scholarly journals SKP1 drives the prophase I to metaphase I transition during male meiosis

2020 ◽  
Vol 6 (13) ◽  
pp. eaaz2129 ◽  
Author(s):  
Yongjuan Guan ◽  
N. Adrian Leu ◽  
Jun Ma ◽  
Lukáš Chmátal ◽  
Gordon Ruthel ◽  
...  
Keyword(s):  
Okadaic Acid ◽  
Meiotic Prophase ◽  
Essential Role ◽  
Male Meiosis ◽  
Prophase I ◽  
Ubiquitin E3 Ligase ◽  
Competence Factor ◽  
Competence Acquisition ◽  
Mi Transition ◽  
Metaphase I

The meiotic prophase I to metaphase I (PI/MI) transition requires chromosome desynapsis and metaphase competence acquisition. However, control of these major meiotic events is poorly understood. Here, we identify an essential role for SKP1, a core subunit of the SKP1–Cullin–F-box (SCF) ubiquitin E3 ligase, in the PI/MI transition. SKP1 localizes to synapsed chromosome axes and evicts HORMAD proteins from these regions in meiotic spermatocytes. SKP1-deficient spermatocytes display premature desynapsis, precocious pachytene exit, loss of PLK1 and BUB1 at centromeres, but persistence of HORMAD, γH2AX, RPA2, and MLH1 in diplonema. Strikingly, SKP1-deficient spermatocytes show sharply reduced MPF activity and fail to enter MI despite treatment with okadaic acid. SKP1-deficient oocytes exhibit desynapsis, chromosome misalignment, and progressive postnatal loss. Therefore, SKP1 maintains synapsis in meiosis of both sexes. Furthermore, our results support a model where SKP1 functions as the long-sought intrinsic metaphase competence factor to orchestrate MI entry during male meiosis.

scholarly journals Regulation of the meiotic prophase I to metaphase I transition in mouse spermatocytes

Chromosoma ◽  
2008 ◽  
Vol 117 (5) ◽  
pp. 471-485 ◽  
Author(s):  
Fengyun Sun ◽  
Mary Ann Handel
Keyword(s):  
Meiotic Prophase ◽  
Prophase I ◽  
Meiotic Prophase I ◽  
Metaphase I

scholarly journals YTHDC2 Is Essential for Pachytene Progression and Prevents Aberrant Microtubule-Driven Telomere Clustering in Male Meiosis

2021 ◽  
Author(s):  
Rong Liu ◽  
Seth D. Kasowitz ◽  
David Homolka ◽  
N. Adrian Leu ◽  
Jordan T. Shaked ◽  
...  
Keyword(s):  
Germ Cells ◽  
Essential Role ◽  
Cell Types ◽  
Male Meiosis ◽  
Pachytene Stage ◽  
Meiotic Cell ◽  
Microtubule Network ◽  
Meiotic Progression ◽  
Prophase I ◽  
Microtubule Polymerization

SUMMARYMechanisms driving the prolonged meiotic prophase I are poorly understood. The RNA helicase YTHDC2 is critical for mitosis to meiosis transition, as YTHDC2-deficient mouse germ cells initiate meiosis but arrest with mixed characteristics of mitotic and meiotic cell types. However, YTHDC2 is also highly expressed in normal pachytene cells. Here we identify an essential role for YTHDC2 in meiotic progression. Specifically, we find that YTHDC2 deficiency causes microtubule-dependent telomere clustering and apoptosis at the pachytene stage of prophase I, and thus a failure to advance to the diplotene stage. Depletion of YTHDC2 results in a massively dysregulated transcriptome in pachytene cells, with a tendency toward upregulation of genes normally expressed in mitotic germ cells and downregulation of meiotic transcripts. Dysregulation does not correlate with the m6A status of RNAs and YTHDC2-bound mRNAs are enriched in genes upregulated in mutant germ cells, revealing that YTHDC2 primarily targets its substrate mRNAs for degradation. Finally, altered transcripts in YTHDC2-deficient pachytene cells encode microtubule network proteins and inhibition of microtubule polymerization disperses clustered telomeres. Together, our results demonstrate that YTHDC2 regulates the prolonged pachytene stage of prophase I by perpetuating a meiotic transcriptome and preventing changes in the microtubule network that could lead to aberrant telomere clustering.

scholarly journals The CSN/COP9 Signalosome Regulates Synaptonemal Complex Assembly during Meiotic Prophase I of Caenorhabditis elegans

