scholarly journals PeBL1, a Novel Protein Elicitor from Brevibacillus laterosporus Strain A60, Activates Defense Responses and Systemic Resistance in Nicotiana benthamiana

2015 ◽  
Vol 81 (8) ◽  
pp. 2706-2716 ◽  
Author(s):  
Haoqian Wang ◽  
Xiufen Yang ◽  
Lihua Guo ◽  
Hongmei Zeng ◽  
Dewen Qiu
Keyword(s):  
Pseudomonas Syringae ◽  
Nicotiana Benthamiana ◽  
Fluorescent Protein ◽  
Defense Responses ◽  
Systemic Resistance ◽  
Pcr Analysis ◽  
Content Type ◽  
Brevibacillus Laterosporus ◽  
Protein Elicitor ◽  
Novel Protein

ABSTRACTWe report the identification, characterization, and gene cloning of a novel protein elicitor (PeBL1) secreted fromBrevibacillus laterosporusstrain A60. Through a purification process consisting of ion-exchange chromatography and high-performance liquid chromatography (HPLC), we isolated a protein that was identified by electrospray ionization quadrupole time of flight tandem mass spectrometry (ESI–Q-TOF–MS-MS). The 351-bp PeBL1 gene produces a 12,833-Da protein with 116 amino acids that contains a 30-residue signal peptide. The PeBL1 protein was expressed inEscherichia coli. The recombinant protein can induce a typical hypersensitive response (HR) and systemic resistance inNicotiana benthamiana, like the endogenous protein. PeBL1-treatedN. benthamianaexhibited strong resistance to the infection of tobacco mosaic virus-green fluorescent protein (TMV-GFP) andPseudomonas syringaepv. tabaci compared to controlN. benthamiana. In addition, PeBL1 triggered a cascade of events that resulted in defense responses in plants, including reactive oxygen species (ROS) production, extracellular-medium alkalization, phenolic-compound deposition, and expression of several defense-related genes. Real-time quantitative-PCR analysis indicated that the known defense-related genesPR-1,PR-5,PDF1.2,NPR1, andPALwere upregulated to varying degrees by PeBL1. This research not only provides insights into the mechanism by which beneficial bacteria activate plant systemic resistance, but also sheds new light on a novel strategy for biocontrol using strain A60.

scholarly journals A Novel Protein Elicitor PeBL2, from Brevibacillus laterosporus A60, Induces Systemic Resistance against Botrytis cinerea in Tobacco Plant

2019 ◽  
Vol 35 (3) ◽  
pp. 208-218
Author(s):  
Ghulam Hussain Jatoi ◽  
Guo Lihua ◽  
Yang Xiufen ◽  
Muswar Ali Gadhi ◽  
Azhar Uddin Keerio ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Tobacco Plant ◽  
Systemic Resistance ◽  
Brevibacillus Laterosporus ◽  
Protein Elicitor ◽  
Novel Protein

scholarly journals A Novel Protein Elicitor (PaNie) from Pythium aphanidermatum Induces Multiple Defense Responses in Carrot, Arabidopsis, and Tobacco

2001 ◽  
Vol 127 (3) ◽  
pp. 832-841 ◽  
Author(s):  
Stefan Veit ◽  
Jörg Manfred Wörle ◽  
Thorsten Nürnberger ◽  
Wolfgang Koch ◽  
Hanns Ulrich Seitz
Keyword(s):  
Defense Responses ◽  
Pythium Aphanidermatum ◽  
Protein Elicitor ◽  
Novel Protein

scholarly journals Bacillus cereus AR156 Activates Defense Responses to Pseudomonas syringae pv. tomato in Arabidopsis thaliana Similarly to flg22

2018 ◽  
Vol 31 (3) ◽  
pp. 311-322 ◽  
Author(s):  
Shune Wang ◽  
Ying Zheng ◽  
Chun Gu ◽  
Chan He ◽  
Mengying Yang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Bacillus Cereus ◽  
Pseudomonas Syringae ◽  
Transcriptional Profiling ◽  
Quantitative Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Analysis ◽  
Defense Responses ◽  
Systemic Resistance ◽  
Callose Deposition ◽  
Plant Growth Promoting

