SOYBEAN SEEDLING ROOT GROWTH PROMOTION BY 1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-PRODUCING PSEUDOMONADS

Pseudomonad producing 1-aminocyclopropane-1-carboxylate (ACC) deaminase (E.C.4.1.99.4) has been known to promote plant growth by lowering ethylene biosynthesis in higher plants, which can be induced by indole-3-acetic acid (IAA) production. The objective of this study was to examine the ability of IAAproducing Pseudomonas isolated from local soil environment (rhizosphere of soybean grown in Plumbon's agricultural area<br />in Cirebon, West Java, Indonesia) to promote soybean root growth in relation to their ACC deaminase activities. The experiments were conducted in growth room and Laboratory of Soil Biology Research, Indonesian Soil Research Institute, Bogor, from January to August 2008. Soybean seeds were inoculated by immersing the seeds for 1 hour in bacterial cell suspension containing approximately 108-109 cells ml-1. The seeds were then germinated<br />for 2 days before planting in growth pouches containing sterilized distilled water. All treated and untreated seeds were grown for 7 days in growth room at 24°C with 1300 lux of light intensity for 12-hour followed by a 12-hour dark period at 22°C. ACC deaminase activity of the isolates was assayed based on their ability to grow in Dworkin-Foster’s salt minimal medium containing ammonium sulfate or ACC as a source of nitrogen. Thirteen out of 81 isolates tested significantly increased soybean root length and weight, up to 50% from untreated plants. Of 13 isolates, 11 demonstrated ACC deaminase activities. Two isolates that did not show ACC deaminase activities had lower capacity to produce IAA. The results suggest that the effectiveness of IAA producing Pseudomonas in promoting the growth of the soybean seedlings is associated with their ACC deaminase activities or they produce IAA at low levels.

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SOYBEAN SEEDLING ROOT GROWTH PROMOTION BY 1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE-PRODUCING PSEUDOMONADS

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v10n1.2009.p19-25 ◽

2016 ◽

Vol 10 (1) ◽

pp. 19 ◽

Cited By ~ 6

Author(s):

Edi Husen ◽

Aris Tri Wahyudi ◽

Antonius Suwanto ◽

Rasti Saraswati

Keyword(s):

Root Growth ◽

Higher Plants ◽

Growth Promotion ◽

Acc Deaminase ◽

Ethylene Biosynthesis ◽

Soil Biology ◽

Soybean Root ◽

Soybean Seedlings ◽

Local Soil ◽

Growth Room

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Rhizobium leguminosarum Biovar viciae 1-Aminocyclopropane-1-Carboxylate Deaminase Promotes Nodulation of Pea Plants

Applied and Environmental Microbiology ◽

10.1128/aem.69.8.4396-4402.2003 ◽

2003 ◽

Vol 69 (8) ◽

pp. 4396-4402 ◽

Cited By ~ 196

Author(s):

Wenbo Ma ◽

Frèdèrique C. Guinel ◽

Bernard R. Glick

Keyword(s):

Pisum Sativum ◽

Rhizobium Leguminosarum ◽

Higher Plants ◽

Regulatory Gene ◽

Regulatory Protein ◽

Acc Deaminase ◽

Ethylene Biosynthesis ◽

Plant Roots ◽

Nodule Development ◽

Insertion Mutants

ABSTRACT Ethylene inhibits nodulation in various legumes. In order to investigate strategies employed by Rhizobium to regulate nodulation, the 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene was isolated and characterized from one of the ACC deaminase-producing rhizobia, Rhizobium leguminosarum bv. viciae 128C53K. ACC deaminase degrades ACC, the immediate precursor of ethylene in higher plants. Through the action of this enzyme, ACC deaminase-containing bacteria can reduce ethylene biosynthesis in plants. Insertion mutants with mutations in the rhizobial ACC deaminase gene (acdS) and its regulatory gene, a leucine-responsive regulatory protein-like gene (lrpL), were constructed and tested to determine their abilities to nodulate Pisum sativum L. cv. Sparkle (pea). Both mutants, neither of which synthesized ACC deaminase, showed decreased nodulation efficiency compared to that of the parental strain. Our results suggest that ACC deaminase in R. leguminosarum bv. viciae 128C53K enhances the nodulation of P. sativum L. cv. Sparkle, likely by modulating ethylene levels in the plant roots during the early stages of nodule development. ACC deaminase might be the second described strategy utilized by Rhizobium to promote nodulation by adjusting ethylene levels in legumes.

