BIOTIN (BIOS IIB, VITAMIN H)--AN ESSENTIAL GROWTH FACTOR FOR CERTAIN STAPHYLOCOCCI

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a well-known environmental teratogenic agent for cleft palate. But transforming growth factor β3 (TGF-β3) is an essential growth factor for palatogenesis. This study is to clarify effects of TCDD and TGF-β3 in mouse embryonic palatal mesenchymal (MEPM) cells. The result showed that with increase of TCDD (0.5 nM-10 nM), the expression of TGF-β3 increased, but after 10 nM TCDD, the expression of TGF-β3 reduced. The viabilities of MEPM cells decreased in 10 nM TCDD-treated group. But the viabilities increased in 10 ng/mL TGF-β3-treated group, or the viabilities were between that of them in combination of 10 nM TCDD and 10 ng/mL TGF-β3-treated group. This phenomenon was the same as the motilities. In addition, we found that the expression of phosphorylated Smad2/3 and Smad7 was increased by 10 nM TCDD, 10 ng/mL TGF-β3, or combination of 10 nM TCDD and 10 ng/mL TGF-β3 induced, but the expression of Smad4 was decreased. These data revealed that the TGF-β/Smad signaling pathway affected TCDD and TGF-β3 in MEPM cells.

Download Full-text

Identification of lactoferrin as an essential growth factor for human lymphocytic cell lines in serum-free medium

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research ◽

10.1016/0167-4889(83)90099-x ◽

1983 ◽

Vol 763 (4) ◽

pp. 377-382 ◽

Cited By ~ 112

Author(s):

Shuichi Hashizume ◽

Kazuhiko Kuroda ◽

Hiroki Murakami

Keyword(s):

Growth Factor ◽

Cell Lines ◽

Serum Free Medium ◽

Free Medium ◽

Serum Free ◽

Lymphocytic Cell ◽

Essential Growth ◽

Essential Growth Factor

Download Full-text

Plasma-Rich in Growth Factor and its Clinical Application

Current Stem Cell Research & Therapy ◽

10.2174/1574888x16666210120101600 ◽

2021 ◽

Vol 16 ◽

Author(s):

Mohsen Sheykhhasan ◽

Amelia Seifalian ◽

Alexander Seifalian

Keyword(s):

Growth Factors ◽

Growth Factor ◽

Regenerative Medicine ◽

Biological Therapy ◽

Signalling Molecules ◽

Bioactive Agents ◽

Essential Growth ◽

Essential Growth Factor ◽

Potential Use ◽

Active Substances

The potential use of growth factors in stem cell-based therapies for the repair and regeneration of tissues and organs offers a paradigm shift in regenerative medicine. Growth factors are critical signalling molecules that play an important role in tissue development and remodelling. Plasma Rich in Growth Factor (PRGF) is a biotechnological strategy for the harvesting of the active substances of platelets, including growth factors, from the patient’s blood. Because of their tremendous essential growth factor and bioactive agents, as well as their paracrine mechanisms, PRGF has been used as an efficacious option and adjuvant biological therapy in the repair and replacement of damaged organs. This article provides an overview of PRGF extraction and its properties and critically reviewed its clinical benefit and clinical trials in the treatment and regeneration of human organs. Regenerative medicine is a multi-billion-dollar industry with huge interest to clinicians, academics and industries, being considered as an emerging technology.

Download Full-text