Laser Microsurgery Permits Fungal Plasma Membrane Single-Ion-Channel Resolution at the Hyphal Tip
1998 ◽
Vol 64
(4)
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pp. 1569-1572
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Keyword(s):
ABSTRACT A method for formation of high-electrical-resistance seals on theNeurospora crassa plasma membrane, allowing resolution of single-ion-channel activity by patch clamp electrophysiology, is reported. Laser microsurgery permits access to the hyphal apex without enzymatic cell wall digestion and loss of morphological polarity. Cell wall reformation is delayed by brefeldin. This method can allow full characterization of apical plasma membrane channels, which are implicated in tip growth.
1987 ◽
Vol 96
(2)
◽
pp. 175-186
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1987 ◽
Vol 34
(3)
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pp. 407-413
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Keyword(s):
1997 ◽
Vol 137
(4)
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pp. 579-585
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1971 ◽
Vol 29
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pp. 262-263
1993 ◽
Vol 3
(5)
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pp. 637-646
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1990 ◽
Vol 80
(4)
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pp. 629-635
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