The Metabolic Pathway of 4-Aminophenol in Burkholderia sp. Strain AK-5 Differs from That of Aniline and Aniline with C-4 Substituents
2003 ◽
Vol 69
(9)
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pp. 5410-5413
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Keyword(s):
ABSTRACT Burkholderia sp. strain AK-5 utilized 4-aminophenol as the sole carbon, nitrogen, and energy source. A pathway for the metabolism of 4-aminophenol in strain AK-5 was proposed based on the identification of three key metabolites by gas chromatography-mass spectrometry analysis. Strain AK-5 converted 4-aminophenol to 1,2,4-trihydroxybenzene via 1,4-benzenediol. 1,2,4-Trihydroxybenzene 1,2-dioxygenase cleaved the benzene ring of 1,2,4-trihydroxybenzene to form maleylacetic acid. The enzyme showed a high dioxygenase activity only for 1,2,4-trihydroxybenzene, with Km and V max values of 9.6 μM and 6.8 μmol min−1 mg of protein−1, respectively.
2006 ◽
2016 ◽
Vol 18
(4)
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pp. 365-369
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2012 ◽
Vol 425
(2)
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pp. 183-188
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