Self-Healing Bilayer Lipid Membranes Formed Over Synthetic Substrates

2008 ◽  
Author(s):  
M. Austin Creasy ◽  
Donald J. Leo
Keyword(s):  
Self Assembly ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Unique Property ◽  
Bilayer Lipid Membranes ◽  
Self Healing ◽  
Electrical Field ◽  
Bilayer Lipid ◽  
Recent Experimental Study

Biological systems demonstrate autonomous healing of damage and are an inspiration for developing self-healing materials. Our recent experimental study has demonstrated that a bilayer lipid membrane (BLM), also called a black lipid membrane, has the ability to self-heal after mechanical failure. These molecules have a unique property that they spontaneously self assembly into organized structures in an aqueous medium. The BLM forms an impervious barrier to ions and fluid between two volumes and strength of the barrier is dependent on the pressure and electrical field applied to the membrane. A BLM formed over an aperture on a silicon substrate is shown to self-heal for 5 pressurization failure cycles.

Failure Characteristics of Bilayer Lipid Membranes (BLMs) Formed over a Single Pore

2007 ◽  
Vol 1061 ◽  
Author(s):  
David Hopkinson ◽  
Donald J. Leo
Keyword(s):  
Lipid Membranes ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Maximum Pressure ◽  
Pressure Curve ◽  
Failure Characteristics ◽  
Test Fixture ◽  
Failure Pressure ◽  
Bilayer Lipid ◽  
Experimental Pressure

ABSTRACTA new methodology has been developed to measure the maximum pressure that can be withstood by a bilayer lipid membrane (BLM) formed over porous substrates. A custom test fixture was fabricated to pressurize BLMs in very fine increasing increments until they fail. This experiment was performed on 1-Stearoyl-2-Oleoyl-sn-Glycero-3-Phosphatidylocholine (SOPC) BLMs formed over polycarbonate substrates with a single pore ranging from 5 to 20 microns in diameter. Failure pressure was found to be inversely proportional to pore diameter. The same set of experiments was repeated for BLMs that were formed from a mixture of SOPC and 50 mol% cholesterol (CHOL). The presence of cholesterol was found to increase the failure pressure of the BLMs by 56% on average. A model of the characteristic pressure curve from this experiment was developed based on the pressurization and flow of fluid through a porous substrate. The model was found to accurately fit the experimental pressure curves.

Evaluating the Failure Pressure of Bilayer Lipid Membranes by a Controllable Electromagnetic Test Fixture

Aerospace ◽  
2006 ◽  
Author(s):  
David P. Hopkinson ◽  
Raffaella De Vita ◽  
Donald J. Leo
Keyword(s):  
Internal Pressure ◽  
Lipid Membranes ◽  
Fluid Transport ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Test Fixture ◽  
Failure Pressure ◽  
Bilayer Lipid ◽  
Electromagnetic Test ◽  
Series Of Experiments

The motion of plants is the inspiration for a new biomimetic actuator that uses fluid transport across a bilayer lipid membrane (BLM) to create internal pressure and cause displacement in the actuator. In order for the actuator to be viable the BLM must be able to withstand this internal pressure without failing. In a previous study, BLMs were formed over a porous polycarbonate substrate and a hydrostatic pressure was applied to the BLM and gradually increased until failure. This test was performed over different pore sizes to measure the failure pressure of the BLM as a function of pore radius. To improve this series of experiments, a new test fixture has been developed that will allow for a more precise measurement of the failure pressure as well as an expanded range of failure pressures. It is computer controlled, using an electromagnetic actuator to pressurize the BLMs and a pressure transducer to monitor pressure. The design scheme for this test fixture and some preliminary results will be presented.

Microfluidic Emulsion Generation and Trapping Enabling Droplet-Interfaced Bilayer Lipid Membrane Arrays

2009 ◽  
Author(s):  
C. Shao ◽  
D. L. DeVoe
Keyword(s):  
Lipid Bilayers ◽  
Single Molecule ◽  
Protein Interactions ◽  
Lipid Membranes ◽  
High Throughput Screening ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Single Molecule Level ◽  
Bilayer Lipid ◽  
Ion Translocation

Freestanding bilayer lipid membranes provide an exceptional platform for measurements of lipid/protein interactions and ion translocation events at the single molecule level. For drug screening applications, large arrays of individual bilayer supports are required. However, an effective method for generating, stabilizing, and monitoring arrays of lipid bilayers remains elusive. Here we investigate a novel approach towards the facile generation of bilayer arrays for high throughput screening. The approach takes advantage of fundamental microfluidic capabilities by combining an emulsion generator with droplet-interfaced membrane formation, allowing for fully-automated production of membrane arrays whose density is, in principle, unlimited.

