scholarly journals Breeding a fungal gene into wheat

Science ◽  
2020 ◽  
Vol 368 (6493) ◽  
pp. 822-823
Author(s):  
Brande B. H. Wulff ◽  
Jonathan D. G. Jones
Keyword(s):  
Fungal Gene

TaF: a web platform for taxonomic profile-based fungal gene prediction

2018 ◽  
Vol 41 (3) ◽  
pp. 337-342
Author(s):  
Sin-Gi Park ◽  
DongSung Ryu ◽  
Hyunsung Lee ◽  
Hojin Ryu ◽  
Yong Ju Ahn ◽  
...  
Keyword(s):  
Gene Prediction ◽  
Taxonomic Profile ◽  
Web Platform ◽  
Fungal Gene

scholarly journals The fungal gene cluster for biosynthesis of the antibacterial agent viriditoxin

2019 ◽  
Author(s):  
Andrew S. Urquhart ◽  
Jinyu Hu ◽  
Yit-Heng Chooi ◽  
Alexander Idnurm
Keyword(s):  
Secondary Metabolites ◽  
Gene Cluster ◽  
Gene Disruption ◽  
Biosynthetic Pathway ◽  
Fluorescent Protein ◽  
Model Species ◽  
Paecilomyces Variotii ◽  
Bioinformatic Approaches ◽  
Green Fluorescent ◽  
Fungal Gene

AbstractBackgroundViriditoxin is one of the ‘classical’ secondary metabolites produced by fungi and that has antibacterial and other activities; however, the mechanism of its biosynthesis has remained unknown.ResultsHere, a gene cluster responsible for its synthesis was identified, using bioinformatic approaches from two species that produce viriditoxin and then through gene disruption and metabolite profiling. All eight genes in the cluster inPaecilomyces variotiiwere mutated, revealing their roles in the synthesis of this molecule and establishing its biosynthetic pathway which includes an interesting Baeyer-Villiger monooxygenase catalyzed reaction. Additionally, a candidate catalytically-inactive hydrolase was identified as being required for the stereoselective biosynthesis of (M)-viriditoxin. The localization of two proteins were assessed by fusing these proteins to green fluorescent protein, revealing that at least two intracellular structures are involved in the compartmentalization of the synthesis steps of this metabolite.ConclusionsThe full pathway for synthesis of viriditoxin was established by a combination of genomics, bioinformatics, gene disruption and chemical analysis processes. Hence, this work reveals the basis for the synthesis of an understudied class of fungal secondary metabolites and provides a new model species for understanding the synthesis of biaryl compounds with a chiral axis.

scholarly journals In Planta Stage-Specific Fungal Gene Profiling Elucidates the Molecular Strategies of Fusarium graminearum Growing inside Wheat Coleoptiles

2012 ◽  
Vol 24 (12) ◽  
pp. 5159-5176 ◽  
Author(s):  
Xiao-Wei Zhang ◽  
Lei-Jie Jia ◽  
Yan Zhang ◽  
Gang Jiang ◽  
Xuan Li ◽  
...  
Keyword(s):  
Fusarium Graminearum ◽  
Gene Profiling ◽  
In Planta ◽  
Fungal Gene

scholarly journals An Approach to the Production of Soluble Protein from a Fungal Gene Encoding an Aggregation-Prone Xylanase in Escherichia coli

PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. e18489 ◽  
Author(s):  
Yilin Le ◽  
Jingjing Peng ◽  
Huawei Wu ◽  
Jianzhong Sun ◽  
Weilan Shao
Keyword(s):  
Escherichia Coli ◽  
Soluble Protein ◽  
Gene Encoding ◽  
Fungal Gene

scholarly journals Differential accumulation of poly(A)+ RNA between virulent and double-stranded RNA-induced hypovirulent strains of Cryphonectria (Endothia) parasitica.

1987 ◽  
Vol 7 (10) ◽  
pp. 3688-3693 ◽  
Author(s):  
W A Powell ◽  
N K Van Alfen
Keyword(s):  
Gene Expression ◽  
Specific Effect ◽  
Rna Sequences ◽  
Double Stranded Rna ◽  
Total Rna ◽  
Rna Sequence ◽  
Differential Hybridization ◽  
Endothia Parasitica ◽  
Fungal Gene Expression ◽  
Fungal Gene

The double-stranded RNA responsible for transmissible hypovirulence in Cryphonectria (Endothia) parasitica was found to affect the accumulation of specific poly(A)+ RNA. Using differential hybridization techniques, two genes were isolated, Vir1 and Vir2, which were specifically expressed as poly(A)+ RNAs in the virulent cells. The highly expressed RNA sequences from these genes were not found in total RNA isolated from either American or European hypovirulent strains, although the genes were present in their genomes. Other virulence- and hypovirulence-specific RNA sequences were also detected. One isolated hypovirulence-specific RNA sequence was expressed in both virulent and hypovirulent cells, but in a two- to fourfold-higher concentration in the hypovirulent cells. The results show that hypovirulence is associated with concurrent changes in a few highly expressed poly(A)+ RNAs, which suggests a specific effect of the double-stranded RNA on fungal gene expression.

A fungal gene for antibiotic resistance on a dispensable ("B") chromosome

Science ◽  
1991 ◽  
Vol 254 (5039) ◽  
pp. 1773-1776 ◽  
Author(s):  
V. Miao ◽  
S. Covert ◽  
H. VanEtten
Keyword(s):  
Antibiotic Resistance ◽  
B Chromosome ◽  
Fungal Gene

Fungal Gene-Encoded Peptidase Inhibitors

2013 ◽  
Vol 20 (25) ◽  
pp. 3041-3048
Author(s):  
V. Bauerova ◽  
I. Pichova ◽  
O. Hrukova-Heidingsfeldova
Keyword(s):  
Peptidase Inhibitors ◽  
Fungal Gene
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