scholarly journals Antifungal activity of thymol against clinical isolates of fluconazole-sensitive and -resistant Candida albicans

2009 ◽  
Vol 58 (8) ◽  
pp. 1074-1079 ◽  
Author(s):  
Na Guo ◽  
Jingbo Liu ◽  
Xiuping Wu ◽  
Xingming Bi ◽  
Rizeng Meng ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Concentration Index ◽  
Inhibitory Concentration ◽  
Clinical Isolates ◽  
Standard Strain ◽  
Antagonistic Action ◽  
Microdilution Method ◽  
Interaction Intensity

Thymol (THY) was found to have in vitro antifungal activity against 24 fluconazole (FLC)-resistant and 12 FLC-susceptible clinical isolates of Candida albicans, standard strain ATCC 10231 and one experimentally induced FLC-resistant C. albicans S-1. In addition, synergism was observed for clinical isolates of C. albicans with combinations of THY–FLC and THY–amphotericin B (AMB) evaluated by the chequerboard microdilution method. The interaction intensity was determined by spectrophotometry for the chequerboard assay, and the nature of the interactions was assessed using two non-parametric approaches [fractional inhibitory concentration index (FICI) and ΔE models]. The interaction between THY–FLC or THY–AMB in FLC-resistant and -susceptible strains of C. albicans showed a high percentage of synergism by the FICI method and the ΔE method. The ΔE model gave results consistent with FICI, and no antagonistic action was observed in the strains tested.

scholarly journals In Vitro Interaction of Posaconazole and Caspofungin against Clinical Isolates of Candida glabrata

2005 ◽  
Vol 49 (8) ◽  
pp. 3544-3545 ◽  
Author(s):  
E. R. Oliveira ◽  
A. W. Fothergill ◽  
W. R. Kirkpatrick ◽  
B. J. Coco ◽  
T. F. Patterson ◽  
...  
Keyword(s):  
Candida Glabrata ◽  
Concentration Index ◽  
Inhibitory Concentration ◽  
Clinical Isolates ◽  
Fractional Inhibitory Concentration ◽  
Fractional Inhibitory Concentration Index ◽  
Fluconazole Resistant ◽  
In Vitro Interaction

ABSTRACT Combinations of caspofungin and posaconazole were evaluated by fractional inhibitory concentration index against 119 Candida glabrata isolates. Synergy was seen in 18% of all isolates and in 4% of fluconazole-resistant isolates at 48 h without evidence of antagonism. This antifungal combination may have utility against this organism.

In vitro antifungal activity of luliconazole against nondermatophytic moulds

2019 ◽  
Vol 58 (5) ◽  
pp. 703-706 ◽  
Author(s):  
Jun Maeda ◽  
Hiroyasu Koga ◽  
Kou Yuasa ◽  
Daisuke Neki ◽  
Yasuko Nanjoh ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Drug Resistant ◽  
Fusarium Spp ◽  
Microdilution Method ◽  
In Vitro Antifungal Activity

Abstract In vitro antifungal activity of luliconazole against nondermatophytic moulds causing superficial infections was compared with that of five classes of 12 topical and systemic drugs. The minimum inhibitory concentration (MIC) of the drugs against the genera of Neoscytalidium, Fusarium, Aspergillus, Scedosporium, and Alternaria was measured via modified microdilution method. In results, the nondermatophytic moulds were found to be less susceptible to drugs to which Neoscytalidium spp. and Fusarium spp. were typically drug resistant. However, luliconazole was effective against all the genera tested, including afore-mentioned two species, and had the lowest MICs among the drugs tested.

scholarly journals Potent In Vitro Synergism of Fluconazole and Berberine Chloride against Clinical Isolates of Candida albicans Resistant to Fluconazole

2006 ◽  
Vol 50 (3) ◽  
pp. 1096-1099 ◽  
Author(s):  
Hua Quan ◽  
Ying-Ying Cao ◽  
Zheng Xu ◽  
Jing-Xia Zhao ◽  
Ping-Hui Gao ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Clinical Isolates ◽  
Antagonistic Action ◽  
Berberine Chloride ◽  
Agar Diffusion ◽  
Fungistatic Activity ◽  
Fluconazole Resistant ◽  
Time Kill ◽  
In Vitro Interaction

ABSTRACT In vitro interaction of fluconazole and berberine chloride was investigated against 40 fluconazole-resistant clinical isolates of Candida albicans. Synergism in fungistatic activity was found with the checkerboard microdilution assay. The findings of agar diffusion tests and time-kill curves confirmed the synergistic interaction, but no antagonistic action was observed.

scholarly journals Comparative resistance of Candida albicans clinical isolates to fluconazole and itraconazole in vitro and in vivo in a murine model.

