scholarly journals Anti-Interleukin-15 Prevents Arthritis in Borrelia-Vaccinated and -Infected Mice

2006 ◽  
Vol 13 (2) ◽  
pp. 289-296 ◽  
Author(s):  
Corey A. Amlong ◽  
Dean T. Nardelli ◽  
Sara Heil Peterson ◽  
Thomas F. Warner ◽  
Steven M. Callister ◽  
...  
Keyword(s):  
Lymph Node ◽  
Significant Role ◽  
Inflammatory Cells ◽  
Interleukin 17 ◽  
Memory Cells ◽  
Present Evidence ◽  
Receptor Alpha ◽  
Lymph Node Cells ◽  
Interleukin 15 ◽  
Stimulation Of

ABSTRACT We showed previously that interleukin-17 (IL-17) plays a significant role in the induction of arthritis associated with Borrelia vaccination and challenge. Little information, however, is available about the chain of immunologic events that leads to the release of IL-17. The production of IL-17 has been linked to stimulation of memory cells by IL-15. Therefore, we hypothesized that IL-15 is involved in the induction of arthritis associated with Borrelia vaccination and infection of mice. Here we present evidence that treatment of Borrelia-vaccinated and -infected mice with anti-IL-15 antibody prevents swelling of the hind paws. More importantly, both anti-IL-15 antibody- and recombinant IL-15 receptor alpha-treated Borrelia-vaccinated and -infected mice were free of major histopathologic indications of arthritis, including hyperplasia, hypertrophy, and vilus formation of the synovium. Similarly, the synovial space and perisynovium were free of inflammatory cells. By contrast, the synovium of nontreated Borrelia-vaccinated and -infected mice had overt hyperplasia, hypertrophy, and vilus formation. Moreover, the synovial space and perisynovium were infiltrated with neutrophils, macrophages, and lymphocytes. Finally, we show that recombinant IL-15 stimulates the release of IL-17 from lymph node cells obtained near the arthritic site. These results suggest that IL-15 plays a major role in orchestrating IL-17 induction of arthritis associated with Borrelia-vaccinated and -infected mice.

scholarly journals HAPTEN CARRIER RELATIONSHIPS IN THE DNP-PLL·FOREIGN ALBUMIN COMPLEX SYSTEM: INDUCTION OF TOLERANCE AND STIMULATION OF CELLS IN VITRO

1968 ◽  
Vol 127 (1) ◽  
pp. 43-53 ◽  
Author(s):  
Ira Green ◽  
William E. Paul ◽  
Baruj Benacerraf
Keyword(s):  
Lymph Node ◽  
Cell Cultures ◽  
Thymidine Incorporation ◽  
Lymph Node Cell ◽  
Simple Order ◽  
Significant Stimulation ◽  
Lymph Node Cells ◽  
Stimulate Cell Proliferation ◽  
Stimulation Of

Genetic nonresponder guinea pigs made tolerant to BSA and then immunized with DNP-PLL·BSA failed to make anti-DNP-PLL antibodies. Thus, tolerance to a carrier protein renders animals unresponsive to the hapten which it bears. The addition in vitro of DNP-PLL or DNP-GL to lymph node cell cultures derived from genetic responder animals immunized with these materials led to a significant stimulation of 3H-thymidine incorporation into DNA. However, the addition of DNP-PLL or DNP-GL to lymph node cell cultures from nonresponder animals immunized with these materials failed to produce any stimulation of DNA synthesis. Furthermore, the addition of DNP-PLL to lymph node cell cultures from nonresponder animals immunized with DNP-PLL·BSA or DNP-PLL·OVA also failed to stimulate cell proliferation in spite of the fact that the lymph node cells of these animals were producing anti-DNP-PLL antibodies. The above facts suggest that the function of the PLL gene product is to act at an early crucial step in the immune mechanism to form an antigen-inducer complex. The specificity of this early step may be of a simple order and different than that of the antibody which is later produced in the immune response.

scholarly journals Macrophage migration inhibitory factor stimulates interleukin-17 expression and production in lymph node cells

Immunology ◽  
2009 ◽  
Vol 126 (1) ◽  
pp. 74-83 ◽  
Author(s):  
Ivana Stojanović ◽  
Tamara Cvjetićanin ◽  
Sandra Lazaroski ◽  
Stanislava Stošić-Grujičić ◽  
Djordje Miljković
Keyword(s):  
Lymph Node ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Interleukin 17 ◽  
Macrophage Migration ◽  
Lymph Node Cells

W1185 CpG Oligonucleotide Stimulation of TLR9 Inhibits Il17 Production from Mesenteric Lymph Node Cells

2008 ◽  
Vol 134 (4) ◽  
pp. A-651
Author(s):  
Ahmed Metwali ◽  
Sarah Winckler ◽  
M. Nedim Ince ◽  
David E. Elliott
Keyword(s):  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Cpg Oligonucleotide ◽  
Mesenteric Lymph ◽  
Lymph Node Cells ◽  
Stimulation Of

scholarly journals A QUANTITATIVE STUDY OF THE STIMULATION OF DNA SYNTHESIS IN LYMPH NODE CELL CULTURES BY ANTI-LYMPHOCYTE SERUM, ANTI-GAMMA GLOBULIN SERUM, SPECIFIC ANTIGEN, AND PHYTOHEMAGGLUTININ

1969 ◽  
Vol 129 (2) ◽  
pp. 295-313 ◽  
Author(s):  
John Foerster ◽  
Jean-Pierre Lamelin ◽  
Ira Green ◽  
Baruj Benacerraf
Keyword(s):  
Lymph Node ◽  
Guinea Pig ◽  
Gamma Globulin ◽  
Narrow Range ◽  
Specific Antigen ◽  
Lymph Node Cell ◽  
Wide Range ◽  
Lymph Node Cells ◽  
Stimulation Of

Rabbit anti-guinea pig lymphocyte serum is an efficient stimulus of the synthesis of DNA by guinea pig lymph node cells in vitro. The ability of ALS to stimulate lymphocytes is characterized by its lack of dependence on prior sensitization, the magnitude of the response it elicits, and the stimulation of all sensitive lymph node cells simultaneously within a very narrow range of ALS concentrations. In contrast to this homogeneous response to ALS, the stimulation of lymph node cells by antigen proceeds in graded fashion over a wide range of concentrations, thus reflecting the heterogeneity of the response of sensitized cells to antigen. PHA gives a response which is intermediate between that of ALS and antigen. ALS appears to have specificity for membrane determinants shared by lymphocytes but not found on other tissues. This specificity does not involve cell-bound gamma globulin. The serum activity mediating lymphocyte stimulation as well as cytotoxicity is readily removed by absorption with lymph node cells.

Sign in / Sign up

Export Citation Format

Share Document