scholarly journals Microbial Community Dynamics of an Urban Drinking Water Distribution System Subjected to Phases of Chloramination and Chlorination Treatments

2012 ◽  
Vol 78 (22) ◽  
pp. 7856-7865 ◽  
Author(s):  
Chiachi Hwang ◽  
Fangqiong Ling ◽  
Gary L. Andersen ◽  
Mark W. LeChevallier ◽  
Wen-Tso Liu
Keyword(s):  
Drinking Water ◽  
Microbial Community ◽  
Microbial Communities ◽  
Distribution Systems ◽  
Water Distribution ◽  
Microbial Populations ◽  
Rrna Gene ◽  
Source Water ◽  
Content Type ◽  
Drinking Water Distribution

ABSTRACTWater utilities in parts of the U.S. control microbial regrowth in drinking water distribution systems (DWDS) by alternating postdisinfection methods between chlorination and chloramination. To examine how this strategy influences drinking water microbial communities, an urban DWDS (population ≅ 40,000) with groundwater as the source water was studied for approximately 2 years. Water samples were collected at five locations in the network at different seasons and analyzed for their chemical and physical characteristics and for their microbial community composition and structure by examining the 16S rRNA gene via terminal restriction fragment length polymorphism and DNA pyrosequencing technology. Nonmetric multidimension scaling and canonical correspondence analysis of microbial community profiles could explain >57% of the variation. Clustering of samples based on disinfection types (free chlorine versus combined chlorine) and sampling time was observed to correlate to the shifts in microbial communities. Sampling location and water age (<21.2 h) had no apparent effects on the microbial compositions of samples from most time points. Microbial community analysis revealed that among major core populations,Cyanobacteria,Methylobacteriaceae,Sphingomonadaceae, andXanthomonadaceaewere more abundant in chlorinated water, andMethylophilaceae,Methylococcaceae, andPseudomonadaceaewere more abundant in chloraminated water. No correlation was observed with minor populations that were detected frequently (<0.1% of total pyrosequences), which were likely present in source water and survived through the treatment process. Transient microbial populations includingFlavobacteriaceaeandClostridiaceaewere also observed. Overall, reversible shifts in microbial communities were especially pronounced with chloramination, suggesting stronger selection of microbial populations from chloramines than chlorine.

scholarly journals Biofilm formation potential and chlorine resistance of typical bacteria isolated from drinking water distribution systems

RSC Advances ◽  
2020 ◽  
Vol 10 (52) ◽  
pp. 31295-31304 ◽  
Author(s):  
Zebing Zhu ◽  
Lili Shan ◽  
Fengping Hu ◽  
Zehua Li ◽  
Dan Zhong ◽  
...  
Keyword(s):  
Drinking Water ◽  
Microbial Communities ◽  
Biofilm Formation ◽  
Distribution Systems ◽  
Water Distribution ◽  
Water Distribution Systems ◽  
Formation Potential ◽  
Drinking Water Distribution Systems ◽  
Drinking Water Distribution ◽  
Safety Of Drinking Water

Biofilms are the main carrier of microbial communities throughout drinking water distribution systems (DWDSs), and strongly affect the safety of drinking water.

scholarly journals Protozoan Bacterivory and Escherichia coli Survival in Drinking Water Distribution Systems

1998 ◽  
Vol 64 (1) ◽  
pp. 197-202 ◽  
Author(s):  
I. Sibille ◽  
T. Sime-Ngando ◽  
L. Mathieu ◽  
J. C. Block
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Microbial Communities ◽  
Distribution Systems ◽  
Water Distribution ◽  
Water Distribution Systems ◽  
Trophic Relationships ◽  
Bacterial Cells ◽  
Drinking Water Distribution Systems ◽  
Drinking Water Distribution

