scholarly journals Eukaryotic Viruses in Wastewater Samples from the United States

2009 ◽  
Vol 75 (5) ◽  
pp. 1402-1409 ◽  
Author(s):  
Erin M. Symonds ◽  
Dale W. Griffin ◽  
Mya Breitbart

ABSTRACT Human fecal matter contains a large number of viruses, and current bacterial indicators used for monitoring water quality do not correlate with the presence of pathogenic viruses. Adenoviruses and enteroviruses have often been used to identify fecal pollution in the environment; however, other viruses shed in fecal matter may more accurately detect fecal pollution. The purpose of this study was to develop a baseline understanding of the types of viruses found in raw sewage. PCR was used to detect adenoviruses, enteroviruses, hepatitis B viruses, herpesviruses, morbilliviruses, noroviruses, papillomaviruses, picobirnaviruses, reoviruses, and rotaviruses in raw sewage collected throughout the United States. Adenoviruses and picobirnaviruses were detected in 100% of raw sewage samples and 25% and 33% of final effluent samples, respectively. Enteroviruses and noroviruses were detected in 75% and 58% of raw sewage samples, respectively, and both viral groups were found in 8% of final effluent samples. This study showed that adenoviruses, enteroviruses, noroviruses, and picobirnaviruses are widespread in raw sewage. Since adenoviruses and picobirnaviruses were detected in 100% of raw sewage samples, they are potential markers of fecal contamination. Additionally, this research uncovered previously unknown sequence diversity in human picobirnaviruses. This baseline understanding of viruses in raw sewage will enable educated decisions to be made regarding the use of different viruses in water quality assessments.

2014 ◽  
Vol 59 (6) ◽  
pp. 2150-2165 ◽  
Author(s):  
J. Renée Brooks ◽  
John J. Gibson ◽  
S. Jean Birks ◽  
Marc H. Weber ◽  
Kent D. Rodecap ◽  
...  

Author(s):  
Bruce D. Lindsey ◽  
Marian P. Berndt ◽  
Brian G. Katz ◽  
Ann F. Ardis ◽  
Kenneth A. Skach

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 798
Author(s):  
Samendra P. Sherchan ◽  
Shalina Shahin ◽  
Jeenal Patel ◽  
Lauren M. Ward ◽  
Sarmila Tandukar ◽  
...  

In this study, we investigated the occurrence of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) RNA in primary influent (n = 42), secondary effluent (n = 24) and tertiary treated effluent (n = 34) collected from six wastewater treatment plants (WWTPs A–F) in Virginia (WWTP A), Florida (WWTPs B, C, and D), and Georgia (WWTPs E and F) in the United States during April–July 2020. Of the 100 wastewater samples analyzed, eight (19%) untreated wastewater samples collected from the primary influents contained SARS-CoV-2 RNA as measured by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays. SARS-CoV-2 RNA were detected in influent wastewater samples collected from WWTP A (Virginia), WWTPs E and F (Georgia) and WWTP D (Florida). Secondary and tertiary effluent samples were not positive for SARS-CoV-2 RNA indicating the treatment processes in these WWTPs potentially removed SARS-CoV-2 RNA during the secondary and tertiary treatment processes. However, further studies are needed to understand the log removal values (LRVs) and transmission risks of SARS-CoV-2 RNA through analyzing wastewater samples from a wider range of WWTPs.


Author(s):  
Nancy Langston

By the 1960s, the failures of research and cooperative pragmatism to control Great Lakes pollution were becoming painfully evident. In 1972 Canada and the United States signed the Great Lakes Water Quality Agreement. The agreement was groundbreaking in its focus on cleaning up existing pollution and preventing new pollutants, but the International Joint Commission has no authority to force the two nations to implement recommendations. Therefore, when Canada or the United States refuses to abide by the Great Lakes Water Quality Agreement (in its various revisions), very little happens in response—besides calls for more research.


1985 ◽  
Vol 48 (9) ◽  
pp. 815-823 ◽  
Author(s):  
GARY P. RICHARDS

Outbreaks of hepatitis A, Norwalk illness, and nonspecific viral gastroenteritis are associated with consumption of sewage-contaminated shellfish. Over 100 outbreaks have been reported in the United States during the past 50 years. Reported cases of shellfish-associated enteric virus illness are on the increase, whereas bacterial illness from shellfish is on the decline. As yet, there are no procedures for detecting hepatitis A virus, Norwalk virus and numerous other pathogenic viruses in environmental samples, but virus extraction and assay procedures for water and shellfish are available for the more easily cultivated enteric viruses. Current standards rely on bacterial indicators as a means to evaluate the sanitary quality of shellfish and their growing waters, but the adequacy of using bacteria as indicators of possible virus contamination is questionable. The feasibility of employing enteroviruses or rotaviruses as possible viral indiators is discussed. It is proposed that easily cultivated enteroviruses, such as poliovirus, be used as an interim indicator for the possible presence of human pathogenic viruses in seafoods, with the subsequent formulation of guidelines to limit the levels of virus contamination in shellfish.


2005 ◽  
Vol 52 (9) ◽  
pp. 235-242
Author(s):  
J.G. Schulte ◽  
A.H. Vicory

Source water quality is of major concern to all drinking water utilities. The accidental introduction of contaminants to their source water is a constant threat to utilities withdrawing water from navigable or industrialized rivers. The events of 11 September, 2001 in the United States have heightened concern for drinking water utility security as their source water and finished water may be targets for terrorist acts. Efforts are underway in several parts of the United States to strengthen early warning capabilities. This paper will focus on those efforts in the Ohio River Valley Basin.


Water ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 99 ◽  
Author(s):  
Teresa Cavazos Cohn ◽  
Kate Berry ◽  
Kyle Powys Whyte ◽  
Emma Norman

Hydrosocial spatio-temporalities—aspects of water belonging to space, time, or space-time—are central to water governance, providing a framework upon which overall hydrosocial relations are constructed, and are fundamental to the establishment of values and central to socio-cultural-political relationships. Moreover, spatio-temporal conceptions may differ among diverse governing entities and across scales, creating “variability” through ontological pluralism, as well as power asymmetries embedded in cultural bias. This paper explores spatio-temporal conceptions related to water quality governance, an aspect of water governance often biased toward technical and scientific space-time conceptions. We offer examples of different aspects of spatio-temporality in water quality issues among Tribes in the United States, highlighting several themes, including spatiotemporal cycles, technological mediation, and interrelationship and fluidity. Finally, we suggest that because water is part of a dynamic network of space-times, water quality may be best governed through more holistic practices that recognize tribal sovereignty and hydrosocial variability.


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