Convection behind the Humidification of Titan’s Stratosphere

On Titan, methane is responsible for the complex prebiotic chemistry, the global haze, most of the cloud cover, and the rainfall that models the landscape. Its sources are located in liquid reservoirs at and below the surface, and its sink is the photodissociation at high altitude. Titan’s present and past climates strongly depend on the connection between the surface sources and the atmosphere upper layers. Despite its importance, very little information is available on this topic. In this work, we reanalyze two solar occultations made by Cassini before the northern spring equinox. We find a layer rich in methane at 165 km and at 70°S (mixing ratio 1.62% ± 0.1%) and a dryer background stratosphere (1.1%–1.2%). In the absence of local production, this reveals an intrusion of methane transported into the stratosphere by convective circulation. On the other hand, methane transport through the tropopause at a global scale appears quite inhibited. Leaking through the tropopause is an important bottleneck of Titan’s methane cycle at all timescales. As such, it affects the long-term evolution of Titan’s atmosphere and the exchange fluxes with the surface and subsurface reservoirs in a complex way. Global climate models accounting for cloud physics, thermodynamical feedbacks, and convection are needed to understand the methane cycle, and specifically the humidification of the stratosphere, at the present time, and its evolution under changing conditions at a geological timescale.

Atmospheric methane and nitrous oxide: challenges alongthe path to Net Zero

The causes of methane's renewed rise since 2007, accelerated growth from 2014 and record rise in 2020, concurrent with an isotopic shift to values more depleted in 13 C, remain poorly understood. This rise is the dominant departure from greenhouse gas scenarios that limit global heating to less than 2°C. Thus a comprehensive understanding of methane sources and sinks, their trends and inter-annual variations are becoming more urgent. Efforts to quantify both sources and sinks and understand latitudinal and seasonal variations will improve our understanding of the methane cycle and its anthropogenic component. Nationally declared emissions inventories under the UN Framework Convention on Climate Change (UNFCCC) and promised contributions to emissions reductions under the UNFCCC Paris Agreement need to be verified independently by top-down observation. Furthermore, indirect effects on natural emissions, such as changes in aquatic ecosystems, also need to be quantified. Nitrous oxide is even more poorly understood. Despite this, options for mitigating methane and nitrous oxide emissions are improving rapidly, both in cutting emissions from gas, oil and coal extraction and use, and also from agricultural and waste sources. Reductions in methane and nitrous oxide emission are arguably among the most attractive immediate options for climate action. This article is part of a discussion meeting issue 'Rising methane: is warming feeding warming? (part 1)'.

Adapting macroecology to microbiology: using occupancy modelling to assess functional profiles across metagenomes

Metagenomic sequencing provides information on the metabolic capacities and taxonomic affiliations for members of a microbial community. When assessing metabolic functions in a community, missing genes in pathways can occur in two ways: the genes may legitimately be missing from the community whose DNA was sequenced, or the genes were missed during shotgun sequencing or failed to assemble, and thus the metabolic capacity of interest is wrongly absent from the sequence data. Here, we borrow and adapt occupancy modelling from macroecology to provide mathematical context to metabolic predictions from metagenomes. We review the five assumptions underlying occupancy modelling through the lens of microbial community sequence data. Using the methane cycle, we apply occupancy modelling to examine the presence and absence of methanogenesis and methanotrophy genes from nearly 10,000 metagenomes spanning global environments. We determine that methanogenesis and methanotrophy are positively correlated across environments, and note that the lack of available standardized metadata for most metagenomes is a significant hindrance to large-scale statistical analyses. We present this adaptation of macroecology’s occupancy modelling to metagenomics as a tool for assessing presence/absence of traits in environmental microbiological surveys. We further initiate a call for stronger metadata standards to accompany metagenome deposition, to enable robust statistical approaches in the future.

Methane flux and gas hydrate accumulations in the Sea of Okhotsk and their ecological aspects

Methane is one of the important representatives of the organic substances in the atmosphere (for example, an increase of methane content in the atmosphere can affect enhancing the greenhouse effect). Gas hydrates are an essential part of links in the methane cycle and the accompanying fluxes of other gases. The research object in this paper is the Sea of Okhotsk, where gas hydrate fields and active submarine gas discharge areas were found. The study of methane fluxes is relevant both for the Sea of Okhotsk and the World Ocean. From 1984 to 2015, the background and anomalous methane fields were explored in the Sea of Okhotsk as a result of gas geochemical research carried out by scientists of the Gasgeochemistry Laboratory (POI FEB RAS). The flux of natural gas became stronger from the sources to the seafloor, from the seafloor to the water, and, finally, to the atmosphere due to renew of fault zones. Moreover, the amount of methane vents on the Sakhalin slope has increased from 2-3 vents to more than 400. The most representative hydroacoustic anomalies “flares” mapped direct methods study methane hydrates. The received outcomes indicate the urgency of the study of methane fluxes and the formation-dissociation mechanism of gas hydrates, and the influence of natural gas from hydrocarbon sources on the environment.

