scholarly journals The Plant Growth–Promoting Rhizobacterium Bacillus cereus AR156 Induces Systemic Resistance in Arabidopsis thaliana by Simultaneously Activating Salicylate- and Jasmonate/Ethylene-Dependent Signaling Pathways

2011 ◽  
Vol 24 (5) ◽  
pp. 533-542 ◽  
Author(s):  
Dong-Dong Niu ◽  
Hong-Xia Liu ◽  
Chun-Hao Jiang ◽  
Yun-Peng Wang ◽  
Qing-Ya Wang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Signaling Pathways ◽  
Pseudomonas Syringae ◽  
Defense Responses ◽  
Systemic Resistance ◽  
Dependent Manner ◽  
Challenge Inoculation ◽  
Plant Growth Promoting ◽  
Growth Promoting

Bacillus cereus AR156 is a plant growth–promoting rhizobacterium that induces resistance against a broad spectrum of pathogens including Pseudomonas syringae pv. tomato DC3000. This study analyzed AR156-induced systemic resistance (ISR) to DC3000 in Arabidopsis ecotype Col-0 plants. Compared with mock-treated plants, AR156-treated ones showed an increase in biomass and reductions in disease severity and pathogen density in the leaves. The defense-related genes PR1, PR2, PR5, and PDF1.2 were concurrently expressed in the leaves of AR156-treated plants, suggesting simultaneous activation of the salicylic acid (SA)- and the jasmonic acid (JA)- and ethylene (ET)-dependent signaling pathways by AR156. The above gene expression was faster and stronger in plants treated with AR156 and inoculated with DC3000 than that in plants only inoculated with DC3000. Moreover, the cellular defense responses hydrogen peroxide accumulation and callose deposition were induced upon challenge inoculation in the leaves of Col-0 plants primed by AR156. Also, pretreatment with AR156 led to a higher level of induced protection against DC3000 in Col-0 than that in the transgenic NahG, the mutant jar1 or etr1, but the protection was absent in the mutant npr1. Therefore, AR156 triggers ISR in Arabidopsis by simultaneously activating the SA- and JA/ET-signaling pathways in an NPR1-dependent manner that leads to an additive effect on the level of induced protection.

scholarly journals Bacillus cereus AR156 Activates Defense Responses to Pseudomonas syringae pv. tomato in Arabidopsis thaliana Similarly to flg22

2018 ◽  
Vol 31 (3) ◽  
pp. 311-322 ◽  
Author(s):  
Shune Wang ◽  
Ying Zheng ◽  
Chun Gu ◽  
Chan He ◽  
Mengying Yang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Bacillus Cereus ◽  
Pseudomonas Syringae ◽  
Transcriptional Profiling ◽  
Quantitative Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Analysis ◽  
Defense Responses ◽  
Systemic Resistance ◽  
Callose Deposition ◽  
Plant Growth Promoting

Bacillus cereus AR156 (AR156) is a plant growth–promoting rhizobacterium capable of inducing systemic resistance to Pseudomonas syringae pv. tomato in Arabidopsis thaliana. Here, we show that, when applied to Arabidopsis leaves, AR156 acted similarly to flg22, a typical pathogen-associated molecular pattern (PAMP), in initiating PAMP-triggered immunity (PTI). AR156-elicited PTI responses included phosphorylation of MPK3 and MPK6, induction of the expression of defense-related genes PR1, FRK1, WRKY22, and WRKY29, production of reactive oxygen species, and callose deposition. Pretreatment with AR156 still significantly reduced P. syringae pv. tomato multiplication and disease severity in NahG transgenic plants and mutants sid2-2, jar1, etr1, ein2, npr1, and fls2. This suggests that AR156-induced PTI responses require neither salicylic acid, jasmonic acid, and ethylene signaling nor flagella receptor kinase FLS2, the receptor of flg22. On the other hand, AR156 and flg22 acted in concert to differentially regulate a number of AGO1-bound microRNAs that function to mediate PTI. A full-genome transcriptional profiling analysis indicated that AR156 and flg22 activated similar transcriptional programs, coregulating the expression of 117 genes; their concerted regulation of 16 genes was confirmed by real-time quantitative polymerase chain reaction analysis. These results suggest that AR156 activates basal defense responses to P. syringae pv. tomato in Arabidopsis, similarly to flg22.

