Microcirculation disorders in patients with vibration disease and comorbidity

Abstract Aim The study investigated changes in microvascular perfusion during post-exercise recovery in those with type 2 diabetes, with or without peripheral neuropathy, as well as in healthy controls and those with obesity. Methods Skin blood perfusion was assessed in each group using laser doppler flowmetry and laser speckle contrast imaging before and immediately after a six-minute walking test. Laser doppler flowmetry recordings underwent wavelet transformation to allow specific control mechanisms of blood perfusion to be studied (e.g. endothelial nitric oxide independent and dependent, neurogenic, myogenic, respiratory and cardiac mechanisms). Results Skin blood perfusion increased after exercise in all groups (22.3±28.1% with Laser speckle contrast imaging and 22.1±52.5% with laser doppler flowmetry). Throughout post-exercise recovery, the decrease was blunted in those with subclinical peripheral neuropathy and confirmed peripheral neuropathy when compared to the other three groups. After exercise, total spectral power increased in all groups. The relative contributions of each endothelial band was lower in those with confirmed peripheral neuropathy than in the healthy controls and those with obesity (nitric oxide-dependent function: 23.6±8.9% versus 35.5±5.8% and 29.3±8.8%, respectively; nitric oxide-independent function: 49.1±23.7% versus 53.3±10.4% and 64.6±11.4%, respectively). The neurogenic contribution decreased less in those with confirmed peripheral neuropathy and in those with type 2 diabetes alone, compared to those with subclinical peripheral neuropathy and those with obesity (-14.5±9.9% and -12.2±6.1% versus -26.5±4.7% and -21.7±9.4%, respectively). Conclusion Peripheral neuropathy, whatever the stage, altered the microvascular response to exercise via impaired endothelial and neurogenic mechanisms.

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METHOD FOR ANALYSIS OF THE DOPPLER-BROADENED SPECTRA OF LASER RADIATION FOR THE ASSESSMENT OF BLOOD MICROCIRCULATION DISTURBANCES IN DIABETES MELLITUS TYPE 2

Fundamental and Applied Problems of Engineering and Technology ◽

10.33979/2073-7408-2020-342-4-1-80-87 ◽

2020 ◽

Vol 4 (1) ◽

pp. 80-87

Author(s):

I.O. KOZLOV

Keyword(s):

Diabetes Mellitus ◽

Laser Radiation ◽

Laser Doppler Flowmetry ◽

Diabetes Mellitus Type 2 ◽

Feature Space ◽

Diabetes Mellitus Type ◽

Laser Doppler ◽

Doppler Flowmetry ◽

New Feature

The article is devoted to the development of laser Doppler flowmetry and analysis of the recorded signal to study the distribution of perfusion over the frequencies of Doppler broadening of laser radiation. The processing algorithm and the necessary technical conditions for the correct registration of the signal are shown. As examples of the proposed method implementation, the data are obtained from a healthy volunteer and a patient with diabetes mellitus type 2 and analyzed. According to the proposed method, processing of recorded data provides a new feature space for data analysis of laser Doppler flowmetry signal.

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Evaluation of Reflex Cold Provocation by Laser Doppler Flowmetry in Clinically Healthy Subjects with a History of Cold Hands

Angiology ◽

10.1177/000331979204300503 ◽

1992 ◽

Vol 43 (5) ◽

pp. 389-394 ◽

Cited By ~ 11

Author(s):

P. Gasser ◽

P. Müller ◽

D. Mauli ◽

C. Stäubli

Keyword(s):

Laser Doppler Flowmetry ◽

Healthy Subjects ◽

Laser Doppler ◽

Doppler Flowmetry ◽

History Of ◽

Cold Provocation

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NON-INVASIVE METHODS FOR STUDYING AGE-RELATED SKIN CHANGES IN VARIOUS MORPHOTYPES OF AGING

Успехи геронтологии ◽

10.34922/ae.2021.34.4.008 ◽

2021 ◽

pp. 558-565

Author(s):

Н.П. Теплюк ◽

С.В. Лебедева

Keyword(s):

