scholarly journals Role of Capsular Colanic Acid in Adhesion of Uropathogenic Escherichia coli

2003 ◽  
Vol 69 (8) ◽  
pp. 4474-4481 ◽  
Author(s):  
Andrea Hanna ◽  
Michael Berg ◽  
Valerie Stout ◽  
Anneta Razatos
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Bacterial Adhesion ◽  
Specific Binding ◽  
Binding Interactions ◽  
Colanic Acid ◽  
Mutant Strains ◽  
Tract Infections ◽  
Repulsive Forces

ABSTRACT Urinary tract infections are the most common urologic disease in the United States and one of the most common bacterial infections of any organ system. Biofilms persist in the urinary tract and on catheter surfaces because biofilm microorganisms are resistant to host defense mechanisms and antibiotic therapy. The first step in the establishment of biofilm infections is bacterial adhesion; preventing bacterial adhesion represents a promising method of controlling biofilms. Evidence suggests that capsular polysaccharides play a role in adhesion and pathogenicity. This study focuses on the role of physiochemical and specific binding interactions during adhesion of colanic acid exopolysaccharide mutant strains. Bacterial adhesion was evaluated for isogenic uropathogenic Escherichia coli strains that differed in colanic acid expression. The atomic force microscope (AFM) was used to directly measure the reversible physiochemical and specific binding interactions between bacterial strains and various substrates as bacteria initially approach the interface. AFM results indicate that electrostatic interactions were not solely responsible for the repulsive forces between the colanic acid mutant strains and hydrophilic substrates. Moreover, hydrophobic interactions were not found to play a significant role in adhesion of the colanic acid mutant strains. Adhesion was also evaluated by parallel-plate flow cell studies in comparison to AFM force measurements to demonstrate that prolonged incubation times alter bacterial adhesion. Results from this study demonstrate that the capsular polysaccharide colanic acid does not enhance bacterial adhesion but rather blocks the establishment of specific binding as well as time-dependent interactions between uropathogenic E. coli and inert substrates.

scholarly journals An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium

2017 ◽  
Vol 118 ◽  
pp. 64-69 ◽  
Author(s):  
Andreia S. Azevedo ◽  
Carina Almeida ◽  
Luciana C. Gomes ◽  
Carla Ferreira ◽  
Filipe J. Mergulhão ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
In Vitro Model ◽  
Microbial Consortium ◽  
Vitro Model ◽  
Tract Infections

scholarly journals Role of Motility in the Colonization of Uropathogenic Escherichia coli in the Urinary Tract

2005 ◽  
Vol 73 (11) ◽  
pp. 7644-7656 ◽  
Author(s):  
M. Chelsea Lane ◽  
Virginia Lockatell ◽  
Greta Monterosso ◽  
Daniel Lamphier ◽  
Julia Weinert ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Wild Type Strain ◽  
Uropathogenic Escherichia Coli ◽  
Wild Type ◽  
Upec Strain ◽  
Host Immune Responses ◽  
Strain Cft073 ◽  
Tract Infections

ABSTRACT Uropathogenic Escherichia coli (UPEC) causes most uncomplicated urinary tract infections (UTIs) in humans. Flagellum-mediated motility and chemotaxis have been suggested to contribute to virulence by enabling UPEC to escape host immune responses and disperse to new sites within the urinary tract. To evaluate their contribution to virulence, six separate flagellar mutations were constructed in UPEC strain CFT073. The mutants constructed were shown to have four different flagellar phenotypes: fliA and fliC mutants do not produce flagella; the flgM mutant has similar levels of extracellular flagellin as the wild type but exhibits less motility than the wild type; the motAB mutant is nonmotile; and the cheW and cheY mutants are motile but nonchemotactic. Virulence was assessed by transurethral independent challenges and cochallenges of CBA mice with the wild type and each mutant. CFU/ml of urine or CFU/g bladder or kidney was determined 3 days postinoculation for the independent challenges and at 6, 16, 48, 60, and 72 h postinoculation for the cochallenges. While these mutants colonized the urinary tract during independent challenge, each of the mutants was outcompeted by the wild-type strain to various degrees at specific time points during cochallenge. Altogether, these results suggest that flagella and flagellum-mediated motility/chemotaxis may not be absolutely required for virulence but that these traits contribute to the fitness of UPEC and therefore significantly enhance the pathogenesis of UTIs caused by UPEC.

