scholarly journals Treatment of Pseudomonas aeruginosa-infected orthopedic prostheses with ceftazidime-ciprofloxacin antibiotic combination

1995 ◽  
Vol 39 (11) ◽  
pp. 2423-2425 ◽  
Author(s):  
P. Brouqui ◽  
M. C. Rousseau ◽  
A. Stein ◽  
M. Drancourt ◽  
D. Raoult
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Combination

scholarly journals An In Vitro Coumarin-Antibiotic Combination Treatment of Pseudomonas aeruginosa Biofilms

2021 ◽  
Vol 16 (1) ◽  
pp. 1934578X2098774
Author(s):  
Jinpeng Zou ◽  
Yang Liu ◽  
Ruiwei Guo ◽  
Yu Tang ◽  
Zhengrong Shi ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drug Resistance ◽  
Combination Treatment ◽  
Crude Extract ◽  
Biofilm Formation ◽  
Bacterial Resistance ◽  
Antibacterial Properties ◽  
Biofilm Growth ◽  
Antibiotic Combination

The drug resistance of Pseudomonas aeruginosa is a worldwide problem due to its great threat to human health. A crude extract of Angelica dahurica has been proved to have antibacterial properties, which suggested that it may be able to inhibit the biofilm formation of P. aeruginosa; initial exploration had shown that the crude extract could inhibit the growth of P. aeruginosa effectively. After the adaptive dose of coumarin was confirmed to be a potential treatment for the bacteria’s drug resistance, “coumarin-antibiotic combination treatments” (3 coumarins—simple coumarin, imperatorin, and isoimperatorin—combined with 2 antibiotics—ampicillin and ceftazidime) were examined to determine their capability to inhibit P. aeruginosa. The final results showed that (1) coumarin with either ampicillin or ceftazidime significantly inhibited the biofilm formation of P. aeruginosa; (2) coumarin could directly destroy mature biofilms; and (3) the combination treatment can synergistically enhance the inhibition of biofilm formation, which could significantly reduce the usage of antibiotics and bacterial resistance. To sum up, a coumarin-antibiotic combination treatment may be a potential way to inhibit the biofilm growth of P. aeruginosa and provides a reference for antibiotic resistance treatment.

scholarly journals Efficacy of Antibiotic Combinations against Multidrug-Resistant Pseudomonas aeruginosa in Automated Time-Lapse Microscopy and Static Time-Kill Experiments

2020 ◽  
Vol 64 (6) ◽  
Author(s):  
Anna Olsson ◽  
Pikkei Wistrand-Yuen ◽  
Elisabet I. Nielsen ◽  
Lena E. Friberg ◽  
Linus Sandegren ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Time Lapse ◽  
Polymyxin B ◽  
Multidrug Resistant ◽  
Positive Interactions ◽  
Synergistic Activity ◽  
Content Type ◽  
Time Lapse Microscopy ◽  
Time Kill ◽  
Antibiotic Combination

ABSTRACT Antibiotic combination therapy is used for severe infections caused by multidrug-resistant (MDR) Gram-negative bacteria, yet data regarding which combinations are most effective are lacking. This study aimed to evaluate the in vitro efficacy of polymyxin B in combination with 13 other antibiotics against four clinical strains of MDR Pseudomonas aeruginosa. We evaluated the interactions of polymyxin B in combination with amikacin, aztreonam, cefepime, chloramphenicol, ciprofloxacin, fosfomycin, linezolid, meropenem, minocycline, rifampin, temocillin, thiamphenicol, or trimethoprim by automated time-lapse microscopy using predefined cutoff values indicating inhibition of growth (≤106 CFU/ml) at 24 h. Promising combinations were subsequently evaluated in static time-kill experiments. All strains were intermediate or resistant to polymyxin B, antipseudomonal β-lactams, ciprofloxacin, and amikacin. Genes encoding β-lactamases (e.g., blaPAO and blaOXA-50) and mutations associated with permeability and efflux were detected in all strains. In the time-lapse microscopy experiments, positive interactions were found with 39 of 52 antibiotic combination/bacterial strain setups. Enhanced activity was found against all four strains with polymyxin B used in combination with aztreonam, cefepime, fosfomycin, minocycline, thiamphenicol, and trimethoprim. Time-kill experiments showed additive or synergistic activity with 27 of the 39 tested polymyxin B combinations, most frequently with aztreonam, cefepime, and meropenem. Positive interactions were frequently found with the tested combinations, against strains that harbored several resistance mechanisms to the single drugs, and with antibiotics that are normally not active against P. aeruginosa. Further study is needed to explore the clinical utility of these combinations.

scholarly journals Effectiveness of Commonly Used Antibiotics in Combination with Honey Against Bacterial Infection

2020 ◽  
Vol 7 ◽  
pp. 51-61
Author(s):  
Niraj Shrestha ◽  
Shova Shrestha ◽  
Bharat Mani Pokharel
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacterial Infection ◽  
Drug Resistant ◽  
Inhibitory Effects ◽  
Acinetobacter Species ◽  
The Difference ◽  
Antibiotic Combination

