<title>Polarization phase reconstruction of biological tissue architectonics: Part 2. Study of polarizing intercorrelative function of coherent images of phase-inhomogeneous layer anisotropy</title>

Microprobe analysis of biological tissue is now in the end phase of transition from instrumental and technique development to applications pertinent to questions of physiological relevance. The promise,implicit in early investigative efforts, is being fulfilled to an extent much greater than many had predicted. It would thus seem appropriate to briefly report studies exemplifying this, ∿. In general, the distributions of ions in tissue in a preselected physiological state produced by variations in the external environment is of importance in elucidating the mechanisms of exchange and regulation of these ions.

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Complementary Z-Contrast Imaging and Digital Image Processing

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100118394 ◽

1985 ◽

Vol 43 ◽

pp. 304-305

Author(s):

K. N. Colonna ◽

G. Oliphant

Keyword(s):

Image Processing ◽

Heavy Metal ◽

Digital Image Processing ◽

Digital Image ◽

Biological Tissue ◽

Biological Imaging ◽

Tissue Preparation ◽

Contrast Imaging ◽

Imaging Tool ◽

Z Contrast

Harmonious use of Z-contrast imaging and digital image processing as an analytical imaging tool was developed and demonstrated in studying the elemental constitution of human and maturing rabbit spermatozoa. Due to its analog origin (Fig. 1), the Z-contrast image offers information unique to the science of biological imaging. Despite the information and distinct advantages it offers, the potential of Z-contrast imaging is extremely limited without the application of techniques of digital image processing. For the first time in biological imaging, this study demonstrates the tremendous potential involved in the complementary use of Z-contrast imaging and digital image processing.Imaging in the Z-contrast mode is powerful for three distinct reasons, the first of which involves tissue preparation. It affords biologists the opportunity to visualize biological tissue without the use of heavy metal fixatives and stains. For years biologists have used heavy metal components to compensate for the limited electron scattering properties of biological tissue.

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An Absolute Phase Estimation Method for Interferometry Imagery

Journal of Engineering Technology and Applied Physics ◽

10.33093/jetap.2019.1.2.4 ◽

2019 ◽

Vol 1 (2) ◽

pp. 14-19

Author(s):

Sui Ping Lee ◽

Yee Kit Chan ◽

Tien Sze Lim

Keyword(s):

Noise Suppression ◽

Estimation Method ◽

Phase Image ◽

Phase Estimation ◽

Phase Reconstruction ◽

Estimation Algorithms ◽

Absolute Phase ◽

Filtering Rate ◽

Interferometric Phase ◽

Image Performance

Accurate interpretation of interferometric image requires an extremely challenging task based on actual phase reconstruction for incomplete noise observation. In spite of the establishment of comprehensive solutions, until now, a guaranteed means of solution method is yet to exist. The initially observed interferometric image is formed by 2π-periodic phase image that wrapped within (-π, π]. Such inverse problem is further corrupted by noise distortion and leads to the degradation of interferometric image. In order to overcome this, an effective algorithm that enables noise suppression and absolute phase reconstruction of interferometric phase image is proposed. The proposed method incorporates an improved order statistical filter that is able to adjust or vary on its filtering rate by adapting to phase noise level of relevant interferometric image. Performance of proposed method is evaluated and compared with other existing phase estimation algorithms. The comparison is based on a series of computer simulated and real interferometric data images. The experiment results illustrate the effectiveness and competency of the proposed method.

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