The development of skin immersion clearing method for increasing of laser exposure efficiency on subcutaneous objects

Author(s):  
Alexandra M. Kozina ◽  
Elina A. Genina ◽  
Georgy S. Terentyuk ◽  
Artem G. Terentyuk ◽  
Alexey N. Bashkatov ◽  
...  
Author(s):  
Burton B. Silver ◽  
Theodore Lawwill

Dutch-belted 1 to 2.5 kg anesthetized rabbits were exposed to either xenon or argon laser light administered in a broad band, designed to cover large areas of the retina. For laser exposure, the pupil was dilated with atropine sulfate 1% and pheny lephrine 10%. All of the laser generated power was within a band centered at 5145.0 Anstroms. Established threshold for 4 hour exposures to laser irradiation are in the order of 25-35 microwatts/cm2. Animals examined for ultrastructural changes received 4 hour threshold doses. These animals exhibited ERG, opthalmascopic, and histological changes consistent with threshold damage.One month following exposure the rabbits were killed with pentobarbitol. The eyes were immediately enucleated and dissected while bathed in 3% phosphate buffered gluteraldehyde.


Author(s):  
JS Deitch ◽  
KL Smith ◽  
JW Swann ◽  
JN Turner

Neurons labeled with horseradish peroxidase and reacted with diaminobenzidine (DAB) can be imaged using a confocal scanning laser microscope (CSLM) in the reflection mode. In contrast to fluorescent markers, the DAB reaction product is thought to be stable and can be observed by both light and electron microscopy. We have investigated the sensitivity of the DAB reaction product to laser irradiation, and present the spectrophotometric properties of DAB before and after exposure in the CSLM.Pyramidal neurons in slices of rat hippocampus were injected with biocytin (a biotin-lysine complex), fixed overnight in 4% paraformaldehyde, and vibratome sectioned at 75 μm. Biocytin was detected with avidin-HRP (1:200) in 0.5% Triton X-100, incubated in DAB (0.5 mg/ml) with or without 0.04% nickel ammonium sulfate (Ni), dehydrated, and imaged in a Bio Rad MRC-500 CSLM with an argon ion laser (488 and 514 nm). Spectrophotometric measurements of the soma were made on a Zeiss microspectrophotometer, as a function of laser exposure (100-1000 scans) and staining protocol.


Author(s):  
Vincenzo De Michele ◽  
Emmanuel Marin ◽  
Aziz Boukenter ◽  
Marco Cannas ◽  
Sylvain Girard ◽  
...  

ACS Omega ◽  
2020 ◽  
Vol 5 (32) ◽  
pp. 20543-20547
Author(s):  
Bo Wang ◽  
Sisi Yang ◽  
Yu Wang ◽  
Younghee Kim ◽  
Han Htoon ◽  
...  

2017 ◽  
Vol 19 (4) ◽  
pp. 227-231 ◽  
Author(s):  
Luciana Guedes de Almeida ◽  
Luiz Philippe da Silva Sergio ◽  
Flavia de Paoli ◽  
Andre Luiz Mencalha ◽  
Adenilson de Souza da Fonseca

2015 ◽  
Vol 59 (2) ◽  
pp. 205011-205017 ◽  
Author(s):  
Akira Sakamoto ◽  
Manabu Numata ◽  
Yasuhiro Ogasawara ◽  
Mami Hatanaka ◽  
Yukari Motosugi ◽  
...  

Author(s):  
Shaojun Huang ◽  
Yuming Zhao ◽  
Konstantin Filonenko ◽  
Yun Wang ◽  
Tianlong Xiong ◽  
...  

Retina ◽  
2014 ◽  
Vol 34 (12) ◽  
pp. 2388-2396 ◽  
Author(s):  
Gregory D. Lee ◽  
Caroline R. Baumal ◽  
David Lally ◽  
John D. Pitcher ◽  
James Vander ◽  
...  

2013 ◽  
Vol 84 (3) ◽  
pp. 246-251 ◽  
Author(s):  
Stephanie E. Waggel ◽  
Ewan J. Hutchison

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