scholarly journals Exploring Elicitors of the Beneficial Rhizobacterium Bacillus amyloliquefaciens SQR9 to Induce Plant Systemic Resistance and Their Interactions With Plant Signaling Pathways

2018 ◽  
Vol 31 (5) ◽  
pp. 560-567 ◽  
Author(s):  
Gengwei Wu ◽  
Yunpeng Liu ◽  
Yu Xu ◽  
Guishan Zhang ◽  
Qirong Shen ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Pseudomonas Syringae ◽  
Bacillus Amyloliquefaciens ◽  
Systemic Resistance ◽  
Plant Signaling ◽  
Relative Contribution ◽  
Induced Plant Resistance ◽  
Plant Growth Promoting ◽  
Induction Of Resistance ◽  
Growth Promoting

Beneficial rhizobacteria have been reported to produce various elicitors that induce plant systemic resistance, but there is little knowledge concerning the relative contribution of multiple elicitors from a single beneficial rhizobacterium on the induced systemic resistance in plants and the interactions of these elicitors with plant signaling pathways. In this study, nine mutants of the plant growth–promoting rhizobacterium Bacillus amyloliquefaciens SQR9 deficient in producing the extracellular compounds, including fengycin, bacillomycin D, surfactin, bacillaene, macrolactin, difficidin, bacilysin, 2,3-butandiol, and exopolysaccharides, were tested for the induction of systemic resistance against Pseudomonas syringae pv. tomato DC3000 and Botrytis cinerea and the transcription of the salicylic acid, jasmonic acid, and ethylene signaling pathways in Arabidopsis. Deficiency in producing any of these compounds in SQR9 significantly weakened the induced plant resistance against these phytopathogens. These SQR9-produced elicitors induced different plant defense genes. For instance, the enhancement of 1,3-glucanase (PR2) by SQR9 was impaired by a deficiency of macrolactin but not surfactin. SQR9 mutants deficient in the lipopeptide and polyketide antibiotics remained only 20% functional for the induction of resistance-related gene transcription. Overall, these elicitors of SQR9 could act synergistically to induce plant systemic resistance against different phytopathogens through different signaling pathway genes, and the bacterial antibiotics are major contributors to the induction.

scholarly journals Isolation, Screening and Response of Maize to Plant Growth Promoting Rhizobacteria Inoculants

2019 ◽  
Vol 50 (3) ◽  
pp. 181-190
Author(s):  
A.K. Akintokun ◽  
E. Ezaka ◽  
P.O. Akintokun ◽  
O.B. Shittu ◽  
L. B. Taiwo
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Pseudomonas Syringae ◽  
Root Length ◽  
Plant Growth Promoting Rhizobacteria ◽  
Alcaligenes Faecalis ◽  
Bacillus Mojavensis ◽  
Significant Difference ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract The use of plant growth promoting rhizobacteria (PGPR) as biofertilizer is a relatively safer, environment friendly and cost effective. This work was designed to assess plant growth promoting abilities of rhizobacteria and evaluate their effect on germination and growth of maize. The bacteria were isolated and screened for plant growth promoting abilities using Pikovskaya agar, Aleksandrov agar and Jensen media. Twelve isolates that showed multiple attributes were further screened for indole acetic acid (IAA) and gibberellic acid (GA) production; best five isolates were selected for further studies. The results of IAA and GA production showed a considerable amount of IAA and GA produced by the isolates which ranged between 9–94 and 21–97 mg l–1, respectively. The selected isolates identified as Bacillusmojavensis, Pseudomonas aeruginosa, Alcaligenes faecalis, Pseudomonas syringae and Bacillus cereus showed a significant difference(P≤0.05) in the amount of potassium(K) and phosphorus(P) solubilized at different source of K (KCl and mica powder) and P (Ca2PO4 and rock phosphate). The isolates also recorded significant level of nitrogen fixing ability with Alcaligenes faecalis strain P156 fixing the highest amount of nitrogen (11.4 mg N fixed per l) and least by Bacillus mojavensis strain NBSL51 (6.3 mg N fixed per l).The results of plant inoculation test showed that Bacillus cereus strain 20UPMNR significantly enhanced the root and shoot dry weight. All the selected isolates enhanced shoot and root length except Bacillus mojavensis which produced less effect on root length when compared to the control. These results have provided vital information for the development of a bio fertilizer for maize.

