scholarly journals Rhizobium leguminosarum Biovar viciae 1-Aminocyclopropane-1-Carboxylate Deaminase Promotes Nodulation of Pea Plants

2003 ◽  
Vol 69 (8) ◽  
pp. 4396-4402 ◽  
Author(s):  
Wenbo Ma ◽  
Frèdèrique C. Guinel ◽  
Bernard R. Glick
Keyword(s):  
Pisum Sativum ◽  
Rhizobium Leguminosarum ◽  
Higher Plants ◽  
Regulatory Gene ◽  
Regulatory Protein ◽  
Acc Deaminase ◽  
Ethylene Biosynthesis ◽  
Plant Roots ◽  
Nodule Development ◽  
Insertion Mutants

ABSTRACT Ethylene inhibits nodulation in various legumes. In order to investigate strategies employed by Rhizobium to regulate nodulation, the 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene was isolated and characterized from one of the ACC deaminase-producing rhizobia, Rhizobium leguminosarum bv. viciae 128C53K. ACC deaminase degrades ACC, the immediate precursor of ethylene in higher plants. Through the action of this enzyme, ACC deaminase-containing bacteria can reduce ethylene biosynthesis in plants. Insertion mutants with mutations in the rhizobial ACC deaminase gene (acdS) and its regulatory gene, a leucine-responsive regulatory protein-like gene (lrpL), were constructed and tested to determine their abilities to nodulate Pisum sativum L. cv. Sparkle (pea). Both mutants, neither of which synthesized ACC deaminase, showed decreased nodulation efficiency compared to that of the parental strain. Our results suggest that ACC deaminase in R. leguminosarum bv. viciae 128C53K enhances the nodulation of P. sativum L. cv. Sparkle, likely by modulating ethylene levels in the plant roots during the early stages of nodule development. ACC deaminase might be the second described strategy utilized by Rhizobium to promote nodulation by adjusting ethylene levels in legumes.

scholarly journals Expression of an Exogenous 1-Aminocyclopropane-1-Carboxylate Deaminase Gene in Sinorhizobium meliloti Increases Its Ability To Nodulate Alfalfa

2004 ◽  
Vol 70 (10) ◽  
pp. 5891-5897 ◽  
Author(s):  
Wenbo Ma ◽  
Trevor C. Charles ◽  
Bernard R. Glick
Keyword(s):  
Sinorhizobium Meliloti ◽  
Rhizobium Leguminosarum ◽  
Higher Plants ◽  
Regulatory Gene ◽  
Regulatory Protein ◽  
Acc Deaminase ◽  
Plasmid Vector ◽  
Plant Growth Promoting Rhizobacteria ◽  
Infection Threads ◽  
Plant Growth Promoting

ABSTRACT 1-Aminocyclopropane-1-carboxylate (ACC) deaminase has been found in various plant growth-promoting rhizobacteria, including rhizobia. This enzyme degrades ACC, the immediate precursor of ethylene, and thus decreases the biosynthesis of ethylene in higher plants. The ACC deaminase of Rhizobium leguminosarum bv. viciae 128C53K was previously reported to be able to enhance nodulation of peas. The ACC deaminase structural gene (acdS) and its upstream regulatory gene, a leucine-responsive regulatory protein (LRP)-like gene (lrpL), from R. leguminosarum bv. viciae 128C53K were introduced into Sinorhizobium meliloti, which does not produce this enzyme, in two different ways: through a plasmid vector and by in situ transposon replacement. The resulting ACC deaminase-producing S. meliloti strains showed 35 to 40% greater efficiency in nodulating Medicago sativa (alfalfa), likely by reducing ethylene production in the host plants. Furthermore, the ACC deaminase-producing S. meliloti strain was more competitive in nodulation than the wild-type strain. We postulate that the increased competitiveness might be related to utilization of ACC as a nutrient within the infection threads.

scholarly journals SOYBEAN SEEDLING ROOT GROWTH PROMOTION BY 1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-PRODUCING PSEUDOMONADS

2016 ◽  
Vol 10 (1) ◽  
pp. 19 ◽  
Author(s):  
Edi Husen ◽  
Aris Tri Wahyudi ◽  
Antonius Suwanto ◽  
Rasti Saraswati
Keyword(s):  
Root Growth ◽  
Higher Plants ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Ethylene Biosynthesis ◽  
Soil Biology ◽  
Soybean Root ◽  
Soybean Seedlings ◽  
Local Soil ◽  
Growth Room

