Augmented and Magnetically-Aligned Type I Collagen-GAG Scaffolds for Cartilage Tissue Engineering

2013 ◽  
Author(s):  
Tyler Novak ◽  
Sherry Voytik-Harbin ◽  
Corey P. Neu
Keyword(s):  
Tissue Engineering ◽  
Type I Collagen ◽  
Cartilage Tissue Engineering ◽  
Cartilage Degeneration ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Type I ◽  
Limited Success ◽  
Research Based Interventions ◽  
Magnetically Aligned

Osteoarthritis (OA) affects over 27 million Americans, causing an annual economic burden of over $300 million [1]. Left untreated, local cartilage defects promote cartilage degeneration and serve as a target for clinical and research based interventions [2]. While current treatments have limited success and result in recurring symptoms [3], tissue engineering solutions are promising for cartilage repair.

Type I collagen scaffold with WNT5A plasmid for in situ cartilage tissue engineering

2021 ◽  
pp. 1-12
Author(s):  
Ruo-Fu Tang ◽  
Xiao-zhong Zhou ◽  
Lie Niu ◽  
Yi-Ying Qi
Keyword(s):  
Tissue Engineering ◽  
Cartilage Repair ◽  
Type I Collagen ◽  
Cartilage Tissue Engineering ◽  
Collagen Scaffold ◽  
Defect Group ◽  
Cartilage Tissue ◽  
Type I ◽  
Type Ii ◽  
Osteochondral Defects

BACKGROUND: Cartilage tissue lacks the ability to heal. Cartilage tissue engineering using cell-free scaffolds has been increasingly used in recent years. OBJECTIVE: This study describes the use of a type I collagen scaffold combined with WNT5A plasmid to promote chondrocyte proliferation and differentiation in a rabbit osteochondral defect model. METHODS: Type I collagen was extracted and fabricated into a collagen scaffold. To improve gene transfection efficiency, a cationic chitosan derivative N,N,N-trimethyl chitosan chloride (TMC) vector was used. A solution of TMC/WNT5A complexes was adsorbed to the collagen scaffold to prepare a WNT5A scaffold. Osteochondral defects were created in the femoral condyles of rabbits. The rabbits were divided into defect, scaffold, and scaffold with WNT5A groups. At 6 and 12 weeks after creation of the osteochondral defects, samples were collected from all groups for macroscopic observation and gene expression analysis. RESULTS: Samples from the defect group exhibited incomplete cartilage repair, while those from the scaffold and scaffold with WNT5A groups exhibited “preliminary cartilage” covering the defect. Cartilage regeneration was superior in the scaffold with WNT5A group compared to the scaffold group. Safranin O staining revealed more proteoglycans in the scaffold and scaffold with WNT5A groups compared to the defect group. The expression levels of aggrecan, collagen type II, and SOX9 genes were significantly higher in the scaffold with WNT5A group compared to the other two groups. CONCLUSIONS: Type I collagen scaffold showed effective adsorption and guided the three-dimensional arrangement of stem cells. WNT5A plasmid promoted cartilage repair by stimulating the expression of aggrecan, type II collagen, and SOX9 genes and proteins, as well as inhibiting cartilage hypertrophy.

scholarly journals Type I Collagen and heparan sulfate scaffolds support human chondrogenesis for cartilage tissue engineering

2012 ◽  
Vol 20 ◽  
pp. S271-S272
Author(s):  
S. Díaz-Prado ◽  
E. Muiños-López ◽  
T. Hermida-Gómez ◽  
I. Fuentes-Boquete ◽  
P. Esbrit ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Heparan Sulfate ◽  
Type I Collagen ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Type I

Human Cartilage Tissue Engineering Using Type I Collagen/Heparan Sulfate Scaffolds

2015 ◽  
Vol 03 (02) ◽  
Author(s):  
C. Sanjurjo Rodríguez ◽  
A.H. Martínez Sánchez ◽  
T. Hermida Gómez ◽  
I. Fuentes Boquete
Keyword(s):  
Tissue Engineering ◽  
Heparan Sulfate ◽  
Type I Collagen ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Type I ◽  
Human Cartilage

scholarly journals Efficacy of collagen and alginate hydrogels for the prevention of rat chondrocyte dedifferentiation

2018 ◽  
Vol 9 ◽  
pp. 204173141880243 ◽  
Author(s):  
Guang-Zhen Jin ◽  
Hae-Won Kim
Keyword(s):  
Tissue Engineering ◽  
Type I Collagen ◽  
Cartilage Tissue Engineering ◽  
Collagen Gel ◽  
Type Ii Collagen ◽  
Cartilage Tissue ◽  
Type I ◽  
Type Ii ◽  
Chondrogenic Phenotype

Dedifferentiation of chondrocytes remains a major problem in cartilage tissue engineering. The development of hydrogels that can preserve chondrogenic phenotype and prevent chondrocyte dedifferentiation is a meaningful strategy to solve dedifferentiation problem of chondrocytes. In the present study, three gels were prepared (alginate gel (Alg gel), type I collagen gel (Col gel), and their combination gel (Alg/Col gel)), and the in vitro efficacy of chondrocytes culture while preserving their phenotypes was investigated. While Col gel became substantially contracted with time, the cells encapsulated in Alg gel preserved the shape over the culture period of 14 days. The mechanical and cell-associated contraction behaviors of Alg/Col gel were similar to those of Alg. The cells in Alg and Alg/Col gels exhibited round morphology, whereas those in Col gel became elongated (i.e. fibroblast-like) during cultures. The cells proliferated with time in all gels with the highest proliferation being attained in Col gel. The expression of chondrogenic genes, including SOX9, type II collagen, and aggrecan, was significantly up-regulated in Alg/Col gel and Col gel, particularly in Col gel. However, the chondrocyte dedifferentiation markers, type I collagen and alkaline phosphatase ( ALP), were also expressed at significant levels in Col gel, which being contrasted with the events in Alg and Alg/Col gels. The current results suggest the cells cultured in hydrogels can express chondrocyte dedifferentiation markers as well as chondrocyte markers, which draws attention to choose proper hydrogels for chondrocyte-based cartilage tissue engineering.

