scholarly journals Time to Detection of Mycobacterium tuberculosis Using the MGIT 320 System Correlates with Colony Counting in Preclinical Testing of New Vaccines

2013 ◽  
Vol 21 (3) ◽  
pp. 453-455 ◽  
Author(s):  
K. Kolibab ◽  
A. Yang ◽  
M. Parra ◽  
S. C. Derrick ◽  
S. L. Morris
Keyword(s):  
Culture System ◽  
Liquid Culture ◽  
Preclinical Testing ◽  
Content Type ◽  
Solid Media ◽  
Liquid Culture System ◽  
Colony Counting ◽  
Liquid Systems ◽  
Time To Detection ◽  
New Vaccines

ABSTRACTClinical studies have suggested that the enumeration of mycobacteria by using automated liquid systems is a faster and simpler alternative to quantitative cultures. Here, we show that the time to detection ofM. tuberculosisgrowth as measured with the MGIT 320 liquid culture system inversely correlates with CFU determinations from culture on solid media and that mycobacterial quantification using the MGIT system is faster and easier to perform than CFU plating.

scholarly journals Effect of Egg Yolk on the Detection of Mycobacterium Avium Subsp. Paratuberculosis Using the ESP II Liquid Culture System

2005 ◽  
Vol 17 (6) ◽  
pp. 554-560 ◽  
Author(s):  
N. Beth Harris ◽  
Suelee Robbe-Austerman ◽  
Janet B. Payeur
Keyword(s):  
Mycobacterium Avium ◽  
Culture System ◽  
Liquid Culture ◽  
Egg Yolk ◽  
Mycobacterium Avium Subsp ◽  
Culture Methods ◽  
Solid Media ◽  
Liquid Culture System ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Time To Detection

Rapid diagnosis of paratuberculosis in infected cattle is important for the successful control of Johne disease within herds. Thus, improving culture methods for Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) will aid in the identification of asymptomatic animals. Egg yolk is a component of the media used for growing M. paratuberculosis, but its requirement as a supplement has not been reported. Using the ESP II liquid culture system, 2 different sources and 5 concentrations (3.3%, 1.6%, 0.8%, 0.4%, and 0%) of egg yolk were analyzed. Egg yolk source did not affect either recovery rate or time to detection, but both parameters were significantly improved when the 3.3% egg yolk concentrations (final volume) were used over media containing no egg yolk. This study also assessed the recovery of M. paratuberculosis from fecal samples that were cultured multiple times using Herrold egg yolk agar (HEY). Specimens containing greater than 70 cfu/g feces could routinely be identified as positive for M. paratuberculosis after only 1 culture attempt, whereas specimens with fewer bacteria were only intermittently positive, even after 5 replicate cultures. Therefore, this study indicates that the sensitivity of the Trek Diagnostic ESP II liquid culture system for M. paratuberculosis is affected by egg yolk concentration and that single culture attempts using HEY solid media may not identify specimens containing low numbers of bacteria.

scholarly journals Comparison of MGIT and Myco/F Lytic Liquid-Based Blood Culture Systems for Recovery of Mycobacterium tuberculosis from Pleural Fluid

2015 ◽  
Vol 53 (4) ◽  
pp. 1391-1394 ◽  
Author(s):  
Elizabeth Harausz ◽  
John Kafuluma Lusiba ◽  
Mary Nsereko ◽  
John L. Johnson ◽  
Zahra Toossi ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pleural Fluid ◽  
Culture System ◽  
Liquid Culture ◽  
Content Type ◽  
Indicator Tube ◽  
Liquid Culture System ◽  
Mycobacterial Growth ◽  
Mycobacterial Growth Indicator Tube ◽  
Growth Indicator

The specificities and sensitivities of the Bactec mycobacterial growth indicator tube (MGIT) system for the recovery ofMycobacterium tuberculosisfrom pleural fluid are not statistically different than those of the Myco/F lytic liquid culture system. The time to positivity is shorter in the MGIT system (12.7 versus 20.7 days, respectively;P= 0.007). The Myco/F lytic culture system may be an alternative to the MGIT system for diagnosing pleural tuberculosis.

scholarly journals A liquid culture system for murine megakaryocyte progenitor cells

Blood ◽  
1982 ◽  
Vol 59 (2) ◽  
pp. 250-257
Author(s):  
T Nagasawa ◽  
M Nakazawa ◽  
T Abe
Keyword(s):  
Progenitor Cells ◽  
Conditioned Medium ◽  
Culture System ◽  
Liquid Culture ◽  
Culture Medium ◽  
Pokeweed Mitogen ◽  
Plasma Clot ◽  
Liquid Culture System ◽  
Cell Conditioned Medium ◽  
Number Of Cells

A liquid culture system is described for murine megakaryocyte progenitor cells (CFU-M) in the presence of pokeweed-mitogen-stimulated spleen-cell conditioned medium. There were dose-related responses between the number of CFU-M developed and the number of cells cultured and the dosage of conditioned medium in this liquid culture system. Murine CFU-M were abundantly cloned in this system an the plating efficiency was similar in comparison with that in a plasma clot system. The acetylcholinesterase-positive colonies (more than 4 acetylcholinesterase-positive cells) were clearly seen on day 3 of culture, and they reached a maximum (60.5 +/- 10.7/2 x 10(5) cells) on day 7 of culture. Ultrastructural analyses of megakaryocytic maturation in this system showed that a few megakaryocytes produced platelets that were released in the culture medium on day 5 of culture. This liquid culture system is suitable for the study of the dynamic process of the megakaryocyte-platelet system.

scholarly journals Effect of gangliosides on murine megakaryocytopoiesis in a liquid culture system.

1988 ◽  
Vol 13 (4) ◽  
pp. 293-300
Author(s):  
Yasuko Watanabe ◽  
Masami Taniguchi ◽  
Nobuko Fukamachi ◽  
Bonro Kobayashi
Keyword(s):  
Culture System ◽  
Liquid Culture ◽  
Liquid Culture System

scholarly journals Liquid Culture System for Mature Pollen in Hyuganatsu (Citrus tamurana hort. ex Tanaka)

2012 ◽  
Vol 11 (2) ◽  
pp. 173-179 ◽  
Author(s):  
Asuka Uchida ◽  
Akiha Abe ◽  
Yoichiro Hoshino ◽  
Hisato Kunitake
Keyword(s):  
Culture System ◽  
Liquid Culture ◽  
Mature Pollen ◽  
Liquid Culture System
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