ABSTRACT The development of bacterial communities in drinking water distribution systems leads to a food chain which supports the growth of macroorganisms incompatible with water quality requirements and esthetics. Nevertheless, very few studies have examined the microbial communities in drinking water distribution systems and their trophic relationships. This study was done to quantify the microbial communities (especially bacteria and protozoa) and obtain direct and indirect proof of protozoan feeding on bacteria in two distribution networks, one of GAC water (i.e., water filtered on granular activated carbon) and the other of nanofiltered water. The nanofiltered water-supplied network contained no organisms larger than bacteria, either in the water phase (on average, 5 × 107bacterial cells liter−1) or in the biofilm (on average, 7 × 106 bacterial cells cm−2). No protozoa were detected in the whole nanofiltered water-supplied network (water plus biofilm). In contrast, the GAC water-supplied network contained bacteria (on average, 3 × 108 cells liter−1 in water and 4 × 107 cells cm−2 in biofilm) and protozoa (on average, 105cells liter−1 in water and 103 cells cm−2 in biofilm). The water contained mostly flagellates (93%), ciliates (1.8%), thecamoebae (1.6%), and naked amoebae (1.1%). The biofilm had only ciliates (52%) and thecamoebae (48%). Only the ciliates at the solid-liquid interface of the GAC water-supplied network had a measurable grazing activity in laboratory test (estimated at 2 bacteria per ciliate per h). Protozoan ingestion of bacteria was indirectly shown by adding Escherichia colito the experimental distribution systems. Unexpectedly, E. coli was lost from the GAC water-supplied network more rapidly than from the nanofiltered water-supplied network, perhaps because of the grazing activity of protozoa in GAC water but not in nanofiltered water. Thus, the GAC water-supplied network contained a functional ecosystem with well-established and structured microbial communities, while the nanofiltered water-supplied system did not. The presence of protozoa in drinking water distribution systems must not be neglected because these populations may regulate the autochthonous and allochthonous bacterial populations.

scholarly journals Using Amplicon Sequencing To Characterize and Monitor Bacterial Diversity in Drinking Water Distribution Systems

2015 ◽  
Vol 81 (18) ◽  
pp. 6463-6473 ◽  
Author(s):  
Jennifer L. A. Shaw ◽  
Paul Monis ◽  
Laura S. Weyrich ◽  
Emma Sawade ◽  
Mary Drikas ◽  
...  
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Water Treatment ◽  
Bacterial Communities ◽  
Distribution Systems ◽  
Water Distribution ◽  
Amplicon Sequencing ◽  
Content Type ◽  
Drinking Water Distribution Systems ◽  
Drinking Water Distribution

ABSTRACTDrinking water assessments use a variety of microbial, physical, and chemical indicators to evaluate water treatment efficiency and product water quality. However, these indicators do not allow the complex biological communities, which can adversely impact the performance of drinking water distribution systems (DWDSs), to be characterized. Entire bacterial communities can be studied quickly and inexpensively using targeted metagenomic amplicon sequencing. Here, amplicon sequencing of the 16S rRNA gene region was performed alongside traditional water quality measures to assess the health, quality, and efficiency of two distinct, full-scale DWDSs: (i) a linear DWDS supplied with unfiltered water subjected to basic disinfection before distribution and (ii) a complex, branching DWDS treated by a four-stage water treatment plant (WTP) prior to disinfection and distribution. In both DWDSs bacterial communities differed significantly after disinfection, demonstrating the effectiveness of both treatment regimes. However, bacterial repopulation occurred further along in the DWDSs, and some end-user samples were more similar to the source water than to the postdisinfection water. Three sample locations appeared to be nitrified, displaying elevated nitrate levels and decreased ammonia levels, and nitrifying bacterial species, such asNitrospira, were detected.Burkholderialeswere abundant in samples containing large amounts of monochloramine, indicating resistance to disinfection. Genera known to contain pathogenic and fecal-associated species were also identified in several locations. From this study, we conclude that metagenomic amplicon sequencing is an informative method to support current compliance-based methods and can be used to reveal bacterial community interactions with the chemical and physical properties of DWDSs.

scholarly journals Microbial Community Profile of a Lead Service Line Removed from a Drinking Water Distribution System

2011 ◽  
Vol 77 (15) ◽  
pp. 5557-5561 ◽  
Author(s):  
Colin White ◽  
Matthew Tancos ◽  
Darren A. Lytle
Keyword(s):  
Drinking Water ◽  
Microbial Community ◽  
Distribution System ◽  
Water Distribution ◽  
Water Distribution System ◽  
Rrna Gene ◽  
Drinking Water Distribution System ◽  
Service Line ◽  
Drinking Water Distribution ◽  
Microbial Community Profile

ABSTRACTA corroded lead service line was removed from a drinking water distribution system, and the microbial community was profiled using 16S rRNA gene techniques. This is the first report of the characterization of a biofilm on the surface of a corroded lead drinking water service line. The majority of phylotypes have been linked to heavy-metal-contaminated environments.

scholarly journals Emerging investigators series: microbial communities in full-scale drinking water distribution systems – a meta-analysis