scholarly journals Involvement of biofilm-like in tracellular bacterial communities of uropathogenic Escherichia coli in pathogenesis of urinary tract infections – a mini review

2013 ◽  
Vol 26 (3) ◽  
pp. 321-325
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Bacterial Communities ◽  
Uropathogenic Escherichia Coli ◽  
E Coli ◽  
Etiologic Agents ◽  
Tract Infections ◽  
Intracellular Bacterial Communities

This paper presents a precisely defined question about the role of the biofilm-like intracellular bacterial communities in pathogenesis of the urinary tract infections. According to the recent literature, uropathogenic Escherichia coli is one of the leading etiologic agents of the urinary tract infections. Although E. coli is regarded as an extracellular pathogen, some experiments have revealed a multi-step infection cycle, which involves adhesion, invasion, proliferation within invaded urothelial cell in the form of biofilm-like intracellular bacterial communities and dispersal, leading to infection of next neighbouring cells. Therefore, the prevention and treatment of the urinary tract infections must include intracellular stage of infection.

scholarly journals Adhesion of Escherichia Coli to Nanostructured Surfaces and the Role of Type 1 Fimbriae

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2247
Author(s):  
Pawel Kallas ◽  
Håvard J Haugen ◽  
Nikolaj Gadegaard ◽  
John Stormonth-Darling ◽  
Mats Hulander ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Virulence Factor ◽  
Wild Type ◽  
E Coli ◽  
Type 1 Fimbriae ◽  
Tract Infections

Bacterial fimbriae are an important virulence factor mediating adhesion to both biotic and abiotic surfaces and facilitating biofilm formation. The expression of type 1 fimbriae of Escherichia coli is a key virulence factor for urinary tract infections and catheter-associated urinary tract infections, which represent the most common nosocomial infections. New strategies to reduce adhesion of bacteria to surfaces is therefore warranted. The aim of the present study was to investigate how surfaces with different nanotopography-influenced fimbriae-mediated adhesion. Surfaces with three different nanopattern surface coverages made in polycarbonate were fabricated by injection molding from electron beam lithography nanopatterned templates. The surfaces were constructed with features of approximately 40 nm width and 25 nm height with 100 nm, 250 nm, and 500 nm interspace distance, respectively. The role of fimbriae type 1-mediated adhesion was investigated using the E. coli wild type BW25113 and ΔfimA (with a knockout of major pilus protein FimA) and ΔfimH (with a knockout of minor protein FimH) mutants. For the surfaces with nanotopography, all strains adhered least to areas with the largest interpillar distance (500 nm). For the E. coli wild type, no difference in adhesion between surfaces without pillars and the largest interpillar distance was observed. For the deletion mutants, increased adhesion was observed for surfaces without pillars compared to surfaces with the largest interpillar distance. The presence of a fully functional type 1 fimbria decreased the bacterial adhesion to the nanopatterned surfaces in comparison to the mutants.

scholarly journals Common anti-hemostatic medications increase the severity of uropathogenic Escherichia coli sepsis

2021 ◽  
Author(s):  
Vi Tran ◽  
Elinor Hortle ◽  
Warwick J Britton ◽  
Stefan H Oehlers
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Aminocaproic Acid ◽  
Uropathogenic Escherichia Coli ◽  
Sepsis Model ◽  
Tract Infections

AbstractUropathogenic Escherichia coli (UPEC) causes urinary tract infections that can result in sepsis. Hemostasis is protective in the pyelonephritis stage of ascending UPEC infection, the role of hemostasis but has not been investigated during sepsis. Here we utilize a zebrafish-UPEC sepsis model to visualize infection-induced coagulation and examine the effects of commonly prescribed anti-hemostatic medications on the infection severity. Treatment of septic zebrafish with warfarin, aspirin, or ticagrelor reduced host survival, while stabilization of clots with aminocaproic acid increased host survival. Our findings provide evidence that commonly prescribed anti-hemostatic medications may worsen the outcome of severe UPEC infection.