Maintaining grass coverage increases methane uptake in Amazonian pasture soils

Cattle ranching is the largest driver of deforestation in the Brazilian Amazon. The rainforest-to-pasture conversion affects the methane cycle in upland soils, changing it from sink to source of atmospheric methane. However, it remains unknown if management practices could reduce the impact of land-use on methane cycling. In this work, we evaluated how pasture management can regulate the soil methane cycle either by maintaining continuous grass coverage on pasture soils, or by liming the soil to amend acidity. Methane fluxes from forest and pasture soils were evaluated in moisture-controlled greenhouse experiments with and without grass cover (Urochloa brizantha cv. Marandu) or liming. In parallel, we assessed changes in the soil microbial community structure of both bare pasture soil as well as rhizosphere soil through high throughput sequencing of the 16S rRNA gene, and quantified the methane cycling microbiota by their respective marker genes related to methane generation (mcrA) or oxidation (pmoA). The experiments used soils from eastern and western Amazonia, and concurrent field studies allowed us to confirm greenhouse data. The presence of a grass cover not only increased methane uptake by up to 35% in pasture soils, but also reduced the abundance of the methane-producing community. In the grass rhizosphere this reduction was up to 10-fold. Methane-producing archaea belonged to the genera Methanosarcina sp., Methanocella sp., Methanobacterium sp., and Rice Cluster I. Further, we showed that liming compromised the capacity of forest and pasture soils to be a sink for methane, and instead converted formerly methane-consuming forest soils to become methane sources in only 40-80 days. Our results demonstrate that pasture management that maintains grass coverage can mitigate soil methane emissions, if compared to a bare pasture soil.

Methane gas emissions from savanna fires: What analysis of local burning regimes in a working West African landscape tell us

Savanna fires contribute significantly to greenhouse gas emissions. While it is recognized that these fires play an important role in the global methane cycle, there are too few accurate estimates of emissions from West Africa, the continent's most active fire region. Most estimates of methane emissions contain high levels of uncertainty because they are based on generalizations of diverse landscapes that are burned by complex fire regimes. To improve estimates we used an approach grounded in the burning practices of people who set fires to working landscapes. We conducted 97 experimental fires collecting data for savanna type, grass type, biomass composition and amount consumed, scorch height, speed of fire front, fire type and ambient air conditions for two sites in Mali. We collected smoke samples for 36 fires using a canister method. We report values for fire intensity, combustion completeness, patchiness, modified combustion efficiency (MCE) and emission factor (EF). Our study finds that methane EFs ranged from 3.71 g/kg in the early dry season (EDS) to 2.86 in the mid-dry season (MDS). We found head fires had nearly double the CH4 EF of backfires (4.89 g/kg to 2.92). Fires during the MDS have the lowest intensity values and the lowest methane emissions 0.981 g/m2 compared with 1.030 g/m2 for EDS and 1.102 g/m2 for the late dry season (LDS). We conclude that policies aimed at shifting the burning regime earlier to reduce methane emissions will not have the desired effects, especially if fire type is not considered. We recommend using the adjusted mean value of 0.862 g/m2—based on the carbon content for West African grasses—for calculating emissions for West African savannas.

Ancient warming pushed wetlands across biogeochemical tipping points

<p>Ancient peat deposits provide valuable and complementary insight into the biogeochemical response of wetlands to climate perturbations, including potential tipping points in such systems. The combination of temperature (GDGTs) and hydrology (leaf wax hydrogen isotopic compositions) proxies with qualitative proxies for methanogenesis (archaeal lipid abundances) and methanotrophy (bacterial lipid carbon isotopic compositions) has revealed dramatic perturbations to the carbon cycle during transient warming events, including the Palaeocene Eocene Thermal Maximum.  Bacterially-derived hopanes in at least two PETM-spanning lignite sequences record negative carbon isotope excursions of near-unprecedented magnitude in response to rapid global warming.  The warming – either directly or indirectly – clearly caused a fundamental reorganisation of the carbon cycle in those ancient wetlands. Intriguingly however, these excursions persist for a far shorter duration than the PETM warming. Similarly, hopane δ<sup>13</sup>C values in lignites of the Early Eocene Climate Optimum, the warmth of which was reached more gradually, are similar to those of today. This suggests that these unusually isoptopically light hopanoids represent a transient disruption to the methane cycle associated with a climate perturbation rather than an equilibrium response to warmer temperatures.  Such an interpretation is consistent with Deglacial and Holocene peat-derived records, in which hopane δ<sup>13</sup>C values exhibit large responses to transient drying events and modest responses to longer-term change. Such findings could have implications for future climate change feedbacks, with the wetland methane cycle being particularly sensitive to the rate of climatic change.</p>

