Methanotrophs and Methanogens in Masonry

1998 ◽  
Vol 64 (11) ◽  
pp. 4530-4532 ◽  
Author(s):  
Martin Kussmaul ◽  
Markus Wilimzig ◽  
Eberhard Bock

ABSTRACT Methanotrophs were present in 48 of 225 stone samples which were removed from 19 historical buildings in Germany and Italy. The average cell number of methanotrophs was 20 CFU per g of stone, and their activities ranged between 11 and 42 pmol of CH4 g of stone−1 day−1. Twelve strains of methane-oxidizing bacteria were isolated. They belonged to the type II methanotrophs of the genera Methylocystis,Methylosinus, and Methylobacterium. In masonry, growth substrates like methane or methanol are available in very low concentrations. To determine if methane could be produced by the stone at rates sufficient to support growth of methanotrophs, methane production by stone samples under nonoxic conditions was examined. Methane production of 0.07 to 215 nmol of CH4 g of stone−1 day−1 was detected in 23 of 47 stone samples examined. This indicated the presence of the so-called “mini-methane”-producing bacteria and/or methanogenic archaea. Methanotrophs occurred in nearly all samples which showed methane production. This finding indicated that methanotrophs depend on biogenic methane production in or on stone surfaces of historical buildings.

Author(s):  
T.A. Fassel ◽  
M.J. Schaller ◽  
M.E. Lidstrom ◽  
C.C. Remsen

Methylotrophic bacteria play an Important role in the environment in the oxidation of methane and methanol. Extensive intracytoplasmic membranes (ICM) have been associated with the oxidation processes in methylotrophs and chemolithotrophic bacteria. Classification on the basis of ICM arrangement distinguishes 2 types of methylotrophs. Bundles or vesicular stacks of ICM located away from the cytoplasmic membrane and extending into the cytoplasm are present in Type I methylotrophs. In Type II methylotrophs, the ICM form pairs of peripheral membranes located parallel to the cytoplasmic membrane. Complex cell wall structures of tightly packed cup-shaped subunits have been described in strains of marine and freshwater phototrophic sulfur bacteria and several strains of methane oxidizing bacteria. We examined the ultrastructure of the methylotrophs with particular view of the ICM and surface structural features, between representatives of the Type I Methylomonas albus (BG8), and Type II Methylosinus trichosporium (OB-36).


2020 ◽  
Vol 401 (12) ◽  
pp. 1469-1477
Author(s):  
Anna Hakobyan ◽  
Werner Liesack

AbstractAerobic methane-oxidizing bacteria, or methanotrophs, play a crucial role in the global methane cycle. Their methane oxidation activity in various environmental settings has a great mitigation effect on global climate change. Alphaproteobacterial methanotrophs were among the first to be taxonomically characterized, nowadays unified in the Methylocystaceae and Beijerinckiaceae families. Originally thought to have an obligate growth requirement for methane and related one-carbon compounds as a source of carbon and energy, it was later shown that various alphaproteobacterial methanotrophs are facultative, able to grow on multi-carbon compounds such as acetate. Most recently, we expanded our knowledge of the metabolic versatility of alphaproteobacterial methanotrophs. We showed that Methylocystis sp. strain SC2 has the capacity for mixotrophic growth on H2 and CH4. This mini-review will summarize the change in perception from the long-held paradigm of obligate methanotrophy to today’s recognition of alphaproteobacterial methanotrophs as having both facultative and mixotrophic capabilities.


2016 ◽  
Vol 20 (3) ◽  
pp. 1 ◽  
Author(s):  
Teng Li ◽  
Caifang Wu

With a burial depth of 1000 m as the demarcation, the coal reservoir in South Yanchuan Block, China is divided into deep reservoir and shallow reservoir regions. A combination of coalbed methane well production data, well logging interpretation, coalbed methane numerical simulations and reservoir properties were used to research various production characteristics at different depths. The results indicate that coal thickness and gas content are not key factors that influence methane production. The shallow reservoir is located in a tension zone, while the deep reservoir is located in both a transformation zone and a compression zone. Although the reservoir and closure pressures increase with the burial depth, the pressures fluctuate in the deep reservoir, especially in the transformation zone. This fluctuation influences the opened degree of the fractures in the reservoir. The effective stress is lower in the deep reservoir than in the shallow reservoir, leading to higher permeability in the deep reservoir. This difference in effective stress is the key factor that influences the methane production. The combination of coal thickness and gas content also significantly influenced the methane production. Influenced by the reservoir and closure pressures, the Type III coal in the shallow reservoir is more developed, while the deep reservoir contained more developed Type I and Type II coal. The permeability increases exponentially with increasing thickness of Type I and Type II coal, which determines the high reservoir permeability in the deep reservoir. The development of Type III coal leads to the poor reservoir hydraulic fracturing effect. However, a reservoir with thick Type I and Type II coal can have a positive effect. Influencia de la presión, la estructura del carbón y su permeabilidad sobre la productividad de gas metano de carbón en profundidades de enterramiento del bloque Yanchuan Sur, ChinaResumenCon una profundidad de enterramiento de 1000 metros, el yacimiento de carbón del bloque Yanchuan Sur, en China, se divide en dos: el depósito profundo y el depósito superficial. Este trabajo combina los datos de la información de producción de gas metano asociado carbón, la interpretación de registros de pozo, las simulaciones numéricas de metano asociado a carbón y las propiedades del reservorio para encontrar las características de producción a diferentes profundidades. Los resultados indican que el espesor del carbón y el contenido de gas no son factores que alcancen a influir en la producción de metano. El depósito superficial se encuentra en una zona de tensión, mientras el depósito profundo está ubicado en una región tanto de transformación como de compresión. Aunque el reservorio y la presión de cierre se incrementan con la profundidad de enterramiento, las presiones fluctúan en el depósito profundo, especialmente en la zona de transformación. Esta fluctuación influye en el grado de apertura de las fracturas en el depósito. La tensión efectiva es más baja en el depósito profundo, lo que significa una mayor permeabilidad. La diferencia en la tensión efectiva es el factor clave que incide en la producción de metano. Afectado por las presiones de cierre y del yacimiento, el carbón tipo III en el depósito superficial está más desarrollado, mientras que el depósito profundo contiene carbón tipo I y tipo II más desarrollado. La permeabilidad se incrementa exponencialmente con el incremento del espesor en el carbón tipo I y tipo II, lo que determina la alta porosidad en el depósito profundo. El desarrollo de carbón tipo III lleva a un pobre efecto de la fractura hidráulica en el depósito. Sin embargo, un depósito con carbón tipo I y tipo II espeso podría tener un efecto positivo.


