Polypyrrole Bridge as a Support for Alamethicin-Reconstituted Planar Bilayer Lipid Membranes

2011 ◽  
Author(s):  
Robert Northcutt ◽  
Vishnu-Baba Sundaresan ◽  
Sergio Salinas ◽  
Hao Zhang
Keyword(s):  
Ion Transport ◽  
Conducting Polymer ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Active Material ◽  
Experimental Studies ◽  
Bilayer Lipid Membrane ◽  
Electrical Impedance Spectroscopy ◽  
Bilayer Lipid ◽  
Ionic Components

Conducting polymer actuators and sensors utilize electrochemical reactions and associated ion transport at the polymer-electrolyte interface for their engineering function. Similarly, a bioderived active material utilizes ion transport through a protein and across a bilayer lipid membrane for sensing and actuation functions. Inspired by the similarity in ion transport process in a bilayer lipid membrane (BLM) and conducting polymers, we propose to build an integrated ionic device in which the ion transport through the protein in the bilayer lipid membrane regulates the electrolytic and mechanical properties of the conducting polymer. This article demonstrates the fabrication and characterization of a DPhPC planar BLM reconstituted with alamethicin and supported on a polypyrrole bridge measuring 100 μm × 500 μm and formed across micro-fabricated gold pads. The assembly is supported on silicon dioxide coated wafers and packaged into an electronic-ionic package for electrochemical characterization. The various ionic components in the integrated ionic device are characterized using electrical impedance spectroscopy (EIS), cyclic voltammetry (CV), and chronoamperometry (CA) measurements. The results from our experimental studies demonstrate the procedure to fabricate a rugged electro active polymer supported BLM that will serve as a platform for chemical, bioelectrical sensing and VOC detection.

Ion transport across a bilayer lipid membrane facilitated by gramicidin A – Effect of counter anions on the cation transport

2006 ◽  
Vol 595 (1) ◽  
pp. 53-59 ◽  
Author(s):  
Osamu Shirai ◽  
Yumi Yoshida ◽  
Sorin Kihara ◽  
Toshihiko Ohnuki ◽  
Akihiro Uehara ◽  
...  
Keyword(s):  
Ion Transport ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Cation Transport ◽  
Gramicidin A ◽  
Bilayer Lipid

Self-Healing Bilayer Lipid Membranes Formed Over Synthetic Substrates

2008 ◽  
Author(s):  
M. Austin Creasy ◽  
Donald J. Leo
Keyword(s):  
Self Assembly ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Unique Property ◽  
Bilayer Lipid Membranes ◽  
Self Healing ◽  
Electrical Field ◽  
Bilayer Lipid ◽  
Recent Experimental Study

Biological systems demonstrate autonomous healing of damage and are an inspiration for developing self-healing materials. Our recent experimental study has demonstrated that a bilayer lipid membrane (BLM), also called a black lipid membrane, has the ability to self-heal after mechanical failure. These molecules have a unique property that they spontaneously self assembly into organized structures in an aqueous medium. The BLM forms an impervious barrier to ions and fluid between two volumes and strength of the barrier is dependent on the pressure and electrical field applied to the membrane. A BLM formed over an aperture on a silicon substrate is shown to self-heal for 5 pressurization failure cycles.

Ion Transport across Planar Bilayer Lipid Membrane Driven by D-Fructose Dehydrogenase-catalyzed Electron Transport

2011 ◽  
Vol 40 (5) ◽  
pp. 486-488 ◽  
Author(s):  
Keisuke Sasakura ◽  
Osamu Shirai ◽  
Kei Hichiri ◽  
Maiko Goda-Tsutsumi ◽  
Seiya Tsujimura ◽  
...  
Keyword(s):  
Electron Transport ◽  
Ion Transport ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Planar Bilayer ◽  
Bilayer Lipid ◽  
Fructose Dehydrogenase

Potassium ion transport by gramicidin and valinomycin across a Ag(111)-supported tethered bilayer lipid membrane

