Light-powered CO2 fixation in a chloroplast mimic with natural and synthetic parts

Nature integrates complex biosynthetic and energy-converting tasks within compartments such as chloroplasts and mitochondria. Chloroplasts convert light into chemical energy, driving carbon dioxide fixation. We used microfluidics to develop a chloroplast mimic by encapsulating and operating photosynthetic membranes in cell-sized droplets. These droplets can be energized by light to power enzymes or enzyme cascades and analyzed for their catalytic properties in multiplex and real time. We demonstrate how these microdroplets can be programmed and controlled by adjusting internal compositions and by using light as an external trigger. We showcase the capability of our platform by integrating the crotonyl–coenzyme A (CoA)/ethylmalonyl-CoA/hydroxybutyryl-CoA (CETCH) cycle, a synthetic network for carbon dioxide conversion, to create an artificial photosynthetic system that interfaces the natural and the synthetic biological worlds.

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Effect of MoO3 on the Catalytic Properties of NiO/Al2O3 in the Carbon Dioxide Conversion of Methane

Theoretical and Experimental Chemistry ◽

10.1007/s11237-019-09605-6 ◽

2019 ◽

Vol 55 (2) ◽

pp. 137-142 ◽

Cited By ~ 2

Author(s):

K. Dossumov ◽

G. E. Ergazieva ◽

L. K. Myltykbaeva ◽

M. M. Telbaeva ◽

A. T. Batyrbaev

Keyword(s):

Carbon Dioxide ◽

Catalytic Properties ◽

Carbon Dioxide Conversion ◽

Conversion Of Methane

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Visible light-induced reduction of carbon dioxide sensitized by a porphyrin–rhenium dyad metal complex on p-type semiconducting NiO as the reduction terminal end of an artificial photosynthetic system

Journal of Catalysis ◽

10.1016/j.jcat.2013.03.025 ◽

2014 ◽

Vol 310 ◽

pp. 57-66 ◽

Cited By ~ 83

Author(s):

Youki Kou ◽

Shogo Nakatani ◽

Gen Sunagawa ◽

Yasuyuki Tachikawa ◽

Dai Masui ◽

...

Keyword(s):

Carbon Dioxide ◽

Metal Complex ◽

Visible Light ◽

Photosynthetic System ◽

Artificial Photosynthetic ◽

P Type

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Comparison of carbon sequestration efficacy between artificial photosynthetic carbon dioxide conversion and timberland reforestation

MRS Energy & Sustainability ◽

10.1557/mre.2020.32 ◽

2020 ◽

Vol 7 ◽

Author(s):

Santiago Gonzalez Hernandez ◽

Stafford W. Sheehan

Keyword(s):

Carbon Dioxide ◽

Carbon Sequestration ◽

Carbon Dioxide Conversion ◽

Photosynthetic Carbon ◽

Artificial Photosynthetic

Abstract

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A review on recent developments in solar photoreactors for carbon dioxide conversion to fuels

Journal of CO2 Utilization ◽

10.1016/j.jcou.2021.101515 ◽

2021 ◽

Vol 47 ◽

pp. 101515 ◽

Cited By ~ 1

Author(s):

Angel Francis ◽

Shanmuga Priya S. ◽

Harish Kumar S ◽

Sudhakar K ◽

Muhammad Tahir

Keyword(s):

Carbon Dioxide ◽

Carbon Dioxide Conversion ◽

Recent Developments

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Single layered hollow NiO–NiS catalyst with large specific surface area and highly efficient visible-light-driven carbon dioxide conversion

Chemosphere ◽

10.1016/j.chemosphere.2021.130759 ◽

2021 ◽

pp. 130759

Author(s):

Byung Hyun Park ◽

Minkyu Kim ◽

No-Kuk Park ◽

Ho-Jung Ryu ◽

Jeom‐In Baek ◽

...

Keyword(s):

Carbon Dioxide ◽

Visible Light ◽

Specific Surface ◽

Surface Area ◽

Specific Surface Area ◽

Large Specific Surface Area ◽

Carbon Dioxide Conversion ◽

Highly Efficient ◽

Visible Light Driven

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Estimation of the real-time infiltration rate using a low carbon dioxide concentration

Journal of Building Engineering ◽

10.1016/j.jobe.2021.102835 ◽

2021 ◽

pp. 102835

Author(s):

Sowoo Park ◽

Paul Choi ◽

Doosam Song ◽

Junemo Koo

Keyword(s):

Carbon Dioxide ◽

Real Time ◽

Carbon Dioxide Concentration ◽

Infiltration Rate ◽

Low Carbon ◽

The Real

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Assessment of Voltage Influence in Carbon Dioxide Fixation Process by a Photo-Bioelectrochemical System under Photoheterotrophy

Microorganisms ◽

10.3390/microorganisms9030474 ◽

2021 ◽

Vol 9 (3) ◽

pp. 474

Author(s):

Sara Díaz-Rullo Edreira ◽

Silvia Barba ◽

Ioanna A. Vasiliadou ◽

Raúl Molina ◽

Juan Antonio Melero ◽

...

Keyword(s):

Carbon Dioxide ◽

Carbon Source ◽

Future Development ◽

Metabolic Pathways ◽

Co2 Fixation ◽

Oxygen Demand ◽

Carbon Uptake ◽

Bioelectrochemical System ◽

Electron Sink ◽

Soluble Carbon

Bioelectrochemical systems are a promising technology capable of reducing CO2 emissions, a renewable carbon source, using electroactive microorganisms for this purpose. Purple Phototrophic Bacteria (PPB) use their versatile metabolism to uptake external electrons from an electrode to fix CO2. In this work, the effect of the voltage (from −0.2 to −0.8 V vs. Ag/AgCl) on the metabolic CO2 fixation of a mixed culture of PPB under photoheterotrophic conditions during the oxidation of a biodegradable carbon source is demonstrated. The minimum voltage to fix CO2 was between −0.2 and −0.4 V. The Calvin–Benson–Bassham (CBB) cycle is the main electron sink at these voltages. However, lower voltages caused the decrease in the current intensity, reaching a minimum at −0.8 V (−4.75 mA). There was also a significant relationship between the soluble carbon uptake in terms of chemical oxygen demand and the electron consumption for the experiments performed at −0.6 and −0.8 V. These results indicate that the CBB cycle is not the only electron sink and some photoheterotrophic metabolic pathways are also being affected under electrochemical conditions. This behavior has not been tested before in photoheterotrophic conditions and paves the way for the future development of photobioelectrochemical systems under heterotrophic conditions.

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