Evaluation of the Relationship between the Adenosine  Triphosphate (ATP) Bioluminescence Assay and the  Presence of Bacillus anthracis Spores and Vegetative Cells

Objective.To compare fluorescent markers with aerobic colony counts (ACCs) and an adenosine triphosphate (ATP) bioluminescence assay system for assessing terminal cleaning practices.Design.A prospective observational survey.Setting.A 500-bed university-affiliated community teaching hospital.Methods.In a convenience sample of 100 hospital rooms, 5 high-touch surfaces were marked with fluorescent markers before terminal cleaning and checked after cleaning to see whether the marker had been entirely or partially removed. ACC and ATP readings were performed on the same surfaces before and after terminal cleaning.Results.Overall, 378 (76%) of 500 surfaces were classified as having been cleaned according to fluorescent markers, compared with 384 (77%) according to ACC criteria and 225 (45%) according to ATP criteria. Of 382 surfaces classified as not clean according to ATP criteria before terminal cleaning, those with the marker removed were significantly more likely than those with the marker partially removed to be classified as clean according to ATP criteria (P = .003).Conclusions.Fluorescent markers are useful in determining how frequently high-touch surfaces are wiped during terminal cleaning. However, contaminated surfaces classified as clean according to fluorescent marker criteria after terminal cleaning were significantly less likely to be classified as clean according to ACC and ATP assays.

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Design and Preliminary Testing of Novel Injection Port Contamination Barrier Devices

Journal of Medical Devices ◽

10.1115/1.4036026 ◽

2017 ◽

Vol 11 (3) ◽

Cited By ~ 1

Author(s):

David B. Wax ◽

Bryan Hill

Keyword(s):

Adenosine Triphosphate ◽

Microbial Contamination ◽

Postoperative Infections ◽

Protective Shell ◽

Injection Port ◽

Preliminary Testing ◽

Environmental Surfaces ◽

Atp Bioluminescence ◽

Bioluminescence Assay ◽

Atp Bioluminescence Assay

Prior studies have linked microbial contamination of intravenous (IV) ports and stopcocks with postoperative infections. Existing technologies to address contamination are not consistently utilized because of the time and effort they require. Herein, novel barrier devices were created that form a protective shell to passively prevent contact between injection sites and practitioner hands or environmental surfaces while still allowing rapid connection of a syringe for injection of medications via an opening in the shell. Prototypes were tested using a grossly contaminated environment and adenosine triphosphate (ATP)-bioluminescence assay. For eight pairs of unshielded versus shielded IV ports/stopcocks, average contamination was 4102 versus 35 RLU (p < 0.02), respectively, indicating that the devices could significantly reduce IV port/stopcock contamination.

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Monitoring the effectiveness of daily cleaning practices in an intensive care unit (ICU) setting using an adenosine triphosphate (ATP) bioluminescence assay

American Journal of Infection Control ◽

10.1016/j.ajic.2019.11.031 ◽

2020 ◽

Vol 48 (7) ◽

pp. 757-760

Author(s):

Abhishek Deshpande ◽

Aaron N. Dunn ◽

Jacqueline Fox ◽

Jennifer L. Cadnum ◽

Thriveen S.C. Mana ◽

...

Keyword(s):

Intensive Care Unit ◽

Intensive Care ◽

Adenosine Triphosphate ◽

Atp Bioluminescence ◽

Bioluminescence Assay ◽

Atp Bioluminescence Assay

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Adenosine Triphosphate Bioluminescence for Hygiene Monitoring in Health Care Institutions

Journal of Food Protection ◽

10.4315/0362-028x-57.6.509 ◽

1994 ◽

Vol 57 (6) ◽

pp. 509-513 ◽

Cited By ~ 21

Author(s):

KLAUS SEEGER ◽

MANSEL W. GRIFFITHS

Keyword(s):

Health Care ◽

Adenosine Triphosphate ◽

Meat Product ◽

Plate Count ◽

Standard Plate Count ◽

Atp Assay ◽

Health Care Institutions ◽

Atp Bioluminescence ◽

Bioluminescence Assay ◽

Atp Bioluminescence Assay

An investigation was conducted to assess the practical use of an adenosine triphosphate (ATP) bioluminescence assay to evaluate the effectiveness of cleaning and sanitizing meat slicers in eight health care institutions. The ATP bioluminescence assay was compared to conventional swabbing techniques using standard plate count to enumerate microbial load. Assays were performed on meat slicers before use, after slicing a meat product and after sanitizing. There was a general overall agreement in results obtained by both methods but the ATP assay gave a better indication of the cleanliness of the meat slicer as it was able to detect the presence of meat residues left on the blade after improper sanitation. Results were available within 5 min using the ATP bioluminescence method, thus providing an opportunity for immediate remedial action.

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