1996 ◽  
Vol 40 (6) ◽  
pp. 1342-1345 ◽  
Author(s):  
A Valentin ◽  
R Le Guennec ◽  
E Rodriguez ◽  
J Reynes ◽  
M Mallie ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Murine Model ◽  
Survival Rates ◽  
Drug Regimen ◽  
Clinical Isolates ◽  
In Vitro Susceptibility ◽  
Microdilution Method ◽  
Broth Microdilution Method

Relationships between azole susceptibility and in vivo response to antifungal therapy in a murine model of candidiasis were investigated for Candida albicans isolates sampled from human immunodeficiency virus type 1-positive patients with oropharyngeal candidiasis. The susceptibilities of seven clinical isolates and two reference strains to fluconazole (FCZ) and itraconazole (ITZ) were determined in vitro by the broth microdilution method. Four isolates were resistant to FCZ and ITZ, two were susceptible to both azoles, and three were resistant to FCZ and susceptible to ITZ (dissociated resistance). CD1 mice were inoculated with each isolate and treated with either FCZ or ITZ (drug regimen, 5 mg/kg of body weight twice daily for 5 days). Quantitative cultures of kidneys were performed at the end of the treatment. On the other hand, the survival rates of the mice were followed daily. These two parameters were clearly correlated with in vitro susceptibility. Thus, the phenomenon of a dissociation of resistance to FCZ and ITZ may be found in vivo as well as in vitro.

scholarly journals Antifungal Activity of Plumericin and Isoplumericin

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Dharmendra Singh ◽  
Umakant Sharma ◽  
Parveen Kumar ◽  
Yogesh K Gupta ◽  
M. P. Dobhal ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Cryptococcus Neoformans ◽  
Inhibitory Concentration ◽  
Chloroform Extract ◽  
Drug Candidate ◽  
Minimum Fungicidal Concentration ◽  
In Vitro Antifungal Activity

This study evaluated the in vitro antifungal activity of the chloroform extract of Plumeria bicolor and its phytoconstituents plumericin and isoplumericin against Candida species and Cryptococcus neoformans by measuring the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC). Plumericin's consistently high activity against Candida albicans, C. krusei, C. glabrata, C. tropicalis and Cryptococcus neoformans was more potent than isoplumericin and the standard antifungal drug nystatin suggesting its potential as a drug candidate for candidiasis and cryptococcosis.

scholarly journals IN VITRO ANTIFUNGAL ACTIVITY OF TIMOL AND CARVACROL ON CANDIDA SPECIES

2019 ◽  
Vol 4 ◽  
Author(s):  
Luciana Thaís Rangel Souza ◽  
Cecília Correia Costa ◽  
Mateus Cardoso Oliveira ◽  
Isabel Celeste Caires Pereira Gusmão
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Candida Krusei ◽  
Minimum Fungicidal Concentration ◽  
Sabouraud Agar ◽  
In Vitro Antifungal Activity

Aim: to evaluate the in vitro action of thymol and carvacrol against the yeasts of Candida albicans ATCC10231 and Candida krusei ATCC34135. Method: A laboratory study was performed to evaluate antifungal activity. The characterization of the Minimal Inhibitory Concentration (MIC) of the thymol essential oil was carried out using the technique where the microdilution is performed, in which a plate containing 96 wells is used. The determination of the Minimum Fungicidal Concentration (MFC) was performed by dripping 10 μL of each of the concentrations evaluated on Sabouraud agar plates. Results: The MIC of thymol and carvacrol for C. albicans was 40 μg/mL and for Candida krusei it did not present antifungal activity. While the MIC of nystatin was 0.03mg for both species with thymol and carvacrol. Conclusion: Thymol presented satisfactory antifungal activity against the pathogens studied, but carvacrol did not present antifungal activity.

scholarly journals Antifungal properties of Foeniculum vulgare, Carum carvi and Eucalyptus sp. essential oils against Candida albicans strains

2013 ◽  
pp. 195-202 ◽  
Author(s):  
Jelica Skrobonja ◽  
Dafina Delic ◽  
Maja Karaman ◽  
Milan Matavulj ◽  
Mirjana Bogavac
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oils ◽  
Inhibition Zone ◽  
Biologically Active ◽  
Foeniculum Vulgare ◽  
Minimum Fungicidal Concentration ◽  
Carum Carvi ◽  
Microdilution Method

Aromatic plants are among the most important sources of biologically active secondary metabolites, with high antimicrobal potential. This study was carried out to examine in vitro antifungal activity of Foeniculum vulgare (Apiaceae), Carum carvi (Apiaceae) and Eucalyptus sp.(Myrtaceae) essential oils against three Candida albicans strains of different origin (laboratory-CAL, human pulmonary-CAH and ATCC10231-CAR). The essential oils were screened on C. albicans using disc and well-diffusion and microdilution method, and compared to Nystatine and Fluconazole as standard anti-mycotics. The activity of tested oils was expressed by inhibition zone diameter (mm), minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) (mg/ml). The results indicated that studied essential oils show antifungal activity against all three isolates of C. albicans. It was observed that each oil exhibits different degree of antifungal activity depending on the oil concentration applied as well as on analyzed strain of C. albicans. Carum carvi demonstrated the strongest antifungal effect to all tested strains, showing the lowest MIC values (0.03mg/ml for CAL, 0.06mg/ml for CAH, and 0.11mg/ml for CAR, respectively). Eucalyptus sp. exhibited the lowest antifungal activity, with MIC values ranging from 0.11 mg/ml for CAL to 0.45 mg/ml for both CAH and CAR.