Laser Doppler Flowmetry ◽

Ultrasound Examination ◽

Laser Doppler ◽

Doppler Flowmetry ◽

Skin Changes ◽

Non Invasive ◽

Age Related ◽

The Face ◽

Type 3

Цель работы - оценка изменений функционального состояния микроциркуляторного русла, изучение особенностей морфологических параметров кожи неинвазивными методами исследования при различных морфотипах старения. В исследовании приняли участие 55 человек 35-65 лет с инволюционными изменениями нижней трети лица. Выделены четыре морфотипа старения, в зависимости от которых пациенты были разделены на группы: 1-я - 19 пациенток с деформационным типом; 2-я - 17 пациенток с усталым; 3-я - 8 пациенток с мелкоморщинистым; 4-я - 11 пациенток со смешанным морфотипом. Для изучения состояния кожи использовали лазерную допплеровскую флоуметрию и УЗИ кожи (22 Mhz). Методом лазерной допплеровской флоуметрии выявлены достоверные изменения при сравнении показателей микроциркуляции и ультразвуковым методом исследования кожи - деформационного и мелкоморщинистого типов (p<0,05). У деформационного и смешанного типов наблюдали преимущественно атонический тип микроциркуляции (9,42±3,71 и 9,56±2,31 перф. ед. соответственно), а при мелкоморщинистом - спастический тип (7,86±1,6 перф. ед.), при усталом морфотипе показатель микроциркуляции был в пределах нормы (7,86±1,6 перф. ед.). Данные УЗИ кожи позволили выявить у пациенток с деформационным морфотипом старения наиболее высокие значения толщины эпидермиса (125,94±27,84 мкм), дермы (1 439±118,11 мкм), плотности дермы (12±2,59 у. е.) по сравнению с толщиной эпидермиса (85±22,01 мкм), толщиной дермы (1 130±68,55 мкм) и плотностью дермы (7,87±2,1 у. е.) при мелкоморщинистом морфотипе старения. The purpose of the work is to assess changes in the functional state of the microvasculature, study of the features of the morphological parameters of the skin by non-invasive research methods for various morphotypes of aging. The study involved 55 patients with involutional changes in the lower third of the face aged 35 to 65 years, included according with the inclusion and exclusion criteria. 4 morphotypes of aging were identified, depending on which patients were divided into corresponding groups: 1 - 19 patients with deformational type, 2 - 17 with tired type, 3 - 8 with fine wrinkled type, 4 - 11 with mixed morphotype of aging. To study the condition of the patients’ skin, laser Doppler flowmetry and ultrasound examination of the skin (22 Mhz) were used. The method of laser Doppler flowmetry revealed significant changes when comparing microcirculation indices and the ultrasound method for examining deformational and fine wrinkled type (p<0,05). In the deformational type and mixed type, a predominantly atonic type of microcirculation was observed (9,42±3,71 and 9,56±2,31 perf. u., respectively), and in fine wrinkled spastic type of microcirculation (7,86±1,6 perf. u.), in the oral morphotype the microcirculation index was within the normal range of 7,86±1,6 perf. u. The data of ultrasound examination of the skin made it possible to reveal in the deformational morphotype of aging high values of the thickness of the epidermis (125,94±27,84 μm), the dermis (1 439±118,11 μm) and the density of the dermis (12±2,59 a. u.) compared with the thickness of the epidermis 85±22,01 μm, the thickness of the dermis (1 130±68,55 μm) and the density of the dermis (7,87±2,1 c. u.) in the fine wrinkled morphotype of aging.

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The response of skin perfusion and of rheological and immunological variables to intravenous prostanoid administration in Raynaud’s phenomenon secondary to collagenosis

VASA ◽

10.1024/0301-1526.34.4.243 ◽

2005 ◽

Vol 34 (4) ◽

pp. 243-249 ◽

Cited By ~ 2

Author(s):

Drinda ◽

Neumann ◽

Pöhlmann ◽

Vogelsang ◽

Stein ◽

...

Keyword(s):

Laser Doppler Flowmetry ◽

Raynaud’S Phenomenon ◽

Raynaud's Phenomenon ◽

Plasma Viscosity ◽

Laser Doppler ◽

Erythrocyte Aggregation ◽

Control Group ◽

Long Term Effects ◽

Doppler Flowmetry

Background: Prostanoids are used in the treatment of Raynaud’s phenomenon and acral perfusion disorders secondary to collagenosis. In subjective terms, intravenous administration of these agents produces success in more than 50% of patients. The therapeutic outcome of clinical administration of alprostadil or iloprost may vary from individual to individual. Patients and methods: The following variables were analysed in a cross-over study in 27 patients with collagenosis and Raynaud’s phenomenon: plasma viscosity and erythrocyte aggregation (rheological variables), partial pressure of oxygen and laser Doppler flowmetry in the finger region, and lymphocyte phenotyping and interleukin (IL) determinations (immunological variables). Results: Laser Doppler flowmetry revealed significant differences between patients with secondary Raynaud’s phenomenon and a control group of 25 healthy subjects. Laser Doppler readings did not change significantly as a result of the treatments. Therapy with iloprost produced a reduction in IL-1beta, L-selectin (CD 62 L) and IL-6. Conclusion: The change in immunological variables due to iloprost may explain the long-term effects of prostaglandins in the treatment of Raynaud’s phenomenon. From our results it is not possible to infer any preference for iloprost or alprostadil.

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