scholarly journals Protection Against Pathogen and Salt Stress by Four Plant Growth-Promoting Rhizobacteria Isolated from Pinus sp. on Arabidopsis thaliana

2008 ◽  
Vol 98 (6) ◽  
pp. 666-672 ◽  
Author(s):  
J. Barriuso ◽  
B. Ramos Solano ◽  
F. J. Gutiérrez Mañero
Keyword(s):  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Plant Growth ◽  
Pseudomonas Syringae ◽  
Plant Growth Promoting Rhizobacteria ◽  
Systemic Resistance ◽  
Induce Systemic Resistance ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Dependent Pathway

The ability of four plant growth-promoting rhizobacteria, isolated in a previous study, to induce systemic resistance on Arabidopsis thaliana Col 0 against biotic and abiotic stress was evaluated. All the bacteria enhanced protection against the foliar pathogen Pseudomonas syringae DC3000 and increased plant tolerance to salt stress (NaCl 60 mM). Bacillus sp. strain L81 and Arthrobacter oxidans strain BB1 performed best with a decrease in the disease index of 61.2 and 52.3%, respectively, and a reduction in the mortality due to salt stress of 72.4 and 57.8%, respectively. Additionally, significant differences were found in growth and photosynthesis, again, L81 and BB1 performed best either in normal or under stress conditions. In order to elucidate the pathway elicited by these two strains to induce systemic resistance, experiments with the transgenic line of Arabidopsis thaliana NahG (defective in salicylic acid [SA]) and with the jar1 mutant (defective in jasmonic acid) were carried out. Results showed that the SA-dependent pathway was involved in the defense response induced by strains L81 and BB1. Results from quantitative reverse transcription-polymerase chain reaction analysis of the PR1 gene, related to the SA-dependent pathway and the PDF1.2 gene related to the SA-independent pathway, showed an increased expression of PR1 in BB1-treated plants, confirming involvement of the SA-dependent pathway in the defensive response.

Film coating of seeds with Bacillus cereus RS87 spores for early plant growth enhancement

2008 ◽  
Vol 54 (10) ◽  
pp. 861-867 ◽  
Author(s):  
Kanchalee Jetiyanon ◽  
Sakchai Wittaya-Areekul ◽  
Pinyupa Plianbangchang
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Plant Height ◽  
Root Length ◽  
Film Coating ◽  
Field Trials ◽  
Growth Enhancement ◽  
Vegetative Cells ◽  
Plant Growth Promoting ◽  
Growth Promoting

The plant growth-promoting rhizobacterium Bacillus cereus RS87 was previously reported to promote plant growth in various crops in both greenhouse and field trials. To apply as a plant growth promoting agent with practical use, it is essential to ease the burden of routine preparation of a fresh suspension of strain RS87 in laboratory. The objectives of this study were to investigate the feasibility of film-coating seeds with B. cereus RS87 spores for early plant growth enhancement and to reveal the indoleacetic acid (IAA) production released from strain RS87. The experiment consisted of the following 5 treatments: nontreated seeds, water-soaked seeds, film-coated seeds, seeds soaked with vegetative cells of strain RS87, and film-coated seeds with strain RS87 spores. Three experiments were conducted separately to assess seed emergence, root length, and plant height. Results showed that both vegetative cells and spores of strain RS87 significantly promoted (P ≤ 0.05) seed emergence, root length and plant height over the control treatments. The strain RS87 also produced IAA. In conclusion, the film coating of seeds with spores of B. cereus RS87 demonstrated early plant growth enhancement as well as seeds using their vegetative cells. IAA released from strain RS87 would be one of the mechanisms for plant growth enhancement.

