scholarly journals Antimicrobial Susceptibility of Mycoplasma pneumoniae Isolates and Molecular Analysis of Macrolide-Resistant Strains from Shanghai, China

2009 ◽  
Vol 53 (5) ◽  
pp. 2160-2162 ◽  
Author(s):  
Yang Liu ◽  
Xinyu Ye ◽  
Hong Zhang ◽  
Xiaogang Xu ◽  
Wanhua Li ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Fragment Length Polymorphism ◽  
Clinical Isolates ◽  
Rrna Genes ◽  
23S Rrna ◽  
Type I ◽  
Resistant Strains ◽  
23S Rrna Genes ◽  
Transition Mutation ◽  
Gene Restriction

ABSTRACT Fifty-three Mycoplasma pneumoniae strains were isolated from pediatric patients in Shanghai, China, from October 2005 to February 2008. Of 53 clinical isolates, 44 (83%) were resistant to erythromycin (MICs of >128 μg/ml for all 44 strains), azithromycin, and clarithromycin. All macrolide-resistant M. pneumoniae strains harbored an A-to-G transition mutation at position 2063 in 23S rRNA genes. Forty-five (85%) clinical isolates were classified into the P1 gene restriction fragment length polymorphism type I, and six (11%) were type II.

scholarly journals Acquired macrolide resistance in the human intestinal strain Lactobacillus rhamnosus E41 associated with a transition mutation in 23S rRNA genes

2007 ◽  
Vol 30 (4) ◽  
pp. 341-344 ◽  
Author(s):  
Ana-Belén Flórez ◽  
Víctor Ladero ◽  
Pablo Álvarez-Martín ◽  
Mohammed-Salim Ammor ◽  
Miguel-Ángel Álvarez ◽  
...  
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Macrolide Resistance ◽  
Rrna Genes ◽  
23S Rrna ◽  
23S Rrna Genes ◽  
Transition Mutation

scholarly journals Gene Dosage and Linezolid Resistance in Enterococcus faecium and Enterococcus faecalis

2002 ◽  
Vol 46 (10) ◽  
pp. 3334-3336 ◽  
Author(s):  
S. H. Marshall ◽  
C. J. Donskey ◽  
R. Hutton-Thomas ◽  
R. A. Salata ◽  
L. B. Rice
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Gene Dosage ◽  
Clinical Isolates ◽  
Rrna Genes ◽  
23S Rrna ◽  
Linezolid Resistance ◽  
Clear Association ◽  
23S Rrna Genes ◽  
Domain V

ABSTRACT Resistance to linezolid has been associated with a G2576U mutation in domain V of the 23S rRNA. We analyzed nine clinical isolates of linezolid-resistant enterococci and showed a clear association between the number of 23S rRNA genes containing this mutation and the level of linezolid resistance expressed.

scholarly journals Rapid Detection and Estimation by Pyrosequencing of 23S rRNA Genes with a Single Nucleotide Polymorphism Conferring Linezolid Resistance in Enterococci

2003 ◽  
Vol 47 (11) ◽  
pp. 3620-3622 ◽  
Author(s):  
Alistair Sinclair ◽  
Catherine Arnold ◽  
Neil Woodford
Keyword(s):  
Fragment Length Polymorphism ◽  
Rrna Genes ◽  
23S Rrna ◽  
Rrna Gene ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Linezolid Resistance ◽  
Gene Copies ◽  
23S Rrna Gene ◽  
23S Rrna Genes

ABSTRACT Pyrosequencing was used to detect rapidly and estimate the number of 23S rRNA genes with a G2576T mutation in 43 linezolid-resistant and -susceptible clinical isolates of enterococci. The method showed 100% concordance with PCR-restriction fragment length polymorphism for detecting isolates homozygous for either G2576 or T2576 or heterozygous for this mutation. A good correlation was found between linezolid MICs and the number of 23S rRNA gene copies carrying the mutation.

Leuconostoc Spoilage of Vacuum-Packaged Vegetable Sausages

2008 ◽  
Vol 71 (11) ◽  
pp. 2312-2315 ◽  
Author(s):  
ELINA J. VIHAVAINEN ◽  
ANNA E. MURROS ◽  
K. JOHANNA BJÖRKROTH
Keyword(s):  
Leuconostoc Mesenteroides ◽  
Fragment Length Polymorphism ◽  
Rrna Genes ◽  
23S Rrna ◽  
Operational Taxonomic Units ◽  
Fresh Vegetable ◽  
23S Rrna Genes ◽  
Leuconostoc Gelidum ◽  
Restriction Fragment Length

The present study was conducted to assess the role of lactic acid bacteria (LAB) in spoilage of a vacuum-packaged vegetable sausage product. This spoilage problem was characterized by formation of gas and slime, and was limiting the shelf life of the product. To investigate the LAB populations, LAB were enumerated in vegetable sausages graded as either spoiled or acceptable. From these vegetable sausages, 110 prevailing LAB isolates were recovered and identified using an LAB ribotyping database, which uses HindIII restriction fragment length polymorphism patterns of the 16S and 23S rRNA genes as operational taxonomic units. Finally, to determine the effects of the prevailing LAB on the sensory properties of the product, fresh vegetable sausages were inoculated with six LAB strains. The results revealed that Leuconostoc gelidum, Leuconostoc gasicomitatum, and Leuconostoc mesenteroides were the predominant LAB in the commercial vegetable sausages. The inoculation of these LAB onto vegetable sausages resulted in the formation of gas, slime, and a sour off-odor. Based on these findings, L. gelidum, L. gasicomitatum, and L. mesenteroides were responsible for spoilage of the vegetable sausage product.