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The critical period of aluminum stress on soybean root growth

Buletin Agroteknologi ◽

10.32663/ba.v1i1.1279 ◽

2020 ◽

Vol 1 (1) ◽

pp. 21

Author(s):

Danner Sagala ◽

Eka Suzanna ◽

Prihanani Prihanani

Keyword(s):

Root Growth ◽

Critical Threshold ◽

Aluminum Stress ◽

Considerable Effort ◽

Aluminum Exposure ◽

Soybean Root ◽

Soybean Seedlings ◽

Soybean Roots ◽

Tidal Swamps ◽

The Impact

Aluminum is prevalent in soils of tidal swamps. Soybean is known to be very sensitive to aluminum stress and so when tidal swamps are converted to soybean cropland, considerable effort and expense are required to overcome Al toxicity in soybean roots. It is therefore necessary to determine at what time in early development soybeans can best endure aluminum stress and identify aluminum-tolerant cultivars. This study was conducted by testing the impact of aluminum exposure on three soybean cultivars (Tanggamus, Karasumame, and M652) (relative to no-exposure controls) at four time periods at 10, 20, and 30 days after planting. No significant effect of aluminum on root growth in the first five days after exposure was observed, but the toxic effects became evident after soybeans had been exposed to aluminum for 10 days. Soybean seedlings that experienced aluminum stress earliest (at 10 days after planting) were more negatively impacted by Al exposure than seedlings exposed later (e.g., 30 days after planting). Root growths of the three cultivars we tested in this study were all detrimentally impacted by aluminum exposure. However, the M652 cultivar was the most sensitive to aluminum exposure. We conclude that the critical threshold period for soybean root growth to succumb to aluminum stress is within the first 30 days after planting, whereas the tolerance to aluminum stress occurs only during the first 10 days of exposure.

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The Role of Rhizobial ACC Deaminase in the Nodulation Process of Leguminous Plants

International Journal of Agronomy ◽

10.1155/2016/1369472 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 21

Author(s):

Francisco X. Nascimento ◽

Clarisse Brígido ◽

Bernard R. Glick ◽

Márcio J. Rossi

Keyword(s):

Stress Responses ◽

Higher Plants ◽

Growth Promotion ◽

Agricultural Practices ◽

Acc Deaminase ◽

Nodule Development ◽

Nodule Formation ◽

Direct Role ◽

Sustainable Agricultural Practices

Symbiotic rhizobia-legumes associations are extremely important in terms of sustainable agricultural practices. This symbiosis involves a complex interaction between both partners, plant and bacterium, for bacterial infection and the formation of symbiotic N-fixing nodules. In this regard, the phytohormone ethylene plays a significant role in nodule formation, acting as an inhibitor of the nodulation process. Ethylene not only regulates nodule development but also regulates many other plant developmental cues, including various stress responses that inhibit overall plant growth. Some rhizobia produce the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, thus, being able to decrease ACC and, consequently, decrease deleterious ethylene levels that affect the nodulation process. This occurs because ACC is the immediate precursor of ethylene in all higher plants. Hence, rhizobia that express this enzyme have an increased symbiotic potential. In addition to the direct role that ACC deaminase plays in the nodulation processper se, in a limited number of instances, ACC deaminase can also modulate nodule persistence. This review focuses on the important role of rhizobial ACC deaminase during the nodulation process, emphasizing its significance to legume growth promotion.

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Ameliorating the Drought Stress for Wheat Growth through Application of ACC-Deaminase Containing Rhizobacteria along with Biogas Slurry

Sustainability ◽

10.3390/su12156022 ◽

2020 ◽

Vol 12 (15) ◽

pp. 6022 ◽

Cited By ~ 4

Author(s):

Rizwan Yaseen ◽

Omar Aziz ◽

Muhammad Hamzah Saleem ◽

Muhammad Riaz ◽

Muhammad Zafar-ul-Hye ◽

...

