The focus of this work is to study surface effects on the friction factor a magneto-rheological (MR) fluid flowing through a grooved channel under various magnetic fields and volumetric flow rates. Based on the experimental data, a relation is developed for the friction factor of MR fluid in channel flow in terms of Mason number evaluated at the surface, and the depth of the grooves. Using this relation, the pressure loss of a MR fluid flowing through a channel with grooved walls can be determined without implementing a constitutive model for MR fluids or utilizing the concept of shear yield stress. Several grooves with different configurations in channel walls have been considered. From the experimental results it has been demonstrated that under an applied magnetic field, the grooved surface would increase the friction factor of MR fluid flow significantly when comparing to the surface without grooving. The depth of grooves plays an important role in this increment.