Laser Sharpening of Carbon Fiber Microelectrode Arrays for Brain Recording

Author(s):  
Tianshu Dong ◽  
Lei Chen ◽  
Albert Shih

Abstract Microwire microelectrode arrays (MEAs) are implanted in the brain for recording neuron activities to study the brain functioning mechanism. Among various microwire materials that had been applied, carbon fiber is outstanding due to its small footprint (6–7 μm), relatively high Young’s modulus, and low electrical resistance. Tips of microwire in MEAs are often sharpened to reduce insertion force. Currently, carbon fiber MEAs are sharpened with either torch burning, which can only give a uniform length of wires in an array, or electrical discharge machining (EDM), which requires circuit connection with each single carbon fiber. The sharp tip results from intense burning induced by a flame or spark, leading to poor repeatability and controllability of the sharp tip geometry. In this paper, a laser-based, non-contact carbon fiber sharpening method is proposed, which enables controllable and repeatable production of carbon fiber MEAs of custom electrode lengths, insulation stripping lengths, and sharpened tips. Path of laser movement is designed according to desired array pattern. Variation in tip geometry can be accomplished by changing laser output power and moving speed. Test with different laser parameters (output power and moving speed) were conducted. Tip sharpening results were evaluated and analyzed in terms of tip geometry and insulation stripping length. Results showed that to achieve the desired MEA with sharper tip and shorter insulation stripping length, a higher laser power with faster moving speed is preferred.

2020 ◽  
Vol 8 (4) ◽  
Author(s):  
Tianshu Dong ◽  
Lei Chen ◽  
Albert Shih

Abstract Microwire microelectrode arrays (MEAs) are implanted in the brain for recording neuron activities to study the brain function. Among various microwire materials, carbon fiber stands out due to its small diameter (5–10 μm), relatively high Young's modulus, and low electrical resistance. Microwire tips in MEAs are often sharpened to reduce the insertion force and prevent the thin microwires from buckling. Currently, carbon fiber MEAs are sharpened by either torch burning, which limits the positions of wire tips to a water bath surface plane, or electrical discharge machining, which is difficult to implement to the nonelectrically conductive carbon fiber with parylene-C insulation. A laser-based carbon fiber sharpening method proposed in this study enables the fabrication of carbon fiber MEAs with sharp tips and custom lengths. Experiments were conducted to study effects of laser input voltage and transverse speed on carbon fiber tip geometry. Results of the tip sharpness and stripped length of the insulation as well as the electrochemical impedance spectroscopy measurement at 1 kHz were evaluated and analyzed. The laser input voltage and traverse speed have demonstrated to be critical for the sharp tip, short stripped length, and low electrical impedance of the carbon fiber electrode for brain recording MEAs. A carbon fiber MEA with custom electrode lengths was fabricated to validate the laser-based approach.


2016 ◽  
Vol 13 (6) ◽  
pp. 066002 ◽  
Author(s):  
Paras R Patel ◽  
Huanan Zhang ◽  
Matthew T Robbins ◽  
Justin B Nofar ◽  
Shaun P Marshall ◽  
...  

Author(s):  
Kristen N. Reikersdorfer ◽  
Andrea K. Stacy ◽  
David A. Bressler ◽  
Lauren S. Hayashi ◽  
Keith B. Hengen ◽  
...  

The Analyst ◽  
2016 ◽  
Vol 141 (23) ◽  
pp. 6416-6421 ◽  
Author(s):  
R. Asri ◽  
B. O'Neill ◽  
J. C. Patel ◽  
K. A. Siletti ◽  
M. E. Rice

The study of transmitter interactions in the brain requires methodology to detect stimulus-driven neurotransmitter release. This report introduces an enzyme-coated 7 μm carbon-fiber microelectrode used with fast-scan cyclic voltammetry to detect evoked acetylcholine release in mouse brain slices.


2018 ◽  
Author(s):  
Rachel S. Zoll ◽  
Craig B. Schindler ◽  
Travis L. Massey ◽  
Daniel S. Drew ◽  
Michel M. Maharbiz ◽  
...  

AbstractMicrowire and microelectrode arrays used for cortical neural recording typically consist of tens to hundreds of recording sites, but often only a fraction of these sites are in close enough proximity to firing neurons to record single-unit activity. Recent work has demonstrated precise, depth-controllable mechanisms for the insertion of single neural recording electrodes, but these methods are mostly only capable of inserting electrodes which elicit adverse biological response. We present an electrostatic-based actuator capable of inserting individual carbon fiber microelectrodes which elicit minimal to no adverse biological response. The device is shown to insert a carbon fiber recording electrode into an agar brain phantom and can record an artificial neural signal in saline. This technique provides a platform generalizable to many microwire-style recording electrodes.


2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Ahmad A. Jiman ◽  
David C. Ratze ◽  
Elissa J. Welle ◽  
Paras R. Patel ◽  
Elizabeth C. Bottorff ◽  
...  

2020 ◽  
Author(s):  
Ahmad Jiman ◽  
David Ratze ◽  
Elissa Welle ◽  
Paras Patel ◽  
Julianna Richie ◽  
...  

This protocol is for obtaining physiological action potential recordings in rat vagus nerves using carbon fiber microelectrode arrays (CFMAs) in spontaneous and blood glucose and breathing modulated conditions. The rats were anesthetized with isoflurane, which maintained consistent and stable depth of anesthesia for recording vagal nerve activity with ultra-small carbon fibers. Blood glucose levels were modulated by intraperitoneal (IP) injection of glucose, insulin, or 2-deoxy-D-glucose (2-DG). Breathing was modulated by increasing anesthesia depth. Carbon fiber microelectrode arrays are available through the Multimodal Integrated Neural Technologies (MINT) technology hub (https://mint.engin.umich.edu/), which is supported by the National Science Foundation (Award 1707316). This research was also supported by the National Institute of Health SPARC Program (Award OT2OD024907).


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