Adenosine Triphosphate Bioluminescence for Hygiene Monitoring in Health Care Institutions

1994 ◽  
Vol 57 (6) ◽  
pp. 509-513 ◽  
Author(s):  
KLAUS SEEGER ◽  
MANSEL W. GRIFFITHS
Keyword(s):  
Health Care ◽  
Adenosine Triphosphate ◽  
Meat Product ◽  
Plate Count ◽  
Standard Plate Count ◽  
Atp Assay ◽  
Health Care Institutions ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay

An investigation was conducted to assess the practical use of an adenosine triphosphate (ATP) bioluminescence assay to evaluate the effectiveness of cleaning and sanitizing meat slicers in eight health care institutions. The ATP bioluminescence assay was compared to conventional swabbing techniques using standard plate count to enumerate microbial load. Assays were performed on meat slicers before use, after slicing a meat product and after sanitizing. There was a general overall agreement in results obtained by both methods but the ATP assay gave a better indication of the cleanliness of the meat slicer as it was able to detect the presence of meat residues left on the blade after improper sanitation. Results were available within 5 min using the ATP bioluminescence method, thus providing an opportunity for immediate remedial action.

Use of a Rapid Microbial ATP Bioluminescence Assay to Detect Contamination on Beef and Pork Carcasses†

1995 ◽  
Vol 58 (7) ◽  
pp. 770-775 ◽  
Author(s):  
GREGORY R. SIRAGUSA ◽  
CATHERINE N. CUTTER ◽  
WARREN J. DORSA ◽  
MOHAMMAD KOOHMARAIE
Keyword(s):  
Plate Count ◽  
Assay Sensitivity ◽  
Standard Plate Count ◽  
Critical Control Points ◽  
Processing Plants ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Commercial Processing ◽  
Atp Bioluminescence Assay

A new microbial ATP bioluminescence assay was shown to be an accurate and rapid method to determine the levels of generic bacterial contamination on beef (n = 400 and pork (n = 320) carcasses sampled in commercial processing plants. Based on in vitro fecal dilution studies, the rapid microbial ATP (R-mATP) assay is as accurate as the standard plate count method for estimating bacteria in bovine or porcine fecal samples. The correlations (r) between the R-mATP assay and the standard aerobic plate count for beef and pork carcasses sampled in commercial processing were 0.91 and 0.93, respectively. A segmented-model statistical approach to determine the lower limits of assay sensitivity was developed. By using this model to analyze the in-plant data, the R-mATP test responded in a linear fashion to levels of microbial contamination of > log10 2.0 aerobic CFU/cm2 on beef carcasses and of > log10 3.2 aerobic CFU/cm2 for pork carcasses. The R-mATP assay requires approximately 5 min to complete, including sampling. Given the rapidity and accuracy of the assay, processors interested in monitoring critical control points in the slaughter process could potentially use the R-mATP assay to monitor microbiological prevention and intervention procedures for minimizing carcass contamination.

Comparison of a Rapid ATP Bioluminescence Assay and Standard Plate Count Methods for Assessing Microbial Contamination of Consumers' Refrigerators

2006 ◽  
Vol 69 (10) ◽  
pp. 2534-2538 ◽  
Author(s):  
FUR-CHI CHEN ◽  
SANDRIA L. GODWIN
Keyword(s):  
Microbial Contamination ◽  
Field Studies ◽  
Plate Count ◽  
Culture Methods ◽  
Psychrotrophic Bacteria ◽  
Standard Plate Count ◽  
Plate Count Agar ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay

The feasibility of using an ATP bioluminescence assay for assessing microbial contamination of home refrigerators was evaluated and compared with the standard culture methods. Samples of refrigerator surfaces were collected from 123 households by swabbing an area of 100 cm2 on three locations in the refrigerator with premoisturized sterile swabs. Microbial contaminations were determined by aerobic plate count (APC; incubated at 35°C for 48 h) and psychrotrophic plate count (PPC; incubated at 7°C for 10 days) on plate count agar. The results were compared to the readings from the microbial ATP (mATP) bioluminescence assay. The correlation coefficient (r) between mATP and PPC (r = 0.851) was slightly higher than that between mATP and APC (r = 0.823). Our results indicated a potential discrepancy in the population of mesophilic and psychrotrophic bacteria in the refrigerator samples. Nevertheless, mATP appeared to be a reliable indication of the average of APC and PPC (r = 0.895). The mATP bioluminescence assay would provide a rapid and convenient test for researchers in field studies to assess microbial contamination in refrigerators.

