scholarly journals Potent In Vitro Synergism of Fluconazole and Berberine Chloride against Clinical Isolates of Candida albicans Resistant to Fluconazole

2006 ◽  
Vol 50 (3) ◽  
pp. 1096-1099 ◽  
Author(s):  
Hua Quan ◽  
Ying-Ying Cao ◽  
Zheng Xu ◽  
Jing-Xia Zhao ◽  
Ping-Hui Gao ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Clinical Isolates ◽  
Antagonistic Action ◽  
Berberine Chloride ◽  
Agar Diffusion ◽  
Fungistatic Activity ◽  
Fluconazole Resistant ◽  
Time Kill ◽  
In Vitro Interaction

ABSTRACT In vitro interaction of fluconazole and berberine chloride was investigated against 40 fluconazole-resistant clinical isolates of Candida albicans. Synergism in fungistatic activity was found with the checkerboard microdilution assay. The findings of agar diffusion tests and time-kill curves confirmed the synergistic interaction, but no antagonistic action was observed.

scholarly journals In VitroInteraction between Fluconazole and Triclosan against Clinical Isolates of Fluconazole-Resistant Candida albicans Determined by Different Methods

2011 ◽  
Vol 55 (7) ◽  
pp. 3609-3612 ◽  
Author(s):  
Lu Yu ◽  
Guanghui Ling ◽  
Xuming Deng ◽  
Jing Jin ◽  
Qi Jin ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antagonistic Activity ◽  
Clinical Isolates ◽  
Content Type ◽  
Agar Diffusion ◽  
Fluconazole Resistant

ABSTRACTThein vitrointeraction between triclosan and fluconazole against 24 azole-resistant clinical isolates ofCandida albicanswas evaluated by the microdilution checkerboard technique. The synergisms were verified by time-killing curves and agar diffusion tests in selected strains. Antagonistic activity was not detected.

scholarly journals Antifungal activity of thymol against clinical isolates of fluconazole-sensitive and -resistant Candida albicans

2009 ◽  
Vol 58 (8) ◽  
pp. 1074-1079 ◽  
Author(s):  
Na Guo ◽  
Jingbo Liu ◽  
Xiuping Wu ◽  
Xingming Bi ◽  
Rizeng Meng ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Concentration Index ◽  
Inhibitory Concentration ◽  
Clinical Isolates ◽  
Standard Strain ◽  
Antagonistic Action ◽  
Microdilution Method ◽  
Interaction Intensity

Thymol (THY) was found to have in vitro antifungal activity against 24 fluconazole (FLC)-resistant and 12 FLC-susceptible clinical isolates of Candida albicans, standard strain ATCC 10231 and one experimentally induced FLC-resistant C. albicans S-1. In addition, synergism was observed for clinical isolates of C. albicans with combinations of THY–FLC and THY–amphotericin B (AMB) evaluated by the chequerboard microdilution method. The interaction intensity was determined by spectrophotometry for the chequerboard assay, and the nature of the interactions was assessed using two non-parametric approaches [fractional inhibitory concentration index (FICI) and ΔE models]. The interaction between THY–FLC or THY–AMB in FLC-resistant and -susceptible strains of C. albicans showed a high percentage of synergism by the FICI method and the ΔE method. The ΔE model gave results consistent with FICI, and no antagonistic action was observed in the strains tested.

scholarly journals In Vitro Interaction of Posaconazole and Caspofungin against Clinical Isolates of Candida glabrata

2005 ◽  
Vol 49 (8) ◽  
pp. 3544-3545 ◽  
Author(s):  
E. R. Oliveira ◽  
A. W. Fothergill ◽  
W. R. Kirkpatrick ◽  
B. J. Coco ◽  
T. F. Patterson ◽  
...  
Keyword(s):  
Candida Glabrata ◽  
Concentration Index ◽  
Inhibitory Concentration ◽  
Clinical Isolates ◽  
Fractional Inhibitory Concentration ◽  
Fractional Inhibitory Concentration Index ◽  
Fluconazole Resistant ◽  
In Vitro Interaction

ABSTRACT Combinations of caspofungin and posaconazole were evaluated by fractional inhibitory concentration index against 119 Candida glabrata isolates. Synergy was seen in 18% of all isolates and in 4% of fluconazole-resistant isolates at 48 h without evidence of antagonism. This antifungal combination may have utility against this organism.

scholarly journals In-vitro interaction of terbinafine with amphotericin B, fluconazole and itraconazole against clinical isolates of Candida albicans

1998 ◽  
Vol 41 (1) ◽  
pp. 59-65 ◽  
Author(s):  
F. Barchiesi ◽  
L. F. Di Francesco ◽  
P. Compagnucci ◽  
D. Arzeni ◽  
A. Giacometti ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Amphotericin B ◽  
Clinical Isolates ◽  
In Vitro Interaction

scholarly journals In Vitro Interaction and Killing-Kinetics of Amphotericin B Combined with Anidulafungin or Caspofungin against Candida auris

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1333
Author(s):  
Unai Caballero ◽  
Elena Eraso ◽  
Guillermo Quindós ◽  
Nerea Jauregizar
Keyword(s):  
Amphotericin B ◽  
Antifungal Drugs ◽  
Candida Auris ◽  
Dose Requirement ◽  
Fungistatic Activity ◽  
Invasive Infections ◽  
Kill Curve ◽  
Time Kill ◽  
In Vitro Interaction