PLoS Genetics ◽  
2014 ◽  
Vol 10 (11) ◽  
pp. e1004757 ◽  
Author(s):  
Heather Brockway ◽  
Nathan Balukoff ◽  
Martha Dean ◽  
Benjamin Alleva ◽  
Sarit Smolikove
Keyword(s):  
Caenorhabditis Elegans ◽  
Synaptonemal Complex ◽  
Meiotic Prophase ◽  
Cop9 Signalosome ◽  
Complex Assembly ◽  
Prophase I ◽  
Meiotic Prophase I

Cohesin component dynamics during meiotic prophase I in mammalian oocytes

2004 ◽  
Vol 12 (3) ◽  
pp. 197-213 ◽  
Author(s):  
Ignacio Prieto ◽  
Charles Tease ◽  
Nieves Pezzi ◽  
José M. Buesa ◽  
Sagrario Ortega ◽  
...  
Keyword(s):  
Meiotic Prophase ◽  
Prophase I ◽  
Meiotic Prophase I

scholarly journals Nuclear Envelope-Associated Chromosome Dynamics during Meiotic Prophase I

2018 ◽  
Vol 5 ◽  
Author(s):  
Xinhua Zeng ◽  
Keqi Li ◽  
Rong Yuan ◽  
Hongfei Gao ◽  
Junling Luo ◽  
...  
Keyword(s):  
Nuclear Envelope ◽  
Meiotic Prophase ◽  
Chromosome Dynamics ◽  
Prophase I ◽  
Meiotic Prophase I

scholarly journals A mutation in the endonuclease domain of mouse MLH3 reveals novel roles for MutLγ during crossover formation in meiotic prophase I

PLoS Genetics ◽  
2019 ◽  
Vol 15 (6) ◽  
pp. e1008177 ◽  
Author(s):  
Melissa Toledo ◽  
Xianfei Sun ◽  
Miguel A. Brieño-Enríquez ◽  
Vandana Raghavan ◽  
Stephen Gray ◽  
...  
Keyword(s):  
Meiotic Prophase ◽  
Prophase I ◽  
Meiotic Prophase I ◽  
Endonuclease Domain

Chromosome pairing and potential for intergeneric recombination in some hypotetraploid somatic hybrids of Lycopersicon esculentum (+) Solanum tuberosum

Genome ◽  
1993 ◽  
Vol 36 (6) ◽  
pp. 1032-1041 ◽  
Author(s):  
J. H. de Jong ◽  
A. M. A. Wolters ◽  
J. M. Kok ◽  
H. Verhaar ◽  
J. van Eden
Keyword(s):  
Solanum Tuberosum ◽  
Lycopersicon Esculentum ◽  
Synaptonemal Complex ◽  
Chromosome Pairing ◽  
Somatic Hybrids ◽  
Cytogenetic Study ◽  
Unreduced Gametes ◽  
Root Tip ◽  
Prophase I ◽  
Metaphase I

Three somatic hybrids resulting from protoplast fusions of a diploid kanamycin-resistant line of tomato (Lycopersicon esculentum) and a dihaploid hygromycin-resistant transformant of a monohaploid potato (Solanum tuberosum) line were used for a cytogenetic study on chromosome pairing and meiotic recombination. Chromosome counts in root-tip meristem cells revealed two hypotetraploids with chromosome complements of 2n = 46 and one with 2n = 47. Electron microscope analyses of synaptonemal complex spreads of hypotonically burst protoplasts at mid prophase I showed abundant exchanges of pairing partners in multivalents involving as many as eight chromosomes. In the cells at late pachytene recombination nodules were found in multivalents on both sides of pairing partner exchanges, indicating recombination at both homologous and homoeologous sites. Light microscope observations of pollen mother cells at late diakinesis and metaphase I also revealed multivalents, though their occurrence in low frequencies betrays the reduction of multivalent number and complexity. Precocious separation of half bivalents at metaphase I and lagging of univalents at anaphase I were observed frequently. Bridges, which may result from an apparent inversion loop found in the synaptonemal complexes of a mid prophase I nucleus, were also quite common at anaphase I, though the expected accompanying fragments could be detected in only a few cells. Most striking were the high frequencies of first division restitution in preparations at metaphase II/anaphase II, giving rise to unreduced gametes. In spite of the expected high numbers of balanced haploid and diploid gametes, male fertility, as revealed by pollen staining, was found to be negligible.Key words: synaptonemal complex, recombination, chromosome pairing, somatic hybrid, Lycopersicon esculentum (+) Solanum tuberosum.

Persistence of two Y chromosomes through meiotic prophase and metaphase I in an XYY man

1991 ◽  
Vol 87 (4) ◽  
Author(s):  
R.M. Speed ◽  
M.J.W. Faed ◽  
P.J. Batstone ◽  
K. Baxby ◽  
W. Barnetson
Keyword(s):  
Meiotic Prophase ◽  
Y Chromosomes ◽  
Metaphase I
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