scholarly journals Microbial Links between Sulfate Reduction and Metal Retention in Uranium- and Heavy Metal-Contaminated Soil

2010 ◽  
Vol 76 (10) ◽  
pp. 3143-3152 ◽  
Author(s):  
Jana Sitte ◽  
Denise M. Akob ◽  
Christian Kaufmann ◽  
Kai Finster ◽  
Dipanjan Banerjee ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Sulfate Reduction ◽  
Contaminated Soil ◽  
Solid Phase ◽  
Metal Mobility ◽  
Mining District ◽  
Edge Structure ◽  
Sulfate Reducing ◽  
Low X

ABSTRACT Sulfate-reducing bacteria (SRB) can affect metal mobility either directly by reductive transformation of metal ions, e.g., uranium, into their insoluble forms or indirectly by formation of metal sulfides. This study evaluated in situ and biostimulated activity of SRB in groundwater-influenced soils from a creek bank contaminated with heavy metals and radionuclides within the former uranium mining district of Ronneburg, Germany. In situ activity of SRB, measured by the 35SO4 2− radiotracer method, was restricted to reduced soil horizons with rates of ≤142 ± 20 nmol cm−3 day−1. Concentrations of heavy metals were enriched in the solid phase of the reduced horizons, whereas pore water concentrations were low. X-ray absorption near-edge structure (XANES) measurements demonstrated that ∼80% of uranium was present as reduced uranium but appeared to occur as a sorbed complex. Soil-based dsrAB clone libraries were dominated by sequences affiliated with members of the Desulfobacterales but also the Desulfovibrionales, Syntrophobacteraceae, and Clostridiales. [13C]acetate- and [13C]lactate-biostimulated soil microcosms were dominated by sulfate and Fe(III) reduction. These processes were associated with enrichment of SRB and Geobacteraceae; enriched SRB were closely related to organisms detected in soils by using the dsrAB marker. Concentrations of soluble nickel, cobalt, and occasionally zinc declined ≤100% during anoxic soil incubations. In contrast to results in other studies, soluble uranium increased in carbon-amended treatments, reaching ≤1,407 nM in solution. Our results suggest that (i) ongoing sulfate reduction in contaminated soil resulted in in situ metal attenuation and (ii) the fate of uranium mobility is not predictable and may lead to downstream contamination of adjacent ecosystems.

Precipitation of heavy metals from coal ash leachate using biogenic hydrogen sulfide generated from FGD gypsum

2013 ◽  
Vol 67 (2) ◽  
pp. 311-318 ◽  
Author(s):  
Madawala Liyanage Duminda Jayaranjan ◽  
Ajit P. Annachhatre
Keyword(s):  
Heavy Metals ◽  
Hydrogen Sulfide ◽  
Sulfate Reduction ◽  
Bottom Ash ◽  
Coal Ash ◽  
Reduction Process ◽  
Fgd Gypsum ◽  
Sulfate Reducing ◽  
Sulfate Reduction Process ◽  
Biological Sulfate Reduction

Investigations were undertaken to utilize flue gas desulfurization (FGD) gypsum for the treatment of leachate from the coal ash (CA) dump sites. Bench-scale investigations consisted of three main steps namely hydrogen sulfide (H2S) production by sulfate reducing bacteria (SRB) using sulfate from solubilized FGD gypsum as the electron acceptor, followed by leaching of heavy metals (HMs) from coal bottom ash (CBA) and subsequent precipitation of HMs using biologically produced sulfide. Leaching tests of CBA carried out at acidic pH revealed the existence of several HMs such as Cd, Cr, Hg, Pb, Mn, Cu, Ni and Zn. Molasses was used as the electron donor for the biological sulfate reduction (BSR) process which produced sulfide rich effluent with concentration up to 150 mg/L. Sulfide rich effluent from the sulfate reduction process was used to precipitate HMs as metal sulfides from CBA leachate. HM removal in the range from 40 to 100% was obtained through sulfide precipitation.