Plant growth promoting strain Bacillus cereus (RCS-4 MZ520573.1) enhances phytoremediation potential of Cynodon dactylon L. in distillery sludge

2022 ◽  
pp. 112709
Author(s):  
Sonam Tripathi ◽  
Sangeeta Yadav ◽  
Pooja Sharma ◽  
Diane Purchase ◽  
Asad Syed ◽  
...  
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Cynodon Dactylon ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Plant growth-promoting rhizobacteria induced resistance in Jatropha curcas through phenyl propanoid metabolism against Rhizoctoniabataticola

2017 ◽  
Vol 9 (1) ◽  
pp. 121-128
Author(s):  
S. Kumar ◽  
M. Singh ◽  
Sushil Sharma
Keyword(s):  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Jatropha Curcas ◽  
Root Rot ◽  
Plant Growth Promoting Rhizobacteria ◽  
Systemic Resistance ◽  
Total Phenols ◽  
Phenyl Propanoid ◽  
Plant Growth Promoting ◽  
Growth Promoting

The root rot disease in Jatropha curcas L. caused by Rhizoctonia. bataticola (Taub.) Butler has been recorded in causing 10-12 per cent mortality of 20-30 days old seedlings of Jatropha curcasin southern Haryana. The incidence of this disease has also been observed from other parts of Haryana too. Induction of systemic resistance in host plants through microbes and their bioactive metabolites are attaining popularity in modern agricultural practices. Studies on the plant growth-promoting rhizobacteria induced resistance in Jatropha curcas through phenyl propanoid metabolism against Rhizoctoniabataticola were undertaken at Chaudhary Charan Singh, Haryana Agricultural University, Regional Research Station, Bawal. Three plant growth-promoting rhizobacteria (PGPRs) viz., Pseudomonas maltophila, Pseudomonas fluorescens and Bacillus subtilis were evaluated for their potential to induce systemic resistance in Jatropha against root rot. The maximum increase of 97 per cent in total phenols, 120 per cent in peroxidase, 123 per cent in polyphenol oxidase, 101 per cent in phenylalanine ammonia lyase and 298 per cent in tyrosine ammonia lyase was detected in plants raised with Pseudomonas fluorescens+ Rhizoctoniaba-taticola inoculation in Jatropha curcas at 10 days post inoculation against control except total phenols where it was maximum (99%) at 30 DPI. There was slight or sharp decline in these parameters with age irrespective of inoculations. The pathogen challenged plants showed lower levels of total phenols and enzymes. The observations revealed that seed bacterization with Pseudomonas fluorescens results in accumulation of phenolics and battery of enzymes in response to pathogen infection and thereby induce resistance systemically.

scholarly journals Efficacy of Plant Growth-Promoting Bacteria Bacillus cereus YN917 for Biocontrol of Rice Blast

2021 ◽  
Vol 12 ◽  
Author(s):  
Hu Zhou ◽  
Zuo-hua Ren ◽  
Xue Zu ◽  
Xi-yue Yu ◽  
Hua-jun Zhu ◽  
...  
Keyword(s):  
Disease Prevention ◽  
Plant Growth ◽  
Bacillus Cereus ◽  
Rice Blast ◽  
Growth Promotion ◽  
Gene Clusters ◽  
Plant Growth Promoting Bacteria ◽  
Detached Leaf ◽  
Plant Growth Promoting ◽  
Growth Promoting

Bacillus cereus YN917, obtained from a rice leaf with remarkable antifungal activity against Magnaporthe oryzae, was reported in our previous study. The present study deciphered the possible biocontrol properties. YN917 strain exhibits multiple plant growth-promoting and disease prevention traits, including production of indole-3-acetic acid (IAA), ACC deaminase, siderophores, protease, amylase, cellulase, and β-1,3-glucanase, and harboring mineral phosphate decomposition activity. The effects of the strain YN917 on growth promotion and disease prevention were further evaluated under detached leaf and greenhouse conditions. The results revealed that B. cereus YN917 can promote seed germination and seedling plant growth. The growth status of rice plants was measured from the aspects of rice plumule, radicle lengths, plant height, stem width, root lengths, fresh weights, dry weights, and root activity when YN917 was used as inoculants. YN917 significantly reduced rice blast severity under detached leaf and greenhouse conditions. Genome analysis revealed the presence of gene clusters for biosynthesis of plant promotion and antifungal compounds, such as IAA, tryptophan, siderophores, and phenazine. In summary, YN917 can not only be used as biocontrol agents to minimize the use of chemical substances in rice blast control, but also can be developed as bio-fertilizers to promote the rice plant growth.

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