2016 ◽  
Vol 2 (4) ◽  
pp. 631-644 ◽  
Author(s):  
Quyen M. Bautista-de los Santos ◽  
Joanna L. Schroeder ◽  
Maria C. Sevillano-Rivera ◽  
Rungroch Sungthong ◽  
Umer Z. Ijaz ◽  
...  
Keyword(s):  
Drinking Water ◽  
Distribution Systems ◽  
Water Distribution ◽  
Meta Analysis ◽  
Water Distribution Systems ◽  
16S Rrna Gene Sequencing ◽  
Full Scale ◽  
Rrna Gene ◽  
Drinking Water Distribution Systems ◽  
Drinking Water Distribution

In this study, we co-analyze all available 16S rRNA gene sequencing studies from bulk drinking water samples in full-scale drinking water distribution systems.

scholarly journals Diversity of bacteria and mycobacteria in biofilms of two urban drinking water distribution systems

2012 ◽  
Vol 58 (3) ◽  
pp. 261-270 ◽  
Author(s):  
Ruyin Liu ◽  
Zhisheng Yu ◽  
Hongxun Zhang ◽  
Min Yang ◽  
Baoyou Shi ◽  
...  
Keyword(s):  
Drinking Water ◽  
Distribution Systems ◽  
Water Distribution ◽  
Water Distribution Systems ◽  
Full Scale ◽  
Rrna Gene ◽  
Mycobacterium Species ◽  
Drinking Water Distribution Systems ◽  
Gene Assay ◽  
Drinking Water Distribution

In this study, to give insight into the bacterial diversity of biofilms from full-scale drinking water distribution systems (DWDSs), the bacterial community compositions of biofilms from two urban DWDSs (Guangzhou and Beijing, China) were determined using a 16S rRNA gene library technique. Meanwhile, the occurrence and diversity of mycobacteria were also analyzed by a Mycobacterium -specific hsp gene assay. The biofilms from the full-scale DWDSs have complex bacterial populations. Proteobacteria was the common and predominant group in all biofilm samples, in agreement with previous reports. The community structures of bacteria at the three sites in Guangzhou DWDS were significantly different, despite the similar physicochemical properties of portable water. Some abundant and peculiar bacterial phylotypes were noteworthy, including Methylophilus , Massilia , and Planomicrobium , members of which are rarely found in DWDSs and their roles in DWDS biofilms are still unclear. The diversity of Mycobacterium species in biofilm samples was rather low. Mycobacterium arupense and Mycobacterium gordonae were the primary Mycobacterium species in Guangzhou and Beijing biofilms, respectively, indicating that M. arupense may be more resistant to chloride than M. gordonae.

scholarly journals Ammonia- and Nitrite-Oxidizing Bacterial Communities in a Pilot-Scale Chloraminated Drinking Water Distribution System

2002 ◽  
Vol 68 (1) ◽  
pp. 73-81 ◽  
Author(s):  
John M. Regan ◽  
Gregory W. Harrington ◽  
Daniel R. Noguera
Keyword(s):  
Drinking Water ◽  
Distribution System ◽  
Distribution Systems ◽  
Water Distribution ◽  
Rflp Analysis ◽  
Drinking Water Treatment ◽  
Pilot Scale ◽  
Rrna Gene ◽  
Ammonia Oxidizing Bacteria ◽  
Drinking Water Distribution

ABSTRACT Nitrification in drinking water distribution systems is a common operational problem for many utilities that use chloramines for secondary disinfection. The diversity of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in the distribution systems of a pilot-scale chloraminated drinking water treatment system was characterized using terminal restriction fragment length polymorphism (T-RFLP) analysis and 16S rRNA gene (ribosomal DNA [rDNA]) cloning and sequencing. For ammonia oxidizers, 16S rDNA-targeted T-RFLP indicated the presence of Nitrosomonas in each of the distribution systems, with a considerably smaller peak attributable to Nitrosospira-like AOB. Sequences of AOB amplification products aligned within the Nitrosomonas oligotropha cluster and were closely related to N. oligotropha and Nitrosomonas ureae. The nitrite-oxidizing communities were comprised primarily of Nitrospira, although Nitrobacter was detected in some samples. These results suggest a possible selection of AOB related to N. oligotropha and N. ureae in chloraminated systems and demonstrate the presence of NOB, indicating a biological mechanism for nitrite loss that contributes to a reduction in nitrite-associated chloramine decay.

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