Detection of gyrA Mutations among 335Pseudomonas aeruginosa Strains Isolated in Japan and Their Susceptibilities to Fluoroquinolones
1999 ◽
Vol 43
(2)
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pp. 406-409
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Keyword(s):
ABSTRACT gyrA point mutations in 335 clinical Pseudomonas aeruginosa isolates were examined mainly by nonisotopic single-strand conformation polymorphism analysis and direct sequencing. Seven types of missense gyrA mutations were observed in 70 of 335 strains (20.9%), and ciprofloxacin MICs were ≥3.13 μg/ml for 63 of 70 strains (90.0%). These included two double point mutations and three novel mutations (Ala-67→Ser plus Asp-87→Gly, Ala-84→Pro, and Gln-106→Leu). Thr-83→Ile mutants were predominantly observed (63 of 70 mutants) and showed high-level fluoroquinolone resistance (ciprofloxacin MIC at which 50% of isolates are inhibited, 25 μg/ml).
1996 ◽
Vol 26
(6)
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pp. 417-421
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1994 ◽
Vol 38
(3)
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pp. 428-431
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1992 ◽
Vol 111
(2)
◽
pp. 222-226
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1996 ◽
Vol 19
(1)
◽
pp. 93-94
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