Microbial ecology of sulfate-reducing bacteria in wastewater biofilms analyzed by microelectrodes and fish (fluorescent in situ hybridization) technique

1999 ◽  
Vol 39 (7) ◽  
pp. 41-47 ◽  
Author(s):  
Satoshi Okabe ◽  
Hisashi Satoh ◽  
Tsukasa Itoh ◽  
Yoshimasa Watanabe
Keyword(s):  
In Situ Hybridization ◽  
Sulfate Reduction ◽  
Sulfate Reducing Bacteria ◽  
Hybridization Technique ◽  
Concentration Profiles ◽  
Reduction Zone ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Culture Dependent

The vertical distribution of sulfate-reducing bacteria (SRB) in microaerophilic wastewater biofilms grown on fully submerged rotating disk reactors (RDR) was determined by the conventional culture-dependent MPN method and in situ hybridization of fluorescently-labelled 16S rRNA-targeted oligonucleotide probes for SRB in parallel. Chemical concentration profiles within the biofilm were also measured using microelectrodes for O2, S2-, NO3- and pH. In situ hybridization revealed that the SRB probe-stained cells were distributed throughout the biofilm even in the oxic surface zone in all states from single scattered cells to clustered cells. The higher fluorescence intensity and abundance of SRB probe-stained cells were found in the middle part of the biofilm. This result corresponded well with O2 and H2S concentration profiles measured by microelectrodes, showing sulfate reduction was restricted to a narrow anaerobic zone located about 500 μm below the biofilm surface. Results of the MPN and potential sulfate reducing activity (culture-dependent approaches) indicated a similar distribution of cultivable SRB in the biofilm. The majority of the general SRB probe-stained cells were hybridized with SRB 660 probe, suggesting that one important member of the SRB in the wastewater biofilm could be the genus Desulfobulbus. An addition of nitrate forced the sulfate reduction zone deeper in the biofilm and reduced the specific sulfate reduction rate as well. The sulfate reduction zone was consequently separated from O2 and NO3- respiration zones. Anaerobic H2S oxidation with NO3- was also induced by addition of nitrate to the medium.

scholarly journals Salinity Responses of Benthic Microbial Communities in a Solar Saltern (Eilat, Israel)

2004 ◽  
Vol 70 (3) ◽  
pp. 1608-1616 ◽  
Author(s):  
Ketil Bernt S�rensen ◽  
Donald E. Canfield ◽  
Aharon Oren
Keyword(s):  
Sulfate Reduction ◽  
Reduction Rate ◽  
Sulfate Reduction Rate ◽  
Anoxygenic Phototrophs ◽  
Sulfate Reducing ◽  
Oxygen Budget ◽  
Reducing Bacteria ◽  
Increased Activity ◽  
Optimum Salinity

ABSTRACT The salinity responses of cyanobacteria, anoxygenic phototrophs, sulfate reducers, and methanogens from the laminated endoevaporitic community in the solar salterns of Eilat, Israel, were studied in situ with oxygen microelectrodes and in the laboratory in slurries. The optimum salinity for the sulfate reduction rate in sediment slurries was between 100 and 120‰, and sulfate reduction was strongly inhibited at an in situ salinity of 215‰. Nevertheless, sulfate reduction was an important respiratory process in the crust, and reoxidation of formed sulfide accounted for a major part of the oxygen budget. Methanogens were well adapted to the in situ salinity but contributed little to the anaerobic mineralization in the crust. In slurries with a salinity of 180‰ or less, methanogens were inhibited by increased activity of sulfate-reducing bacteria. Unicellular and filamentous cyanobacteria metabolized at near-optimum rates at the in situ salinity, whereas the optimum salinity for anoxygenic phototrophs was between 100 and 120‰.

scholarly journals Modern environmental technologies of healthy soils contaminated by heavy metals and radionuclides

2020 ◽  
Vol 166 ◽  
pp. 01007
Author(s):  
Vasyl Savosko ◽  
Aleksandr Podolyak ◽  
Irina Komarova ◽  
Aleksey Karpenko
Keyword(s):  
Heavy Metals ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Chemical Elements ◽  
Soil Layer ◽  
Ex Situ ◽  
Soil Restoration ◽  
Mission Strategy ◽  
Environmental Technologies

Object of research: to systematize (taking into account the possible consequences to biosphere) the known technologies for ecological restoration of soils contaminated by heavy metals and radionuclides. Only a healing technology should be recognized as one possible methodology for solving any soil problems. For soils contaminated by heavy metals and radionuclides healing patterns is conceptually ordered into the following levels: mission, strategy, technology. The mission of healthy soil should be aimed at maintaining the chemical elements content within the optimum interval. The strategy of healthy soil involves the regulation of individual elements content in the soil. Ex-situ a soil healing technology is implemented outside the original pollution site. In-situ, a soil healing technology is carried out directly on the original pollution site. Excavation of the contaminated soil layer is the first stage for ex-situ soil restoration. In the future it will be possible: 1) storage of contaminated soil at special landfills, 2) treatment of contaminated soil at a special reactor. All technologies for in-situ healthy of heavy metals contaminated soils can be ordered as: 1) localization, 2) deconcentration, 3) inactivation, 4) extraction.

scholarly journals Diversity decoupled from sulfur isotope fractionation in a sulfate reducing microbial community