Keyword(s):

Drought Stress ◽

Field Experiment ◽

Higher Plants ◽

Acc Deaminase ◽

Ethylene Biosynthesis ◽

Control Treatment ◽

Biogas Slurry ◽

Wheat Growth ◽

Relative Water ◽

Water Holding

The temperature increase around the world is leading to generation of drought, which is a big threat to the productivity of crops. Abiotic stresses like drought increase the ethylene level in plants. In higher plants, 1-aminocyclopropane-1-carboxylate (ACC) is considered as the immediate precursor of ethylene biosynthesis. The application of ACC-deaminase (ACCD) possessing rhizobacteria could ameliorate the harmful results of drought stress by transforming ACC into non-harmful products. Biogas slurry (BGS) improves the water-holding capacity and structure of the soil. Thus, we speculated that the integrated application of ACCD possessing rhizobacteria and BGS might be an efficient approach to mitigate the drought stress for better wheat productivity. A field experiment was conducted under skipped irrigation situations. On the tillering stage (SIT) and flowering stage (SIF), the irrigations were skipped, whereas the recommended four irrigations were maintained in the control treatment. The results of this field experiment exposed that the ACCD possessing rhizobacterial inoculations with BGS considerably improved the stomatal and sub-stomatal conductance, transpiration and photosynthetic rates up to 98%, 46%, 38%, and 73%, respectively, compared to the respective uninoculated controls. The Pseudomonas moraviensis with BGS application improved the grain yield and plant height up to 30.3% and 24.3%, respectively, where irrigation was skipped at the tillering stage, as compared to the uninoculated controls. The data obtained revealed that the P. moraviensis inoculation + BGS treatment significantly increased the relative water content (RWC), catalase (CAT) activity, ascorbate peroxidase (APX) activity, as well as grain and shoot phosphorus contents, up to 37%, 40%, 75%, 19%, and 84%, respectively, at SIF situation. The results depicted that the P. moraviensis with BGS application under drought stress could be applied for enhancing the physiological, yield, and growth attributes of wheat.

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Screening, Identification and Growth-Promotion Products of Multifunctional Bacteria in a Chinese Fir Plantation

Forests ◽

10.3390/f12020120 ◽

2021 ◽

Vol 12 (2) ◽

pp. 120

Author(s):

Guangyu Zhao ◽

Yihui Wei ◽

Jiaqi Chen ◽

Yuhong Dong ◽

Lingyu Hou ◽

...

Keyword(s):

High Efficiency ◽

Growth Promotion ◽

Nitrogenase Activity ◽

Acc Deaminase ◽

Inorganic Phosphorus ◽

Chinese Fir ◽

Biochemical Tests ◽

Growth Promoting ◽

Chinese Fir Plantation ◽

Physiological And Biochemical

Purpose: This research was aimed to screen and identify multifunctional phosphorus-dissolving bacteria of a Chinese fir (Cunninghamia lanceolata) plantation and study its phosphorus-dissolving characteristics in order to provide strain resources and a theoretical basis for developing the appropriate bacterial fertilizer of a Chinese fir plantation. Methods: First, phosphorus-dissolving bacteria were isolated from the woodland soil of a Chinese fir plantation by Pikovskava inorganic phosphorus medium (PVK). Then, some growth-promoting indicators of primary screening strains were determined, including the capacity of phosphorus-solubilized, nitrogenase activity, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, production of indole-3-acetic acid (IAA), secretion of iron carrier and so on. Finally, the screening multifunctional phosphorus-dissolving bacteria were identified, which were combined with colony characteristics, physiological and biochemical tests and molecular biotechnology. Results: (1) Thirteen phosphorus-dissolving bacteria were isolated and screened in total, and P5 (195.61 mg·L−1) had the strongest capacity of phosphorus-solubilized. Five phosphorus-dissolving bacteria were provided with nitrogenase activity, and the highest activity of nitrogenase was P10 and P5 (71.90 C2H4 nmol·mL−1·h−1 and 71.00 C2H4 nmol·mL−1·h−1, respectively). Four strains were provided with ACC deaminase activity, and the highest activity of ACC deaminase was P5 and P9, (0.74 μmol·mg−1·h−1 and 0.54 μmol·mg−1·h−1, respectively). Most strains could secrete IAA, and three strains of bacteria had a strong secretory ability, which could secrete IAA with a concentration greater than 15 mg·mL−1, and P5 was 18.00, P2 was 17.30, P6 was 15.59 (mg·mL−1). P5 produced carriers of iron better than others, and the ratio of the diameter of the iron production carrier ring to the diameter of the colony was 1.80, respectively, which was significantly higher than other strains. Combining all kinds of factors, P5 multifunctional phosphorus-dissolving bacteria were screened for eventual further study. (2) Strain P5 was identified as Burkholderia ubonensis, based on the colony characteristics, physiological and biochemical tests, 16SrDNA sequence analysis and phylogenetic tree construction. Conclusion: P5 has a variety of high-efficiency growth-promoting capabilities, and the ability to produce IAA, ACC deaminase activity and siderophore performance are significantly higher than other strains, which had great potential in the development of microbial fertilizer.

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