UV laser-induced fluorescence spectroscopy and laser Doppler flowmetry in the diagnostics of alopecia

2016 ◽  
Author(s):  
Diana P. Skomorokha ◽  
Yulia N. Pigoreva ◽  
Vladimir V. Salmin
Keyword(s):  
Fluorescence Spectroscopy ◽  
Laser Doppler Flowmetry ◽  
Laser Induced Fluorescence ◽  
Laser Doppler ◽  
Uv Laser ◽  
Doppler Flowmetry

scholarly journals Combined assessment of intestinal viability using laser doppler flowmetry and laser fluorescence spectroscopy

2021 ◽  
Vol 20 (2) ◽  
pp. 70-76
Author(s):  
A. A. Zacharenko ◽  
M. A. Belyaev ◽  
A. A. Trushin ◽  
D. A. Zaytcev ◽  
R. V. Kursenko ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Laser Doppler Flowmetry ◽  
Ischemia Reperfusion ◽  
Diagnostic Value ◽  
Laser Doppler ◽  
Laser Fluorescence ◽  
Doppler Flowmetry ◽  
Intraoperative Assessment ◽  
Before And After ◽  
Laser Fluorescence Spectroscopy

Introduction. The development of an objective non-invasive method for intraoperative assessment of intestinal viability remains urgent for modern surgery. In this context, the method of laser fluorescence spectroscopy (LFS) of coenzymes of oxidative metabolism, as well as a combination of this technique with the simultaneous use of laser Doppler flowmetry (LDF) seems promising. Materials and methods. The model of ischemia-reperfusion of the small intestine of 4 Californian Rabbits was used to study the relationship of the parameters of LFS and LDF with the histological picture. A model of intraoperative ischemia was used by temporarily clamping the trunk of the cranial mesenteric vascular bundle for 90 min, followed by intraoperative and postoperative reperfusion for 60 minutes and 24 hours, respectively. LDF and LFS data were recorded from intestine at the end of the intraoperative reperfusion period. 24 hours after the surgery, the animals were subjected to histologic evaluation of intestine ischemic changes, which were compared with the LDF and LFS data. Diagnostic value of LDF and LFS, and their combination for intraoperative assessment of intestinal viability were analyzed. Results. A significant correlation was found between the parameters of LDF, LFS and the degree of ischemic changes according to the histological data. The method of isolated assessment of the difference in the fluorescence of reduced nicotin adenine dinucleotide (NADH) before and after ischemia-reperfusion (77.3 %) has the greatest diagnostic value. The method has the highest sensitivity with a combination of differences in LDF and LFS values before and after ischemia-reperfusion (85.7 %) (P<0.05). Conclusions. LFS, as well as its combination with LDF, is a useful method for objective assessment of intestinal viability, which requires further research and has potential for clinical use.

The response of skin perfusion and of rheological and immunological variables to intravenous prostanoid administration in Raynaud’s phenomenon secondary to collagenosis

VASA ◽  
2005 ◽  
Vol 34 (4) ◽  
pp. 243-249 ◽  
Author(s):  
Drinda ◽  
Neumann ◽  
Pöhlmann ◽  
Vogelsang ◽  
Stein ◽  
...  
Keyword(s):  
Laser Doppler Flowmetry ◽  
Raynaud’S Phenomenon ◽  
Raynaud's Phenomenon ◽  
Plasma Viscosity ◽  
Laser Doppler ◽  
Erythrocyte Aggregation ◽  
Control Group ◽  
Long Term Effects ◽  
Doppler Flowmetry