scholarly journals The IrgA Homologue Adhesin Iha Is an Escherichia coli Virulence Factor in Murine Urinary Tract Infection

2005 ◽  
Vol 73 (2) ◽  
pp. 965-971 ◽  
Author(s):  
James R. Johnson ◽  
Srdjan Jelacic ◽  
Laura M. Schoening ◽  
Connie Clabots ◽  
Nurmohammad Shaikh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Virulence Factor ◽  
Deletion Mutants ◽  
E Coli ◽  
Uroepithelial Cells ◽  
Strain Cft073 ◽  
Tract Infections ◽  
Gene Homologue

ABSTRACT The role of the Escherichia coli iron-regulated gene homologue adhesin (Iha) in the pathogenesis of urinary tract infections (UTIs) is unknown. We performed a series of complementary analyses to confirm or refute the hypothesis that Iha is a virulence factor in uropathogenic E. coli. Fecal E. coli isolates exhibited significantly lower prevalences of iha (range, 14 to 22%) than did clinical isolates from cases of pediatric cystitis or pyelonephritis, adult pyelonephritis or urosepsis, or bacteremia (range, 38 to 74%). Recombinant Iha from E. coli pyelonephritis isolate CFT073 conferred upon nonadherent E. coli ORN172 the ability to adhere to cultured T-24 human uroepithelial cells. In a well-established mouse model of ascending UTI, CFT073 and its derivative UPEC76 (a pap [P fimbriae] mutant version of strain CFT073) each significantly outcompeted their respective iha deletion mutants in CBA/J mice 48 h after bladder challenge (P < 0.03 for urine, both kidneys, and bladders of both constructs, except for bladders of mice challenged with UPEC76 and its deletion mutant, where P = 0.11). These data suggest that IhaCFT073 is a virulence factor and might be a target for anti-UTI interventions.

Aqueous Root Extract from Ononis spinosa Exerts Anti-adhesive Activity against Uropathogenic Escherichia coli

Planta Medica ◽  
2020 ◽  
Vol 86 (04) ◽  
pp. 247-254 ◽  
Author(s):  
Melanie Deipenbrock ◽  
Jandirk Sendker ◽  
Andreas Hensel
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Cell Viability ◽  
Urinary Tract Infections ◽  
Bacterial Adhesion ◽  
Uropathogenic Escherichia Coli ◽  
E Coli ◽  
T24 Cells ◽  
Bladder Cells ◽  
Tract Infections

AbstractExtracts from Ononis spinosa are traditionally used for urinary tract infections due to diuretic and anti-inflammatory activity. A potential influence on the virulence of uropathogenic Escherichia coli has not been investigated until now. The following study aimed to investigate the influence of an aqueous O. spinosa extract on uropathogenic E. coli and uropathogenic E. coli host cell interaction. A hot water extract from the roots of O. spinosa (O. spinosa extract) was characterized by LC-qTOF-MS. The influence of O. spinosa extract on the proliferation of uropathogenic E. coli UTI89 and on cell viability against human T24 bladder cells was investigated. Anti-adhesive activity of O. spinosa extract was assessed by flow cytometry, evaluating the adhesion of fluorescent-labelled UTI89 to T24 bladder cells. Internalization of uropathogenic E. coli into T24 cells was monitored by an invasion assay. O. spinosa extract was characterized by the presence of isoflavones, isoflavanones, licoagrosides, pterocarpans, tartaric acid derivatives, and saponines. O. spinosa extract had no influence on the proliferation of uropathogenic E. coli (125 – 1000 µg/mL) and did not influence the cell viability of T24 cells. Bacterial adhesion to T24 cells was significantly (p > 0.001) inhibited by O. spinosa extract in a concentration-dependent manner (125 – 1000 µg/mL) during coincubation. Preincubation of uropathogenic E. coli or T24 cells with O. spinosa extract reduced bacterial adhesion, but to a lower extent than during coincubation. Consequently, the reduced bacterial adhesion also leads to a reduced internalization of uropathogenic E. coli uropathogenic E. coli into the host cell. O. spinosa extract does not interact with FimH-mediated uropathogenic E. coli adhesion to host cells. From these data, the traditional use of O. spinosa extracts for urinary tract infections seems to be rationalized.

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