Treatment of invasive infections caused by Candida auris is challenging due to the limited therapeutic options. The combination of antifungal drugs may be an interesting and feasible approach to be investigated. The aim of this study was to examine the in vitro activity of amphotericin B in combination with anidulafungin or caspofungin against C. auris. In vitro static time–kill curve experiments were conducted for 48 h with different combinations of amphotericin B with anidulafungin or caspofungin against six blood isolates of C. auris. The antifungal activity of 0.5 mg/L of amphotericin B was limited against the six isolates of C. auris. Similarly, echinocandins alone had a negligible effect, even at the highest tested concentrations. By contrast, 1 mg/L of amphotericin B showed fungistatic activity. Synergy was rapidly achieved (8 h) with 0.5 mg/L of amphotericin B plus 2 mg/L of anidulafungin or caspofungin. These combinations lead to a sustained fungistatic effect, and the fungicidal endpoint was reached against some C. auris isolates. Additionally, ≥0.5 mg/L of either of the two echinocandins with 1 mg/L of amphotericin B resulted in fungicidal effect against all C. auris isolates. In conclusion, combinations of amphotericin B with anidulafungin or caspofungin provided greater killing with a lower dose requirement for amphotericin B compared to monotherapy, with synergistic and/or fungicidal outcomes.

A novel use for an old drug: resistance reversal in Candida albicans by combining dihydroartemisinin with fluconazole

2021 ◽  
Author(s):  
Hui Li ◽  
Haisheng Chen ◽  
Wenna Shi ◽  
Jing Shi ◽  
Jupeng Yuan ◽  
...  
Keyword(s):  
Drug Combination ◽  
Fungistatic Activity ◽  
Resistance Reversal ◽  
Antifungal Effects ◽  
Curve Method ◽  
Fluconazole Resistant ◽  
Kill Curve ◽  
Underlying Mechanisms ◽  
Time Kill

Aim: To investigate the effects of dihydroartemisinin combined with fluconazole against C. albicans in vitro and to explore the underlying mechanisms. Materials & methods: Checkerboard microdilution assay and time–kill curve method were employed to evaluate the static and dynamic antifungal effects against C. albicans. Reactive oxygen species (ROS) was measured by a fluorescent probe. Results: Combination of dihydroartemisinin and fluconazole exerted potent synergy against planktonic cells and biofilms of fluconazole-resistant C. albicans, with the fractional inhibitory concentration index values less than 0.07. A potent fungistatic activity of this drug combination could still be observed after 18 h. The accumulation of ROS induced by the drug combination might contribute to the synergy. Conclusion: Dihydroartemisinin reversed the resistance of C. albicans to fluconazole.

scholarly journals Azasordarins: Susceptibility of Fluconazole-Susceptible and Fluconazole-Resistant Clinical Isolates ofCandida spp. to GW 471558

2001 ◽  
Vol 45 (6) ◽  
pp. 1905-1907 ◽  
Author(s):  
Manuel Cuenca-Estrella ◽  
Emilia Mellado ◽  
Teresa M. Dı́az-Guerra ◽  
Araceli Monzón ◽  
Juan L. Rodrı́guez-Tudela
Keyword(s):  
Candida Albicans ◽  
Candida Glabrata ◽  
Candida Parapsilosis ◽  
Candida Guilliermondii ◽  
Candida Krusei ◽  
Clinical Isolates ◽  
In Vitro Activity ◽  
Fluconazole Resistant ◽  
Candida Lusitaniae

ABSTRACT The in vitro activity of the azasordarin GW 471558 was compared with those of amphotericin B, flucytosine, itraconazole, and ketoconazole against 177 clinical isolates of Candidaspp. GW 471558 showed potent activity against Candida albicans, Candida glabrata, and Candida tropicalis, even against isolates with decreased susceptibility to azoles. Candida krusei, Candida parapsilosis, Candida lusitaniae, and Candida guilliermondii are resistant to GW 471558 in vitro (MICs, >128 μg/ml).

scholarly journals Evaluation of Voriconazole Pharmacodynamics Using Time-Kill Methodology

2000 ◽  
Vol 44 (7) ◽  
pp. 1917-1920 ◽  
Author(s):  
Michael E. Klepser ◽  
Dennis Malone ◽  
Russell E. Lewis ◽  
Erika J. Ernst ◽  
Michael A. Pfaller
Keyword(s):  
Candida Albicans ◽  
Drug Concentration ◽  
Candida Glabrata ◽  
Fungal Species ◽  
Fungistatic Activity ◽  
Time Kill ◽  
The Relationship ◽  
Over Time ◽  
Time Point

ABSTRACT Voriconazole is an investigational azole antifungal agent with activity against a variety of fungal species, including fluconazole-susceptible and -resistant Candida species andCryptococcus neoformans. In this study, we employed in vitro time-kill methods to characterize the relationship between concentrations of voriconazole and its fungistatic activity againstCandida albicans, Candida glabrata,Candida tropicalis, and C. neoformans. Isolates were exposed to voriconazole concentrations ranging from 0.0625 to 16 times the MIC, and the viable colony counts were determined over time. The 50 and 90% effective concentrations (EC50 and EC90, respectively) were determined at 8, 12, and 24 h following the addition of voriconazole. At each time point, near-maximal fungistatic activity, as indicated by the EC90, was noted at a drug concentration of approximately three times the MIC. Additionally, EC50 and EC90 did not change over time, thus suggesting that the rate of activity was not improved by increasing concentrations. Voriconazole exhibits non-concentration-dependent pharmacodynamic characteristics in vitro.

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