scholarly journals Identification and Characterization of Leuconostoc fallax Strains Isolated from an Industrial Sauerkraut Fermentation

2002 ◽  
Vol 68 (6) ◽  
pp. 2877-2884 ◽  
Author(s):  
Rodolphe Barrangou ◽  
Sung-Sik Yoon ◽  
Frederick Breidt, ◽  
Henry P. Fleming ◽  
Todd R. Klaenhammer
Keyword(s):  
Lactic Acid ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Variable Regions ◽  
Random Amplification ◽  
End Products ◽  
Identification And Characterization ◽  
The 16S Rrna Gene ◽  
Endonuclease Digestion

ABSTRACT Lactic acid bacterial strains were isolated from brines sampled after 7 days of an industrial sauerkraut fermentation, and six strains were selected on the basis of susceptibility to bacteriophages. Bacterial growth in cabbage juice was monitored, and the fermentation end products were identified, quantified, and compared to those of Leuconostoc mesenteroides. Identification by biochemical fingerprinting, endonuclease digestion of the 16S-23S intergenic transcribed spacer region, and sequencing of variable regions V1 and V2 of the 16S rRNA gene indicated that the six selected sauerkraut isolates were Leuconostoc fallax strains. Random amplification of polymorphic DNA fingerprints indicated that the strains were distinct from one another. The growth and fermentation patterns of the L. fallax isolates were highly similar to those of L. mesenteroides. The final pH of cabbage juice fermentation was 3.6, and the main fermentation end products were lactic acid, acetic acid, and mannitol for both species. However, none of the L. fallax strains exhibited the malolactic reaction, which is characteristic of most L. mesenteroides strains. These results indicated that in addition to L. mesenteroides, a variety of L. fallax strains may be present in the heterofermentative stage of sauerkraut fermentation. The microbial ecology of sauerkraut fermentation appears to be more complex than previously indicated, and the prevalence and roles of L. fallax require further investigation.

Ruegeria pelagia sp. nov., isolated from the Sargasso Sea, Atlantic Ocean

2007 ◽  
Vol 57 (8) ◽  
pp. 1815-1818 ◽  
Author(s):  
Kiyoung Lee ◽  
Yoe-Jin Choo ◽  
Stephen J. Giovannoni ◽  
Jang-Cheon Cho
Keyword(s):  
Phylogenetic Analyses ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
Sargasso Sea ◽  
Bacterial Strains ◽  
Ruegeria Pomeroyi ◽  
Distinct Line ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

Gram-negative, facultatively aerobic, chemoheterotrophic, short rod-shaped marine bacterial strains HTCC2662T and HTCC2663, isolated from the Sargasso Sea by using a dilution-to-extinction culturing method, were investigated to determine their taxonomic position. Characterization of the two strains by phenotypic and phylogenetic analyses revealed that they belonged to the same species. The DNA G+C content of strain HTCC2662T was 58.4 mol% and the predominant cellular fatty acids were C18 : 1 ω7c (52.5 %), C16 : 0 2-OH (13.5 %) and C18 : 1 11-methyl ω7c (12.2 %). Phylogenetic analysis of the 16S rRNA gene sequences showed that the strains represented a distinct line of descent within the genus Ruegeria, with highest sequence similarities to Ruegeria atlantica DSM 5823T (97.2 %), Ruegeria lacuscaerulensis DSM 11314T (96.5 %) and Ruegeria pomeroyi DSM 15171T (95.6 %). Several phenotypic characteristics, including facultatively requiring NaCl and oxygen for growth, together with the cellular fatty acid composition, differentiated strain HTCC2662T from other members of the genus Ruegeria. Based on phenotypic, chemotaxonomic and phylogenetic traits, it is suggested that strains HTCC2662T and HTCC2663 represent a novel species of the genus Ruegeria, for which the name Ruegeria pelagia sp. nov. is proposed. The type strain is HTCC2662T (=KCCM 42378T=NBRC 102038T).

Identification and Characterization of Enterococcus spp. in Greek Spontaneous Sausage Fermentation

2008 ◽  
Vol 71 (6) ◽  
pp. 1244-1247 ◽  
Author(s):  
SPIROS PARAMITHIOTIS ◽  
DAFNI-MARIA KAGKLI ◽  
VASILIKI A. BLANA ◽  
GEORGE-JOHN E. NYCHAS ◽  
ELEFTHERIOS H. DROSINOS
Keyword(s):  
Phenotypic Traits ◽  
Rrna Gene ◽  
Strain Typing ◽  
Phenotypic Properties ◽  
Fermented Sausages ◽  
Enterococcus Spp ◽  
Identification And Characterization ◽  
The 16S Rrna Gene

A total of 108 enterococcal strains previously isolated from spontaneously fermented sausages were identified using phenotypic traits, pulsed-field gel electrophoresis (PFGE), and sequencing of the 16S rRNA gene. The proteolytic and lipolytic activities of these isolates and their ability to decarboxylate lysine, tyrosine, ornithine, and histidine and to produce antimicrobial compounds also were assessed. All strains were identified as Enterococcus faecium, and a lack of correlation between data derived from phenotypic and those derived from genotypic techniques was evident. Wide strain diversity was revealed by both phenotypic properties and PFGE strain typing results. Few strains were present in all batches, suggesting a possible persistence in the respective production plants. Neither proteolytic nor lipolytic activities were detected, and none of the strains decarboxylated lysine, tyrosine, ornithine, or histidine. A total of 42 E. faecium strains inhibited in vitro growth of Listeria monocytogenes, which suggests possible contribution of these strains to the safety of the end product and possible utilization of these strains as protective cultures.