scholarly journals Cartilage Tissue Engineering Using Thyroid Chondrocytes on a Type I Collagen Matrix

2000 ◽  
Vol 110 (12) ◽  
pp. 2008-2011 ◽  
Author(s):  
Beth A. Wambach ◽  
Herman Cheung ◽  
Gary D. Josephson
Keyword(s):  
Tissue Engineering ◽  
Type I Collagen ◽  
Cartilage Tissue Engineering ◽  
Collagen Matrix ◽  
Cartilage Tissue ◽  
Type I

scholarly journals Numerical Simulation of Electroactive Hydrogels for Cartilage–Tissue Engineering

Materials ◽  
2019 ◽  
Vol 12 (18) ◽  
pp. 2913 ◽  
Author(s):  
Abdul Razzaq Farooqi ◽  
Julius Zimmermann ◽  
Rainer Bader ◽  
Ursula van Rienen
Keyword(s):  
Tissue Engineering ◽  
Electric Stimulation ◽  
Mathematical Formulation ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Hydrogel Scaffolds ◽  
Finite Element Software ◽  
Defect Site ◽  
Articular Cartilage Defects

The intrinsic regeneration potential of hyaline cartilage is highly limited due to the absence of blood vessels, lymphatics, and nerves, as well as a low cell turnover within the tissue. Despite various advancements in the field of regenerative medicine, it remains a challenge to remedy articular cartilage defects resulting from trauma, aging, or osteoarthritis. Among various approaches, tissue engineering using tailored electroactive scaffolds has evolved as a promising strategy to repair damaged cartilage tissue. In this approach, hydrogel scaffolds are used as artificial extracellular matrices, and electric stimulation is applied to facilitate proliferation, differentiation, and cell growth at the defect site. In this regard, we present a simulation model of electroactive hydrogels to be used for cartilage–tissue engineering employing open-source finite-element software FEniCS together with a Python interface. The proposed mathematical formulation was first validated with an example from the literature. Then, we computed the effect of electric stimulation on a circular hydrogel sample that served as a model for a cartilage-repair implant.

scholarly journals Anisotropic Shape-Memory Alginate Scaffolds Functionalized with Either Type I or Type II Collagen for Cartilage Tissue Engineering

2017 ◽  
Vol 23 (1-2) ◽  
pp. 55-68 ◽  
Author(s):  
Henrique V. Almeida ◽  
Binulal N. Sathy ◽  
Ivan Dudurych ◽  
Conor T. Buckley ◽  
Fergal J. O'Brien ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Shape Memory ◽  
Cartilage Tissue Engineering ◽  
Type Ii Collagen ◽  
Cartilage Tissue ◽  
Type I ◽  
Type Ii

Treatment of articular cartilage defects in horses with polymer-based cartilage tissue engineering grafts

Biomaterials ◽  
2006 ◽  
Vol 27 (14) ◽  
pp. 2882-2889 ◽  
Author(s):  
Dirk Barnewitz ◽  
Michaela Endres ◽  
Ina Krüger ◽  
Anja Becker ◽  
Jürgen Zimmermann ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Articular Cartilage ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Articular Cartilage Defects

scholarly journals Bioprinting of a Zonal-Specific Cell Density Scaffold: A Biomimetic Approach for Cartilage Tissue Engineering

2021 ◽  
Vol 11 (17) ◽  
pp. 7821
Author(s):  
Angeliki Dimaraki ◽  
Pedro J. Díaz-Payno ◽  
Michelle Minneboo ◽  
Mahdiyeh Nouri-Goushki ◽  
Maryam Hosseini ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Articular Cartilage ◽  
Cell Density ◽  
Articular Chondrocytes ◽  
Cartilage Tissue Engineering ◽  
Smooth Transition ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Specific Cell ◽  
Cell Densities

The treatment of articular cartilage defects remains a significant clinical challenge. This is partially due to current tissue engineering strategies failing to recapitulate native organization. Articular cartilage is a graded tissue with three layers exhibiting different cell densities: the superficial zone having the highest density and the deep zone having the lowest density. However, the introduction of cell gradients for cartilage tissue engineering, which could promote a more biomimetic environment, has not been widely explored. Here, we aimed to bioprint a scaffold with different zonal cell densities to mimic the organization of articular cartilage. The scaffold was bioprinted using an alginate-based bioink containing human articular chondrocytes. The scaffold design included three cell densities, one per zone: 20 × 106 (superficial), 10 × 106 (middle), and 5 × 106 (deep) cells/mL. The scaffold was cultured in a chondrogenic medium for 25 days and analyzed by live/dead assay and histology. The live/dead analysis showed the ability to generate a zonal cell density with high viability. Histological analysis revealed a smooth transition between the zones in terms of cell distribution and a higher sulphated glycosaminoglycan deposition in the highest cell density zone. These findings pave the way toward bioprinting complex zonal cartilage scaffolds as single units, thereby advancing the translation of cartilage tissue engineering into clinical practice.

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