Objectives: The study was carried out to compare the inhibitory effects between commonly used antibiotics and bee honey samples, so as to correlate the inhibitory effects between bee honey alone and in combination with antibiotics. Methods: This study was carried out between December 2012 to September 2013. A total of one hundred and twenty-two clinical microbiological specimens and five different floral sourced honey samples were collected between December 2012 to September 2013. Twenty-three multi-drug resistant organisms were selected. Then, AST for commonly used antibiotics, honey alone and combination of honey-antibiotics discs was done. The difference in ZOI of antibiotic contrasting with the antibiotics containing honey were statistically analysed to define the synergism. Results: The inhibition due to honey is variable among bacteria types (F=39.17, p<0.05). From means plot, Staphylococcus and Acinetobacter were recognized as highly susceptible bacteria for honey (Χ = 21.1 ± 6.2 mm and Χ = 18.3 ± 3.3 mm respectively) but Acinetobacter species could not show synergism to honey-antibiotic combination. The tested organisms from Enterobacteriaceae family showed effective susceptibility to Chloramphenicol-honey mixture. Imipenem-honey combination and Gentamicin-honey combination showed significant effects against Pseudomonas aeruginosa. Conclusion: Thus, honey can be used in various bacteria-directed infections and found to be effective in various infections. Incorporation of honey in antibiotics like Chloramphenicol, Imipenem, and Gentamicin work better in healing various infection.  

PcrV antibody–antibiotic combination improves survival in Pseudomonas aeruginosa-infected mice

2011 ◽  
Vol 31 (8) ◽  
pp. 1837-1845 ◽  
Author(s):  
Y. Song ◽  
M. Baer ◽  
R. Srinivasan ◽  
J. Lima ◽  
G. Yarranton ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Combination

scholarly journals Antibiotic combination therapy can select for broad-spectrum multidrug resistance in Pseudomonas aeruginosa

2016 ◽  
Vol 47 (1) ◽  
pp. 48-55 ◽  
Author(s):  
Martin Vestergaard ◽  
Wilhelm Paulander ◽  
Rasmus L. Marvig ◽  
Julie Clasen ◽  
Nicholas Jochumsen ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Multidrug Resistance ◽  
Combination Therapy ◽  
Broad Spectrum ◽  
Antibiotic Combination

Monoclonal antibody directed to the PilQ -PilA DSL region in Pseudomonas aeruginosa improves survival of infected mice with antibiotic combination

2021 ◽  
pp. 105060
Author(s):  
Abed Zahedi bialvaei ◽  
Shabnam Razavi ◽  
Farzaneh Notash Haghighat ◽  
Azam Hemmati ◽  
Maziar Mohammad Akhavan ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Combination

scholarly journals A Case of Sepsis Caused by Multidrug-resistant Neutropenic-phase Pseudomonas aeruginosa Treated Successfully with Antibiotic Combination Therapy

2008 ◽  
Vol 82 (5) ◽  
pp. 466-470
Author(s):  
Hideki ARAOKA ◽  
Masaru BABA ◽  
Keita TATSUSHIMA ◽  
Shinsuke TAKAGI ◽  
Naofumi MATSUNO ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Combination Therapy ◽  
Multidrug Resistant ◽  
Antibiotic Combination

scholarly journals Synergistic Killing and Re-Sensitization of Pseudomonas aeruginosa to Antibiotics by Phage-Antibiotic Combination Treatment

2021 ◽  
Vol 14 (3) ◽  
pp. 184
Author(s):  
Emily Engeman ◽  
Helen R. Freyberger ◽  
Brendan W. Corey ◽  
Amanda M. Ward ◽  
Yunxiu He ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Combination Treatment ◽  
Multidrug Resistant ◽  
Wound Model ◽  
Colony Forming Units ◽  
Genes Encoding ◽  
Worm Model ◽  
Antibiotic Combination

Multidrug-resistant (MDR) Pseudomonas aeruginosa infections pose a serious health threat. Bacteriophage–antibiotic combination therapy is a promising candidate for combating these infections. A 5-phage P. aeruginosa cocktail, PAM2H, was tested in combination with antibiotics (ceftazidime, ciprofloxacin, gentamicin, meropenem) to determine if PAM2H enhances antibiotic activity. Combination treatment in vitro resulted in a significant increase in susceptibility of MDR strains to antibiotics. Treatment with ceftazidime (CAZ), meropenem, gentamicin, or ciprofloxacin in the presence of the phage increased the number of P. aeruginosa strains susceptible to these antibiotics by 63%, 56%, 31%, and 81%, respectively. Additionally, in a mouse dorsal wound model, seven of eight mice treated with a combination of CAZ and PAM2H for three days had no detectable bacteria remaining in their wounds on day 4, while all mice treated with CAZ or PAM2H alone had ~107 colony forming units (CFU) remaining in their wounds. P. aeruginosa recovered from mouse wounds post-treatment showed decreased virulence in a wax worm model, and DNA sequencing indicated that the combination treatment prevented mutations in genes encoding known phage receptors. Treatment with PAM2H in combination with antibiotics resulted in the re-sensitization of P. aeruginosa to antibiotics in vitro and a synergistic reduction in bacterial burden in vivo.

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