scholarly journals Complete Genome Sequence of a Fusarium graminearum Double-Stranded RNA Virus in a Newly Proposed Family, Alternaviridae

2018 ◽  
Vol 6 (8) ◽  
Author(s):  
Hao He ◽  
Xiaoguang Chen ◽  
Pengfei Li ◽  
Dewen Qiu ◽  
Lihua Guo

ABSTRACT We describe here a double-stranded RNA mycovirus, termed Fusarium graminearum alternavirus 1 (FgAV1/AH11), from the isolate AH11 of the phytopathogenic fungus F. graminearum . Phylogenetic analysis showed that FgAV1/AH11 belongs to a newly proposed family, Alternaviridae . This is the first report of a mycovirus in the family Alternaviridae that infects F. graminearum .

2017 ◽  
Vol 5 (9) ◽  
Author(s):  
Pengfei Li ◽  
Xiaoguang Chen ◽  
Hao He ◽  
Dewen Qiu ◽  
Lihua Guo

ABSTRACT We describe a novel positive single-stranded RNA virus, termed Fusarium langsethiae hypovirus 1 (FlHV1), from the isolate AH32 of the phytopathogenic fungus Fusarium langsethiae. The properties of FlHV1 permit assignment to the genus Alphahypovirus in the family Hypoviridae. This is the first report of a mycovirus identified in F. langsethiae.


2021 ◽  
Author(s):  
Zeli Chen ◽  
Lu Chen ◽  
Rex Frimpong Anane ◽  
Zhe Wang ◽  
Like Gao ◽  
...  

Abstract Paris mitovirus 1 (ParMV1) is a positive-sense RNA virus isolated from diseased Paris polyphylla var. yunnanensis plants in Wenshan, Yunnan. The complete genome sequence of ParMV1 consists of 2,751 nucleotides with a genome structure typical of the mitoviruses. ParMV1 genome has a single open reading frame (ORF: 358-2,637 nt) that encodes RNA-dependent RNA polymerase (RdRp) with a molecular mass of 86.42 kDa. ParMV1 contains six conserved motifs (Ι-VΙ) that are unique to mitoviruses. In addition, the 5′and 3′ terminals of the genome have a stable secondary structure, and the reverse complementary sequence forms a panhandle structure. Comparative genome analysis revealed that ParMV1 shares 23.1–40.6% amino acid (aa) and 32.3–45.7% nucleotide (nt) sequence identities with the RdRp of other mitoviruses. The phylogenetic tree inferred from RdRp aa sequence showed that ParMV1 clusters with mitoviruses, and hence should be considered as a new member of the genus Mitovirus in the family Motiviridae. This is the first report of a novel mitovirus infecting Paris polyphylla var. yunnanensis.


2017 ◽  
Vol 162 (7) ◽  
pp. 2119-2124 ◽  
Author(s):  
Luan Wang ◽  
Shuangchao Wang ◽  
Xiufen Yang ◽  
Hongmei Zeng ◽  
Dewen Qiu ◽  
...  

Author(s):  
Tobias Lutz ◽  
Gitta Langer ◽  
Cornelia Heinze

AbstractA novel dsRNA virus named “Thelonectria quadrivirus 1” (TQV1) was found in a member of the genus Thelonectria (Ascomycota), isolated from a root associated with stem collar necrosis of Fraxinus excelsior L. The complete genome of TQV1 is composed of four segments, each containing a single ORF on the positive sense RNA. The sequence of the 5´ (5´-(C/T)ACGAAAAA-3´) and 3´termini (5´AT(T/G)AGCAATG(T/C)GC(G/A)CG-3’) of dsRNA 1 (4876 bp), dsRNA 2 (4312 bp), dsRNA 3 (4158 bp), and dsRNA 4 (3933 bp) are conserved. Based on its genome organization and phylogenetic position, TQV1 is suggested to be a new member of the family Quadriviridae. This is the first report of a mycovirus infecting a member of the genus Thelonectria.


2016 ◽  
Vol 161 (12) ◽  
pp. 3571-3576 ◽  
Author(s):  
Caiyan Xin ◽  
Bin Wu ◽  
Jianhua Li ◽  
Pengtao Gong ◽  
Ju Yang ◽  
...  

2015 ◽  
Vol 3 (4) ◽  
Author(s):  
Bin Chen ◽  
Mark Bernards ◽  
Aiming Wang

Bell pepper endornavirus (BPEV) is a double-stranded RNA virus infecting economically important crops, such as peppers. Next-generation sequencing of small RNAs extracted from the leaves of a pepper plant showing mild viral symptoms, along with subsequent analysis, identified BPEV. The complete genome of this isolate was cloned and sequenced.


2011 ◽  
Vol 86 (2) ◽  
pp. 1282-1283 ◽  
Author(s):  
F. Villanueva ◽  
S. Sabanadzovic ◽  
R. A. Valverde ◽  
J. Navas-Castillo

2020 ◽  
Vol 9 (21) ◽  
Author(s):  
Yi-Ting Yang ◽  
Se Jin Lee ◽  
Yu-Shin Nai ◽  
Jae Su Kim

ABSTRACT A novel putative single-stranded RNA virus was discovered from the transcriptome of a bean bug, Riptortus pedestris, infected with the entomopathogenic fungus Beaveria bassiana JEF-007. The complete genome sequence was 9,915 nucleotides long and encoded a 2,916-amino-acid polyprotein. This virus belonged to Iflaviridae based on phylogenetic analysis and was named RiPV-2.


2016 ◽  
Vol 161 (10) ◽  
pp. 2899-2903 ◽  
Author(s):  
Xiaoguang Chen ◽  
Hao He ◽  
Xiufen Yang ◽  
Hongmei Zeng ◽  
Dewen Qiu ◽  
...  

Viruses ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 257
Author(s):  
E Meng ◽  
Baozhen Tang ◽  
Francisco Sanchez-Garcia ◽  
Ting Qiao ◽  
Lang Fu ◽  
...  

The complete sequence of a novel RNA virus isolated from Tetrastichus brontispae (TbRV-1) was determined to be 12,239 nucleotides in length with five non-overlapping, linearly arranged coding sequences (CDS), potentially encoding nucleoproteins, hypothetical proteins, matrix proteins, glycoproteins, and RNA-dependent RNA polymerases. Sequence analysis indicated that the RNA-dependent RNA polymerase of TbRV-1 shares a 65% nucleotide and 67% amino acid sequence identity with Hubei dimarhabdovirus 2, suggesting that TbRV-1 is a member of the dimarhabdovirus supergroup. This corresponded to the result of the phylogenetic analysis. The affiliation of TbRV-1 with members of the family Rhabdoviridae was further validated by similar transcription termination motifs (GGAACUUUUUUU) to the Drosophila sigmavirus. The prevalence of TbRV-1 in all tissues suggested that the virus was constitutive of, and not specific to, any wasp tissue. To our knowledge, this is the first report on the complete genome sequence of a dimarhabdovirus in parasitoids.


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