scholarly journals Abundance, Dynamics, and Biogeographic Distribution of Seven Polycyclic Aromatic Hydrocarbon Dioxygenase Gene Variants in Coastal Sediments of Patagonia

2012 ◽  
Vol 78 (5) ◽  
pp. 1589-1592 ◽  
Author(s):  
Magalí S. Marcos ◽  
Mariana Lozada ◽  
Walter D. Di Marzio ◽  
Hebe M. Dionisi
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Marine Environment ◽  
Time Span ◽  
Coastal Sediments ◽  
Gene Variants ◽  
Content Type ◽  
Coastal Marine ◽  
Polycyclic Aromatic ◽  
Dioxygenase Gene

ABSTRACTNovel polycyclic aromatic hydrocarbon dioxygenase gene variants were present in abundances similar to or higher than those ofphnA1fromCycloclasticusspp. at a chronically polluted subantarctic coastal marine environment in Patagonia. These novel gene variants were detected over a 6-year time span and were also present in sediments from temperate Patagonian sites.

Polycyclic aromatic hydrocarbon analysis in different matrices of the marine environment

2005 ◽  
Vol 547 (2) ◽  
pp. 243-254 ◽  
Author(s):  
Anna Filipkowska ◽  
Ludwik Lubecki ◽  
Grażyna Kowalewska
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Marine Environment ◽  
Hydrocarbon Analysis ◽  
Polycyclic Aromatic

scholarly journals Heterologous Expression of Polycyclic Aromatic Hydrocarbon Ring-Hydroxylating Dioxygenase Genes from a Novel Pyrene-Degrading Betaproteobacterium

2012 ◽  
Vol 78 (10) ◽  
pp. 3552-3559 ◽  
Author(s):  
David R. Singleton ◽  
Jing Hu ◽  
Michael D. Aitken
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Enrichment Culture ◽  
Metagenomic Library ◽  
Stable Isotope Probing ◽  
Content Type ◽  
Close Proximity ◽  
Polycyclic Aromatic ◽  
Dominant Member ◽  
Pah Metabolism

ABSTRACTA betaproteobacterium within the familyRhodocyclaceaepreviously identified as a pyrene degrader via stable-isotope probing (SIP) of contaminated soil (designated pyrene group 1 or PG1) was cultivated as the dominant member of a mixed bacterial culture. A metagenomic library was constructed, and the largest contigs were analyzed for genes associated with polycyclic aromatic hydrocarbon (PAH) metabolism. Eight pairs of genes with similarity to the α- and β-subunits of ring-hydroxylating dioxygenases (RHDs) associated with aerobic bacterial PAH degradation were identified and linked to PG1 through PCR analyses of a simplified enrichment culture. In tandem with a ferredoxin and reductase found in close proximity to one pair of RHD genes, six of the RHDs were cloned and expressed inEscherichia coli. Each cloned RHD was tested for activity against nine PAHs ranging in size from two to five rings. Despite differences in their predicted protein sequences, each of the six RHDs was capable of transforming phenanthrene and pyrene. Three RHDs could additionally transform naphthalene and fluorene, and these genotypes were also associated with the ability of theE. coliconstructs to convert indole to indigo. Only one of the six cloned RHDs was capable of transforming anthracene and benz[a]anthracene. None of the tested RHDs were capable of significantly transforming fluoranthene, chrysene, or benzo[a]pyrene.

scholarly journals Complete Genome Sequence of Pseudomonas putida BS3701, a Promising Polycyclic Aromatic Hydrocarbon-Degrading Strain for Bioremediation Technologies

2020 ◽  
Vol 9 (40) ◽  
Author(s):  
Andrey Filonov ◽  
Yanina Delegan ◽  
Irina Puntus ◽  
Leonid Valentovich ◽  
Artur Akhremchuk ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Pseudomonas Putida ◽  
Russian Federation ◽  
Aromatic Hydrocarbons ◽  
Complete Genome Sequence ◽  
Circular Chromosome ◽  
Content Type ◽  
Polycyclic Aromatic

ABSTRACT The strain Pseudomonas putida BS3701 was isolated from soil contaminated with coke by-product waste (Moscow Region, Russian Federation). It is capable of degrading crude oil and polycyclic aromatic hydrocarbons (PAHs). The P. putida BS3701 genome consists of a 6,337,358-bp circular chromosome and two circular plasmids (pBS1141 with 107,388 bp and pBS1142 with 54,501 bp).

scholarly journals Complete Genome Sequence of the Pyrene-Degrading Bacterium Cycloclasticus sp. Strain P1

2012 ◽  
Vol 194 (23) ◽  
pp. 6677-6677 ◽  
Author(s):  
Qiliang Lai ◽  
Weiwei Li ◽  
Baojiang Wang ◽  
Zhiwei Yu ◽  
Zongze Shao
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Complete Genome Sequence ◽  
Deep Sea ◽  
Complete Genome ◽  
Content Type ◽  
Degrading Bacterium ◽  
The Pacific ◽  
Polycyclic Aromatic ◽  
The Pacific Ocean

