. 1187-1193 ◽  
Author(s):  
John M. Boyce ◽  
Nancy L. Havill ◽  
Heather L. Havill ◽  
Elise Mangione ◽  
Diane G. Dumigan ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Quantitative Methods ◽  
Convenience Sample ◽  
Fluorescent Marker ◽  
Fluorescent Markers ◽  
Before And After ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay ◽  
Community Teaching

Objective.To compare fluorescent markers with aerobic colony counts (ACCs) and an adenosine triphosphate (ATP) bioluminescence assay system for assessing terminal cleaning practices.Design.A prospective observational survey.Setting.A 500-bed university-affiliated community teaching hospital.Methods.In a convenience sample of 100 hospital rooms, 5 high-touch surfaces were marked with fluorescent markers before terminal cleaning and checked after cleaning to see whether the marker had been entirely or partially removed. ACC and ATP readings were performed on the same surfaces before and after terminal cleaning.Results.Overall, 378 (76%) of 500 surfaces were classified as having been cleaned according to fluorescent markers, compared with 384 (77%) according to ACC criteria and 225 (45%) according to ATP criteria. Of 382 surfaces classified as not clean according to ATP criteria before terminal cleaning, those with the marker removed were significantly more likely than those with the marker partially removed to be classified as clean according to ATP criteria (P = .003).Conclusions.Fluorescent markers are useful in determining how frequently high-touch surfaces are wiped during terminal cleaning. However, contaminated surfaces classified as clean according to fluorescent marker criteria after terminal cleaning were significantly less likely to be classified as clean according to ACC and ATP assays.

Comparison of a Rapid ATP Bioluminescence Assay and Standard Plate Count Methods for Assessing Microbial Contamination of Consumers' Refrigerators

2006 ◽  
Vol 69 (10) ◽  
pp. 2534-2538 ◽  
Author(s):  
FUR-CHI CHEN ◽  
SANDRIA L. GODWIN
Keyword(s):  
Microbial Contamination ◽  
Field Studies ◽  
Plate Count ◽  
Culture Methods ◽  
Psychrotrophic Bacteria ◽  
Standard Plate Count ◽  
Plate Count Agar ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay

The feasibility of using an ATP bioluminescence assay for assessing microbial contamination of home refrigerators was evaluated and compared with the standard culture methods. Samples of refrigerator surfaces were collected from 123 households by swabbing an area of 100 cm2 on three locations in the refrigerator with premoisturized sterile swabs. Microbial contaminations were determined by aerobic plate count (APC; incubated at 35°C for 48 h) and psychrotrophic plate count (PPC; incubated at 7°C for 10 days) on plate count agar. The results were compared to the readings from the microbial ATP (mATP) bioluminescence assay. The correlation coefficient (r) between mATP and PPC (r = 0.851) was slightly higher than that between mATP and APC (r = 0.823). Our results indicated a potential discrepancy in the population of mesophilic and psychrotrophic bacteria in the refrigerator samples. Nevertheless, mATP appeared to be a reliable indication of the average of APC and PPC (r = 0.895). The mATP bioluminescence assay would provide a rapid and convenient test for researchers in field studies to assess microbial contamination in refrigerators.

Adenosine Triphosphate Bioluminescence for Hygiene Monitoring in Health Care Institutions

1994 ◽  
Vol 57 (6) ◽  
pp. 509-513 ◽  
Author(s):  
KLAUS SEEGER ◽  
MANSEL W. GRIFFITHS
Keyword(s):  
Health Care ◽  
Adenosine Triphosphate ◽  
Meat Product ◽  
Plate Count ◽  
Standard Plate Count ◽  
Atp Assay ◽  
Health Care Institutions ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay

An investigation was conducted to assess the practical use of an adenosine triphosphate (ATP) bioluminescence assay to evaluate the effectiveness of cleaning and sanitizing meat slicers in eight health care institutions. The ATP bioluminescence assay was compared to conventional swabbing techniques using standard plate count to enumerate microbial load. Assays were performed on meat slicers before use, after slicing a meat product and after sanitizing. There was a general overall agreement in results obtained by both methods but the ATP assay gave a better indication of the cleanliness of the meat slicer as it was able to detect the presence of meat residues left on the blade after improper sanitation. Results were available within 5 min using the ATP bioluminescence method, thus providing an opportunity for immediate remedial action.

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