In-vivo detection of tumor-infiltrated axillary lymph nodes with a handheld beta-sensitive probe: a phantom study

2001 ◽  
Author(s):  
Raymond R. Raylman ◽  
Jamal Derakhshan
Keyword(s):  
Lymph Nodes ◽  
Phantom Study ◽  
Axillary Lymph Nodes ◽  
Axillary Lymph ◽  
In Vivo Detection

A CALIBRATION PHANTOM FOR DIRECT, IN VIVO MEASUREMENT OF 241Am IN THE AXILLARY LYMPH NODES

2009 ◽  
Vol 97 (3) ◽  
pp. 219-227 ◽  
Author(s):  
Rachel Zeman ◽  
Megan Lobaugh ◽  
Henry Spitz ◽  
Samuel Glover ◽  
David Hickman
Keyword(s):  
Lymph Nodes ◽  
Axillary Lymph Nodes ◽  
Axillary Lymph ◽  
In Vivo Measurement ◽  
Calibration Phantom

scholarly journals Highly Sensitive Identification of Lymphatic and Hematogenous Metastasis Routes of Novel Radiolabeled Exosomes Using Non-invasive PET Imaging

2020 ◽  
Author(s):  
Kyung Oh Jung ◽  
Young-Hwa Kim ◽  
Seock-Jin Chung ◽  
Keon Wook Kang ◽  
Siyeon Rhee ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Optical Imaging ◽  
Pet Imaging ◽  
Ex Vivo ◽  
The Body ◽  
Hematogenous Metastasis ◽  
Axillary Lymph Nodes ◽  
Axillary Lymph ◽  
Gamma Counter

Clinically, there has been significant interest in the use of exosomes for diagnostic applications as promising biomarkers and therapeutic applications as therapeutic vehicles. However, knowledge of in vivo physiological biodistribution of exosomes was difficult to assess until now. Physiological distribution of exosomes in the body must be elucidated for clinical application. In this study, we aimed to develop reliable and novel methods to monitor biodistribution of exosomes using in vivo PET and optical imaging.MethodsExosomes were isolated from cultured medium of 4T1, mouse breast cancer cells. Exosomes were labeled with Cy7 and 64Cu (or 68Ga). In mice, radio/fluorescent dye-labeled exosomes were injected through the lymphatic routes (footpad injection) and hematogenous metastatic routes (tail vein injection). Fluorescence and PET images were obtained and quantified. Radio-activity of ex vivo organs was measured by gamma counter.ResultsPET signals from exosomes in the lymphatic metastatic route were observed in the draining lymph nodes, which are not distinguishable with optical imaging. Immunohistochemistry revealed greater uptake of exosomes in brachial and axillary lymph nodes than inguinal lymph node. After administration through the hematogenous metastasis pathway, accumulation of exosomes was clearly observed in PET images in the lungs, liver, and spleen, showing results similar to ex vivo gamma counter data.ConclusionExosomes from tumor cells were successfully labeled with 64Cu (or 68Ga) and visualized by PET imaging. These results suggest that this cell type-independent, quick, and easy exosome labeling method using PET isotopes could provide valuable information for further application of exosomes in the clinic.

scholarly journals The Value of T2* in Differentiating Metastatic from Benign Axillary Lymph Nodes in Patients with Breast Cancer - A Preliminary In Vivo Study

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e84038 ◽  
Author(s):  
Chuanming Li ◽  
Shan Meng ◽  
Xinhua Yang ◽  
Jian Wang ◽  
Jiani Hu
Keyword(s):  
Breast Cancer ◽  
Lymph Nodes ◽  
In Vivo Study ◽  
Axillary Lymph Nodes ◽  
Axillary Lymph

Adjuvant chemotherapy for women with breast cancer with 10 or more positive axillary lymph nodes—will less mean more?

2006 ◽  
Vol 6 (3-4) ◽  
pp. 153-156
Author(s):  
Aswini Padmanabhan ◽  
Agustin A. Garcia
Keyword(s):  
Breast Cancer ◽  
Adjuvant Chemotherapy ◽  
Lymph Nodes ◽  
Axillary Lymph Nodes ◽  
Axillary Lymph

An Unusual Metastasis of Posterior Neck and Axillary Lymph Nodes from Nasopharyngeal Carcinoma

2016 ◽  
Vol 32 (2) ◽  
pp. 23-27
Author(s):  
Yong Tae Hong ◽  
◽  
Phan Huu Ngoc Minh ◽  
Ki Hwan Hong ◽  
◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Lymph Nodes ◽  
Axillary Lymph Nodes ◽  
Axillary Lymph ◽  
Posterior Neck

Clinical Significance of Microscopic Metastases in Axillary Lymph Nodes in Primary Operable Breast Cancer. (Literature Review)

2018 ◽  
Vol 15 (1) ◽  
pp. 116-123
Author(s):  
V.E. Ponomarev ◽  
◽  
J.V. Vishnevskaja ◽  
N.I. Mehtieva ◽  
S.B. Polikarpova ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Literature Review ◽  
Lymph Nodes ◽  
Clinical Significance ◽  
Operable Breast Cancer ◽  
Axillary Lymph Nodes ◽  
Axillary Lymph ◽  
Primary Operable Breast Cancer

scholarly journals New insights in the expression of stromal caveolin 1 in breast cancer spread to axillary lymph nodes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Cristian Scatena ◽  
Giovanni Fanelli ◽  
Giuseppe Nicolò Fanelli ◽  
Michele Menicagli ◽  
Paolo Aretini ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Cancer Metastasis ◽  
Tumor Stroma ◽  
Disease Recurrence ◽  
Metastatic Spread ◽  
Axillary Lymph Nodes ◽  
Axillary Lymph ◽  
Axillary Lymph Node Metastases ◽  
Stromal Compartment ◽  
Metastatic Sites

AbstractRecent evidence suggests that a loss of expression of caveolin in the stromal compartment (sCav-1) of human invasive breast carcinoma (IBC) may be a predictor of disease recurrence, metastasis and poor outcome. At present, there is little knowledge regarding the expression of sCav-1 at the metastatic sites. We therefore studied sCav-1 expression in IBCs and in their axillary lymph nodes to seek a correlation with cancer metastasis. 189 consecutive invasive IBCs (53 with axillary lymph node metastases and 136 without) were studied by immunohistochemistry, using a rabbit polyclonal anti-Cav-1 antibody. In IBCs sCav-1 was evaluated in fibroblasts scattered in the tumor stroma whereas in lymph nodes sCav-1 was assessed in fibroblast-like stromal cells. For the first time, we observed a statistically significant progressive loss of sCav-1 from normal/reactive axillary lymph nodes of tumors limited to the breast to metastatic axillary lymph nodes, through normal/reactive axillary lymph nodes of tumors with axillary metastatic spread. These data indicate that Cav-1 expressed by the stromal compartment of lymph nodes, somehow, may possibly contribute to metastatic spread in IBC.

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