INFLUENCE OF ZINC OXIDE NANOPARTICLES ON BILAYER LIPID MEMBRANE PROPERTIES

2019 ◽  
Vol 53 (3 (250)) ◽  
pp. 208-213
Author(s):  
A.L. Torosyan ◽  
G.V. Ananyan ◽  
V.B. Arakelyan
Keyword(s):  
Zinc Oxide ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Membrane Properties ◽  
Oxide Nanoparticles ◽  
Electrical Field ◽  
Bilayer Lipid ◽  
The Stability

The effect of zinc oxide nanoparticles ($ \mathrm{ZnO} $) on the stability and conductivity of the bilayer lipid membrane (BLM) in solution was studied. It has been shown that $ \mathrm{ZnO} $ nanoparticles increase the stability of BLM in an electric field, and BLM becomes more stable with increasing their concentration. The increase in the stability of BLM in an electrical field is mainly due to the increase in the coefficient of linear tension of the pore edge, which is forming in BLM. It is also shown, that the presence of $ \mathrm{ZnO} $ nanoparticles in the solution surrounding BLM leads to a decrease in the BLM conductivity.

A Self-assembly Route for Double Bilayer Lipid Membrane Formation

ChemPhysChem ◽  
2010 ◽  
Vol 11 (3) ◽  
pp. 569-574 ◽  
Author(s):  
Xiaojun Han ◽  
Ammathnadu S. Achalkumar ◽  
Matthew R. Cheetham ◽  
Simon D. A. Connell ◽  
Benjamin R. G. Johnson ◽  
...  
Keyword(s):  
Self Assembly ◽  
Lipid Membrane ◽  
Bilayer Lipid Membrane ◽  
Membrane Formation ◽  
Bilayer Lipid

Polypyrrole Bridge as a Support for Alamethicin-Reconstituted Planar Bilayer Lipid Membranes

2011 ◽  
Author(s):  
Robert Northcutt ◽  
Vishnu-Baba Sundaresan ◽  
Sergio Salinas ◽  
Hao Zhang
Keyword(s):  
Ion Transport ◽  
Conducting Polymer ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Active Material ◽  
Experimental Studies ◽  
Bilayer Lipid Membrane ◽  
Electrical Impedance Spectroscopy ◽  
Bilayer Lipid ◽  
Ionic Components

Conducting polymer actuators and sensors utilize electrochemical reactions and associated ion transport at the polymer-electrolyte interface for their engineering function. Similarly, a bioderived active material utilizes ion transport through a protein and across a bilayer lipid membrane for sensing and actuation functions. Inspired by the similarity in ion transport process in a bilayer lipid membrane (BLM) and conducting polymers, we propose to build an integrated ionic device in which the ion transport through the protein in the bilayer lipid membrane regulates the electrolytic and mechanical properties of the conducting polymer. This article demonstrates the fabrication and characterization of a DPhPC planar BLM reconstituted with alamethicin and supported on a polypyrrole bridge measuring 100 μm × 500 μm and formed across micro-fabricated gold pads. The assembly is supported on silicon dioxide coated wafers and packaged into an electronic-ionic package for electrochemical characterization. The various ionic components in the integrated ionic device are characterized using electrical impedance spectroscopy (EIS), cyclic voltammetry (CV), and chronoamperometry (CA) measurements. The results from our experimental studies demonstrate the procedure to fabricate a rugged electro active polymer supported BLM that will serve as a platform for chemical, bioelectrical sensing and VOC detection.

Tethered-bilayer lipid membranes as a support for membrane-active peptides

2001 ◽  
Vol 29 (4) ◽  
pp. 613-617 ◽  
Author(s):  
B. A. Cornell ◽  
G. Krishna ◽  
P. D. Osman ◽  
R. D. Pace ◽  
L. Wieczorek
Keyword(s):  
Ion Channels ◽  
Present Article ◽  
Lipid Membranes ◽  
Gold Electrode ◽  
Lipid Membrane ◽  
Bilayer Lipid Membranes ◽  
Active Peptides ◽  
Bilayer Lipid ◽  
Membrane Active Peptides ◽  
Key Steps

An immunosensing device, comprising a lipid membrane incorporating ion channels tethered to the surface of a gold electrode, has been reported [Cornell, Braach-Maksvytis, King, Osman, Raguse, Wieczorek and Pace (1997) Nature (London) 387, 580–583]. The present article describes key steps in the assembly of the device and provides further evidence for its proposed sensing mechanism.

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