scholarly journals Immune Responses to Bile-Tolerant Helicobacter Species in Patients with Chronic Liver Diseases, a Randomized Population Group, and Healthy Blood Donors

2002 ◽  
Vol 9 (6) ◽  
pp. 1160-1164 ◽  
Author(s):  
Olga Ananieva ◽  
Ingrid Nilsson ◽  
Tamara Vorobjova ◽  
Raivo Uibo ◽  
Torkel Wadström
Keyword(s):  
Cell Surface ◽  
Liver Diseases ◽  
Blood Donors ◽  
Population Group ◽  
Cross Reactivity ◽  
Chronic Liver ◽  
Antibody Responses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chronic Liver Diseases ◽  
H Pylori

ABSTRACT Bile-tolerant Helicobacter species such as Helicobacter pullorum, Helicobacter bilis, and Helicobacter hepaticus are associated with hepatic disorders in animals and may be involved in the pathogenesis of chronic liver diseases (CLD) in humans. Antibody responses to cell surface proteins of H. pullorum, H. bilis, and H. hepaticus in serum samples from patients with CLD, a randomized population group, and healthy blood donors were evaluated by using enzyme linked immunosorbent assay (ELISA). The results were compared with the antibody responses to Helicobacter pylori. For analysis of a possible cross-reactivity between bile-tolerant Helicobacter species and H. pylori, sera from a subpopulation of each group were absorbed with a whole-cell extract of H. pylori and retested by ELISA. Results before absorption showed that the mean value of the ELISA units for H. pullorum was significantly higher in patients with CLD than in healthy blood donors (P = 0.01). Antibody reactivity to cell surface protein of H. hepaticus was also significantly higher in the CLD patients than in the healthy blood donors and the population group (P = 0.005 and P = 0.002, respectively). Following the absorption, antibody responses to H. pullorum decreased significantly in all three groups (P = 0.0001 for CLD patients, P = 0.0005 for the population group, and P < 0.0001 for the blood donors), indicating that cross-reactivity between H. pylori and other Helicobacter spp. occurs. The antibody responses to H. hepaticus and H. bilis in CLD patients remained high following absorption experiments compared to ELISA results before absorption. The significance of this finding requires further investigations.

scholarly journals Cryptosporidiosis in patients with diarrhea and chronic liver diseases

2014 ◽  
Vol 8 (12) ◽  
pp. 1584-1590 ◽  
Author(s):  
Nasser Mousa ◽  
Ahmed Abdel-Razik ◽  
Hala El-Nahas ◽  
Atef El-Shazly ◽  
Mohammad Abdelaziz ◽  
...  
Keyword(s):  
Hepatic Encephalopathy ◽  
Liver Diseases ◽  
Meld Score ◽  
Chronic Liver ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cryptosporidium Infection ◽  
Chronic Liver Diseases ◽  
Pugh Class ◽  
Class C ◽  
Precipitating Factor

Introduction: The aim of this study was to evaluate the epidemiology and clinical significance of Cryptosporidium in patients with diarrhea and chronic liver diseases. Methodology: The study included 150 patients with chronic liver diseases and diarrhea, and 50 subjects with diarrhea as a control group. Stool samples were screened for the presence of Cryptosporidium by microscopic examination after modified Ziehl-Neelsen staining and detection of Cryptosporidium coproantigen by enzyme-linked immunosorbent assay (ELISA). Results: The prevalence of Cryptosporidium infection in patients with chronic liver diseases was 30% (45/150) versus 14% (7/50) in controls. Cryptosporidium infection increased with the progression of chronic liver diseases from Child-Pugh class A to Child-Pugh class C (p<  0.001) and from model for end-stage liver disease (MELD) score ≤ 9 to MELD score > 9 (p< 0.031). Nine patients in Child-Pugh class C with diarrhea associated with Cryptosporidium infection developed hepatic encephalopathy, and only diarrhea was identified as a precipitating factor for hepatic encephalopathy. Conclusions: Cryptosporidium is one of the important causes of diarrhea in patients with chronic liver diseases. The infection significantly increased with the progression of chronic liver diseases. In patients with advanced chronic liver diseases, Cryptosporidium infection may be a precipitating factor of hepatic encephalopathy.

scholarly journals Prevalence and Clinical Significance of Immunoglobulin A Antibodies against Tissue Transglutaminase in Patients with Diverse Chronic Liver Diseases

2005 ◽  
Vol 12 (8) ◽  
pp. 941-948 ◽  
Author(s):  
Anastasios E. Germenis ◽  
Efthalia E. Yiannaki ◽  
Kalliopi Zachou ◽  
Violeta Roka ◽  
Sotirios Barbanis ◽  
...  
Keyword(s):  
Liver Disease ◽  
Clinical Significance ◽  
Liver Diseases ◽  
Tissue Transglutaminase ◽  
Immunoglobulin A ◽  
Chronic Liver ◽  
Intestinal Biopsy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chronic Liver Diseases ◽  
Hla Dq