scholarly journals In vitro study of antifungal effect of carica papaya peel var. California extract against Candida albicans

2021 ◽  
Vol 8 (1) ◽  
pp. 61-68
Author(s):  
Trisnawaty K ◽  
Anin Esta Rauna ◽  
Siti Rusdiana Puspa Dewi ◽  
Pudji Handayani
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Control Group ◽  
Antifungal Effect ◽  
Peel Extract

Candida albicans is a normal microflora in the oral cavity which can be an opportunistic pathogen that could cause oral candidiasis when there are underlying predisposing conditions. Papaya as one of the most widely grown herbal plants in Indonesia has been known to cure diseases. Unused papaya peel only became a waste; however, the papaya peel is known to contain active compounds that have antifungal effect, especially raw ones. This study aimed to determine the antifungal effect of raw papaya peel extract of Californian variety and determine the minimum inhibitory concentration (MIC) of this extract on clinical isolates of C. albicans. This study was an experimental laboratory study with post-test only control group design. Ethanol-based extract of papaya peel was obtained from maceration process using 96% ethanol. Antifungal effect testing was carried out in vitro using disc diffusion method (Kirby-Bauer) on clinical isolates of C. albicans. The concentration of Ethanol extract of papaya peel used consisted of 2.5%, 5%, 10%, and 20% with positive control (nystatin) and negative control (distilled water). The results showed that 2.5%, 5%, 10%, and 20% papaya peel extracts were able to inhibit the growth of C. albicans with 2.5% concentration of raw papaya peel extract as minimum inhibitory concentration. Ethanol extract of raw papaya peel of Californian variety with 2.5%, 5%, 10%, and 20% concentrations has small antifungal effect against clinical isolates of C. albicans.

scholarly journals In VitroEvaluation of the Type of Interaction Obtained by the Combination of Terbinafine and Itraconazole, Voriconazole, or Amphotericin B against Dematiaceous Molds

2011 ◽  
Vol 55 (9) ◽  
pp. 4485-4487 ◽  
Author(s):  
Fernanda Simas Corrêa Biancalana ◽  
Luzia Lyra ◽  
Angélica Zaninelli Schreiber
Keyword(s):  
Amphotericin B ◽  
Alternative Treatment ◽  
Concentration Index ◽  
Inhibitory Concentration ◽  
Drug Combinations ◽  
Fractional Inhibitory Concentration ◽  
Fractional Inhibitory Concentration Index ◽  
Single Drug ◽  
Microdilution Method

ABSTRACTIn vitroassociations using the checkerboard microdilution method indicated lower MIC ranges and MIC median values for each drug (terbinafine, itraconazole, voriconazole, and amphotericin B) in association than those obtained for each single drug. Fractional inhibitory concentration index (FIC) results showed 100% synergism in the association of terbinafine with voriconazole, 96.5% in the association of terbinafine with amphotericin B, and 75.9% in the association of terbinafine with itraconazole. Drug combinations may be useful for treatment of dematiaceous mold infections as an alternative treatment to enhance the effectiveness of each drug.

scholarly journals Antifungal Activity of Amphotericin B Cochleates against Candida albicans Infection in a Mouse Model

2000 ◽  
Vol 44 (6) ◽  
pp. 1463-1469 ◽  
Author(s):  
Leila Zarif ◽  
John R. Graybill ◽  
David Perlin ◽  
Laura Najvar ◽  
Rosie Bocanegra ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Amphotericin B ◽  
Blood Cells ◽  
Inhibitory Concentration ◽  
Human Red Blood Cells ◽  
Unique Shape ◽  
Tissue Burden ◽  
Candida Albicans Infection

ABSTRACT Cochleates are lipid-based supramolecular assemblies composed of natural products, negatively charged phospholipid, and a divalent cation. Cochleates can encapsulate amphotericin B (AmB), an important antifungal drug. AmB cochleates (CAMB) have a unique shape and the ability to target AmB to fungi. The minimal inhibitory concentration and the minimum lethal concentration against Candida albicans are similar to that for desoxycholate AmB (DAMB; Fungizone). In vitro, CAMB induced no hemolysis of human red blood cells at concentrations of as high as 500 μg of AmB/ml, and DAMB was highly hemolytic at 10 μg of AmB/ml. CAMB protect ICR mice infected with C. albicans when the agent is administered intraperitoneally at doses of as low as 0.1 mg/kg/day. In a tissue burden study, CAMB, DAMB, and AmBisome (liposomal AmB; LAMB) were effective in the kidneys, but in the spleen CAMB was more potent than DAMB at 1 mg/kg/day and was equivalent to LAMB at 10 mg/kg/day. In summary, CAMB are highly effective in treating murine candidiasis and compare well with AmBisome and AmB.

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