Bacillus cereus AR156 (AR156) is a plant growth–promoting rhizobacterium capable of inducing systemic resistance to Pseudomonas syringae pv. tomato in Arabidopsis thaliana. Here, we show that, when applied to Arabidopsis leaves, AR156 acted similarly to flg22, a typical pathogen-associated molecular pattern (PAMP), in initiating PAMP-triggered immunity (PTI). AR156-elicited PTI responses included phosphorylation of MPK3 and MPK6, induction of the expression of defense-related genes PR1, FRK1, WRKY22, and WRKY29, production of reactive oxygen species, and callose deposition. Pretreatment with AR156 still significantly reduced P. syringae pv. tomato multiplication and disease severity in NahG transgenic plants and mutants sid2-2, jar1, etr1, ein2, npr1, and fls2. This suggests that AR156-induced PTI responses require neither salicylic acid, jasmonic acid, and ethylene signaling nor flagella receptor kinase FLS2, the receptor of flg22. On the other hand, AR156 and flg22 acted in concert to differentially regulate a number of AGO1-bound microRNAs that function to mediate PTI. A full-genome transcriptional profiling analysis indicated that AR156 and flg22 activated similar transcriptional programs, coregulating the expression of 117 genes; their concerted regulation of 16 genes was confirmed by real-time quantitative polymerase chain reaction analysis. These results suggest that AR156 activates basal defense responses to P. syringae pv. tomato in Arabidopsis, similarly to flg22.

scholarly journals The Plant Growth–Promoting Rhizobacterium Bacillus cereus AR156 Induces Systemic Resistance in Arabidopsis thaliana by Simultaneously Activating Salicylate- and Jasmonate/Ethylene-Dependent Signaling Pathways

2011 ◽  
Vol 24 (5) ◽  
pp. 533-542 ◽  
Author(s):  
Dong-Dong Niu ◽  
Hong-Xia Liu ◽  
Chun-Hao Jiang ◽  
Yun-Peng Wang ◽  
Qing-Ya Wang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Signaling Pathways ◽  
Pseudomonas Syringae ◽  
Defense Responses ◽  
Systemic Resistance ◽  
Dependent Manner ◽  
Challenge Inoculation ◽  
Plant Growth Promoting ◽  
Growth Promoting

Bacillus cereus AR156 is a plant growth–promoting rhizobacterium that induces resistance against a broad spectrum of pathogens including Pseudomonas syringae pv. tomato DC3000. This study analyzed AR156-induced systemic resistance (ISR) to DC3000 in Arabidopsis ecotype Col-0 plants. Compared with mock-treated plants, AR156-treated ones showed an increase in biomass and reductions in disease severity and pathogen density in the leaves. The defense-related genes PR1, PR2, PR5, and PDF1.2 were concurrently expressed in the leaves of AR156-treated plants, suggesting simultaneous activation of the salicylic acid (SA)- and the jasmonic acid (JA)- and ethylene (ET)-dependent signaling pathways by AR156. The above gene expression was faster and stronger in plants treated with AR156 and inoculated with DC3000 than that in plants only inoculated with DC3000. Moreover, the cellular defense responses hydrogen peroxide accumulation and callose deposition were induced upon challenge inoculation in the leaves of Col-0 plants primed by AR156. Also, pretreatment with AR156 led to a higher level of induced protection against DC3000 in Col-0 than that in the transgenic NahG, the mutant jar1 or etr1, but the protection was absent in the mutant npr1. Therefore, AR156 triggers ISR in Arabidopsis by simultaneously activating the SA- and JA/ET-signaling pathways in an NPR1-dependent manner that leads to an additive effect on the level of induced protection.

scholarly journals Comparative Analysis of Mycobacterial Truncated Hemoglobin Promoters and thegroEL2Promoter in Free-Living and Intracellular Mycobacteria

2012 ◽  
Vol 78 (18) ◽  
pp. 6499-6506 ◽  
Author(s):  
Sunil V. Joseph ◽  
G. K. Madhavilatha ◽  
R. Ajay Kumar ◽  
Sathish Mundayoor
Keyword(s):  
Fluorescent Protein ◽  
Expression Profiles ◽  
Stress Conditions ◽  
Pcr Analysis ◽  
Truncated Hemoglobin ◽  
Oxygen Intermediates ◽  
Free Living ◽  
Content Type ◽  
Time Points