2019 ◽  
Vol 45 (2) ◽  
pp. 217-228
Author(s):  
Masuma Chowdhury ◽  
Sarmin Sultana ◽  
Md Atikul Islam ◽  
Aziz Hasan ◽  
Kazi Matin Uddin Ahmed

This paper compares the hydrogeochemical signatures between naturally flowing wells with methane (FWs) and non-flowing wells (require pumping) without methane (NFWs) by analyzing a total of 59 groundwater samples to find possible links for methane gas generation. Relatively higher concentration of HCO3 ⁻ indicates oxidation of organic matter (OM) for both the cases which is corroborated by the dominance of Na-Mg-HCO3 and Na- Mg-HCO3-Cl water type. Low concentrations of redox sensitive parameters i.e., NO3 ⁻, SO4 2⁻, Fe2+ and Mn2+ are indicating prevalence of extremely reducing condition and relatively lower concentrations of SO₄²⁻ in FWs suggest microbial sulphate reduction reaching final stage of redox ladder i.e., methanogenesis. Comparatively higher temperature and subsequent higher electrical conductivity (EC) in FWs possibly attributed by the chemical reactions of methane generation or vice versa. Low geothermal gradient, presence of OM and distribution of the FWs over a paleo valley in the deep confined aquifer zone refer to the existence of favourable environment for biogenic methane production rather than the upward migration or leakage of thermogenic gas from the reservoir below. Asiat. Soc. Bangladesh, Sci. 45(2): 217-228, December 2019


1996 ◽  
Vol 30 (8) ◽  
pp. 1885-1893 ◽  
Author(s):  
J.P. Arcangeli ◽  
E. Arvin ◽  
M. Mejlhede ◽  
F.R. Lauritsen

Energies ◽  
2018 ◽  
Vol 11 (10) ◽  
pp. 2577 ◽  
Author(s):  
Dong-Mei Piao ◽  
Young-Chae Song ◽  
Dong-Hoon Kim

This study demonstrated the enhancement of biogenic coal conversion to methane in a bioelectrochemical anaerobic reactor with polarized electrodes. The electrode with 1.0 V polarization increased the methane yield of coal to 52.5 mL/g lignite, which is the highest value reported to the best of our knowledge. The electrode with 2.0 V polarization shortened the adaptation time for methane production from coal, although the methane yield was slightly less than that of the 1.0 V electrode. After the methane production from coal in the bioelectrochemical reactor, the hydrolysis product, soluble organic residue, was still above 3600 mg chemical oxygen demand (COD)/L. The hydrolysis product has a substrate inhibition effect and inhibited further conversion of coal to methane. The dilution of the hydrolysis product mitigates the substrate inhibition to methane production, and a 5.7-fold dilution inhibited the methane conversion rate by 50%. An additional methane yield of 55.3 mL/g lignite was obtained when the hydrolysis product was diluted 10-fold in the anaerobic toxicity test. The biogenic conversion of coal to methane was significantly improved by the polarization of the electrode in the bioelectrochemical anaerobic reactor, and the dilution of the hydrolysis product further improved the methane yield.


1991 ◽  
Vol 260 (6) ◽  
pp. L562-L570 ◽  
Author(s):  
S. H. Randell ◽  
R. Silbajoris ◽  
S. L. Young

During the last stages of intrauterine growth, remarkable changes occur in the alveolar epithelium that include cellular differentiation and increased production of surfactant lipid and apoprotein. We made morphometric measurements of type II cell characteristics from rats aged gestational day 20 to 14 days postnatal. We also measured the amounts of disaturated phosphatidylcholine (DSPC) and surfactant apoprotein (SP-A) in lung tissue, bronchoalveolar lavage, and a lamellar body-rich fraction, and we estimated the lung content of mRNAs for SP-A, SP-B, and SP-C. Lavage and lamellar body surfactant lipid and apoprotein content per lung showed a pattern of a sharp rise in the early postnatal period, then a substantial decline, and a second increase by day 14. When normalized for dry lung weight, the highest DSPC values were found on postnatal day 1 in all compartments. The fraction of whole lung DSPC found in lamellar body or lavage was greatest in the 48-h period surrounding birth. Lamellar body SP-A was greater than lavage SP-A on gestational day 22, but a day later the lavage SP-A was 16 times greater than the lamellar body SP-A. The lung tissue content of all three apoprotein mRNAs increased sharply before birth, fell during the 1st postnatal wk, and then rose again to adult levels. Type II cell number and lamellar body number per milligram of dry lung tissue was highest on post-natal day 1 and fell by one-half during the 1st postnatal wk. The amount of DSPC per unit of lamellar body volume rose to its greatest value on postnatal day 1 and then decreased more than threefold. These findings indicate a pattern of expansion of surfactant cellular and biochemical pools at the time of birth in the rat.


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