scholarly journals Macrolide-Resistant Mycoplasma pneumoniae in Adults in Zhejiang, China

2014 ◽  
Vol 59 (2) ◽  
pp. 1048-1051 ◽  
Author(s):  
Zibo Zhou ◽  
Xiangzhi Li ◽  
Xiaojian Chen ◽  
Fangjun Luo ◽  
Changwang Pan ◽  
...  
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Rflp Analysis ◽  
Zhejiang Province ◽  
23S Rrna ◽  
Rrna Gene ◽  
Type I ◽  
Content Type ◽  
23S Rrna Gene ◽  
Resistant Strains ◽  
Domain V

ABSTRACTMycoplasma pneumoniaeis a major pathogen causing community-acquired pneumoniae (CAP), which is generally treated with macrolides. In recent years, however, although macrolide-resistantM. pneumoniaehas been reported frequently, particularly in China, very little is known about the prevalence of macrolide-resistantM. pneumoniaeinfection in adults. In this study, we survey the macrolide-resistantM. pneumoniaein adults in Zhejiang province and characterize the mechanisms of resistance to macrolide. Six hundred fifty throat swab samples were collected from adult patients with CAP from January 2012 to August 2014. These samples were assayed by nested PCR and then cultivated forM. pneumoniae. All isolates were sequenced to determine the mutation in domain V of the 23S rRNA gene. The activities of 10 antibiotics against macrolide-resistantM. pneumoniaeisolates were also investigatedin vitro. Moreover, restriction fragment length polymorphism (RFLP) analysis of the amplified P1 gene was used to type 50 resistant strains. One hundred percent (71/71) ofM. pneumoniaestrains isolated from adults with CAP were resistant to erythromycin (MIC = 128 to >256 μg/ml), clarithromycin (MIC = 128 to >256 μg/ml), and azithromycin (MIC = 32 to >64 μg/ml). Furthermore, all macrolide-resistantM. pneumoniaestrains identified had an A2063G mutation in domain V of the 23S rRNA gene. Forty-six resistant strains (92.0%) were classified into type I strain on the basis of P1 gene PCR-RFLP analysis. According to these findings, it is suggested that macrolide-resistantM. pneumoniaeinfection is very prevalence among adults in Zhejiang province. Thus, there is necessary to perform the epidemiological monitoring of macrolide-resistantM. pneumoniaein the future.

Demonstration of the absence of intervening sequences within 23S rRNA genes fromCampylobacter lari

2009 ◽  
Vol 49 (4) ◽  
pp. 386-394 ◽  
Author(s):  
Akihiro Tazumi ◽  
Yuki Kakinuma ◽  
John E. Moore ◽  
Cherie B. Millar ◽  
Ikue Taneike ◽  
...  
Keyword(s):  
Rrna Genes ◽  
23S Rrna ◽  
Intervening Sequences ◽  
23S Rrna Genes

scholarly journals tRNA genes are found between the 16S and 23S rRNA genes in Bacillus subtilis

1982 ◽  
Vol 10 (5) ◽  
pp. 1607-1624 ◽  
Author(s):  
Kate Loughney ◽  
Elsebet Lund ◽  
James E. Dahlberg
Keyword(s):  
Bacillus Subtilis ◽  
Rrna Genes ◽  
23S Rrna ◽  
Trna Genes ◽  
23S Rrna Genes

scholarly journals Spontaneous Erythromycin Resistance Mutation in a 23S rRNA Gene, rrlA, of the Extreme Thermophile Thermus thermophilus IB-21

2001 ◽  
Vol 183 (14) ◽  
pp. 4382-4385 ◽  
Author(s):  
Steven T. Gregory ◽  
Jamie H. D. Cate ◽  
Albert E. Dahlberg
Keyword(s):  
Genetic Manipulation ◽  
Thermus Thermophilus ◽  
Resistance Mutation ◽  
Rrna Genes ◽  
23S Rrna ◽  
Rrna Gene ◽  
Erythromycin Resistance ◽  
23S Rrna Gene ◽  
Resistant Mutants ◽  
23S Rrna Genes

ABSTRACT Spontaneous, erythromycin-resistant mutants of Thermus thermophilus IB-21 were isolated and found to carry the mutation A2058G in one of two 23S rRNA operons. The heterozygosity of these mutants indicates that A2058G confers a dominant or codominant phenotype in this organism. This mutation provides a valuable tool for the genetic manipulation of the 23S rRNA genes ofThermus.

scholarly journals Heterogeneous Macrolide Resistance and Gene Conversion in the Pneumococcus

2006 ◽  
Vol 50 (1) ◽  
pp. 359-361 ◽  
Author(s):  
Nicole Wolter ◽  
Anthony M. Smith ◽  
David J. Farrell ◽  
Keith P. Klugman
Keyword(s):  
Streptococcus Pneumoniae ◽  
Gene Conversion ◽  
Clinical Isolate ◽  
Resistant Strain ◽  
Macrolide Resistance ◽  
Fitness Cost ◽  
Rrna Genes ◽  
23S Rrna ◽  
Resistant Phenotype ◽  
23S Rrna Genes

ABSTRACT A macrolide-resistant clinical isolate of Streptococcus pneumoniae with 23S rRNA mutations showed a heterogeneous phenotype and genotype. The mutant 23S rRNA genes from this isolate transformed susceptible strain R6 to resistance. Culture of resistant strain R6 in the absence of antibiotic pressure showed gene conversion to occur between the four 23S rRNA alleles, resulting in reversion to susceptibility with the resistant phenotype showing a fitness cost. These data explain the disappearance on subculture of heterogeneous macrolide resistance in the pneumococcus.

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