ABSTRACTThe success ofMycobacterium tuberculosisdepends on its ability to withstand and survive the hazardous environment inside the macrophages that are created by reactive oxygen intermediates, reactive nitrogen intermediates, severe hypoxia, low pH, and high CO2levels. Therefore, an effective detoxification system is required for the pathogen to persistin vivo. The genome ofM. tuberculosiscontains a new family of hemoproteins named truncated hemoglobin O (trHbO) and truncated hemoglobin N (trHbN), encoded by theglbOandglbNgenes, respectively, important in the survival ofM. tuberculosisin macrophages. Mycobacterial heat shock proteins are known to undergo rapid upregulation under stress conditions. The expression profiles of the promoters of these genes were studied by constructing transcriptional fusions with green fluorescent protein and monitoring the promoter activity in both free-living and intracellular milieus at different time points. WhereasglbNshowed an early response to the oxidative and nitrosative stresses tested,glbOgave a lasting response to lower concentrations of both stresses. At all time points and under all stress conditions tested,groEL2showed higher expression than both trHb promoters and expression of both promoters showed an increase while inside the macrophages. Real-time PCR analysis of trHb andgroEL2mRNAs showed an initial upregulation at 24 h postinfection. The presence of theglbOprotein imparted an increased survival toM. smegmatisin THP-1 differentiated macrophages compared to that imparted by theglbNandhsp65proteins. The comparative upregulation shown by both trHb promoters while grown inside macrophages indicates the importance of these promoters for the survival ofM. tuberculosisin the hostile environment of the host.

A novel protein elicitor (SsCut) from Sclerotinia sclerotiorum induces multiple defense responses in plants

2014 ◽  
Vol 86 (4-5) ◽  
pp. 495-511 ◽  
Author(s):  
Huajian Zhang ◽  
Qun Wu ◽  
Shun Cao ◽  
Tongyao Zhao ◽  
Ling Chen ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Defense Responses ◽  
Protein Elicitor ◽  
Novel Protein

scholarly journals A Novel Protein Elicitor (PeBA1) from Bacillus amyloliquefaciens NC6 Induces Systemic Resistance in Tobacco

2016 ◽  
Vol 12 (6) ◽  
pp. 757-767 ◽  
Author(s):  
Ningbo Wang ◽  
Mengjie Liu ◽  
Lihua Guo ◽  
Xiufen Yang ◽  
Dewen Qiu
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Systemic Resistance ◽  
Protein Elicitor ◽  
Novel Protein

scholarly journals Suppression of plant defense responses by extracellular metabolites from Pseudomonas syringae pv. tabaci in Nicotiana benthamiana

2013 ◽  
Vol 13 (1) ◽  
pp. 65 ◽  
Author(s):  
Seonghee Lee ◽  
Dong Sik Yang ◽  
Srinivasa Rao Uppalapati ◽  
Lloyd W Sumner ◽  
Kirankumar S Mysore
Keyword(s):  
Plant Defense ◽  
Pseudomonas Syringae ◽  
Nicotiana Benthamiana ◽  
Defense Responses ◽  
Plant Defense Responses ◽  
Extracellular Metabolites

scholarly journals Arabidopsis UGT76B1 glycosylates N-hydroxy-pipecolic acid and inactivates systemic acquired resistance in tomato

2020 ◽  
Author(s):  
Eric C. Holmes ◽  
Yun-Chu Chen ◽  
Mary Beth Mudgett ◽  
Elizabeth S. Sattely
Keyword(s):  
Pseudomonas Syringae ◽  
Systemic Acquired Resistance ◽  
Acquired Resistance ◽  
Bacterial Pathogen ◽  
Defense Responses ◽  
Pipecolic Acid ◽  
Systemic Resistance ◽  
Biosynthetic Genes ◽  
Solanaceous Plants

AbstractSystemic acquired resistance (SAR) is a mechanism that plants utilize to connect a local pathogen infection to global defense responses. N-hydroxy-pipecolic acid (NHP) and a glycosylated derivative are produced during SAR, yet their individual roles in the response have not yet been elucidated. Here we report that Arabidopsis thaliana UGT76B1 can generate glycosylated NHP (NHP-Glc) in vitro and when transiently expressed alongside Arabidopsis NHP biosynthetic genes in two Solanaceous plants. During infection, Arabidopsis ugt76b1 mutants do not accumulate NHP-Glc and accumulate less glycosylated salicylic acid (SA-Glc) than wild type plants. The metabolic changes in ugt76b1 mutant plants are accompanied by enhanced defense to the bacterial pathogen Pseudomonas syringae, suggesting that glycosylation of SAR molecules NHP and SA by UGT76B1 plays an important role in defense modulation. Transient expression of Arabidopsis UGT76B1 with the Arabidopsis NHP biosynthesis genes ALD1 and FMO1 in tomato increases NHP-Glc production and reduces NHP accumulation in local tissue, and abolishes the systemic resistance seen when expressing NHP-biosynthetic genes alone. These findings reveal that the glycosylation of NHP by UGT76B1 alters defense priming in systemic tissue and provide further evidence for the role of the NHP aglycone as the active metabolite in SAR signaling.

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