Study on the Detection of Total Colony in Medical and Health Environment Based on ATP Bioluminescence

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
Zhe Li
Keyword(s):  
Culture Method ◽  
Pass Rate ◽  
Plate Count ◽  
Count Method ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Plate Count Method ◽  
The Difference ◽  
Atp Bioluminescence Assay ◽  
Bioluminescence Method

In this paper, the application of ATP fluorescence in the detection of colonies in the health environment of hospitals was studied. Firstly, the principle of ATP bioluminescence method was described. Then, ATP bioluminescence and plate count method were used to test the density of the surface of the objects in selected area, taking the time points 2 hours after disinfection as the time nodes. The results showed that the difference between the qualified rate of ATP bioluminescence assay and the plate count method was statistically significant {P<0.01}. Therefore, ATP bioluminescence method was highly correlated with bacterial culture method. The correlation coefficient of pass rate of the two methods was 0.782, which indicated that there was a positive correlation between the two test results. Besides, the detection results showed that ATP bioluminescence method had higher sensitivity than plate counting method. Therefore, ATP bioluminescence method was more suitable for the rapid detection of the colony of hospital health environment, and helps the hospital to better manage its environmental hygiene conditions. 

scholarly journals No-Touch Automated Disinfection System for Decontamination of Surfaces in Hospitals

2020 ◽  
Vol 17 (14) ◽  
pp. 5131
Author(s):  
Patryk Tarka ◽  
Aneta Nitsch-Osuch
Keyword(s):  
Microbiological Assay ◽  
Acceptable Level ◽  
Hospital Acquired Infections ◽  
Atp Assay ◽  
New Methods ◽  
Colony Forming Units ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay ◽  
Hospital Acquired

Background: Hospital-acquired infections (HAIs) remain a common problem, which suggests that standard decontamination procedures are insufficient. Thus, new methods of decontamination are needed in hospitals. Methods: We assessed the effectiveness of a no-touch automated disinfection (NTD) system in the decontamination of 50 surfaces in 10 hospital rooms. Contamination of surfaces was assessed with a microbiological assay and an ATP bioluminescence assay. Unacceptable contamination was defined as > 100 colony forming units/100 cm2 in the microbiological assay, and as ≥ 250 relative light units in the ATP assay. Results: When measured with the microbiological assay, 11 of 50 surfaces had unacceptable contamination before NTD, and none of the surfaces had unacceptable contamination after NTD (p < 0.001). On the ATP bioluminescence assay, NTD decreased the number of surfaces with unacceptable contamination from 28 to 13, but this effect was non-significant (p = 0.176). On the microbiological assay taken before NTD, the greatest contamination exceeded the acceptable level by more than 11-fold (lamp holder, 1150 CFU/100 cm2). On the ATP bioluminescence assay taken before NTD, the greatest contamination exceeded the acceptable level by more than 43-fold (Ambu bag, 10,874 RLU). Conclusion: NTD effectively reduced microbiological contamination in all hospital rooms. However, when measured with the ATP bioluminescence assay, the reduction of contamination was not significant.

Application of a fluorescent marker with quantitative bioburden methods to assess cleanliness

2018 ◽  
Vol 39 (11) ◽  
pp. 1296-1300 ◽  
Author(s):  
I-Chen Hung ◽  
Hao-Yuan Chang ◽  
Aristine Cheng ◽  
An-Chi Chen ◽  
Ling Ting ◽  
...  
Keyword(s):  
Medical Center ◽  
Academic Medical Center ◽  
Survey Study ◽  
Environmental Cleaning ◽  
Fluorescent Marker ◽  
Atp Assay ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Cross Transmission ◽  
Atp Bioluminescence Assay