scholarly journals Integral Characterization of the 16S rRNA Gene of Non-sporulating Bacteria and Its Action Against Anticarsia gemmatalis Hübner (Lepidoptera: Erebidae)

2020 ◽  
Vol 12 (12) ◽  
pp. 61
Author(s):  
Higor de Oliveira Alves ◽  
Mariana Davanzo Miranda ◽  
Ricardo Antônio Polanczyk ◽  
Joacir do Nascimento ◽  
Janete Apparecida Desiderio ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Marker Gene ◽  
Anticarsia Gemmatalis ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Velvetbean Caterpillar ◽  
The 16S Rrna Gene

Brazil is the world’s largest producer of soybean (Glycine max), an extremely important legume due to its source of proteins and essential oils for humans and animals, besides to its applications in the various branches of industry. The velvetbean caterpillar [Anticarsia gemmatalis Hübner (Lepidoptera: Erebidae)] is a great pest that affects this crop and has been controlled by chemical and biological pesticides based on Bacillus thuringiensis. The objectives of this work were to prospect soil microorganisms, to characterize them using the 16S rRNA gene and to perform bioassays to analyze the lethality or subletality of these isolates against A. gemmatalis larvae. The DNA sequencing of the marker gene was complete, covering all conserved regions of it to determine the phylogenetic position of the isolates. Regarding to bioassays, subletality efficacy were low both for sporulant and for the non-sporulant bacterial strains tested. However, based on the signature by complete 16S rRNA analyses of the non-sporulating bacterial isolates, new characteristics worth of studying and prospecting biotechnologically became available.

scholarly journals Identification and characterization of lactic acid bacteria isolated from traditional pickles in Sichuan, China

2012 ◽  
Vol 58 (3) ◽  
pp. 163-172 ◽  
Author(s):  
Jie Yu ◽  
Wa Gao ◽  
Manjun Qing ◽  
Zhihong Sun ◽  
Weihong Wang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Identification And Characterization

scholarly journals Isolation and Characterization of Lactic Acid Bacteria in Philippine Fermented Milkfish Chanos chanos-Rice Mixture (Burong Bangus)

2018 ◽  
Vol 6 (2) ◽  
pp. 500-508
Author(s):  
Julie Ann A. Arcales ◽  
Garner Algo L.Alolod
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Enzymatic Activity ◽  
Antimicrobial Property ◽  
Food Products ◽  
The Philippines ◽  
Lactobacillus Delbrueckii ◽  
Bacterial Strains ◽  
Isolation And Characterization

Isolation and characterization of bacteria in food products are important to determine and distinguish the beneficial or harmful effects of microbiota in certain samples. Lactic acid bacteria in food products had long been associated to good factors as food preservatives and with added fermentation metabolites. This study isolated and characterized lactic acid bacteria from burong bangus. The culture and purification process of bacteria isolation resulted to 4 strains of lactic acid bacteria namely Enterococcus faecalis, Tetragenococcus muriaticus, Lactobacillus delbrueckii subp. delbrueckii and Carnobacterium divergens. High enzymatic activity were observed with E. faecalis particularly on lipase and protease assay. While C. divergens have no enzymatic activity against lipase, protease, amylase and cellulase. The antimicrobial property of L. delbrueckii is only susceptible to amoxicillin unlike the other three bacteria isolates. No antagonistic activity were observed with the four bacterial strains against Bacillus subtilis, Staphylococcus aureus and Escherichia coli. The result of this study showed promising benefits to the industry especially in developing countries like the Philippines because population are not yet so aware of this organisms and the benefits that can be derived through their consumption.

scholarly journals Molecular characterization of Streptococcus mutans gtfB gene isolated from families

2018 ◽  
Vol 33 (1) ◽  
pp. 40
Author(s):  
Monica Moreira ◽  
Mariana Machado Fidelis do Nascimento ◽  
Mariane Moreira Poletto ◽  
Débora Do Rocio Kliisiowicz ◽  
Renata Rodrigues Gomes ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Dental Enamel ◽  
Extracellular Polysaccharides ◽  
Rrna Gene ◽  
Partial Sequencing ◽  
Dental Biofilm ◽  
Potential Marker ◽  
Random Amplification ◽  
Genetic Pattern

OBJECTIVE: Caries is a multifactorial infectious disease and the main etiological agent is the bacteria Streptococcus mutans due to its virulence factors, which enable the adherence to dental enamel and favours the formation of dental biofilm through the production of extracellular polysaccharides. The transmission of S. mutans can occur between people, often within families. The present study aimed to evaluate the intrafamily genetic pattern of S. mutans through partial sequencing of the gene that encodes the glucosyltransferase β (gtfB) enzyme.METHODS: We previously analyzed saliva samples from 40 individuals from nine families, and it was obtained 64 isolates biochemically characterized as S. mutans. The isolates were evaluated by random amplification of polymorphic DNA (RAPD). Those with greater similarity were characterized by partial sequence 16S rRNA gene and the gtfB gene sequencing.RESULTS: It was observed genetic similarity among strains isolated from individuals with caries activity; while isolates from individual without caries showed that they are genetically distinct, suggesting a different virulence pattern.CONCLUSION: The present results demonstrated that partial sequencing of the gtfB gene showed to be a potential marker to investigate genetic pattern and virulence of S. mutans, deserving further investigation in order to identify families at risk of caries.

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