Microbial Communities in Methane Cycle: Modern Molecular Methods Gain Insights into Their Global Ecology

The role of methane as a greenhouse gas in the concept of global climate changes is well known. Methanogens and methanotrophs are two microbial groups which contribute to the biogeochemical methane cycle in soil, so that the total emission of CH4 is the balance between its production and oxidation by microbial communities. Traditional identification techniques, such as selective enrichment and pure-culture isolation, have been used for a long time to study diversity of methanogens and methanotrophs. However, these techniques are characterized by significant limitations, since only a relatively small fraction of the microbial community could be cultured. Modern molecular methods for quantitative analysis of the microbial community such as real-time PCR (Polymerase chain reaction), DNA fingerprints and methods based on high-throughput sequencing together with different “omics” techniques overcome the limitations imposed by culture-dependent approaches and provide new insights into the diversity and ecology of microbial communities in the methane cycle. Here, we review available knowledge concerning the abundances, composition, and activity of methanogenic and methanotrophic communities in a wide range of natural and anthropogenic environments. We suggest that incorporation of microbial data could fill the existing microbiological gaps in methane flux modeling, and significantly increase the predictive power of models for different environments.

Anthropogenic and Environmental Constraints on the Microbial Methane Cycle in Coastal Sediments

Large amounts of methane, a potent greenhouse gas, are produced in anoxic sediments by methanogenic archaea. Nonetheless, over 90% of the produced methane is oxidized via sulfate-dependent anaerobic oxidation of methane (S-AOM) in the sulfate-methane transition zone (SMTZ) by consortia of anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing bacteria (SRB). Coastal systems account for the majority of total marine methane emissions and typically have lower sulfate concentrations, hence S-AOM is less significant. However, alternative electron acceptors such as metal oxides or nitrate could be used for AOM instead of sulfate. The availability of electron acceptors is determined by the redox zonation in the sediment, which may vary due to changes in oxygen availability and the type and rate of organic matter inputs. Additionally, eutrophication and climate change can affect the microbiome, biogeochemical zonation, and methane cycling in coastal sediments. This review summarizes the current knowledge on the processes and microorganisms involved in methane cycling in coastal sediments and the factors influencing methane emissions from these systems. In eutrophic coastal areas, organic matter inputs are a key driver of bottom water hypoxia. Global warming can reduce the solubility of oxygen in surface waters, enhancing water column stratification, increasing primary production, and favoring methanogenesis. ANME are notoriously slow growers and may not be able to effectively oxidize methane upon rapid sedimentation and shoaling of the SMTZ. In such settings, ANME-2d (Methanoperedenaceae) and ANME-2a may couple iron- and/or manganese reduction to AOM, while ANME-2d and NC10 bacteria (Methylomirabilota) could couple AOM to nitrate or nitrite reduction. Ultimately, methane may be oxidized by aerobic methanotrophs in the upper millimeters of the sediment or in the water column. The role of these processes in mitigating methane emissions from eutrophic coastal sediments, including the exact pathways and microorganisms involved, are still underexplored, and factors controlling these processes are unclear. Further studies are needed in order to understand the factors driving methane-cycling pathways and to identify the responsible microorganisms. Integration of the knowledge on microbial pathways and geochemical processes is expected to lead to more accurate predictions of methane emissions from coastal zones in the future.

Carbon Isotope Chemostratigraphy Across the Permian-Triassic Boundary at Chaotian, China: Implications for the Global Methane Cycle in the Aftermath of the Extinction

During the end-Permian extinction, a substantial amount of methane (CH4) was likely released into the ocean-atmosphere system associated with the Siberian Traps volcanism, although fluctuations in the global CH4 cycle in the aftermath of the extinction remain poorly understood. The carbon (C) isotopic composition of carbonate (δ13Ccarb) across the Permian-Triassic boundary (P-TB) was analyzed at Chaotian, South China. The δ13Ccarb values decrease from ca. +1 to –2‰ across the P-TB, possibly caused by a collapse of primary productivity associated with the shallow-marine extinction. The frequent intercalation of felsic tuff layers around the P-TB suggests that a volcanogenic carbon dioxide (CO2) input to the surface oceans may also have contributed to the δ13Ccarb decline. The magnitude of the δ13Ccarb decrease (∼3‰) is substantially smaller than the magnitude of a decrease in C isotopic composition of organic matter (δ13Corg) in the same P-TB interval (∼7‰). This apparent δ13Ccarb-δ13Corg decoupling could be explained by proliferation of methanogen (“methanogenic burst”) in the sediments. A global δ13C compilation shows a large variation in marine δ13Corg records, implying that the “methanogenic burst” according to the Siberian Traps volcanism may have contributed, at least in part, to the δ13Corg variability and to the elevated CH4 levels in the atmosphere. The present and previous observations allow us to infer that the global CH4 cycle may have fluctuated substantially in the aftermath of the extinction.