Protein Kinase C-Mediated Down-Regulation of β1-Adrenergic Receptor Gene Expression in Rat C6 Glioma Cells

1998 ◽  
Vol 54 (1) ◽  
pp. 14-21 ◽  
Author(s):  
Zhongwei Li ◽  
Vidita A. Vaidya ◽  
John D. Alvaro ◽  
Philip A. Iredale ◽  
Richard Hsu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adrenergic Receptor ◽  
Receptor Gene ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Kinase C ◽  
Receptor Gene Expression ◽  
Adrenergic Receptor Gene ◽  
Rat C6 Glioma

Adrenergic Regulation of ICER (Inducible Cyclic AMP Early Repressor) and β1-Adrenergic Receptor Gene Expression in C6 Glioma Cells

2002 ◽  
Vol 67 (2) ◽  
pp. 490-497 ◽  
Author(s):  
Laura Rydelek Fitzgerald ◽  
Zhongwei Li ◽  
Curtis A. Machida ◽  
Peter H. Fishman ◽  
Ronald S. Duman
Keyword(s):  
Gene Expression ◽  
Cyclic Amp ◽  
Adrenergic Receptor ◽  
Receptor Gene ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Adrenergic Regulation ◽  
Receptor Gene Expression ◽  
Adrenergic Receptor Gene

scholarly journals Differential Regulation of IGF-Binding Protein Gene Expression by cAMP in Rat C6 Glioma Cells

Endocrinology ◽  
2001 ◽  
Vol 142 (9) ◽  
pp. 3917-3925 ◽  
Author(s):  
Lai Wang ◽  
Xiuye Ma ◽  
Lee-Chuan C. Yeh ◽  
Martin L. Adamo
Keyword(s):  
Gene Expression ◽  
Protein Gene ◽  
Glioma Cells ◽  
Differential Regulation ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
Igf Binding ◽  
Binding Protein Gene ◽  
Igf Binding Protein ◽  
Rat C6 Glioma

scholarly journals Altered Beta-2 Adrenergic Receptor Gene Expression in Human Clinical Hypertension

2009 ◽  
Vol 11 (1) ◽  
pp. 17-26 ◽  
Author(s):  
Jennifer R. Dungan ◽  
Yvette P. Conley ◽  
Taimour Y. Langaee ◽  
Julie A. Johnson ◽  
Shawn M. Kneipp ◽  
...  
Keyword(s):  
Gene Expression ◽  
Coronary Artery ◽  
Coronary Artery Bypass ◽  
Adrenergic Receptor ◽  
Artery Bypass ◽  
Receptor Gene ◽  
Positive Family History ◽  
Beta 2 ◽  
Receptor Gene Expression ◽  
Adrenergic Receptor Gene

Objectives: The beta-2 adrenergic receptor is involved in mediating vasodilatation via neurohumoral and sympathetic nervous system pathways. Alterations in beta-2 adrenergic receptor gene expression (mRNA transcription) may contribute to the hypertensive phenotype. Human gene expression in clinical phenotypes remains largely unexplored due to ethical constraints involved in obtaining human tissue. We devised a method to obtain normally discarded internal mammary artery tissue from coronary artery bypass graft patients. We then investigated differences in hypertensive and normotensive participants' beta-2 adrenergic receptor gene expression in this tissue. Methods: We collected arterial tissue samples from 46 coronary artery bypass patients in a surgical setting. Using 41 of the samples, we performed TaqMan real-time polymerase chain reaction (RT-PCR) and used the delta delta cycle threshold (ΔΔCt) relative quantitation method for determination of fold-differences in gene expression between normotensive and hypertensive participants. The beta-2 adrenergic receptor target was normalized to glyceraldehyde-phosphate dehydrogenase. Results: Participants with hypertension had significantly less-expressed beta-2 adrenoceptor gene (2.76-fold, p < .05) compared to normotensive participants. After Bonferroni correction, gene expression did not differ by race, gender, type/dose of β-blocker prescribed, positive family history of hypertension, or diagnosis of diabetes mellitus type 2. Conclusions: These data support the possibility of a molecular basis for impaired adrenoceptor-mediated vascular tone in hypertension. Modification and extension of this research is required.

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