ABSTRACTCycloclasticussp. strain P1 was isolated from deep-sea sediments of the Pacific Ocean and characterized as a unique bacterium in the degradation of pyrene, a four-ring polycyclic aromatic hydrocarbon (PAH). Here we report the complete genome of P1 and genes associated with PAH degradation.

scholarly journals Draft Genome Sequence of Marinobacter hydrocarbonoclasticus Strain STW2, a Polycyclic Aromatic Hydrocarbon-Degrading and Denitrifying Bacterium from the Rhizosphere of Seagrass Enhalus acodoides

2017 ◽  
Vol 5 (8) ◽  
Author(s):  
Juan Ling ◽  
Liyun Lin ◽  
Yanying Zhang ◽  
Xiancheng Lin ◽  
Manzoor Ahamad ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Marine Microbes ◽  
Content Type ◽  
Future Studies ◽  
Polycyclic Aromatic ◽  
Marinobacter Hydrocarbonoclasticus

ABSTRACT Here, we report the draft genome sequence of Marinobacter hydrocarbonoclasticus strain STW2, which was isolated from the rhizosphere of seagrass Enhalus acodoides. This study will facilitate future studies on the genetic pathways of marine microbes capable of both polycyclic aromatic hydrocarbon degradation and nitrate reduction.

Distribution and sources of polycyclic aromatic hydrocarbon (PAH) in marine environment of China

2004 ◽  
Vol 22 (2) ◽  
pp. 136-145 ◽  
Author(s):  
Zheng Jinshu ◽  
Bruce J. Richardson ◽  
O. Shouming ◽  
Zheng Jianhua
Keyword(s):  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Marine Environment ◽  
Polycyclic Aromatic

scholarly journals Multiple Roles for Two Efflux Pumps in the Polycyclic Aromatic Hydrocarbon-Degrading Pseudomonas putida Strain B6-2 (DSM 28064)

2017 ◽  
Vol 83 (24) ◽  
Author(s):  
Xuemei Yao ◽  
Fei Tao ◽  
Kunzhi Zhang ◽  
Hongzhi Tang ◽  
Ping Xu
Keyword(s):  
Antibiotic Resistance ◽  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Pseudomonas Putida ◽  
Mutant Strain ◽  
Efflux Pump ◽  
Efflux Pumps ◽  
Content Type ◽  
Pah Degradation ◽  
Polycyclic Aromatic

ABSTRACTMicrobial bioremediation is a promising approach for the removal of polycyclic aromatic hydrocarbon (PAH) contaminants. Many degraders of PAHs possess efflux pump genes in their genomes; however, their specific roles in the degradation of PAHs have not been clearly elucidated. In this study, two efflux pumps, TtgABC and SrpABC, were systematically investigated to determine their functions in a PAH-degradingPseudomonas putidastrain B6-2 (DSM 28064). The disruption of genesttgABCorsrpABCresulted in a defect in organic solvent tolerance. TtgABC was found to contribute to antibiotic resistance; SrpABC only contributed to antibiotic resistance under an artificial overproduced condition. Moreover, a mutant strain withoutsrpABCdid not maintain its activity in long-term biphenyl (BP) degradation, which correlated with the loss of cell viability. The expression of SrpABC was significantly upregulated in the course of BP degradation. BP, 2-hydroxybiphenyl, 3-hydroxybiphenyl, and 2,3-dihydroxybiphenyl (2,3-DHBP) were revealed to be the inducers ofsrpABC. 2,3-DHBP was verified to be a substrate of pump SrpABC; SrpABC can enhance the tolerance to 2,3-DHBP by pumping it out. The mutant strain B6-2ΔsrpSprolonged BP degradation with the increase ofsrpABCexpression. These results suggest that the pump SrpABC of strain B6-2 plays a positive role in BP biodegradation by pumping out metabolized toxic substances such as 2,3-DHBP. This study provides insights into the versatile physiological functions of the widely distributed efflux pumps in the biodegradation of PAHs.IMPORTANCEPolycyclic aromatic hydrocarbons (PAHs) are notorious for their recalcitrance to degradation in the environment. A high frequency of the occurrence of the efflux pump genes was observed in the genomes of effective PAH degraders; however, their specific roles in the degradation of PAHs are still obscure. The significance of our study is in the identification of the function and mechanism of the efflux pump SrpABC ofPseudomonas putidastrain B6-2 (DSM 28064) in the biphenyl degradation process. SrpABC is crucial for releasing the toxicity caused by intermediates that are unavoidably produced in PAH degradation, which enables an understanding of how cells maintain the intracellular balance of materials. The findings from this study provide a new perspective on PAH recalcitrance and shed light on enhancing PAH degradation by genetic engineering.

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