Intraneural Recordings in Rat Vagus Nerves Using Carbon Fiber Microelectrode Arrays v1
This protocol is for obtaining physiological action potential recordings in rat vagus nerves using carbon fiber microelectrode arrays (CFMAs) in spontaneous and blood glucose and breathing modulated conditions. The rats were anesthetized with isoflurane, which maintained consistent and stable depth of anesthesia for recording vagal nerve activity with ultra-small carbon fibers. Blood glucose levels were modulated by intraperitoneal (IP) injection of glucose, insulin, or 2-deoxy-D-glucose (2-DG). Breathing was modulated by increasing anesthesia depth. Carbon fiber microelectrode arrays are available through the Multimodal Integrated Neural Technologies (MINT) technology hub (https://mint.engin.umich.edu/), which is supported by the National Science Foundation (Award 1707316). This research was also supported by the National Institute of Health SPARC Program (Award OT2OD024907).