A Flow Cytometric Method for Rapid Selection of Novel Industrial Yeast Hybrids

1998 ◽  
Vol 64 (5) ◽  
pp. 1669-1672 ◽  
Author(s):  
P. J. L. Bell ◽  
D. Deere ◽  
J. Shen ◽  
B. Chapman ◽  
P. H. Bissinger ◽  
...  
Keyword(s):  
Cell Sorting ◽  
Parent Strain ◽  
Industrial Yeast ◽  
Green Fluorescence ◽  
Color Flow ◽  
Flow Cytometric Method ◽  
Flow Cytometric ◽  
Yeast Strains ◽  
Flow Cytometric Cell ◽  
Selection Of

ABSTRACT We rapidly produced and isolated novel yeast hybrids by using two-color flow cytometric cell sorting. We labeled one parent strain with a fluorescent green stain and the other parent with a fluorescent orange stain, and hybrids were selected based on their dual orange and green fluorescence. When this technique was applied to the production of hybrids by traditional mating procedures, more than 96% of the isolates were hybrids. When it was applied to rare mating, three hybrids were identified among 50 isolates enriched from a population containing 2 � 106 cells. This technology is not dependent on genetic markers and has applications in the development of improved industrial yeast strains.

Selection of defined cell types by flow-cytometric cell sorting

1993 ◽  
Vol 11 (2) ◽  
pp. 55-62 ◽  
Author(s):  
Hans J. Tanke ◽  
Maarten van der Keur
Keyword(s):  
Cell Sorting ◽  
Cell Types ◽  
Flow Cytometric ◽  
Flow Cytometric Cell ◽  
Selection Of

scholarly journals A 5-color flow cytometric method for extended 8-part leukocyte differential

2017 ◽  
Vol 92 (6) ◽  
pp. 498-507 ◽  
Author(s):  
Julien Guy ◽  
Orianne Wagner-Ballon ◽  
Olivier Pages ◽  
François Bailly ◽  
Jessica Borgeot ◽  
...  
Keyword(s):  
Color Flow ◽  
Flow Cytometric Method ◽  
Flow Cytometric

scholarly journals Fluorescence-Activated Cell Sorting of EGFP-Labeled Neural Crest Cells From Murine Embryonic Craniofacial Tissue

2005 ◽  
Vol 2005 (3) ◽  
pp. 232-237 ◽  
Author(s):  
Saurabh Singh ◽  
Vasker Bhattacherjee ◽  
Partha Mukhopadhyay ◽  
Christopher A. Worth ◽  
Samuel R. Wellhausen ◽  
...  
Keyword(s):  
Neural Crest ◽  
Cell Sorting ◽  
Present Report ◽  
Neural Crest Cells ◽  
Egfp Expression ◽  
Marker Genes ◽  
Flow Cytometric Method ◽  
Flow Cytometric ◽  
Polymerase Chain ◽  
Craniofacial Tissue

During the early stages of embryogenesis, pluripotent neural crest cells (NCC) are known to migrate from the neural folds to populate multiple target sites in the embryo where they differentiate into various derivatives, including cartilage, bone, connective tissue, melanocytes, glia, and neurons of the peripheral nervous system. The ability to obtain pure NCC populations is essential to enable molecular analyses of neural crest induction, migration, and/or differentiation. CrossingWnt1-CreandZ/EGtransgenic mouse lines resulted in offspring in which theWnt1-Cretransgene activated permanent EGFP expression only in NCC. The present report demonstrates a flow cytometric method to sort and isolate populations of EGFP-labeled NCC. The identity of the sorted neural crest cells was confirmed by assaying expression of known marker genes by TaqMan Quantitative Real-Time Polymerase Chain Reaction (QRT-PCR). The molecular strategy described in this report provides a means to extract intact RNA from a pure population of NCC thus enabling analysis of gene expression in a defined population of embryonic precursor cells critical to development.

scholarly journals Two-color flow cytometric measurement of DNA distributions of rat megakaryocytes in unfixed, unfractionated marrow cell suspensions

Blood ◽  
1984 ◽  
Vol 63 (4) ◽  
pp. 768-778 ◽  
Author(s):  
CW Jackson ◽  
LK Brown ◽  
BC Somerville ◽  
SA Lyles ◽  
AT Look
Keyword(s):  
Rapid Determination ◽  
Marrow Cell ◽  
Green Fluorescence ◽  
Color Flow ◽  
Red Fluorescence ◽  
Flow Cytometric ◽  
Clinical Investigations ◽  
Flow Cytometric Measurement ◽  
Fcm Analysis ◽  
Cellular Dna

Abstract The ploidy distribution of megakaryocytes shifts in response to platelet demand and thus provides a sensitive index of megakaryocytopoiesis. Flow cytometry (FCM) is a potentially valuable method for rapid determination of ploidy distributions of megakaryocyte populations; however, because megakaryocytes constitute only a very small proportion of the cells in unfractionated marrow, other rare events, such as cell clumping, complicate FCM analysis. We describe the measurement of cellular DNA distributions of megakaryocytes by two- color FCM in unfixed, unfractionated marrow--a method based on the resistance of megakaryocytes to hypotonic lysis in the cold for at least 2 days. Specific platelet antiserum was used to label megakaryocytes by indirect immunofluorescence with fluorescein (green fluorescence), and DNA was stained with propidium iodide (red fluorescence) in hypotonic citrate solution. The ploidy distribution of megakaryocytes was selectively determined with two-color, green-gated FCM, with which the red and green fluorescence of all cells is analyzed, but only the red fluorescence (DNA content) of cells that specifically bound the platelet antibody is recorded. We demonstrate that this method can readily detect changes in megakaryocyte DNA distributions due to experimental thrombocytopenia or platelet hypertransfusion and, therefore, should be useful for both experimental and clinical investigations of megakaryocytopoiesis.

scholarly journals Reimagining Flow Cytometric Cell Sorting

2020 ◽  
Vol 4 (8) ◽  
pp. 2000019
Author(s):  
Suwan N. Jayasinghe
Keyword(s):  
Cell Sorting ◽  
Flow Cytometric ◽  
Flow Cytometric Cell

Isolation of Mouse Pancreatic Ductal Progenitor Cells Expressing CD133 and c-Met by Flow Cytometric Cell Sorting

2007 ◽  
Vol 132 (2) ◽  
pp. 720-732 ◽  
Author(s):  
Yuji Oshima ◽  
Atsushi Suzuki ◽  
Kaneaki Kawashimo ◽  
Momotarou Ishikawa ◽  
Nobuhiro Ohkohchi ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Cell Sorting ◽  
Flow Cytometric ◽  
Flow Cytometric Cell
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