Pseudomonad producing 1-aminocyclopropane-1-carboxylate (ACC) deaminase (E.C.4.1.99.4) has been known to promote plant growth by lowering ethylene biosynthesis in higher plants, which can be induced by indole-3-acetic acid (IAA) production. The objective of this study was to examine the ability of IAAproducing Pseudomonas isolated from local soil environment (rhizosphere of soybean grown in Plumbon's agricultural area<br />in Cirebon, West Java, Indonesia) to promote soybean root growth in relation to their ACC deaminase activities. The experiments were conducted in growth room and Laboratory of Soil Biology Research, Indonesian Soil Research Institute, Bogor, from January to August 2008. Soybean seeds were inoculated by immersing the seeds for 1 hour in bacterial cell suspension containing approximately 108-109 cells ml-1. The seeds were then germinated<br />for 2 days before planting in growth pouches containing sterilized distilled water. All treated and untreated seeds were grown for 7 days in growth room at 24°C with 1300 lux of light intensity for 12-hour followed by a 12-hour dark period at 22°C. ACC deaminase activity of the isolates was assayed based on their ability to grow in Dworkin-Foster’s salt minimal medium containing ammonium sulfate or ACC as a source of nitrogen. Thirteen out of 81 isolates tested significantly increased soybean root length and weight, up to 50% from untreated plants. Of 13 isolates, 11 demonstrated ACC deaminase activities. Two isolates that did not show ACC deaminase activities had lower capacity to produce IAA. The results suggest that the effectiveness of IAA producing Pseudomonas in promoting the growth of the soybean seedlings is associated with their ACC deaminase activities or they produce IAA at low levels.

scholarly journals The Role of Rhizobial ACC Deaminase in the Nodulation Process of Leguminous Plants

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Francisco X. Nascimento ◽  
Clarisse Brígido ◽  
Bernard R. Glick ◽  
Márcio J. Rossi
Keyword(s):  
Stress Responses ◽  
Higher Plants ◽  
Growth Promotion ◽  
Agricultural Practices ◽  
Acc Deaminase ◽  
Nodule Development ◽  
Nodule Formation ◽  
Direct Role ◽  
Sustainable Agricultural Practices

Symbiotic rhizobia-legumes associations are extremely important in terms of sustainable agricultural practices. This symbiosis involves a complex interaction between both partners, plant and bacterium, for bacterial infection and the formation of symbiotic N-fixing nodules. In this regard, the phytohormone ethylene plays a significant role in nodule formation, acting as an inhibitor of the nodulation process. Ethylene not only regulates nodule development but also regulates many other plant developmental cues, including various stress responses that inhibit overall plant growth. Some rhizobia produce the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, thus, being able to decrease ACC and, consequently, decrease deleterious ethylene levels that affect the nodulation process. This occurs because ACC is the immediate precursor of ethylene in all higher plants. Hence, rhizobia that express this enzyme have an increased symbiotic potential. In addition to the direct role that ACC deaminase plays in the nodulation processper se, in a limited number of instances, ACC deaminase can also modulate nodule persistence. This review focuses on the important role of rhizobial ACC deaminase during the nodulation process, emphasizing its significance to legume growth promotion.

Effects of addition of flavonoid signals and environmental factors on nodulation and nodule development in the pea (Pisum sativum) - Rhizobium leguminosarum bv. viciae symbiosis

Soil Research ◽  
2003 ◽  
Vol 41 (2) ◽  
pp. 267 ◽  
Author(s):  
Mario de A. Lira Junior ◽  
Carlos Costa ◽  
Donald L. Smith
Keyword(s):  
Pisum Sativum ◽  
Rhizobium Leguminosarum ◽  
Nitrogen Availability ◽  
Root Zone ◽  
Nodule Development ◽  
Controlled Environment ◽  
Signal Molecule ◽  
Root Zone Temperature ◽  
Low Root Zone Temperature ◽  
Zone Temperature

The legume–Rhizobium symbiosis is the most important source of biologically fixed nitrogen in agricultural systems. However, it is complex and sensitive to environmental effects, including available mineral nitrogen, soil salinity, and low root-zone temperature. How these factors inhibit the symbiosis is not well understood. If the effects are mostly on the early stages of nodulation, addition of signal molecule(s) may overcome it. Pisum sativum seeds were germinated and the seedlings were inoculated with bacterial culture and cultivated under controlled environment conditions, studying each of the above nodulation-inhibitory factors, under 3 levels: control (little or no inhibitory condition), and moderately or severely inhibitory conditions. Aspects of nodule development (size and number) were measured with a scanner-based technology. All of the environmental conditions studied had effects on both nodule establishment and development. The addition of either hesperitin or naringenin frequently modified nodule development, most markedly under saline conditions. Flavonoid additions had only small effects under high nitrogen availability conditions, and stronger effects under saline and low root zone temperature conditions.

scholarly journals SOYBEAN SEEDLING ROOT GROWTH PROMOTION BY 1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-PRODUCING PSEUDOMONADS