Background: Prostanoids are used in the treatment of Raynaud’s phenomenon and acral perfusion disorders secondary to collagenosis. In subjective terms, intravenous administration of these agents produces success in more than 50% of patients. The therapeutic outcome of clinical administration of alprostadil or iloprost may vary from individual to individual. Patients and methods: The following variables were analysed in a cross-over study in 27 patients with collagenosis and Raynaud’s phenomenon: plasma viscosity and erythrocyte aggregation (rheological variables), partial pressure of oxygen and laser Doppler flowmetry in the finger region, and lymphocyte phenotyping and interleukin (IL) determinations (immunological variables). Results: Laser Doppler flowmetry revealed significant differences between patients with secondary Raynaud’s phenomenon and a control group of 25 healthy subjects. Laser Doppler readings did not change significantly as a result of the treatments. Therapy with iloprost produced a reduction in IL-1beta, L-selectin (CD 62 L) and IL-6. Conclusion: The change in immunological variables due to iloprost may explain the long-term effects of prostaglandins in the treatment of Raynaud’s phenomenon. From our results it is not possible to infer any preference for iloprost or alprostadil.

Microcirculation disorders in patients with vibration disease and comorbidity

2020 ◽  
pp. 672-672
Author(s):  
A. N. Kuks ◽  
N. V. Slivnitsyna
Keyword(s):  
Type 2 Diabetes ◽  
Laser Doppler Flowmetry ◽  
Laser Doppler ◽  
Combined Effects ◽  
Doppler Flowmetry ◽  
Vibration Disease ◽  
History Of

The results of laser Doppler flowmetry in patients with vibration disease associated with the combined effects of local and general vibration with a history of type 2 diabetes are presented.

Peculiarities of microcirculation in periodont tissues in children of key age groups sufficient type 1 diabetes. Part II

2019 ◽  
Vol 24 (2) ◽  
pp. 108-119 ◽  
Author(s):  
B. N. Davydov ◽  
D. A. Domenyuk ◽  
S. V. Dmitrienko
Keyword(s):  
Diabetes Mellitus ◽  
Blood Flow ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Laser Doppler Flowmetry ◽  
Laser Doppler ◽  
Diabetic Microangiopathy ◽  
Healthy Children ◽  
Doppler Flowmetry

Relevance. Morpho-functional changes in peripheral circulation established in type 1 diabetes mellitus correlate with changes in central hemodynamics, allowing the use of microcirculation indicators as diagnostic and prognostic criteria for assessing the degree of functional vascular disorders. Identifcation of microcirculation features of the blood by the method of laser Doppler flowmetry in children with different experience of type 1 diabetes in key age categories.Materials and methods. The study included 67 children with type 1 diabetes mellitus aged 12-15 years with an experience of the disease from six months to ten years. The comparison group consisted of 38 healthy children. The state of the microvasculature was assessed by laser Doppler flowmetry using a laser analyzer for capillary blood flow LAKK-OP.Results. In children with an experience of type 1 diabetes of less than two years, microcirculation disorders in periodontal tissues correspond to the hyperemic form, accompanied by increased perfusion, a decrease in the amplitude of low-frequency oscillations, increased heart rate, high blood flling, and blood flow bypass. For children with an endocrinopathy experience of more than three years, microcirculation disorders correspond to a stagnant form, combined with a decrease in perfusion due to stagnation of blood in the venular link, endothelial domination with suppression of neurogenic and cardiac fluctuations, low efciency and redistribution of blood flow in favor of the nutritive link.Conclusions. With the increase in experience, the degree of compensation of type 1 diabetes, the progression of diabetic microangiopathy, it is advisable to designate two stages of development of microcirculatory disorders. Early – compensatory with active adaptation, including neurogenic and endothelial regulation mechanisms. Late – decompensation with passive adaptation, supporting the effectiveness of microcirculation due to myogenic control of regulation, shunting and increasing the rate of blood outflow.

Laser doppler flowmetry in assessing the effectiveness of treatment of chronic demodex blefaritis

2017 ◽  
Vol 10 (2) ◽  
pp. 62-66 ◽  
Author(s):  
T.N. Safonova ◽  
◽  
N.P. Kintyukhina ◽  
V.V. Sidorov ◽  
O.V. Gladkova ◽  
...  
Keyword(s):  
Laser Doppler Flowmetry ◽  
Laser Doppler ◽  
Doppler Flowmetry
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