AbstractBackgroundImprovement of environmental cleaning in hospitals has been shown to decrease in-hospital cross transmission of pathogens. Several objective methods, including aerobic colony counts (ACCs), the adenosine triphosphate (ATP) bioluminescence assay, and the fluorescent marker method have been developed to assess cleanliness. However, the standard interpretation of cleanliness using the fluorescent marker method remains uncertain.ObjectiveTo assess the fluorescent marker method as a tool for determining the effectiveness of hospital cleaning.DesignA prospective survey study.SettingAn academic medical center.MethodsThe same 10 high-touch surfaces were tested after each terminal cleaning using (1) the fluorescent marker method, (2) the ATP assay, and (3) the ACC method. Using the fluorescent marker method under study, surfaces were classified as totally clean, partially clean, or not clean. The ACC method was used as the standard for comparison.ResultsAccording to the fluorescent marker method, of the 830 high-touch surfaces, 321 surfaces (38.7%) were totally clean (TC group), 84 surfaces (10.1%) were partially clean (PC group), and 425 surfaces (51.2%) were not clean (NC group). The TC group had significantly lower ATP and ACC values (mean ± SD, 428.7 ± 1,180.0 relative light units [RLU] and 15.6 ± 77.3 colony forming units [CFU]/100 cm2) than the PC group (1,386.8 ± 2,434.0 RLU and 34.9 ± 87.2 CFU/100 cm2) and the NC group (1,132.9 ± 2,976.1 RLU and 46.8 ± 119.2 CFU/100 cm2).ConclusionsThe fluorescent marker method provided a simple, reliable, and real-time assessment of environmental cleaning in hospitals. Our results indicate that only a surface determined to be totally clean using the fluorescent marker method could be considered clean.

Comparison of Fluorescent Marker Systems with 2 Quantitative Methods of Assessing Terminal Cleaning Practices

2011 ◽  
Vol 32 (12) ◽  
pp. 1187-1193 ◽  
Author(s):  
John M. Boyce ◽  
Nancy L. Havill ◽  
Heather L. Havill ◽  
Elise Mangione ◽  
Diane G. Dumigan ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Quantitative Methods ◽  
Convenience Sample ◽  
Fluorescent Marker ◽  
Fluorescent Markers ◽  
Before And After ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay ◽  
Community Teaching

Objective.To compare fluorescent markers with aerobic colony counts (ACCs) and an adenosine triphosphate (ATP) bioluminescence assay system for assessing terminal cleaning practices.Design.A prospective observational survey.Setting.A 500-bed university-affiliated community teaching hospital.Methods.In a convenience sample of 100 hospital rooms, 5 high-touch surfaces were marked with fluorescent markers before terminal cleaning and checked after cleaning to see whether the marker had been entirely or partially removed. ACC and ATP readings were performed on the same surfaces before and after terminal cleaning.Results.Overall, 378 (76%) of 500 surfaces were classified as having been cleaned according to fluorescent markers, compared with 384 (77%) according to ACC criteria and 225 (45%) according to ATP criteria. Of 382 surfaces classified as not clean according to ATP criteria before terminal cleaning, those with the marker removed were significantly more likely than those with the marker partially removed to be classified as clean according to ATP criteria (P = .003).Conclusions.Fluorescent markers are useful in determining how frequently high-touch surfaces are wiped during terminal cleaning. However, contaminated surfaces classified as clean according to fluorescent marker criteria after terminal cleaning were significantly less likely to be classified as clean according to ACC and ATP assays.

Design and Preliminary Testing of Novel Injection Port Contamination Barrier Devices

2017 ◽  
Vol 11 (3) ◽  
Author(s):  
David B. Wax ◽  
Bryan Hill
Keyword(s):  
Adenosine Triphosphate ◽  
Microbial Contamination ◽  
Postoperative Infections ◽  
Protective Shell ◽  
Injection Port ◽  
Preliminary Testing ◽  
Environmental Surfaces ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay

Prior studies have linked microbial contamination of intravenous (IV) ports and stopcocks with postoperative infections. Existing technologies to address contamination are not consistently utilized because of the time and effort they require. Herein, novel barrier devices were created that form a protective shell to passively prevent contact between injection sites and practitioner hands or environmental surfaces while still allowing rapid connection of a syringe for injection of medications via an opening in the shell. Prototypes were tested using a grossly contaminated environment and adenosine triphosphate (ATP)-bioluminescence assay. For eight pairs of unshielded versus shielded IV ports/stopcocks, average contamination was 4102 versus 35 RLU (p < 0.02), respectively, indicating that the devices could significantly reduce IV port/stopcock contamination.

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