Real-time measurement methods of selected physical parameters of human tissues using optical fiber probe

2000 ◽  
Author(s):  
Andrzej Zajac ◽  
Zdzislaw Jankiewicz
2003 ◽  
Vol 57 (2) ◽  
pp. 132-138 ◽  
Author(s):  
E. Tamburini ◽  
G. Vaccari ◽  
S. Tosi ◽  
A. Trilli

Near-infrared (NIR) spectroscopy has been developed as a noninvasive tool for the direct, real-time monitoring of glucose, lactic acid, acetic acid, and biomass in liquid cultures of microrganisms of the genera Lactobacillus and Staphylococcus. This was achieved employing a steam-sterilizable optical-fiber probe immersed in the culture (In-line Interactance System®). Second-derivative spectra obtained were subjected to partial least-squares (PLS) regression and the results were used to build predictive models for each analyte of interest. Multivariate regression was carried out on two different sets of spectra, namely whole broth minus the spectral subtraction of water, and raw spectra. A comparison of the two models showed that the first cannot be properly applied to real-time monitoring, so this work suggests calibration based on non-difference spectra, demonstrating it to be sufficiently reliable to allow the selective determination of the analytes with satisfactory levels of prediction (standard error of prediction (SEP) < 10%). Direct interfacing of the NIR system to the bioreactor control system allowed the implementation of completely automated monitoring of different cultivation strategies (continuous, repeated batch). The validity of the in-line analyses carried out was found to depend crucially on maintaining constant hydrodynamic conditions of the stirred cultures because both gas flow and stirring speed variations were found to markedly influence the spectral signal.


2020 ◽  
Vol 321 ◽  
pp. 128469 ◽  
Author(s):  
Jingxian Yu ◽  
Peipei Jia ◽  
Shengping Wang ◽  
Heike Ebendorff-Heidepriem ◽  
Andrew D. Abell

2016 ◽  
Vol 21 (12) ◽  
pp. 127008 ◽  
Author(s):  
Luke A. Parkinson ◽  
Caroline E. Gargett ◽  
Natharnia Young ◽  
Anna Rosamilia ◽  
Aditya V. Vashi ◽  
...  

Author(s):  
Kodai Hanyu ◽  
Takayuki Saito

Gas-liquid two-phase flows are widely encountered in industrial plants such as chemical reactors, power plants, environmental plants and so on. It is essential to clarify the dissolution process of the bubbles and the structure of the gas-liquid two-phase flows for realizing the efficient operation of these industrial reactors. However, it is very difficult to clarify them because of their complexity. We made a challenge of clarifying them in detail. First, we discuss the dynamical processes of the mass transfer from a zigzagging CO2 bubble of 2.9 mm in equivalent diameter by using three measurement methods (i.e. LIF (laser induced fluorescence)/HPTS (8-hydroxypyrene-1, 3, 6-trislfonic acid), PIV and a newly developed photoelectric optical fiber probe: POFP) effectively and mutually-complementarily. We directly visualized the dynamical mass transfer process from a zigzagging CO2 bubble to the surrounding liquid by using LIF/HPTS. We measured the surrounding liquid motion induced by the bubble buoyancy using PIV. We visualized a high-CO2-concentration thin layer around the bubble was transported to the bubble rear and accumulated into the horseshoe-like vortices. The clear horse-shoe-like vortices were observed just after the launch of the bubble from a needle. Then, the wreckage of the bubble wake was transported widely into the surrounding liquid by the buoyancy driven flows. We measured directly the CO2 concentration profile inside the bubble wake by using POFP. We succeeded in clarifying the profile of the CO2 concentration inside the bubble wake, which is difficult to obtain from only the LIF/HPTS method. From this result, we obtained that the CO2 concentration takes the maximum at the center region of the bubble wake and sharply decreases toward the outer edge of the bubble wake. Second, we simultaneously measured the diameters and velocities of the bubble by using POFP. We confirmed the performance of the POFP; the results via POFP were compared with those obtained from the visualization of the bubbles by using a high-speed video camera. We demonstrated mutually-complementary use of three measurement methods is very effective to experimentally understand the dynamical processes of the mass transfer from a zigzagging CO2 bubble to the surrounding liquid.


2016 ◽  
Author(s):  
Huajie Han ◽  
Yonggang Gu ◽  
Guocheng Liu ◽  
Chao Zhai

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