2019 ◽  
Author(s):  
Jesse Colangelo ◽  
Claus Pelikan ◽  
Craig W. Herbold ◽  
Ianina Altshuler ◽  
Alexander Loy ◽  
...  
Keyword(s):  
Microbial Community ◽  
Microbial Ecology ◽  
Sulfate Reduction ◽  
Isotope Fractionation ◽  
Sulfur Isotopes ◽  
List Type ◽  
Natural Habitats ◽  
Sulfate Reducing ◽  
Sulfate Reduction Rates

AbstractThe extent of fractionation of sulfur isotopes by sulfate reducing microbes is dictated by genomic and environmental factors. A greater understanding of species-specific fractionations may better inform interpretation of sulfur isotopes preserved in the rock record. To examine whether gene diversity influences net isotopic fractionation in situ, we assessed environmental chemistry, sulfate reduction rates, diversity of putative sulfur metabolizing organisms by 16S rRNA and dissimilatory sulfite reductase (dsrB) gene amplicon sequencing, and net fractionation of sulfur isotopes along a sediment transect of a hypersaline Arctic spring. In situ sulfate reduction rates yielded minimum cell-specific sulfate reduction rates <0.3 x 10−15 moles cell−1 day−1. Neither 16S rRNA nor dsrB diversity indices correlated with relatively constant (38 to 45‰) net isotope fractionation (ε34Ssulfide−sulfate). Measured ε34S values could be reproduced in a mechanistic fractionation model if 1-2% of the microbial community (10-60% of Deltaproteobacteria) were engaged in sulfate respiration, indicating heterogeneous respiratory activity within sulfate-metabolizing populations. This model indicated enzymatic kinetic diversity of Apr was more likely to correlate with sulfur fractionation than DsrB. We propose that, above a threshold alpha diversity value, the influence of the specific composition of the microbial community responsible for generating an isotope signal is overprinted by the control exerted by environmental variables on microbial physiology.Subject categoriesIntegrated genomics and post-genomics approaches in microbial ecologyMicrobial ecology and functional diversity of natural habitats

In situ immobilization of heavy metals associated with uranium leach mines by bacterial sulfate reduction

1996 ◽  
Vol 43 (1-3) ◽  
pp. 231-239 ◽  
Author(s):  
John L. Uhrie ◽  
James I. Drever ◽  
Patricia J.S. Colberg ◽  
Carl C. Nesbitt
Keyword(s):  
Heavy Metals ◽  
Sulfate Reduction ◽  
Bacterial Sulfate Reduction ◽  
In Situ Immobilization

Effect of nitrite and nitrate on biogenic sulfide production in sewer biofilms determined by the use of microelectrodes

2003 ◽  
Vol 47 (11) ◽  
pp. 281-288 ◽  
Author(s):  
S. Okabe ◽  
T. Ito ◽  
H. Satoh ◽  
Y. Watanabe
Keyword(s):  
Sulfate Reduction ◽  
Reduction Rate ◽  
Rotating Disk ◽  
Sulfate Reduction Rate ◽  
Reduction Zone ◽  
Sulfate Reducing ◽  
H2s Oxidation ◽  
Rotating Disk Reactors ◽  
Microelectrode Measurements

The effects of O2 and NO3− concentrations on in situ sulfate reduction and sulfide reoxidation in microaerophilic wastewater biofilms grown on rotating disk reactors were investigated by the use of microelectrodes for O2, S2−, NO3−, NO2−, and pH. Microelectrode measurements showed the vertical microzonation of O2 respiration, NO3− respiration, H2S oxidation and SO42− reduction in the biofilms. The microelectrode measurements indicate that sulfate reducing activity was largely restricted to a narrow anaerobic zone located about 500 μm below the biofilm surface. An addition of nitrate forced the sulfate reduction zone deeper in the biofilm and reduced the specific sulfate reduction rate as well. The sulfate reduction zone was consequently separated from the O2 and NO3− respiration zones. Anaerobic H2S oxidation with NO3− was also induced by addition of nitrate to the medium. Measurements of the reduced inorganic sulfur compounds (FeS, FeS2 and S0), total-Mn and total-Fe in the biofilm indicated that the produced H2S became immediately oxidized with O2, NO3− and other oxidants, mainly ferric/ferrous hydrates. On the basis of the present results, it was estimated that of all sulfide produced, 13% of the sulfide was precipitated by metal ions as FeS and S0 just above the sulfate reduction zone, 65% was anaerobically oxidized to SO42− with NO3− as an electron acceptor and 22% was aerobically oxidized within the biofilm incubated in 70 μmol l−1 of DO and 280 μmol l−1 of NO3−.

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