ABSTRACT The prevalence of celiac disease (CD) and the prevalence and clinical significance of anti-tissue transglutaminase (tTG) antibodies (tTGAbs) in a large series of patients with chronic liver diseases were assessed. We studied 738 patients (462 with chronic viral hepatitis, 117 with autoimmune liver diseases, 113 with alcoholic or nonalcoholic fatty liver disease, and 46 with other liver disorders) and 1,350 healthy controls (HC). Immunoglobulin A (IgA) tTGAbs were measured by enzyme-linked immunosorbent assay and a microsphere-based flow cytometric assay. Positive sera were investigated for IgA antiendomysial antibodies (EmA). IgA tTGAb-positive subjects were invited to undergo a small-intestinal biopsy and HLA-DQ allele typing. Four of 1,350 HC (0.3%) tested tTGAb+ EmA+ and underwent a biopsy (CD confirmation in all). Four of 738 liver disease patients tested tTGAbs+ EmA+ (0.54%; not statistically significant). Two were HCV infected (1.24%; not statistically significant), and two had transaminasemia of unknown origin. Forty-three patients tested tTGAbs+ EmA− (5.8%; P < 0.001 compared to HC). Inhibition experiments verified the existence of specific IgA anti-tTG reactivity. Twenty-six of 43 patients underwent a biopsy (all negative for CD). Binary logistic regression analysis revealed age (P = 0.008), cirrhosis (P = 0.004), alkaline phosphatase (P = 0.026), and antinuclear antibodies (P = 0.012) as independent risk factors for tTGAb reactivity among the patients. It was concluded that CD prevalence is the same in HC and patients with chronic liver diseases. The prevalence of tTGAbs is higher in hepatic patients compared to HC, but their specificity for CD diagnosis in this group of patients is low. tTGAbs in patients appear to be associated with the presence of autoimmunity, cirrhosis, and cholestasis, irrespective of the origin of the liver disease.

Detection of Anti-LKM-1(Anti-CYP2D6) by an: Enzyme-Linked Immunosorbent Assay in Adult Patients with Chronic Liver Diseases

Autoimmunity ◽  
1999 ◽  
Vol 30 (2) ◽  
pp. 107-114 ◽  
Author(s):  
Hiroshi Miyakawa ◽  
Eriko Kikazawa ◽  
Kazuhiro Abe ◽  
Kentaro Kikuchi ◽  
Hirotoshi Fujikawa ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Liver Diseases ◽  
Chronic Liver ◽  
Adult Patients ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chronic Liver Diseases

Magnitude of hepatitis C virus infection in India: Prevalence in healthy blood donors, acute and chronic liver diseases

1997 ◽  
Vol 51 (3) ◽  
pp. 167-174 ◽  
Author(s):  
Aswini K. Panigrahi ◽  
Subrat K. Panda ◽  
Rajesh K. Dixit ◽  
Kanury V. S. Rao ◽  
Subrat K. Acharya ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
Liver Diseases ◽  
Blood Donors ◽  
Chronic Liver ◽  
Hepatitis C Virus Infection ◽  
Chronic Liver Diseases

scholarly journals Host Response to the Presence of Helicobacter spp. DNA in the Liver of Patients with Chronic Liver Diseases

2011 ◽  
Vol 60 (2) ◽  
pp. 175-178
Author(s):  
MAGDA RYBICKA ◽  
JOANNA NAKONIECZNA ◽  
PIOTR STALKE ◽  
KRZYSZTOF PIOTR BIELAWSKI
Keyword(s):  
Gene Expression ◽  
Hepatocellular Carcinoma ◽  
Liver Diseases ◽  
Host Response ◽  
Chronic Liver ◽  
Expression Level ◽  
Chronic Liver Diseases ◽  
H Pylori ◽  
Prevalent Species

Literature data indicate an association between the presence of Helicobacter spp. in the liver and the development of hepatocellular carcinoma (HCC). However, the role of H. pylori infections in chronic liver diseases (CLD) remains controversial. The aim of this study was to detect Helicobacter spp. DNA in patients with CLD, and to investigate the host response to the presence of the bacterium in the liver. Helicobacter spp. DNA was detected in 59% samples. H.pylori was the most prevalent species (94%). We estimated the expression level of IL-1 and IL-8 genes. The presence of Helicobacter spp. did not have a significant effect on the gene expression of IL-8 and IL-1.

scholarly journals Prevalence and Phylogenetic Analysis of TT Virus DNA in Western India

2000 ◽  
Vol 7 (5) ◽  
pp. 845-849 ◽  
Author(s):  
V. A. Arankalle ◽  
S. S. Gandhe ◽  
T. M. Deshmukh ◽  
M. S. Chadha ◽  
A. M. Walimbe
Keyword(s):  
Phylogenetic Analysis ◽  
Chronic Hepatitis ◽  
Liver Diseases ◽  
Blood Donors ◽  
Chronic Liver ◽  
Western India ◽  
Chronic Liver Diseases ◽  
Tt Virus ◽  
Genotype 1A

ABSTRACT In western India, TT virus (TTV) DNA positivity varied from 6.7% (5 of 75) in chronic hepatitis patients to 24.4% (10 of 41) in hemophiliacs; 7.4% (4 of 54) of voluntary blood donors had circulating TTV DNA. Phylogenetic analysis revealed a predominance of genotype 1a. In India, TTV is transmitted mainly by nonparenteral routes and is not an important cause of chronic liver diseases.

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