2008 ◽  
Vol 53 (22) ◽  
pp. 6372-6379 ◽  
Author(s):  
Lucia Becucci ◽  
Massimo Innocenti ◽  
Emanuele Salvietti ◽  
Alessio Rindi ◽  
Irene Pasquini ◽  
...  
Keyword(s):  
Ion Transport ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Potassium Ion ◽  
Bilayer Lipid ◽  
Potassium Ion Transport

Ion transport across a bilayer lipid membrane facilitated by valinomycin

2004 ◽  
Vol 570 (2) ◽  
pp. 219-226 ◽  
Author(s):  
Osamu Shirai ◽  
Hajimu Yamana ◽  
Toshihiko Ohnuki ◽  
Yumi Yoshida ◽  
Sorin Kihara
Keyword(s):  
Ion Transport ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Bilayer Lipid

Electrochemical Analysis of Alamethicin Reconstituted Planar Bilayer Lipid Membranes Supported on Polypyrrole Membranes

2011 ◽  
Author(s):  
Hao Zhang ◽  
Vishnu Baba Sundaresan ◽  
Sergio Salinas ◽  
Robert Northcutt
Keyword(s):  
Energy Conversion ◽  
Conducting Polymer ◽  
Lipid Membranes ◽  
Cell Membranes ◽  
Mechanical Energy ◽  
Hybrid Membrane ◽  
Electrical Impedance Spectroscopy ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid

Conducting polymers possess similarity in ion transport function to cell membranes and perform electro-chemo-mechanical energy conversion. In an in vitro setup, protein-reconstituted bilayer lipid membranes (bioderived membranes)perform similar energy conversion and behave like cell membranes. Inspired by the similarity in ionic function between a conducting polymer membrane and cell membrane, this article presents a thin-film laminated membrane in which alamethicin-reconstituted lipid bilayer membrane is supported on a polypyrrole membrane. Owing to the synthetic and bioderived nature of the components of the membrane, we refer to the laminated membrane as a hybrid bioderived membrane. In this article, we describe the fabrication steps and electrochemical characterization of the hybrid membrane. The fabrication steps include electropolymerization of pyrrole and vesicle fusion to result in a hybrid membrane; and the characterization involves electrical impedance spectroscopy, chronoamperometry and cyclic voltammetry. The resistance and capacitance of BLM have the magnitude of 4.6×109Ω-cm2 and 1.6×10−8 F/cm2.The conductance of alamethicin has the magnitude of 6.4×10−8 S/cm2. The change in ionic conductance of the bioderived membrane is due to the electrical field applied across alamethicin, a voltage-gated protein and produces a measurable change in the ionic concentration of the conducting polymer substrate.

Failure Characteristics of Bilayer Lipid Membranes (BLMs) Formed over a Single Pore

2007 ◽  
Vol 1061 ◽  
Author(s):  
David Hopkinson ◽  
Donald J. Leo
Keyword(s):  
Lipid Membranes ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Maximum Pressure ◽  
Pressure Curve ◽  
Failure Characteristics ◽  
Test Fixture ◽  
Failure Pressure ◽  
Bilayer Lipid ◽  
Experimental Pressure

ABSTRACTA new methodology has been developed to measure the maximum pressure that can be withstood by a bilayer lipid membrane (BLM) formed over porous substrates. A custom test fixture was fabricated to pressurize BLMs in very fine increasing increments until they fail. This experiment was performed on 1-Stearoyl-2-Oleoyl-sn-Glycero-3-Phosphatidylocholine (SOPC) BLMs formed over polycarbonate substrates with a single pore ranging from 5 to 20 microns in diameter. Failure pressure was found to be inversely proportional to pore diameter. The same set of experiments was repeated for BLMs that were formed from a mixture of SOPC and 50 mol% cholesterol (CHOL). The presence of cholesterol was found to increase the failure pressure of the BLMs by 56% on average. A model of the characteristic pressure curve from this experiment was developed based on the pressurization and flow of fluid through a porous substrate. The model was found to accurately fit the experimental pressure curves.

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