2016 ◽  
Vol 10 (1) ◽  
pp. 19 ◽  
Author(s):  
Edi Husen ◽  
Aris Tri Wahyudi ◽  
Antonius Suwanto ◽  
Rasti Saraswati
Keyword(s):  
Root Growth ◽  
Higher Plants ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Ethylene Biosynthesis ◽  
Soil Biology ◽  
Soybean Root ◽  
Soybean Seedlings ◽  
Local Soil ◽  
Growth Room

Pseudomonad producing 1-aminocyclopropane-1-carboxylate (ACC) deaminase (E.C.4.1.99.4) has been known to promote plant growth by lowering ethylene biosynthesis in higher plants, which can be induced by indole-3-acetic acid (IAA) production. The objective of this study was to examine the ability of IAAproducing Pseudomonas isolated from local soil environment (rhizosphere of soybean grown in Plumbon's agricultural area<br />in Cirebon, West Java, Indonesia) to promote soybean root growth in relation to their ACC deaminase activities. The experiments were conducted in growth room and Laboratory of Soil Biology Research, Indonesian Soil Research Institute, Bogor, from January to August 2008. Soybean seeds were inoculated by immersing the seeds for 1 hour in bacterial cell suspension containing approximately 108-109 cells ml-1. The seeds were then germinated<br />for 2 days before planting in growth pouches containing sterilized distilled water. All treated and untreated seeds were grown for 7 days in growth room at 24°C with 1300 lux of light intensity for 12-hour followed by a 12-hour dark period at 22°C. ACC deaminase activity of the isolates was assayed based on their ability to grow in Dworkin-Foster’s salt minimal medium containing ammonium sulfate or ACC as a source of nitrogen. Thirteen out of 81 isolates tested significantly increased soybean root length and weight, up to 50% from untreated plants. Of 13 isolates, 11 demonstrated ACC deaminase activities. Two isolates that did not show ACC deaminase activities had lower capacity to produce IAA. The results suggest that the effectiveness of IAA producing Pseudomonas in promoting the growth of the soybean seedlings is associated with their ACC deaminase activities or they produce IAA at low levels.

scholarly journals Ameliorating the Drought Stress for Wheat Growth through Application of ACC-Deaminase Containing Rhizobacteria along with Biogas Slurry

2020 ◽  
Vol 12 (15) ◽  
pp. 6022 ◽  
Author(s):  
Rizwan Yaseen ◽  
Omar Aziz ◽  
Muhammad Hamzah Saleem ◽  
Muhammad Riaz ◽  
Muhammad Zafar-ul-Hye ◽  
...  
Keyword(s):  
Drought Stress ◽  
Field Experiment ◽  
Higher Plants ◽  
Acc Deaminase ◽  
Ethylene Biosynthesis ◽  
Control Treatment ◽  
Biogas Slurry ◽  
Wheat Growth ◽  
Relative Water ◽  
Water Holding

The temperature increase around the world is leading to generation of drought, which is a big threat to the productivity of crops. Abiotic stresses like drought increase the ethylene level in plants. In higher plants, 1-aminocyclopropane-1-carboxylate (ACC) is considered as the immediate precursor of ethylene biosynthesis. The application of ACC-deaminase (ACCD) possessing rhizobacteria could ameliorate the harmful results of drought stress by transforming ACC into non-harmful products. Biogas slurry (BGS) improves the water-holding capacity and structure of the soil. Thus, we speculated that the integrated application of ACCD possessing rhizobacteria and BGS might be an efficient approach to mitigate the drought stress for better wheat productivity. A field experiment was conducted under skipped irrigation situations. On the tillering stage (SIT) and flowering stage (SIF), the irrigations were skipped, whereas the recommended four irrigations were maintained in the control treatment. The results of this field experiment exposed that the ACCD possessing rhizobacterial inoculations with BGS considerably improved the stomatal and sub-stomatal conductance, transpiration and photosynthetic rates up to 98%, 46%, 38%, and 73%, respectively, compared to the respective uninoculated controls. The Pseudomonas moraviensis with BGS application improved the grain yield and plant height up to 30.3% and 24.3%, respectively, where irrigation was skipped at the tillering stage, as compared to the uninoculated controls. The data obtained revealed that the P. moraviensis inoculation + BGS treatment significantly increased the relative water content (RWC), catalase (CAT) activity, ascorbate peroxidase (APX) activity, as well as grain and shoot phosphorus contents, up to 37%, 40%, 75%, 19%, and 84%, respectively, at SIF situation. The results depicted that the P. moraviensis with BGS application under drought stress could be applied for enhancing the physiological, yield, and growth attributes of wheat.

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